FastQCFastQC Report
Wed 25 May 2016
SRR1780489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71117
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT2823339.699368646034No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT1868426.272199333492697Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC51717.27111661065568No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG36855.181602148572071No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC30044.2240251979132974No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT17062.3988638440879115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC10221.437068492765443No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC8791.235991394462646No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG7441.0461633645963693No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC5330.7494691845831517No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG4510.6341662331088207No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4270.6004190277992604No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4110.5779208909262202No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3330.46824247367014915No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3190.448556603906239No Hit
CTTATCACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC2460.34590885442299313No Hit
ACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCT2320.32622298465908295Illumina PCR Primer Index 10 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT2110.2966941800132177No Hit
ATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCTGCTT1890.2657592418127874Illumina PCR Primer Index 10 (96% over 27bp)
CTTATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC1860.2615408411490923No Hit
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT1570.22076296806670695No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT950.13358268768367618No Hit
CTTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC810.11389681791976601No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGC790.111084550810636No Hit
CTCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG720.10124161592868092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCA302.4589917E-644.090911
CTTACAC302.4589917E-644.090911
TCTTTAT750.044.090911
CTTATAA800.044.0909081
CTTAACA407.9780875E-944.0909081
TCTTTAC207.727635E-444.0909081
TATAACA800.043.997893
TTATAAC800.043.997892
ATAACAC800.043.997894
TTATCAC302.495066E-643.9978872
TATCACA302.495066E-643.9978873
ATCACAT302.495066E-643.9978874
TCACATC302.495066E-643.9978875
CTTTACA5150.042.806711
TTTACAC5150.042.71642
CCGTCTT19950.042.56437344
CTATACA3450.039.617921
TTAACAC452.2990207E-839.1092382
GCCGTCT21900.038.77439543
TCTTAAT700.037.7922061
Produced by FastQC (version 0.11.5)