FastQCFastQC Report
Wed 25 May 2016
SRR1780488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12833
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC170.13247097327203305No Hit
CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG140.10909374269461544No Hit
CTTTACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGG130.10130133250214292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATG351.9736612E-549.99720825
GAATGTG305.664616E-446.66406227
CGAATGT305.664616E-446.66406226
AATGTGT305.664616E-446.66406228
CGCCGTA305.664616E-446.66406250
CGAACGA404.3454886E-543.7475622
ATGTGTG350.001210164539.9977729
GTGTGTA350.001210164539.9977731
TGTGTGT350.001210164539.9977730
AACGAAT458.705007E-538.8867224
GAACGAA458.705007E-538.8867223
TCTGACG951.5279511E-1036.840058
GTCGCCG400.002332091834.99804748
TCGCCGT400.002332091834.99804749
GGTCGCC400.002332091834.99804747
CTGACGC956.4756023E-933.1560449
GACGCTG956.4756023E-933.15604411
TGACGCT956.4756023E-933.15604410
CGCTGCC859.326868E-832.9393413
ACGAACG552.833376E-431.81640621
Produced by FastQC (version 0.11.5)