##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12833 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.87368503078002 12.0 12.0 23.0 12.0 32.0 2 18.236187952933843 12.0 11.0 23.0 11.0 32.0 3 19.096158341775112 21.0 11.0 23.0 11.0 32.0 4 18.496142756954725 11.0 11.0 23.0 11.0 33.0 5 19.55489752980597 21.0 11.0 27.0 11.0 33.0 6 21.610067793968675 21.0 11.0 31.0 11.0 36.0 7 22.264318553728668 23.0 11.0 34.0 11.0 36.0 8 22.47409023611003 23.0 11.0 34.0 11.0 36.0 9 22.124133094366087 21.0 12.0 33.0 12.0 36.0 10-11 20.80557936569781 21.0 11.0 31.0 11.0 35.0 12-13 20.60223642172524 21.0 11.0 29.5 11.0 34.0 14-15 20.34898309046988 21.0 11.0 29.5 11.0 34.0 16-17 19.824359074261668 16.5 11.0 27.5 11.0 34.0 18-19 20.076287695784305 21.0 11.0 28.0 11.0 34.0 20-21 20.410387282786566 21.0 11.0 29.5 11.0 34.0 22-23 19.723408400218187 16.0 11.0 27.5 10.5 34.0 24-25 19.82202135120393 16.0 11.0 27.5 10.5 34.0 26-27 19.50771448609055 16.0 11.0 27.0 10.5 34.0 28-29 18.998402555910545 11.0 11.0 26.0 11.0 34.0 30-31 18.971090158185927 11.0 11.0 26.0 11.0 34.0 32-33 19.417790072469415 11.0 11.0 26.5 11.0 34.0 34-35 19.688030857944362 16.0 11.0 27.0 11.0 34.0 36-37 19.231824203226058 11.0 11.0 26.0 11.0 34.0 38-39 18.718031637185383 11.0 11.0 26.0 11.0 34.0 40-41 19.08096314189979 11.0 11.0 26.0 11.0 34.0 42-43 19.34843762175641 16.0 11.0 26.5 11.0 34.0 44-45 19.10176887711369 11.0 11.0 26.0 11.0 34.0 46-47 19.242188108782045 16.0 11.0 26.5 11.0 34.0 48-49 19.449894802462403 16.0 11.0 26.5 11.0 34.0 50-51 19.57406685887945 16.0 11.0 27.0 11.0 34.0 52-53 19.386659393750485 15.0 11.0 26.5 11.0 34.0 54-55 19.547962284734666 20.5 11.0 26.5 11.0 34.0 56-57 18.860554819605703 15.0 11.0 26.0 11.0 33.5 58-59 18.964973116184836 16.0 11.0 26.0 11.0 33.5 60-61 19.254928699446737 19.0 11.0 26.0 11.0 33.0 62-63 18.8469960258708 15.0 11.0 26.0 11.0 33.0 64-65 19.016831606015742 15.0 11.0 26.0 11.0 33.0 66-67 19.434855450790927 19.0 11.0 26.0 11.0 33.0 68-69 19.21207044338814 19.0 11.0 26.0 11.0 33.0 70-71 19.67139406218343 19.0 11.0 26.0 11.0 33.5 72-73 18.84828177355256 15.0 11.0 25.0 11.0 33.0 74-75 18.483908672952545 11.0 11.0 25.0 11.0 32.5 76 17.412062650977948 11.0 11.0 24.0 11.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6430350184856302 1101 2 2.6015463141408652 1101 3 1.6921109870652522 1101 4 1.9329600117435675 1101 5 -0.6055101850127116 1101 6 -2.23206321284761 1101 7 0.280767674992898 1101 8 -4.1293391800068235 1101 9 -1.2765035189639526 1101 10-11 -0.27258627459001517 1101 12-13 0.8126636984406481 1101 14-15 -0.40255192864809075 1101 16-17 1.7020490142474785 1101 18-19 -1.2876452266937228 1101 20-21 0.08558611277658557 1101 22-23 -1.19090572502434 1101 24-25 -0.9162736263693603 1101 26-27 -0.5509351195254624 1101 28-29 2.4421686203727617 1101 30-31 -0.1939083280127889 1101 32-33 -1.1170928284991106 1101 34-35 -0.981514157181369 1101 36-37 1.3750666689400326 1101 38-39 0.9278489946664372 1101 40-41 -0.7312149290293561 1101 42-43 -1.4811337378117457 1101 44-45 0.002031269519711998 1101 46-47 -1.606620058866934 1101 48-49 -0.8989421292345376 1101 50-51 -1.4167751420928063 1101 52-53 -0.8823112878499906 1101 54-55 0.22163836517736968 1101 56-57 -1.7892502995229407 1101 58-59 -0.32377283643505805 1101 60-61 -0.8567278994856835 1101 62-63 0.8537401853046447 1101 64-65 -2.6443980054668863 1101 66-67 -2.247628964890918 1101 68-69 2.260520228250339 1101 70-71 -3.6099207826319386 1101 72-73 -0.5799873907040158 1101 74-75 -0.33881713967825533 1101 76 -0.5422602997641235 1102 1 -1.0757149815143698 1102 2 -2.1172036858591348 1102 3 1.0358609870652522 1102 4 -0.31703998825643254 1102 5 -3.4180101850127116 1102 6 -0.9820632128476099 1102 7 0.905767674992898 1102 8 0.12066081999317646 1102 9 2.2859964810360474 1102 10-11 -0.6788362745900152 1102 12-13 -1.9842113015593519 1102 14-15 -2.8244269286480908 1102 16-17 -0.6417009857525215 1102 18-19 -1.8501452266937228 1102 20-21 0.14808611277658557 1102 22-23 -0.59715572502434 1102 24-25 -1.1193986263693603 1102 26-27 0.9490648804745376 1102 28-29 -1.8703313796272383 1102 30-31 -1.522033328012789 1102 32-33 -0.4764678284991106 1102 34-35 -0.38776415718136903 1102 36-37 0.2969416689400326 1102 38-39 -1.6659010053335628 1102 40-41 -1.6062149290293561 1102 42-43 -0.21550873781174573 1102 44-45 -1.982343730480288 1102 46-47 0.33087994113306607 1102 48-49 -1.3208171292345376 1102 50-51 0.3644748579071937 1102 52-53 1.2895637121500094 1102 54-55 -1.4658616348226303 1102 56-57 -1.1798752995229407 1102 58-59 -0.38627283643505805 1102 60-61 0.1276471005143165 1102 62-63 -1.5837598146953553 1102 64-65 -1.0037730054668863 1102 66-67 0.267996035109082 1102 68-69 0.5417702282503392 1102 70-71 0.12445421736806139 1102 72-73 -0.6424873907040158 1102 74-75 -0.5731921396782553 1102 76 -1.5735102997641235 1103 1 -0.8211418107826631 1103 2 0.5687719238969642 1103 3 0.7683304992603759 1103 4 1.6768624507679561 1103 5 2.0461666442555817 1103 6 -1.9165144323598042 1103 7 2.4210115774319227 1103 8 0.5642583809687878 1103 9 2.4475818468897046 1103 10-11 1.5193344571172993 1103 12-13 -0.15151313082764517 1103 14-15 0.5631492908641036 1103 16-17 0.7919880386377223 1103 18-19 2.385754163550178 1103 20-21 2.398467210337561 1103 22-23 1.2629814700976105 1103 24-25 -0.7455419190522861 1103 26-27 -0.2872156073303387 1103 28-29 0.16396740086056738 1103 30-31 -1.387124791427425 1103 32-33 -0.12204709679179615 1103 34-35 -0.6122306205960015 1103 36-37 -0.6093083310599674 1103 38-39 -0.5934924687481988 1103 40-41 0.9486631197511315 1103 42-43 0.1674943109687419 1103 44-45 0.4768788304953233 1103 46-47 1.3686085996696526 1103 48-49 -0.489643348746732 1103 50-51 -0.8584672152635378 1103 52-53 0.7560271267841578 1103 54-55 -1.3713494397006798 1103 56-57 -2.242756397083916 1103 58-59 -0.9708764949716446 1103 60-61 -0.29841997265641496 1103 62-63 -0.1923726195734048 1103 64-65 0.009565409167262118 1103 66-67 -0.37034237952506643 1103 68-69 -2.2047998937008835 1103 70-71 -0.3705915143392531 1103 72-73 -2.003767878508892 1103 74-75 -2.009929944556305 1103 76 0.7931055538944136 1104 1 -1.3667864100857976 1104 2 -0.39220368585913334 1104 3 0.988539558493823 1104 4 -1.0813257025421485 1104 5 1.5918112435587162 1104 6 2.20543678715239 1104 7 -0.8263751821499596 1104 8 1.2938751057074604 1104 9 1.6252821953217627 1104 10-11 1.817592296838555 1104 12-13 1.588556555583505 1104 14-15 -0.48737335721952135 1104 16-17 -0.63902241432395 1104 18-19 -0.5389845124080104 1104 20-21 1.302996827062298 1104 22-23 0.05775498926137246 1104 24-25 0.283726373630639 1104 26-27 0.2901363090459661 1104 28-29 2.828775763229906 1104 30-31 1.6163595291300688 1104 32-33 -0.5095035427848273 1104 34-35 0.6055394142472004 1104 36-37 1.1335488117971728 1104 38-39 0.7099918518092956 1104 40-41 -0.7276435004579298 1104 42-43 3.034044833616825 1104 44-45 -0.23636158762314352 1104 46-47 1.502754941133066 1104 48-49 1.946593585051179 1104 50-51 0.97742128647862 1104 52-53 0.21456371215001013 1104 54-55 1.1662812223202295 1104 56-57 0.4812854147627732 1104 58-59 -0.3335942650064858 1104 60-61 0.8088971005143151 1104 62-63 -0.6118848146953546 1104 64-65 -1.146183719752603 1104 66-67 0.22826389225194177 1104 68-69 -0.5497476288925185 1104 70-71 -0.8545636397747955 1104 72-73 -0.9264159621325874 1104 74-75 1.2705578603217447 1104 76 -1.2208317283355505 1105 1 0.5203216038514853 1105 2 -0.26049636878596516 1105 3 -0.060937793422553455 1105 4 -2.176796085817408 1105 5 -1.6367601850127116 1105 6 1.0103148359328777 1105 7 -3.6765493981778334 1105 8 -2.7284245458604808 1105 9 -1.0402230311590763 1105 10-11 -1.0660313965412342 1105 12-13 -0.49297654546179004 1105 14-15 -1.4856311969407763 1105 16-17 -2.2567924491671576 1105 18-19 0.007705383062376114 1105 20-21 0.008223307898536092 1105 22-23 0.8239570798537095 1105 24-25 -0.1357858214913108 1105 26-27 -2.165264387818148 1105 28-29 -2.104325282066263 1105 30-31 -1.411515035329863 1105 32-33 -1.1098519748405735 1105 34-35 -1.6854013523033196 1105 36-37 -0.17028394081606635 1105 38-39 -1.2886144199677112 1105 40-41 -0.9659710265903314 1105 42-43 -1.393481298787357 1105 44-45 -2.693852876821751 1105 46-47 -2.911879205208397 1105 48-49 -0.9652531048442938 1105 50-51 -0.395052581117195 1105 52-53 1.0243198097109847 1105 54-55 -1.2615933421397045 1105 56-57 0.34260945657462116 1105 58-59 0.18766009039420695 1105 60-61 -2.286224850705196 1105 62-63 -1.3265189610368182 1105 64-65 -0.8684833713205471 1105 66-67 -1.041074086842137 1105 68-69 0.13666352093326495 1105 70-71 -2.480347611900232 1105 72-73 -0.979377634606454 1105 74-75 0.2461676164193065 1105 76 -1.8654310314714397 1106 1 -0.5205679226908408 1106 2 0.8313257259055717 1106 3 0.5229933400064297 1106 4 -1.0376282235505485 1106 5 -3.2544072438362406 1106 6 2.0289661989170966 1106 7 -0.498644089712986 1106 8 -0.33889800353623656 1106 9 -2.0595917542580686 1106 10-11 0.26969313717469134 1106 12-13 -0.4979980662652359 1106 14-15 -1.4705666345304458 1106 16-17 -2.066333338693699 1106 18-19 0.9173180086003931 1106 20-21 -1.2407006519292985 1106 22-23 1.028763392622718 1106 24-25 0.9954910795129948 1106 26-27 -2.0178468842313464 1106 28-29 -1.3611402031566513 1106 30-31 -0.6231362691892599 1106 32-33 -2.0334531226167556 1106 34-35 0.38705201928921795 1106 36-37 -0.44754362517761237 1106 38-39 -1.1824451229806208 1106 40-41 -0.34150904667641413 1106 42-43 -0.9462072672235102 1106 44-45 -0.646865789303817 1106 46-47 -1.4531274118081114 1106 48-49 -1.4118097762933601 1106 50-51 -0.7687972009163353 1106 52-53 -1.8207304054970486 1106 54-55 -0.046743987763804284 1106 56-57 -0.8830002995229407 1106 58-59 -0.6638463658468226 1106 60-61 -2.16463231125039 1106 62-63 -0.014825991165942298 1106 64-65 -1.6113097701727703 1106 66-67 -1.146526023714447 1106 68-69 -2.1484871246908384 1106 70-71 -0.4738913708672321 1106 72-73 -0.7785168024687188 1106 74-75 -0.5676774337959003 1106 76 -1.351083829175888 1107 1 1.0986271237487877 1107 2 -0.25536158059597724 1107 3 -0.9460469076715903 1107 4 -0.5407241987827476 1107 5 -1.0314970271179753 1107 6 -1.3998263707423462 1107 7 0.530767674992898 1107 8 -0.4023654957962961 1107 9 0.9109964810360474 1107 10-11 -0.8169941693268576 1107 12-13 -1.3370073541909306 1107 14-15 -1.4272229812796695 1107 16-17 -0.3341351962788366 1107 18-19 0.45988766804311965 1107 20-21 0.36765848119764044 1107 22-23 -0.3775833566032851 1107 24-25 0.45214742626222204 1107 26-27 1.3141964594219075 1107 28-29 1.3122344098464467 1107 30-31 1.3644798298819474 1107 32-33 0.5942558557114168 1107 34-35 1.0867424217660009 1107 36-37 0.3775337742031901 1107 38-39 -0.23662468954409022 1107 40-41 1.6043113867601164 1107 42-43 1.6652478411356242 1107 44-45 2.878676006361818 1107 46-47 -0.3393503220248313 1107 48-49 2.0270447128707225 1107 50-51 3.1909551210650875 1107 52-53 0.9540373963605369 1107 54-55 0.3828225757036847 1107 56-57 0.07752601626653188 1107 58-59 0.11866137409125699 1107 60-61 0.0010023636722138463 1107 62-63 0.7986415010941172 1107 64-65 2.0759967313752163 1107 66-67 -0.04203685962776049 1107 68-69 -0.19260477174966084 1107 70-71 -0.7447892036845687 1107 72-73 -0.0734084433355946 1107 74-75 2.0468736497954296 1107 76 -0.4501550366062297 1108 1 0.8617850184856302 1108 2 0.11036041670496743 1108 3 0.022239192193456603 1108 4 -2.310629731846177 1108 5 0.4658039175513906 1108 6 -0.7689221872065843 1108 7 -1.9948733506481275 1108 8 -0.49953148769913014 1108 9 1.6802272502668174 1108 10-11 0.010266289512546933 1108 12-13 0.6083367753637248 1108 14-15 -2.2254685953147586 1108 16-17 -0.27711765241918584 1108 18-19 -2.1173727907962885 1108 20-21 -2.2112888872234144 1108 22-23 -1.5462743147679312 1108 24-25 0.05808534798961418 1108 26-27 -1.815358196448539 1108 28-29 -1.4576711232169828 1108 30-31 1.156652569423109 1108 32-33 0.7150385817572982 1108 34-35 -0.6710173623095734 1108 36-37 -0.39135961311124845 1108 38-39 -0.7548433130258694 1108 40-41 -0.29211236492679404 1108 42-43 -1.0348196352476435 1108 44-45 1.4002684490068908 1108 46-47 -1.6126296742515471 1108 48-49 -1.534358795901202 1108 50-51 -1.997303988246653 1108 52-53 1.4017431993294984 1108 54-55 -2.9626565066175026 1108 56-57 -0.6458208123434517 1108 58-59 1.7026694712572485 1108 60-61 0.7422304338476522 1108 62-63 -0.3093207121312531 1108 64-65 0.3604096868408071 1108 66-67 -1.4391353751473268 1108 68-69 0.11508753594264576 1108 70-71 -0.2461387313498875 1108 72-73 0.49373055801393306 1108 74-75 1.7705578603217447 1108 76 0.05389354638972321 1109 1 1.0617850184856295 1109 2 0.20779631414086452 1109 3 1.8171109870652522 1109 4 2.1329600117435668 1109 5 2.7382398149872884 1109 6 2.2304367871523887 1109 7 3.0057676749928994 1109 8 2.958160819993175 1109 9 1.5109964810360488 1109 10-11 0.8961637254099877 1109 12-13 1.7814136984406481 1109 14-15 4.6411980713519085 1109 16-17 3.8145490142474756 1109 18-19 2.6842297733062743 1109 20-21 -0.1987888872234116 1109 22-23 -0.3565307250243386 1109 24-25 -0.11627362636935956 1109 26-27 1.174064880474539 1109 28-29 1.2859186203727617 1109 30-31 0.3967166719872104 1109 32-33 3.017282171500888 1109 34-35 1.1966108428186324 1109 36-37 1.303191668940034 1109 38-39 0.6778489946664372 1109 40-41 -0.7062149290293576 1109 42-43 -0.04988373781174715 1109 44-45 0.6832812695197106 1109 46-47 0.34025494113306465 1109 48-49 -0.41769212923453836 1109 50-51 -0.951150142092807 1109 52-53 -0.21043628784999058 1109 54-55 -0.4158616348226296 1109 56-57 0.10449970047706003 1109 58-59 -0.7550228364350566 1109 60-61 -0.8286028994856842 1109 62-63 0.4631151853046447 1109 64-65 1.1431019945331151 1109 66-67 1.283621035109082 1109 68-69 0.8073952282503392 1109 70-71 0.6400792173680614 1109 72-73 0.8450126092959813 1109 74-75 -0.29194213967825533 1109 76 -0.06726029976412207 1110 1 -0.7493260926254806 1110 2 -3.4366481303035794 1110 3 -1.238444568490305 1110 4 -1.344817766034211 1110 5 1.5854620372095098 1110 6 1.2887701204857223 1110 7 -2.7470101027848806 1110 8 0.3637163755487336 1110 9 1.4109964810360474 1110 10-11 -0.053836274590015165 1110 12-13 -0.5519196348926876 1110 14-15 0.6356425157963521 1110 16-17 0.07010456980303559 1110 18-19 -0.05327022669372283 1110 20-21 -0.10017777611230372 1110 22-23 0.9740248305312171 1110 24-25 -0.6940514041471388 1110 26-27 0.49767599158564835 1110 28-29 -1.4085258240716811 1110 30-31 -3.1018944391238996 1110 32-33 -0.6257733840546678 1110 34-35 2.4507775094852953 1110 36-37 1.0156916689400326 1110 38-39 -0.4193732275557842 1110 40-41 -1.0923260401404704 1110 42-43 -1.2207170711450814 1110 44-45 0.3874479361863763 1110 46-47 -0.38613394775581966 1110 48-49 1.2156412040987945 1110 50-51 1.2932943023516366 1110 52-53 0.06734148992778799 1110 54-55 -1.3408616348226303 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1.7783104968982748 2116 60-61 0.34223043384765006 2116 62-63 0.8547818519713104 2116 64-65 1.151435327866448 2116 66-67 0.6544543684424156 2116 68-69 3.4740618949170035 2116 70-71 1.7359125507013928 2116 72-73 0.0783459426293156 2116 74-75 3.603891193655077 2116 76 2.607739700235875 2117 1 2.055333405582406 2117 2 2.6852156689795734 2117 3 1.4300142128717042 2117 4 1.9813471085177596 2117 5 1.3632398149872884 2117 6 -0.37520837413793373 2117 7 2.063025739509026 2117 8 -1.4499036961358556 2117 9 -2.766422873802661 2117 10-11 -0.263513693944855 2117 12-13 1.3217362790858083 2117 14-15 1.0863593616744893 2117 16-17 -0.6910961470428454 2117 18-19 0.027374934596597456 2117 20-21 -1.2112888872234144 2117 22-23 0.6563725007821084 2117 24-25 0.9869521800822554 2117 26-27 0.3422100417648579 2117 28-29 2.7697895881146977 2117 30-31 2.8237328010194673 2117 32-33 0.3047821715008894 2117 34-35 -0.1110504475039491 2117 36-37 0.7939174753916447 2117 38-39 1.5851070591825653 2117 40-41 0.4018495870996759 2117 42-43 -0.6373837378117457 2117 44-45 0.4949748179068081 2117 46-47 1.4382388121008098 2117 48-49 -0.39995019375066576 2117 50-51 0.010140180487841377 2117 52-53 -0.6983395136564425 2117 54-55 1.0542996554999498 2117 56-57 -1.6168712672648766 2117 58-59 -0.5727647719189299 2117 60-61 2.4411551650304446 2117 62-63 2.7655345401433564 2117 64-65 1.9261665106621457 2117 66-67 1.1646694222058578 2117 68-69 1.8557823250245313 2117 70-71 2.7025792173680614 2117 72-73 -1.3259551326394998 2117 74-75 3.2060417312894884 2117 76 1.9174171195907164 2118 1 3.6359785668727262 2118 2 -0.4760746536010707 2118 3 0.5913045354523483 2118 4 -0.24445934309514072 2118 5 -0.8948247011417436 2118 6 0.7538238839265823 2118 7 0.06302573950902612 2118 8 -1.5789359542003716 2118 9 -0.15351964799621243 2118 10-11 -3.2312556294287234 2118 12-13 -3.033102430591608 2118 14-15 0.7476496842551335 2118 16-17 -1.2394832438170411 2118 18-19 -0.7145605492743705 2118 20-21 0.014517564389489479 2118 22-23 -1.5049178217985322 2118 24-25 -2.2388542715306485 2118 26-27 -3.3674673775899784 2118 28-29 0.14075733005018165 2118 30-31 0.7592166719872111 2118 32-33 -3.5984436349507263 2118 34-35 -1.933631092665241 2118 36-37 -0.9641470407373873 2118 38-39 -0.12457036017227097 2118 40-41 -0.43686009031967643 2118 42-43 0.5884227138011582 2118 44-45 -1.763089698222224 2118 46-47 -1.029503123383062 2118 48-49 0.8097272256041705 2118 50-51 -1.0221178840282867 2118 52-53 0.7210153250532336 2118 54-55 1.8930093329193056 2118 56-57 -3.6652583640390706 2118 58-59 -1.7985712235318339 2118 60-61 -0.20400612529213547 2118 62-63 -4.282852556630839 2118 64-65 -1.6060915538539824 2118 66-67 -0.4482338036005977 2118 68-69 -0.7087338040077249 2118 70-71 0.4606437334970934 2118 72-73 0.996625512521792 2118 74-75 -2.0842808493556753 2118 76 0.3367719583003925 2119 1 1.2848619415625535 2119 2 1.8154886218331718 2119 3 2.817110987065252 2119 4 1.0098830886666441 2119 5 0.5940090457565184 2119 6 0.8977444794600835 2119 7 1.761536905762128 2119 8 1.615853127685483 2119 9 -0.6659265958870293 2119 10-11 1.311548340794598 2119 12-13 1.4352598522868014 2119 14-15 0.0181211482749859 2119 16-17 1.7357028604013252 2119 18-19 2.812114388690894 2119 20-21 1.731018805084279 2119 22-23 0.2357769672833534 2119 24-25 0.737572527476793 2119 26-27 -1.8538197349100756 2119 28-29 0.3628416972958384 2119 30-31 -0.010014097243555398 2119 32-33 0.362474479193196 2119 34-35 1.2071877658955543 2119 36-37 -0.667000638752274 2119 38-39 1.437464379281824 2119 40-41 0.3745543017398738 2119 42-43 0.8818470314190243 2119 44-45 3.1438581925966353 2119 46-47 -1.3433989050207806 2119 48-49 -2.752307513849921 2119 50-51 -1.2665347574774195 2119 52-53 0.24148678907308607 2119 54-55 -1.2254770194380171 2119 56-57 -1.4983849149075539 2119 58-59 -2.816561297973518 2119 60-61 2.08197402359124 2119 62-63 0.511192108381568 2119 64-65 -0.3895903131591929 2119 66-67 1.2980441120321586 2119 68-69 0.46124138209649246 2119 70-71 2.298733063521908 2119 72-73 -0.6472950830117092 2119 74-75 -0.42174983198594873 2119 76 -0.4461064536102768 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 2.0 12 31.0 13 219.0 14 578.0 15 973.0 16 1316.0 17 1537.0 18 1692.0 19 1589.0 20 1397.0 21 1073.0 22 718.0 23 453.0 24 367.0 25 222.0 26 145.0 27 116.0 28 109.0 29 59.0 30 67.0 31 52.0 32 39.0 33 25.0 34 21.0 35 8.0 36 7.0 37 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.031845634951761 26.135383167307243 31.61816613067692 36.214605067064085 2 18.047222005766383 23.36164575703265 33.52294864801683 25.068183589184134 3 4.4650510402867605 26.034442453050726 44.775188965947166 24.725317540715345 4 18.429050105197536 24.725317540715345 26.40847814228941 30.437154211797708 5 3.8338658146964857 50.931193018000464 21.436920439491935 23.79802072781111 6 5.641704979350113 32.56448219434271 11.688615288708798 50.10519753759838 7 4.379334528169563 35.252863710745736 10.691186784072313 49.67661497701239 8 3.927374737006156 49.52076677316294 11.875633133328138 34.67622535650277 9 8.314501675368192 34.38011376918881 10.488584119068028 46.816800436374976 10-11 6.459908049559729 32.78266967973194 23.104496220681057 37.652926050027276 12-13 6.288475025325334 28.512428894256992 23.688926985116495 41.51016909530117 14-15 11.63406841736149 32.155380659237906 17.875788981531986 38.33476194186862 16-17 15.690017922543442 31.61380815086106 17.864100366243278 34.832073560352214 18-19 11.536663289955582 29.205953401387045 17.003039039975064 42.2543442686823 20-21 9.709343099820774 29.599470116106914 18.378399438946467 42.31278734512585 22-23 18.199174004519598 29.689082833320345 14.91856931348866 37.19317384867139 24-25 13.01772842392363 36.271186440677965 14.225599064874341 36.48548607052406 26-27 10.067793968674511 36.651601340294555 18.89659471674589 34.38400997428504 28-29 13.43021896672641 31.641081586534714 22.870723914906883 32.05797553183199 30-31 13.656198862308111 30.88521779786488 24.526611080807292 30.931972259019712 32-33 9.20283643731006 37.4737006156004 21.561599002571498 31.761863944518037 34-35 9.791163406841736 34.680121561599 20.934309982077455 34.5944050494818 36-37 10.936647705135199 30.589106210550927 23.716200420790152 34.75804566352373 38-39 11.00677939686745 24.7954492324476 28.598145406374194 35.59962596431076 40-41 8.657367723836982 27.935790540014025 25.434426868230343 37.972414867918644 42-43 4.858567755006623 26.026650042858257 29.143614119847268 39.97116808228785 44-45 6.315748460998987 27.908517104340376 30.6514454920907 35.12428894256993 46-47 7.262526299384399 32.03459830125458 25.485077534481416 35.21779786487961 48-49 7.301488350346761 34.37232135899634 20.143380347541495 38.1828099431154 50-51 7.5508454765058834 32.24888958154757 18.908283332034596 41.29198160991194 52-53 6.378087742538767 31.81251461076911 20.840801059767784 40.968596586924335 54-55 6.806670303124757 31.208602820852487 19.060235330787812 42.924491545234936 56-57 10.660017143302424 31.43847892153043 17.552403958544378 40.34909997662277 58-59 9.074261669134263 30.690407543053066 18.401776669523883 41.83355411828879 60-61 6.366399127250058 28.18125146107691 19.640769890127014 45.811579521546015 62-63 8.49372710979506 30.024156471596662 17.910854827398115 43.57126159121016 64-65 8.94179069586223 32.31512506818358 14.906880698199954 43.83620353775422 66-67 6.87680199485701 39.047767474479855 12.226291591989401 41.84913893867373 68-69 9.058676848749318 39.164653627366945 11.614587391880308 40.16208213200343 70-71 6.374191537442531 42.71409647003818 10.722356424842204 40.18935556767708 72-73 9.074261669134263 40.524429205953396 12.502922153822176 37.89838697109016 74-75 9.035299618171901 38.872438245149226 15.69391412763968 36.398348009039196 76 10.488584119068028 34.395698589573755 17.89137380191693 37.22434348944129 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 1.0 11 0.0 12 0.0 13 1.5 14 5.0 15 6.5 16 5.0 17 4.0 18 4.0 19 9.5 20 13.0 21 11.0 22 16.5 23 23.0 24 30.0 25 36.0 26 41.0 27 51.0 28 67.5 29 79.0 30 93.0 31 125.0 32 154.5 33 166.0 34 200.5 35 281.0 36 329.0 37 331.0 38 364.5 39 439.0 40 504.5 41 585.5 42 642.0 43 691.0 44 756.5 45 825.5 46 878.0 47 854.5 48 808.0 49 793.0 50 801.0 51 763.0 52 681.5 53 604.0 54 570.0 55 519.5 56 435.5 57 363.0 58 324.0 59 300.5 60 259.5 61 208.0 62 174.0 63 143.5 64 109.5 65 93.5 66 67.5 67 54.0 68 47.5 69 33.0 70 24.5 71 24.0 72 15.0 73 9.0 74 9.5 75 7.0 76 5.0 77 4.0 78 4.5 79 4.0 80 5.0 81 5.5 82 6.0 83 7.0 84 5.0 85 6.0 86 5.5 87 2.0 88 2.5 89 1.5 90 0.5 91 1.0 92 1.0 93 0.5 94 1.0 95 2.0 96 2.0 97 3.0 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6545624561676927 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0038962050962362657 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12833.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.4623236967194 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80413663428392 99.26751344190758 2 0.15669069257286117 0.3116964076989013 3 0.007834534628643058 0.023377230577417595 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.007834534628643058 0.05454687134730772 8 0.0 0.0 9 0.0 0.0 >10 0.023503603885929175 0.3428660484687914 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC 17 0.13247097327203305 No Hit CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG 14 0.10909374269461544 No Hit CTTTACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGG 13 0.10130133250214292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0077924101924725315 0.0 10 0.0 0.0 0.0 0.0077924101924725315 0.0 11 0.0 0.0 0.0 0.0077924101924725315 0.0 12 0.0 0.0 0.0 0.0077924101924725315 0.0 13 0.0 0.0 0.0 0.0077924101924725315 0.0 14 0.0 0.0 0.0 0.0077924101924725315 0.0 15 0.0 0.0 0.0 0.0077924101924725315 0.0 16 0.0 0.0 0.0 0.0077924101924725315 0.0 17 0.0 0.0 0.0 0.015584820384945063 0.0 18 0.0 0.0 0.0 0.03896205096236266 0.0 19 0.0 0.0 0.0 0.03896205096236266 0.0 20 0.0 0.0 0.0 0.04675446115483519 0.0 21 0.0 0.0 0.0 0.04675446115483519 0.0 22 0.0 0.0 0.0 0.04675446115483519 0.0 23 0.0 0.0 0.0 0.04675446115483519 0.0 24 0.0 0.0 0.0 0.04675446115483519 0.0 25 0.0 0.0 0.0 0.04675446115483519 0.0 26 0.0 0.0 0.0 0.04675446115483519 0.0 27 0.0 0.0 0.0 0.04675446115483519 0.0 28 0.0 0.0 0.0 0.04675446115483519 0.0 29 0.0 0.0 0.0 0.04675446115483519 0.0 30 0.0 0.0 0.0 0.04675446115483519 0.0 31 0.0 0.0 0.0 0.04675446115483519 0.0 32 0.0 0.0 0.0 0.04675446115483519 0.0 33 0.0 0.0 0.0 0.04675446115483519 0.0 34 0.0 0.0 0.0 0.07792410192472532 0.0 35 0.0 0.0 0.0 0.09350892230967038 0.0 36 0.0 0.0 0.0 0.14026338346450556 0.0 37 0.0 0.0 0.0 0.18701784461934076 0.0 38 0.0 0.0 0.0 0.22597989558170342 0.0 39 0.0 0.0 0.0 0.23377230577417596 0.0 40 0.0 0.0 0.0 0.25714953635159354 0.0 41 0.0 0.0 0.0 0.2961115873139562 0.0 42 0.0 0.0 0.0 0.32728122808384635 0.0 43 0.0 0.0 0.0 0.35845086885373645 0.0 44 0.0 0.0 0.0 0.3896205096236266 0.0 45 0.0 0.0 0.0 0.4129977402010442 0.0 46 0.0 0.0 0.0 0.45975220135587935 0.0 47 0.0 0.0 0.0 0.5065066625107145 0.0 48 0.0 0.0 0.0 0.5220914828956597 0.0 49 0.0 0.0 0.0 0.5688459440504948 0.0 50 0.0 0.0 0.0 0.5844307644354398 0.0 51 0.0 0.0 0.0 0.6078079950128574 0.0 52 0.0 0.0 0.0 0.6078079950128574 0.0 53 0.0 0.0 0.0 0.6311852255902751 0.0 54 0.0 0.0 0.0 0.6545624561676927 0.0 55 0.0 0.0 0.0 0.6701472765526377 0.0 56 0.0 0.0 0.0 0.7091093275150004 0.0 57 0.0 0.0 0.0 0.7246941478999455 0.0 58 0.0 0.0 0.0 0.748071378477363 0.0 59 0.0 0.0 0.0 0.7636561988623081 0.0 60 0.0 0.0 0.0 0.7636561988623081 0.0 61 0.0 0.0 0.0 0.7714486090547806 0.0 62 0.0 0.0 0.0 0.7792410192472532 0.0 63 0.0 0.0 0.0 0.7792410192472532 0.0 64 0.0 0.0 0.0 0.8104106600171433 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAATG 35 1.9736612E-5 49.997208 25 GAATGTG 30 5.664616E-4 46.664062 27 CGAATGT 30 5.664616E-4 46.664062 26 AATGTGT 30 5.664616E-4 46.664062 28 CGCCGTA 30 5.664616E-4 46.664062 50 CGAACGA 40 4.3454886E-5 43.74756 22 ATGTGTG 35 0.0012101645 39.99777 29 GTGTGTA 35 0.0012101645 39.99777 31 TGTGTGT 35 0.0012101645 39.99777 30 AACGAAT 45 8.705007E-5 38.88672 24 GAACGAA 45 8.705007E-5 38.88672 23 TCTGACG 95 1.5279511E-10 36.84005 8 GTCGCCG 40 0.0023320918 34.998047 48 TCGCCGT 40 0.0023320918 34.998047 49 GGTCGCC 40 0.0023320918 34.998047 47 CTGACGC 95 6.4756023E-9 33.156044 9 GACGCTG 95 6.4756023E-9 33.156044 11 TGACGCT 95 6.4756023E-9 33.156044 10 CGCTGCC 85 9.326868E-8 32.93934 13 ACGAACG 55 2.833376E-4 31.816406 21 >>END_MODULE