FastQCFastQC Report
Wed 25 May 2016
SRR1780488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12833
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT342126.657835268448533No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT255919.94077768253721Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC8316.475492869944674No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC4503.506584586612639No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG4013.124756487181485No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT2381.8545936258084625Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT1921.496142756954726No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1351.0519753759837918No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1210.9428816332891764No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1010.7870334294397257No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT910.7091093275150004No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT840.6545624561676927No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT740.5766383542429673No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC680.5298838930881322No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT660.5142990727031871No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG630.4909218421257695No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC570.44416738097093433No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC490.38182809943115403No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT440.3428660484687914No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG420.32728122808384635No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC410.3194888178913738No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG400.3116964076989013No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT390.30390399750642877No Hit
CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT360.28052676692901113Illumina PCR Primer Index 1 (95% over 21bp)
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC330.25714953635159354No Hit
ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT330.25714953635159354Illumina PCR Primer Index 1 (95% over 24bp)
TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT300.23377230577417596No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGT290.22597989558170345No Hit
ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT280.21818748538923088TruSeq Adapter, Index 1 (95% over 22bp)
ATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTGCTT270.21039507519675835Illumina PCR Primer Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT270.21039507519675835No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG260.20260266500428584No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGT250.1948102548118133No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGT230.17922543442686822No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT220.1714330242343957No Hit
CTTATACACATCTCCGAGCACACGAGACTGGTCACAATCTCGTATGCCGT210.16364061404192318No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT210.16364061404192318No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT200.15584820384945064No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT200.15584820384945064No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTAGTATGCCGT200.15584820384945064No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCG190.1480557936569781No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGT190.1480557936569781No Hit
ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT190.1480557936569781TruSeq Adapter, Index 4 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGTCTT190.1480557936569781TruSeq Adapter, Index 4 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTC190.1480557936569781No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC190.1480557936569781No Hit
ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT180.14026338346450556Illumina PCR Primer Index 1 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCG170.13247097327203305No Hit
CTTATACACATATCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT170.13247097327203305No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCCT170.13247097327203305No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC170.13247097327203305No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAGCTCGTATGCCGT160.1246785630795605No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATTTCGTATGCCGT160.1246785630795605No Hit
ATTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT160.1246785630795605No Hit
CTTATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGT150.11688615288708798No Hit
TCTCATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG150.11688615288708798No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGACGTCTT140.10909374269461544No Hit
CTTATACACATCTACGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT140.10909374269461544No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGT140.10909374269461544No Hit
CTTATACACATCTCCGCGCCCACGAGACTGGTCACAATCTCGTATGCCGT140.10909374269461544No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCACGTATGCCGT140.10909374269461544No Hit
TCTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT130.10130133250214292No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGTCTT130.10130133250214292No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT130.10130133250214292Illumina PCR Primer Index 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACT252.683706E-670.034
AAACTCG207.633407E-570.036
TTGCAAA252.683706E-670.058
CTTTATA150.002174843370.02
CTTTACA1150.070.01
TGCAAAA252.683706E-670.059
AAATACA207.633407E-570.070
GCAAAAA252.683706E-670.060
TGCTTGC252.683706E-670.055
CTTACAC150.002174843370.01
GCTTGCA252.683706E-670.056
CTTGCAA252.683706E-670.057
CAAACTC207.633407E-570.035
TCTTTAT150.002174843370.01
TCACAAA150.002174843370.032
TTTATAC150.002174843370.03
TTTACAC1200.067.083332
CTATACA800.065.6251
CTTATAC5200.057.2115361
ACTCGTA252.3025334E-455.99999638
Produced by FastQC (version 0.11.5)