##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780488_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12833 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.719784929478685 34.0 34.0 34.0 31.0 34.0 2 33.25940933530741 34.0 34.0 34.0 32.0 34.0 3 33.43162160056105 34.0 34.0 34.0 34.0 34.0 4 33.503857243045275 34.0 34.0 34.0 34.0 34.0 5 33.55287150315593 34.0 34.0 34.0 34.0 34.0 6 37.39328294241409 38.0 38.0 38.0 37.0 38.0 7 36.92340060780799 38.0 37.0 38.0 35.0 38.0 8 37.00194810254812 38.0 37.0 38.0 36.0 38.0 9 36.71417439414011 38.0 37.0 38.0 34.0 38.0 10-11 36.41825761708096 38.0 37.0 38.0 34.0 38.0 12-13 36.05318319956363 38.0 36.5 38.0 33.0 38.0 14-15 36.04461154835191 38.0 36.0 38.0 32.5 38.0 16-17 35.91743941401076 38.0 36.0 38.0 32.0 38.0 18-19 36.05766383542429 38.0 36.5 38.0 32.5 38.0 20-21 35.978687758123584 38.0 36.0 38.0 32.0 38.0 22-23 35.97510324943505 38.0 36.5 38.0 32.5 38.0 24-25 34.59935322995402 37.0 34.0 38.0 28.0 38.0 26-27 34.83826852645523 37.0 34.0 38.0 29.0 38.0 28-29 35.03611782124211 37.0 35.5 38.0 27.0 38.0 30-31 34.59179459206733 37.0 34.5 38.0 26.5 38.0 32-33 35.374737006156 37.0 36.0 38.0 31.0 38.0 34-35 34.27121483674901 37.0 35.0 38.0 25.0 38.0 36-37 33.74296734980129 37.0 34.0 38.0 24.0 38.0 38-39 31.297436297046676 36.0 27.0 37.0 11.0 38.0 40-41 33.256720953791 36.5 32.5 37.5 23.5 38.0 42-43 34.87711369126471 37.0 34.5 38.0 29.0 38.0 44-45 35.26466921218733 37.0 36.0 38.0 30.5 38.0 46-47 35.23381126782514 37.0 36.0 38.0 31.0 38.0 48-49 34.74717525130523 37.0 35.0 38.0 28.0 38.0 50-51 34.634535961973036 37.0 34.0 38.0 26.0 38.0 52-53 34.31976155224811 37.0 34.0 38.0 25.5 38.0 54-55 34.729447518117354 37.0 35.0 38.0 26.5 38.0 56-57 34.56876801994857 37.0 34.0 38.0 27.0 38.0 58-59 34.24101924725318 37.0 34.0 38.0 25.0 38.0 60-61 34.22348632432011 37.0 34.0 38.0 25.5 38.0 62-63 34.96984337255513 37.0 35.0 38.0 27.0 38.0 64-65 34.90707550845477 37.0 35.0 38.0 29.0 38.0 66-67 32.597755785864564 36.5 31.0 38.0 21.0 38.0 68-69 28.12635393127094 32.5 21.5 37.0 11.0 38.0 70-71 23.835930803397492 25.0 11.0 33.5 10.5 37.0 72-73 19.865697810332737 21.0 11.0 25.0 10.5 34.0 74-75 19.337177589028286 19.0 11.0 25.0 11.0 34.0 76 19.678173459050885 20.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20273117980067212 1101 2 0.48825962978126114 1101 3 0.3510528735334475 1101 4 0.47012809629216434 1101 5 0.3501582794652336 1101 6 -2.0931582153886197 1101 7 0.3549073660587112 1101 8 0.6479796997459886 1101 9 1.1206919814928682 1101 10-11 -1.4096803249054233 1101 12-13 -0.018514642005825976 1101 14-15 0.09580288615844523 1101 16-17 -0.4404110914218009 1101 18-19 -0.3464000798487987 1101 20-21 1.1473391557913573 1101 22-23 0.806876419047839 1101 24-25 -2.1208577809234797 1101 26-27 -0.2823190953270256 1101 28-29 1.0482319450699649 1101 30-31 2.1283426624171966 1101 32-33 1.0092711878740062 1101 34-35 0.9105756891998809 1101 36-37 -0.19397258093447078 1101 38-39 0.7066973837023696 1101 40-41 2.873137386247116 1101 42-43 1.128615237617005 1101 44-45 1.1689315974261874 1101 46-47 2.1503529868424707 1101 48-49 -1.5460575419813125 1101 50-51 -1.7609183398829558 1101 52-53 -3.0683515420716816 1101 54-55 -0.40135277066880093 1101 56-57 -0.8960313739359975 1101 58-59 -1.7911386948423598 1101 60-61 -3.100548990629278 1101 62-63 -2.1996071127077244 1101 64-65 -2.270218662281998 1101 66-67 -2.998902295109872 1101 68-69 -1.370954526185983 1101 70-71 -3.2868202157533695 1101 72-73 -3.1286933784706257 1101 74-75 -1.3612726730054483 1101 76 0.6202464914224066 1102 1 -0.4535188201993279 1102 2 -0.04299037021873886 1102 3 0.4760528735334475 1102 4 0.43887809629216434 1102 5 0.4751582794652336 1102 6 0.4693417846113803 1102 7 -0.5825926339412888 1102 8 -0.13327030025401143 1102 9 0.8706919814928682 1102 10-11 0.6996946750945767 1102 12-13 1.247110357994174 1102 14-15 -0.09169711384155477 1102 16-17 -0.0966610914218009 1102 18-19 0.10672492015120127 1102 20-21 0.31921415579135726 1102 22-23 -0.13062358095216098 1102 24-25 -7.43335778092348 1102 26-27 -1.4698190953270256 1102 28-29 1.2201069450699649 1102 30-31 2.5502176624171966 1102 32-33 -0.13135381212599384 1102 34-35 1.1605756891998809 1102 36-37 -2.709597580934471 1102 38-39 -0.6526776162976304 1102 40-41 1.060637386247116 1102 42-43 0.1286152376170051 1102 44-45 0.9189315974261874 1102 46-47 1.4472279868424707 1102 48-49 -4.1398075419813125 1102 50-51 -0.5109183398829558 1102 52-53 1.1660234579283184 1102 54-55 1.692397229331199 1102 56-57 2.2445936260640025 1102 58-59 -0.40051369484235977 1102 60-61 -6.709923990629278 1102 62-63 -1.7308571127077244 1102 64-65 0.19853133771800202 1102 66-67 2.079222704890128 1102 68-69 -2.370954526185983 1102 70-71 -1.1461952157533695 1102 72-73 -0.7849433784706257 1102 74-75 0.6387273269945517 1102 76 -2.0360035085775934 1103 1 0.6105055700445732 1103 2 0.7695096297812611 1103 3 0.4684309223139351 1103 4 0.15991468165801592 1103 5 0.5689082794652336 1103 6 -0.39879845929105784 1103 7 0.9448463904489586 1103 8 0.3819736021850133 1103 9 -0.22153362826323075 1103 10-11 0.6718745531433541 1103 12-13 -0.8340634224936281 1103 14-15 -0.08102638213423319 1103 16-17 0.10341512809039699 1103 18-19 0.6177767494194981 1103 20-21 -0.036730966159865375 1103 22-23 0.30649532148686376 1103 24-25 -3.912778512630794 1103 26-27 -1.1683709245953153 1103 28-29 -0.5782924451739362 1103 30-31 1.8238457111976913 1103 32-33 1.369789480556932 1103 34-35 -0.037213944946458355 1103 36-37 -1.8124939223978842 1103 38-39 0.7112705544340763 1103 40-41 0.4988995813690664 1103 42-43 0.17701462786090616 1103 44-45 1.5862334266944842 1103 46-47 1.4899109136717428 1103 48-49 -2.6771551029569167 1103 50-51 -0.1271530959805105 1103 52-53 0.6084777262209968 1103 54-55 -1.371627160912702 1103 56-57 -0.562952105643312 1103 58-59 -0.1970075972813845 1103 60-61 -5.117698380873179 1103 62-63 -1.4686619907565017 1103 64-65 -0.818618052525899 1103 66-67 -2.884954124378165 1103 68-69 -1.2314728188689088 1103 70-71 -2.2780549718509313 1103 72-73 -1.0600958174950144 1103 74-75 0.5788950099213821 1103 76 -0.23493643540686193 1104 1 0.4571954655149568 1104 2 0.05522391549554584 1104 3 0.4921243021048767 1104 4 -0.09862190370783708 1104 5 -0.9739488633919109 1104 6 0.05416321318281092 1104 7 0.5566930803444237 1104 8 0.6488725568888469 1104 9 0.9108705529214376 1104 10-11 -2.0587874677625706 1104 12-13 -0.3417289277201121 1104 14-15 0.8886600290155897 1104 16-17 0.8671781942924852 1104 18-19 0.9205642058654888 1104 20-21 -0.06739298706578722 1104 22-23 0.13053713333355432 1104 24-25 -0.05389349520919495 1104 26-27 1.0859844761015438 1104 28-29 1.6464462307842567 1104 30-31 1.561824805274341 1104 32-33 1.069092616445431 1104 34-35 -0.059513596514406686 1104 36-37 2.135938133351246 1104 38-39 1.5941973837023724 1104 40-41 2.1508159576756825 1104 42-43 0.4094188090455759 1104 44-45 1.831878025997618 1104 46-47 1.7115137011281902 1104 48-49 0.593228172304407 1104 50-51 0.20559951725990544 1104 52-53 1.638791315071174 1104 54-55 -0.34688848495451197 1104 56-57 0.967361483206858 1104 58-59 -0.9781922662709306 1104 60-61 0.9356117236564359 1104 62-63 0.06200003014942013 1104 64-65 1.2632634805751408 1104 66-67 -0.61631300939559 1104 68-69 1.3406526166711608 1104 70-71 1.797554784246632 1104 72-73 -1.2304790927563367 1104 74-75 -1.6036833872911629 1104 76 -0.799396365720451 1105 1 1.0495299602884742 1105 2 0.4524364590495509 1105 3 0.6635528735334475 1105 4 0.1111341938531396 1105 5 0.4957375477579191 1105 6 0.4060795894894298 1105 7 0.8472854148391988 1105 8 0.28441262657525357 1105 9 0.9735883229562816 1105 10-11 1.3060208946067746 1105 12-13 -0.10235610542046203 1105 14-15 0.27263215445112365 1105 16-17 0.37170781101722383 1105 18-19 1.1665572372243744 1105 20-21 0.25595196066940673 1105 22-23 -0.16911443461069808 1105 24-25 0.6969775849301811 1105 26-27 1.6487022461363878 1105 28-29 1.238780725557767 1105 30-31 2.1165286380269492 1105 32-33 1.808813870800833 1105 34-35 -0.9274578473854831 1105 36-37 1.6875060776021087 1105 38-39 0.6502949446779773 1105 40-41 -0.4401248088748346 1105 42-43 0.21359999371456695 1105 44-45 1.2447700120603358 1105 46-47 2.0021060356229583 1105 48-49 -0.26252095661546093 1105 50-51 1.4826030015804577 1105 52-53 1.2304289457331947 1105 54-55 2.2991045464043722 1105 56-57 1.9248527724054654 1105 58-59 1.644455817352764 1105 60-61 1.7237650337609693 1105 62-63 -0.724759551732113 1105 64-65 1.705772191376532 1105 66-67 2.0784605097681776 1105 68-69 -0.7192776969176933 1105 70-71 0.9658474671734609 1105 72-73 0.3911236947001058 1105 74-75 0.7862120830921135 1105 76 -1.0398144841873496 1106 1 0.2597164739183171 1106 2 0.5048037474283191 1106 3 0.6635528735334475 1106 4 -0.08685719782548063 1106 5 0.36302592652405963 1106 6 0.4785329610819673 1106 7 -0.16347498688246276 1106 8 0.4329061703342205 1106 9 -0.6899697832130158 1106 10-11 0.9928932045063448 1106 12-13 1.720455946229471 1106 14-15 0.47815582733491624 1106 16-17 -0.06265373848062694 1106 18-19 1.0819087436806143 1106 20-21 0.5636994499090022 1106 22-23 0.636839654341955 1106 24-25 0.028039277900049342 1106 26-27 1.9557323752612064 1106 28-29 0.8460260627170229 1106 30-31 1.4601441330054286 1106 32-33 0.4816976584622381 1106 34-35 0.9556124539057649 1106 36-37 2.3510641837714132 1106 38-39 0.33537385429060507 1106 40-41 2.205857974482406 1106 42-43 0.9879902376170051 1106 44-45 1.5650713033085353 1106 46-47 1.5060515162542387 1106 48-49 2.1083542227245715 1106 50-51 0.34845666011704424 1106 52-53 1.3434131638106663 1106 54-55 0.5968089940370831 1106 56-57 1.3484539201816474 1106 58-59 1.5167657169223503 1106 60-61 2.336031891723664 1106 62-63 0.9014958284687467 1106 64-65 -0.747240721105527 1106 66-67 1.6803256460665992 1106 68-69 3.627207238519901 1106 70-71 1.021084196011337 1106 72-73 0.47792426858820036 1106 74-75 -1.9835153200642708 1106 76 -0.7069593909305354 1107 1 0.5135864429585695 1107 2 0.21687805083389122 1107 3 0.6372370840597625 1107 4 0.42243072787110947 1107 5 0.41101354262313095 1107 6 0.5466444161903254 1107 7 0.8845126292166086 1107 8 -0.16616503709611408 1107 9 -1.055294860612392 1107 10-11 0.5656486224630015 1107 12-13 -0.24548832621635341 1107 14-15 0.3589607808952877 1107 16-17 0.5735691717360893 1107 18-19 1.1322183412038314 1107 20-21 0.7254641557913573 1107 22-23 0.08730405062678415 1107 24-25 0.7788132717080956 1107 26-27 -0.24860199006386807 1107 28-29 1.6238898398068073 1107 30-31 1.5979150308382515 1107 32-33 1.7115738194529513 1107 34-35 -0.12643088974748906 1107 36-37 1.4663892611707894 1107 38-39 2.0915658047549996 1107 40-41 1.529387386247116 1107 42-43 0.8695691849854299 1107 44-45 0.5612013342682971 1107 46-47 1.1840700921056282 1107 48-49 1.815784563281845 1107 50-51 2.0984566601170442 1107 52-53 2.339543194770428 1107 54-55 1.672660387225939 1107 56-57 1.2865344155376874 1107 58-59 1.1800784104207978 1107 60-61 2.3607996935812494 1107 62-63 1.808616571502803 1107 64-65 1.6598800219285295 1107 66-67 -0.5441325582677692 1107 68-69 1.7293744211824382 1107 70-71 -2.3813925841744243 1107 72-73 -0.5135617995232558 1107 74-75 -0.17623977826860582 1107 76 -1.8106745612091721 1108 1 0.8894298977493875 1108 2 0.46181732208895454 1108 3 -0.0031137931332168023 1108 4 0.42445501936908414 1108 5 -0.1490404384834818 1108 6 -0.2718441128245175 1108 7 0.042407366058711204 1108 8 -0.23903953102324493 1108 9 0.1855958276467149 1108 10-11 0.6071465981714965 1108 12-13 1.5952674092762251 1108 14-15 1.0637516041071606 1108 16-17 -0.3775104503961657 1108 18-19 0.8451063304076101 1108 20-21 1.5652077455349485 1108 22-23 -0.10938960659318298 1108 24-25 1.468084526768827 1108 26-27 1.1684020585191348 1108 28-29 -1.049524465186444 1108 30-31 0.5317881752377076 1108 32-33 1.3482134955663128 1108 34-35 0.37491863791782976 1108 36-37 2.4370370344501424 1108 38-39 2.8813768708818586 1108 40-41 0.4845155913753203 1108 42-43 -0.5055995059727394 1108 44-45 1.723819417939005 1108 46-47 1.3126126022270839 1108 48-49 2.1302245093007386 1108 50-51 1.8740976857580662 1108 52-53 -0.010659234379374993 1108 54-55 -0.7402950783611075 1108 56-57 1.7538083696537399 1108 58-59 1.3214414333627644 1108 60-61 2.6161978042425176 1108 62-63 1.8268351949845822 1108 64-65 1.9152781325897976 1108 66-67 2.0415624484798727 1108 68-69 3.7901031661217104 1108 70-71 1.8129393996312473 1108 72-73 1.6581655958883488 1108 74-75 2.284961301353526 1108 76 -0.8653304316545167 1109 1 -2.403518820199327 1109 2 -0.0554903702187417 1109 3 0.5385528735334475 1109 4 0.47637809629216576 1109 5 0.06890827946523359 1109 6 -0.0994082153886211 1109 7 -0.9075926339412916 1109 8 -0.20827030025401427 1109 9 0.9894419814928668 1109 10-11 -0.09093032490542186 1109 12-13 0.18148535799417687 1109 14-15 0.7333028861584481 1109 16-17 0.6564639085781963 1109 18-19 0.9348499201512013 1109 20-21 0.9754641557913573 1109 22-23 0.09125141904783618 1109 24-25 0.34789221907652035 1109 26-27 0.3895559046729744 1109 28-29 -1.5392680549300408 1109 30-31 -0.5810323375827977 1109 32-33 0.865521187874009 1109 34-35 -0.9487993108001191 1109 36-37 2.221652419065535 1109 38-39 1.7191973837023724 1109 40-41 1.454387386247113 1109 42-43 0.7379902376170051 1109 44-45 0.8408065974261874 1109 46-47 1.109727986842472 1109 48-49 0.40394245801869033 1109 50-51 -0.5765433398829529 1109 52-53 -0.543351542071683 1109 54-55 0.06739722933119907 1109 56-57 -0.37728137393600036 1109 58-59 0.11823630515763739 1109 60-61 1.1838260093707191 1109 62-63 0.39414288729227565 1109 64-65 1.7079063377180006 1109 66-67 1.2854727048901253 1109 68-69 0.7102954738140141 1109 70-71 0.38505478424663053 1109 72-73 1.2963066215293715 1109 74-75 -0.2643976730054476 1109 76 -0.9922535085775941 1110 1 -2.4396299313104386 1110 2 0.24173185200348257 1110 3 -0.7253360153554382 1110 4 -0.05417745926339279 1110 5 0.4022416127985693 1110 6 0.12559178461138032 1110 7 0.7646295882809326 1110 8 0.6306185886348743 1110 9 1.033886425937311 1110 10-11 -0.9270414360165375 1110 12-13 0.7662075802163955 1110 14-15 1.1235806639362238 1110 16-17 0.6897972419115348 1110 18-19 -0.41237230207102726 1110 20-21 0.9199086002358001 1110 22-23 0.16625141904783902 1110 24-25 -1.7840522253679225 1110 26-27 1.4312225713396458 1110 28-29 0.6940652784032935 1110 30-31 -0.38381011536058196 1110 32-33 0.6655211878740062 1110 34-35 1.5789784669776523 1110 36-37 2.766096863509972 1110 38-39 2.0330862725912553 1110 40-41 -2.0261681693084412 1110 42-43 -0.2620097623829949 1110 44-45 0.7783065974261874 1110 46-47 0.9194502090646921 1110 48-49 2.162275791352016 1110 50-51 0.001234437894822804 1110 52-53 0.7788706801505398 1110 54-55 -0.7937138817799152 1110 56-57 2.024107514952888 1110 58-59 1.1099029718243045 1110 60-61 0.5157704538151648 1110 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-1.8462603289486665 2116 66-67 -1.535360628443204 2116 68-69 -0.31887119285265086 2116 70-71 0.8308881175799634 2116 72-73 0.7171399548627058 2116 74-75 2.477268993661216 2116 76 -0.10892017524426123 2117 1 0.3650295668974479 2117 2 0.38241285558770954 2117 3 0.5667786799850631 2117 4 0.3723458382276448 2117 5 0.5689082794652336 2117 6 0.27075307493396394 2117 7 0.3972460757361276 2117 8 0.7659232481330847 2117 9 -1.3379773733458435 2117 10-11 -0.6546400023247827 2117 12-13 -1.5205307710380822 2117 14-15 -0.3638745331963946 2117 16-17 -0.7443425430346977 2117 18-19 -1.887730725010087 2117 20-21 -1.7987293925957388 2117 22-23 -1.0353614841779688 2117 24-25 -1.1944464906009031 2117 26-27 -2.9370569985528334 2117 28-29 -2.0102357968655227 2117 30-31 -1.7874839504860276 2117 32-33 -4.846575586319542 2117 34-35 0.7649103666192403 2117 36-37 -1.968670161579631 2117 38-39 -3.2348348743621465 2117 40-41 -2.736741646010948 2117 42-43 -0.003945246253962864 2117 44-45 -0.45153211225123613 2117 46-47 -2.980191367996241 2117 48-49 -1.3726704452071203 2117 50-51 -1.0547691463345714 2117 52-53 -0.7860934775555535 2117 54-55 -0.6422801900236408 2117 56-57 -0.844620083613421 2117 58-59 -1.5164411141971996 2117 60-61 -1.0980288293389577 2117 62-63 0.2772074034213077 2117 64-65 -1.9924968880884464 2117 66-67 -0.6858982628518078 2117 68-69 -0.12801097779888693 2117 70-71 -0.6572839254307894 2117 72-73 -0.5581288623415936 2117 74-75 -0.7055267052635124 2117 76 0.7029077817449867 2118 1 0.07470698625228778 2118 2 0.6404773717167416 2118 3 -3.2074148684020365 2118 4 0.11428132209861275 2118 5 0.5689082794652336 2118 6 0.14172081686944438 2118 7 0.719826720897423 2118 8 0.9594716352298605 2118 9 -2.241203179797452 2118 10-11 -0.6385109700667186 2118 12-13 -1.730208190392922 2118 14-15 0.7167706280939328 2118 16-17 -5.228213510776641 2118 18-19 -1.4683758863004144 2118 20-21 -1.0245358442086427 2118 22-23 0.7065739996929992 2118 24-25 0.24103738036684064 2118 26-27 -2.4047989340367053 2118 28-29 -0.5747519258977789 2118 30-31 -1.2229678214537714 2118 32-33 -0.10464010244857036 2118 34-35 0.6520071408127848 2118 36-37 -7.017057258353823 2118 38-39 -0.08967358403956638 2118 40-41 -0.6399674524625638 2118 42-43 1.205732173100877 2118 44-45 -2.435403079993165 2118 46-47 -0.9479333034801058 2118 48-49 0.6434585870509437 2118 50-51 -0.34509172697972446 2118 52-53 -1.2860934775555535 2118 54-55 -3.222925351313961 2118 56-57 -1.4897813739359975 2118 58-59 -1.9196669206488082 2118 60-61 -1.3883514099841179 2118 62-63 -0.9647280804496603 2118 64-65 0.36234182158897 2118 66-67 -0.1375111660776156 2118 68-69 -2.982849687476307 2118 70-71 -0.1895419899469175 2118 72-73 1.7160646860455024 2118 74-75 2.4235055528010037 2118 76 -0.4261244763195293 2119 1 -0.2900572817377878 2119 2 -2.3458749856033556 2119 3 -0.8364471264665525 2119 4 -1.8063142114001423 2119 5 -0.5849378743809197 2119 6 -0.18210052308092628 2119 7 -1.8422080185566756 2119 8 -1.0467318387155515 2119 9 -0.3144041723532851 2119 10-11 -1.3543918633669634 2119 12-13 -1.0906300266212128 2119 14-15 -0.6541971138415548 2119 16-17 -0.2685360914218009 2119 18-19 -0.8728423875411053 2119 20-21 -2.2553050749778762 2119 22-23 -1.2760562732598544 2119 24-25 0.44885375753805334 2119 26-27 -0.9662133260962591 2119 28-29 -0.25465267031464833 2119 30-31 -0.057955414505883596 2119 32-33 0.8097519571047798 2119 34-35 0.44543145843064735 2119 36-37 -0.5245014270883175 2119 38-39 -2.5673410778360903 2119 40-41 -3.8359972291374973 2119 42-43 -3.223548223921455 2119 44-45 -1.3851549410353456 2119 46-47 -0.32200278238830293 2119 48-49 -1.6325960035197724 2119 50-51 0.30999512165551124 2119 52-53 -1.6837361574562948 2119 54-55 -2.0287566168226405 2119 56-57 -4.259012143166768 2119 58-59 -1.8452252333038928 2119 60-61 -0.2748278367831247 2119 62-63 -1.827010958861571 2119 64-65 -0.14882443151276448 2119 66-67 0.7595111664285952 2119 68-69 0.3862570122755571 2119 70-71 0.10140093809278383 2119 72-73 -2.0277318400090856 2119 74-75 -1.285551519159295 2119 76 -0.4422535085775934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 2.0 22 3.0 23 5.0 24 26.0 25 40.0 26 104.0 27 154.0 28 309.0 29 530.0 30 774.0 31 1088.0 32 1516.0 33 2120.0 34 2936.0 35 2577.0 36 594.0 37 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.7792410192472532 29.174783760617157 12.226291591989401 57.81968362814619 2 0.4675446115483519 3.66243279046209 86.36328216317307 9.50674043481649 3 0.5844307644354398 33.99828566975766 62.6665627678641 2.7507207979428037 4 0.366243279046209 45.68690095846645 24.694147899945452 29.252707862541882 5 0.366243279046209 51.22730460531443 44.13621133016442 4.270240785474948 6 0.5454687134730772 47.689550377931894 8.595028442297203 43.16995246629783 7 1.1376918881009896 51.32860593781656 4.714408166445882 42.81929400763656 8 0.4675446115483519 47.05057274214915 28.74620120003117 23.735681446271332 9 1.0831450167536818 23.626587703576714 3.639055559884672 71.65121171978492 10-11 0.7597599937660718 26.447440193251776 42.5543520610925 30.23844775188966 12-13 14.684797007714486 3.5650276630561835 33.42943972570716 48.32073560352217 14-15 23.35385334684018 16.15756253409179 6.904075430530663 53.58450868853737 16-17 32.87617860204161 27.86176264318554 0.8298916854983246 38.43216706927453 18-19 26.291591989402324 21.22262915919894 0.5961193797241486 51.88965947167459 20-21 16.897841502376686 10.41455622223954 0.9701550689628302 71.71744720642094 22-23 23.587625652614353 39.67895270007013 0.7714486090547806 35.96197303826074 24-25 32.841112756175484 44.553105275461704 1.3286059378165667 21.27717603054625 26-27 40.19325177277332 33.49567521234318 15.986129509857399 10.324943505026104 28-29 27.02797475259098 7.675524039585444 40.90625730538456 24.390243902439025 30-31 50.420790150393515 14.832852801371466 16.040676381204708 18.705680667030315 32-33 9.241798488272423 23.84087898386971 24.565573131769654 42.35174939608821 34-35 1.1922387594482973 48.25839632198239 16.426400685732098 34.12296423283722 36-37 1.1805501441595887 53.26501987064599 23.02657211875633 22.52785786643809 38-39 14.59518429050105 11.571729135821709 31.528091638743867 42.304994934933376 40-41 9.861295098573988 15.39001013013325 41.369905711836665 33.37878905945609 42-43 37.5477285124289 9.717135510013247 45.04402711758747 7.691108859970389 44-45 11.875633133328138 24.012312008104107 34.064521156393674 30.047533702174086 46-47 39.31660562612016 2.992285513909452 21.109639211408087 36.5814696485623 48-49 29.35790540014026 1.1298994779085172 23.661653549442843 45.85054157250838 50-51 7.005376763032806 1.2857476817579676 48.4103483207356 43.298527234473625 52-53 14.353619574534404 1.4299072703187097 67.99267513441907 16.223798020727813 54-55 9.487259409335309 1.4182186550300009 49.540247798644124 39.55427413699057 56-57 37.571105743006314 1.8584898309046989 30.889114002961115 29.68129042312787 58-59 11.489908828800747 30.643653081898233 50.393516714719865 7.4729213745811585 60-61 23.700615600405207 65.09389854281929 9.20283643731006 2.0026494194654405 62-63 2.579287773708408 94.86480168316061 0.8805423517493961 1.6753681913815943 64-65 0.5104028676069509 96.91030935868464 0.6545624561676927 1.9247253175407153 66-67 0.44027117587469805 94.23751266266656 0.9272968129042313 4.394919348554508 68-69 0.997428504636484 84.99571417439414 2.731239772461622 11.275617548507753 70-71 2.2169406997584353 75.42663445803787 5.645601184446349 16.710823657757345 72-73 3.440349099976623 62.77955271565495 9.093742694615445 24.68635548975298 74-75 4.250759759993766 59.841814073092806 9.210628847502532 26.69679731941089 76 3.490999766227694 62.440582872282405 7.730070910932751 26.33834645055716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.5 25 2.0 26 2.0 27 1.5 28 1.0 29 1.0 30 2.5 31 3.0 32 4.0 33 6.0 34 4.0 35 6.0 36 13.5 37 17.0 38 43.0 39 259.5 40 1238.5 41 2580.5 42 3134.0 43 3090.0 44 2510.5 45 1522.5 46 1070.0 47 789.5 48 367.5 49 174.0 50 122.0 51 90.5 52 47.0 53 26.0 54 17.0 55 13.5 56 7.5 57 6.5 58 8.0 59 4.5 60 4.0 61 6.0 62 5.0 63 3.0 64 2.0 65 1.5 66 1.5 67 3.0 68 1.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12833.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.592612795137537 #Duplication Level Percentage of deduplicated Percentage of total 1 79.91004497751123 12.460063897763577 2 7.496251874062969 2.3377230577417594 3 3.648175912043978 1.7065378321514846 4 2.0989505247376314 1.3091249123353854 5 1.3493253373313343 1.0519753759837918 6 0.5497251374312844 0.5142990727031871 7 0.6496751624187905 0.7091093275150004 8 0.24987506246876562 0.3116964076989013 9 0.14992503748125938 0.21039507519675835 >10 3.0484757621189407 9.631418997896048 >50 0.3498250874562719 3.919582326813684 >100 0.3498250874562719 12.763967895270007 >500 0.04997501249375312 6.475492869944674 >1k 0.09995002498750624 46.59861295098574 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 3421 26.657835268448533 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 2559 19.94077768253721 Illumina PCR Primer Index 1 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 831 6.475492869944674 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 450 3.506584586612639 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 401 3.124756487181485 No Hit TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 238 1.8545936258084625 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT 192 1.496142756954726 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 135 1.0519753759837918 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 121 0.9428816332891764 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 101 0.7870334294397257 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT 91 0.7091093275150004 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT 84 0.6545624561676927 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT 74 0.5766383542429673 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 68 0.5298838930881322 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT 66 0.5142990727031871 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 63 0.4909218421257695 No Hit TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 57 0.44416738097093433 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 49 0.38182809943115403 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT 44 0.3428660484687914 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 42 0.32728122808384635 No Hit CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 41 0.3194888178913738 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 40 0.3116964076989013 No Hit CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT 39 0.30390399750642877 No Hit CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 36 0.28052676692901113 Illumina PCR Primer Index 1 (95% over 21bp) CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 33 0.25714953635159354 No Hit ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT 33 0.25714953635159354 Illumina PCR Primer Index 1 (95% over 24bp) TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 30 0.23377230577417596 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGT 29 0.22597989558170345 No Hit ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT 28 0.21818748538923088 TruSeq Adapter, Index 1 (95% over 22bp) ATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTGCTT 27 0.21039507519675835 Illumina PCR Primer Index 1 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT 27 0.21039507519675835 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG 26 0.20260266500428584 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGT 25 0.1948102548118133 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGT 23 0.17922543442686822 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT 22 0.1714330242343957 No Hit CTTATACACATCTCCGAGCACACGAGACTGGTCACAATCTCGTATGCCGT 21 0.16364061404192318 No Hit CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT 21 0.16364061404192318 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT 20 0.15584820384945064 No Hit TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 20 0.15584820384945064 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTAGTATGCCGT 20 0.15584820384945064 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCG 19 0.1480557936569781 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGT 19 0.1480557936569781 No Hit ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT 19 0.1480557936569781 TruSeq Adapter, Index 4 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGTCTT 19 0.1480557936569781 TruSeq Adapter, Index 4 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTC 19 0.1480557936569781 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC 19 0.1480557936569781 No Hit ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT 18 0.14026338346450556 Illumina PCR Primer Index 1 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCG 17 0.13247097327203305 No Hit CTTATACACATATCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 17 0.13247097327203305 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCCT 17 0.13247097327203305 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC 17 0.13247097327203305 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAGCTCGTATGCCGT 16 0.1246785630795605 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATTTCGTATGCCGT 16 0.1246785630795605 No Hit ATTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 16 0.1246785630795605 No Hit CTTATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGT 15 0.11688615288708798 No Hit TCTCATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 15 0.11688615288708798 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGACGTCTT 14 0.10909374269461544 No Hit CTTATACACATCTACGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 14 0.10909374269461544 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGT 14 0.10909374269461544 No Hit CTTATACACATCTCCGCGCCCACGAGACTGGTCACAATCTCGTATGCCGT 14 0.10909374269461544 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCACGTATGCCGT 14 0.10909374269461544 No Hit TCTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 13 0.10130133250214292 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGTCTT 13 0.10130133250214292 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT 13 0.10130133250214292 Illumina PCR Primer Index 1 (95% over 22bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0077924101924725315 0.0 18 0.0 0.0 0.0 0.03896205096236266 0.0 19 0.0 0.0 0.0 0.03896205096236266 0.0 20 0.0 0.0 0.0 0.04675446115483519 0.0 21 0.0 0.0 0.0 0.04675446115483519 0.0 22 0.0 0.0 0.0 0.04675446115483519 0.0 23 0.0 0.0 0.0 0.04675446115483519 0.0 24 0.0 0.0 0.0 0.04675446115483519 0.0 25 0.0 0.0 0.0 0.05454687134730772 0.0 26 0.0 0.0 0.0 0.05454687134730772 0.0 27 0.0 0.0 0.0 0.05454687134730772 0.0 28 0.0 0.0 0.0 0.05454687134730772 0.0 29 0.0 0.0 0.0 0.05454687134730772 0.0 30 0.0 0.0 0.0 0.05454687134730772 0.0 31 0.0 0.0 0.0 0.05454687134730772 0.0 32 0.0 0.0 0.0 0.06233928153978025 0.0 33 0.0 0.0 0.0 0.06233928153978025 0.0 34 0.0 0.0 0.0 0.07013169173225278 0.0 35 0.0 0.0 0.0 0.07792410192472532 0.0 36 0.0 0.0 0.0 0.1246785630795605 0.0 37 0.0 0.0 0.0 0.14026338346450556 0.0 38 0.0 0.0 0.0 0.15584820384945064 0.0 39 0.0 0.0 0.0 0.15584820384945064 0.0 40 0.0 0.0 0.0 0.16364061404192318 0.0 41 0.0 0.0 0.0 0.16364061404192318 0.0 42 0.0 0.0 0.0 0.1948102548118133 0.0 43 0.0 0.0 0.0 0.22597989558170342 0.0 44 0.0 0.0 0.0 0.25714953635159354 0.0 45 0.0 0.0 0.0 0.2727343567365386 0.0 46 0.0 0.0 0.0 0.28052676692901113 0.0 47 0.0 0.0 0.0 0.28052676692901113 0.0 48 0.0 0.0 0.0 0.2883191771214837 0.0 49 0.0 0.0 0.0 0.3039039975064287 0.0 50 0.0 0.0 0.0 0.3116964076989013 0.0 51 0.0 0.0 0.0 0.32728122808384635 0.0 52 0.0 0.0 0.0 0.33507363827631886 0.0 53 0.0 0.0 0.0 0.33507363827631886 0.0 54 0.0 0.0 0.0 0.35065845866126394 0.0 55 0.0 0.0 0.0 0.35845086885373645 0.0 56 0.0 0.0 0.0 0.38182809943115403 0.0 57 0.0 0.0 0.0 0.3974129198160991 0.0 58 0.0 0.0 0.0 0.3974129198160991 0.0 59 0.0 0.0 0.0 0.3974129198160991 0.0 60 0.0 0.0 0.0 0.4052053300085717 0.0 61 0.0 0.0 0.0 0.4129977402010442 0.0 62 0.0 0.0 0.0 0.42858256058598926 0.0 63 0.0 0.0 0.0 0.43637497077846177 0.0 64 0.0 0.0 0.0 0.44416738097093433 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAACT 25 2.683706E-6 70.0 34 AAACTCG 20 7.633407E-5 70.0 36 TTGCAAA 25 2.683706E-6 70.0 58 CTTTATA 15 0.0021748433 70.0 2 CTTTACA 115 0.0 70.0 1 TGCAAAA 25 2.683706E-6 70.0 59 AAATACA 20 7.633407E-5 70.0 70 GCAAAAA 25 2.683706E-6 70.0 60 TGCTTGC 25 2.683706E-6 70.0 55 CTTACAC 15 0.0021748433 70.0 1 GCTTGCA 25 2.683706E-6 70.0 56 CTTGCAA 25 2.683706E-6 70.0 57 CAAACTC 20 7.633407E-5 70.0 35 TCTTTAT 15 0.0021748433 70.0 1 TCACAAA 15 0.0021748433 70.0 32 TTTATAC 15 0.0021748433 70.0 3 TTTACAC 120 0.0 67.08333 2 CTATACA 80 0.0 65.625 1 CTTATAC 520 0.0 57.211536 1 ACTCGTA 25 2.3025334E-4 55.999996 38 >>END_MODULE