FastQCFastQC Report
Wed 25 May 2016
SRR1780487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97342
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1380.14176819872203159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAC254.4362707E-543.960432
TATAACA254.4362707E-543.960433
CTTATAA357.240833E-637.699741
CTTATAT301.2952904E-436.6525271
CTTTACA1000.035.1864281
TTTACAC1150.032.4924932
GATATCG353.2132395E-431.40030941
CGGTGGT1550.031.19772743
CTATACA752.1227606E-929.3220211
AAAGGGT300.005730197829.3220211
AGATATC406.9591636E-427.51769340
TCTTTAT407.001191E-427.4893951
ATAACAC407.0222834E-427.475274
TTATACA7200.026.2541472
CTTATAC7200.025.656771
TTACACA1200.025.6435833
CTTAATA450.001392125524.4350191
CTTATAG450.001392125524.4350191
TATACAC8100.024.422463
TCGGTGG2350.024.31853742
Produced by FastQC (version 0.11.5)