FastQCFastQC Report
Wed 25 May 2016
SRR1780487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97342
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3783838.87119640032052No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT2475425.42992747221138RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC71437.3380452425468965No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG54325.580325039551273No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC41504.263319019539356No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT22712.3330114441864764No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC13281.3642620862525938No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC12471.2810503174374885No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG11611.1927020196831788No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC6590.6769945141870929No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG6580.6759672083992521No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5880.6040558032503955No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5160.5300897865258573No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3890.3996219514700746No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC3860.39654003410655214No Hit
ACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCT3410.3503112736537158RNA PCR Primer, Index 32 (95% over 23bp)
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC3390.34825666207803413No Hit
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3270.3359289926239445No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3180.32668324053337716No Hit
ATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCTGCTT3080.3164101826549691TruSeq Adapter, Index 8 (96% over 26bp)
CTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT2800.2876456205954264No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTCTTC2700.2773725627170183TruSeq Adapter, Index 10 (95% over 23bp)
CTTATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC2020.20751576914384337No Hit
TCTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT1840.1890242649627088No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT1640.1684781492058926No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGTCTT1310.13457705820714594No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGC1220.12533130611657867No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG1200.12327669454089704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCA254.375731E-544.062791
CTTATAT254.375731E-544.062791
TCTTTAC254.375731E-544.062791
CTTATTA207.766173E-444.0627861
CTTACAC408.076313E-944.0627861
CTTATAA650.044.0627861
GCTTTAC207.766173E-444.0627861
TTCTGCT600.043.99486543
TCTGCTT600.043.99486544
TATATAC254.4158158E-543.994863
TTATCAC254.4158158E-543.994862
TTATTAC207.8254635E-443.994862
TTATATA254.4158158E-543.994862
TTATAAC551.8189894E-1243.994862
ATTACAC207.8254635E-443.994864
TATCACA254.4158158E-543.994863
TATTACA207.8254635E-443.994863
TCTTTAT1350.042.4308321
CCGTCTT26400.041.74512544
CTTTACA7050.041.2502751
Produced by FastQC (version 0.11.5)