FastQCFastQC Report
Wed 25 May 2016
SRR1780486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15687
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG210.13386880856760375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAACC307.5605494E-658.8897861
GAAACAC200.006572503653.0008051
GAAAAAC200.006572503653.0008051
CTGCCCG200.006828929352.4928160
TTTACAC200.006828929352.492812
GAAACCC505.27707E-849.467421
GACACAC305.440082E-447.1118281
CTTTACA350.001162278740.381571
CCGTATC350.001219025839.99452652
CCCCATC501.6360966E-434.995215
CGCCTCC400.002349069634.99520516
ACCCCAT400.002349069634.9952054
AAACCCA802.2723289E-630.6208062
CTTATAC1252.5592162E-622.6136781
TATACAC1707.952076E-820.5854173
TTATACA1205.2760028E-520.4138722
AAAACCA1858.172513E-515.1330612
TACACAT1951.2802964E-414.3570085
AAACACA1800.0011661913.6092472
ATACACA2305.184747E-412.1722464
Produced by FastQC (version 0.11.5)