FastQCFastQC Report
Wed 25 May 2016
SRR1780486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15687
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT362023.076432714986932No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT297018.9328743545611RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC9255.896602282144451No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC4432.8239943902594504No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG3792.4160132593867534No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT2581.6446739338305605No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT2231.4215592528845542No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1390.8860840186141391No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1120.71396697902722No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1080.6884681583476765No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT1020.650219927328361No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1010.6438452221584752No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT940.599222285969274No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT870.5545993497800726No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG850.5418499394403009No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT760.48447759291132786No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC670.4271052463823548No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT630.4016064257028112No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG530.33785937400395233No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGT500.3187352584942947No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC480.30598584815452284No Hit
CTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT470.299611142984637No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC420.2677376171352075No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC420.2677376171352075No Hit
ATACACATCTCCGAGCCCACGAGACTGGGCTTCATCTCGTATGCCGTCTT400.2549882067954357TruSeq Adapter, Index 11 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG400.2549882067954357No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGGTTTCATCTCGTATGCCGT360.22948938611589212No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTCTGCCGTCTT320.20399056543634855No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCG300.19124115509657677No Hit
ATACACATCTCCGAGCCCACGAGAATGGGTTTCATCTCGTATGCCGTCTT290.18486644992669088RNA PCR Primer, Index 14 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCCT270.17211703958691912No Hit
ATACACATCTCCGAGCCCACGAGACTGGGATTCATCTCGTATGCCGTCTT250.15936762924714734RNA PCR Primer, Index 39 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCT250.15936762924714734RNA PCR Primer, Index 35 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTAGTATGCCGT250.15936762924714734No Hit
ATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGTCTT220.14024351373748964RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC220.14024351373748964No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCG220.14024351373748964No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT210.13386880856760375No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCCTCTCGTATGCCGTCTT210.13386880856760375No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTC210.13386880856760375No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC200.12749410339771786No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGGTTTCATCTCGTATGCCGT190.12111939822783196No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAGCTCGTATGCCGT190.12111939822783196No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTC190.12111939822783196No Hit
ATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTGCTT190.12111939822783196RNA PCR Primer, Index 14 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCAGTCTT180.11474469305794606No Hit
CTTATACACATCTCCGAGCACACGAGACTGGGTTTCATCTCGTATGCCGT170.10836998788806018No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTAATCTCGTATGCCGT170.10836998788806018No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTCTCATCTCGTATGCCGT170.10836998788806018No Hit
TCTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT160.10199528271817428No Hit
CTTATACACATCTCCGCGCCCACGAGACTGGGTTTCATCTCGTATGCCGT160.10199528271817428No Hit
CTTATACACATCTCCGAGCCCACGTGACTGGGTTTCATCTCGTATGCCGT160.10199528271817428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA600.070.000011
CCAAAAA150.002184011370.0000170
AAATACC150.002184011370.0000170
ACCAAAA150.002184011370.0000169
TATCCGA150.002184011370.0000111
ATCCGAG150.002184011370.0000112
TCTTTAT150.002184011370.000011
TTTACAC1650.070.000012
ATATCCG150.002184011370.0000110
TATAACA207.687197E-570.03
TTATTAC207.687197E-570.02
TTATAAC207.687197E-570.02
ATAACAC207.687197E-570.04
ACACACA207.687197E-570.070
CTTATTA207.687197E-570.01
CTTATAA207.687197E-570.01
CTTTACA1750.068.01
TTACACA1950.059.230773
ATTACAC252.3186608E-456.0000044
TATTACA252.3186608E-456.0000043
Produced by FastQC (version 0.11.5)