##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15687 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69618155160324 34.0 34.0 34.0 31.0 34.0 2 33.27538726333907 34.0 34.0 34.0 32.0 34.0 3 33.4522853318034 34.0 34.0 34.0 34.0 34.0 4 33.5194109772423 34.0 34.0 34.0 34.0 34.0 5 33.576974564926374 34.0 34.0 34.0 34.0 34.0 6 37.4131446420603 38.0 38.0 38.0 37.0 38.0 7 36.919933703066235 38.0 37.0 38.0 35.0 38.0 8 36.99502772996749 38.0 37.0 38.0 36.0 38.0 9 36.65111238605215 38.0 37.0 38.0 34.0 38.0 10-11 36.39150251800854 38.0 37.0 38.0 34.0 38.0 12-13 36.094250015936765 38.0 36.5 38.0 33.0 38.0 14-15 36.104258303053484 38.0 37.0 38.0 33.0 38.0 16-17 36.03815261044177 38.0 36.0 38.0 32.5 38.0 18-19 36.175878115637154 38.0 36.5 38.0 32.5 38.0 20-21 36.03464652259833 38.0 36.5 38.0 32.0 38.0 22-23 36.00127494103398 38.0 36.5 38.0 32.5 38.0 24-25 34.686491999745016 37.0 34.0 38.0 28.0 38.0 26-27 34.820998278829606 37.0 34.0 38.0 29.0 38.0 28-29 34.908586727863835 37.0 35.0 38.0 27.0 38.0 30-31 34.27793714540702 37.0 34.0 38.0 25.0 38.0 32-33 34.887454580225665 37.0 35.0 38.0 27.0 38.0 34-35 33.81079875055779 37.0 33.5 38.0 23.5 38.0 36-37 33.80569898642187 37.0 34.0 38.0 24.0 38.0 38-39 31.537100784088736 36.0 27.0 37.0 11.0 38.0 40-41 33.2457448842991 36.5 33.0 37.5 23.5 38.0 42-43 34.78635175623128 37.0 34.5 38.0 29.0 38.0 44-45 35.18260342959138 37.0 36.0 38.0 29.0 38.0 46-47 35.13753426404028 37.0 36.0 38.0 30.0 38.0 48-49 34.756103780200164 37.0 35.0 38.0 28.0 38.0 50-51 34.67731242430038 37.0 34.0 38.0 26.5 38.0 52-53 34.33282335691974 37.0 34.0 38.0 25.0 38.0 54-55 34.7347166443552 37.0 35.0 38.0 26.5 38.0 56-57 34.58864027538726 37.0 34.0 38.0 26.5 38.0 58-59 34.38608401861414 37.0 34.0 38.0 26.0 38.0 60-61 34.35137374896411 37.0 34.0 38.0 25.5 38.0 62-63 35.17329636004335 37.0 36.0 38.0 30.5 38.0 64-65 35.08593102569006 37.5 35.5 38.0 29.0 38.0 66-67 32.96442914515204 37.0 31.0 38.0 21.5 38.0 68-69 28.794415758271178 32.5 22.0 37.5 11.0 38.0 70-71 24.973034997131382 27.0 16.0 35.5 10.5 37.5 72-73 21.47558487919934 22.0 11.0 29.5 10.5 37.0 74-75 20.943137629884617 21.0 11.0 28.0 11.0 37.0 76 21.157773952954678 21.0 11.0 28.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7943711314423325 1101 2 0.6028960888693007 1101 3 0.545464184257014 1101 4 0.4981674959504403 1101 5 -0.3590328725586218 1101 6 -0.9772687436989713 1101 7 0.5667595788434383 1101 8 0.8778038842211231 1101 9 0.03668948595523602 1101 10-11 0.012965480372393756 1101 12-13 0.7943284910373336 1101 14-15 0.755082440707973 1101 16-17 -0.3305219645543005 1101 18-19 -0.04608306819994823 1101 20-21 0.48180278506843877 1101 22-23 0.7243567131074187 1101 24-25 -3.1846188634294137 1101 26-27 -0.8689904779004038 1101 28-29 1.6832915786183023 1101 30-31 1.5184506600945653 1101 32-33 0.1501675317218627 1101 34-35 0.6401505121695976 1101 36-37 0.3145532457046727 1101 38-39 0.21313522584542355 1101 40-41 2.698011485345404 1101 42-43 1.6448950997152352 1101 44-45 0.5051922048955575 1101 46-47 1.6538459765525602 1101 48-49 -1.7177621767398819 1101 50-51 -2.562161757332454 1101 52-53 -1.584011916051061 1101 54-55 -1.098236961796374 1101 56-57 -1.2070327492320274 1101 58-59 -1.2398167359907717 1101 60-61 -2.8250039362237196 1101 62-63 -1.8143416351107575 1101 64-65 -2.293690021218829 1101 66-67 -2.52218433149924 1101 68-69 0.10151657760097166 1101 70-71 -0.923337105363295 1101 72-73 0.1409760435408316 1101 74-75 0.8649846461103863 1101 76 0.5742219544386273 1102 1 -0.6880136924059101 1102 2 -0.21282206831227057 1102 3 0.47229345254969957 1102 4 0.22987481302361346 1102 5 0.25478826565276336 1102 6 0.410942638414852 1102 7 -0.7692837815901683 1102 8 -0.015150045318172545 1102 9 0.30701468920726427 1102 10-11 0.7893882446000333 1102 12-13 1.098867786430283 1102 14-15 0.3621285111686703 1102 16-17 0.10748616552699275 1102 18-19 0.02403888301956414 1102 20-21 0.9008407254478499 1102 22-23 0.5177171467117532 1102 24-25 -4.736109378334561 1102 26-27 -0.7873509115047383 1102 28-29 0.886204857751089 1102 30-31 2.1905373809617785 1102 32-33 1.274151271559255 1102 34-35 1.5236193468579415 1102 36-37 -0.8280619304470846 1102 38-39 -0.1642360478673126 1102 40-41 1.9158299135296915 1102 42-43 1.6933368341325803 1102 44-45 1.5617640206137082 1102 46-47 1.4455126432192316 1102 48-49 -3.323453233650447 1102 50-51 0.3263883781689074 1102 52-53 -0.4664644905768043 1102 54-55 2.2676166967402125 1102 56-57 1.9386312074075391 1102 58-59 0.6768499306758926 1102 60-61 -6.652578461968979 1102 62-63 -3.667322664921052 1102 64-65 0.03490076468902714 1102 66-67 2.307083961183686 1102 68-69 -2.8517354549193534 1102 70-71 -2.6685945579378227 1102 72-73 -3.3715578317979222 1102 74-75 -2.237318876924842 1102 76 -0.6351276390573091 1103 1 0.7605547063215568 1103 2 0.714007199980415 1103 3 0.43676853208310007 1103 4 0.4329501046460891 1103 5 0.40908306947036266 1103 6 0.38867328528653644 1103 7 0.29381271894005323 1103 8 0.9816686185206436 1103 9 0.4750952830566817 1103 10-11 0.4622408426912301 1103 12-13 0.032855061085648174 1103 14-15 0.25025152283357244 1103 16-17 0.3741881803732383 1103 18-19 0.7310908448435285 1103 20-21 0.4026965048752018 1103 22-23 0.9429557469238432 1103 24-25 -3.9503193465212014 1103 26-27 -0.49519820736900755 1103 28-29 0.838484815333274 1103 30-31 1.6404313364230703 1103 32-33 2.3277037636059177 1103 34-35 1.388942782700994 1103 36-37 -0.330978155261505 1103 38-39 0.6382559987922853 1103 40-41 0.2602095529782531 1103 42-43 -0.12744789545384805 1103 44-45 1.6277767459583572 1103 46-47 1.5107300345235757 1103 48-49 -3.417037539058718 1103 50-51 -0.5977897766561213 1103 52-53 1.136398711968262 1103 54-55 0.7387195599427514 1103 56-57 1.8134986517341503 1103 58-59 0.5790238437193764 1103 60-61 -4.528506351682658 1103 62-63 -1.6392208621638957 1103 64-65 0.6181457275734417 1103 66-67 -0.5439234619340212 1103 68-69 -0.22819356732656715 1103 70-71 1.2698996096125477 1103 72-73 0.5860243527195763 1103 74-75 -0.665208590604589 1103 76 1.0488596355980455 1104 1 0.5388155758867725 1104 2 -0.025992800019587037 1104 3 0.38546418425701745 1104 4 -0.1618325040495563 1104 5 -0.5456995392252892 1104 6 0.04606458963436211 1104 7 0.5712040232878834 1104 8 0.9955816619989051 1104 9 1.1733561526218992 1104 10-11 -0.2003678529609445 1104 12-13 0.7615507132595525 1104 14-15 1.6128602184857428 1104 16-17 0.01114470211236096 1104 18-19 1.0189169318000495 1104 20-21 0.5879138961795576 1104 22-23 1.099912268662976 1104 24-25 0.873158914348366 1104 26-27 2.5104539665440413 1104 28-29 2.437180467507183 1104 30-31 2.0995617712056784 1104 32-33 2.1768341983885264 1104 34-35 1.454594956614045 1104 36-37 2.785108801260229 1104 38-39 3.2586907814009756 1104 40-41 3.2141225964565194 1104 42-43 1.072117321937455 1104 44-45 0.6690810937844489 1104 46-47 1.7155126432192276 1104 48-49 1.3355711565934527 1104 50-51 1.7539493537786583 1104 52-53 1.4798769728378218 1104 54-55 0.42176303820362193 1104 56-57 1.2274116952124103 1104 58-59 -1.2831500693241082 1104 60-61 0.9288849526651717 1104 62-63 1.071213920444798 1104 64-65 2.000754423225608 1104 66-67 2.0378156685007625 1104 68-69 2.9148499109343042 1104 70-71 -0.31444821647441046 1104 72-73 0.09819826576305246 1104 74-75 1.356095757221496 1104 76 -0.0024447122280406575 1105 1 1.1775910860908567 1105 2 0.6731908734498049 1105 3 0.13730091895089203 1105 4 -0.012036585682210443 1105 5 -0.2808015800416115 1105 6 0.4632074467772185 1105 7 0.7663060641042065 1105 8 0.15558166199890167 1105 9 1.0860092138463884 1105 10-11 0.6184076572431394 1105 12-13 0.19767316223914833 1105 14-15 0.6757173613428833 1105 16-17 1.001961028642981 1105 18-19 1.1860597889429059 1105 20-21 0.717913896179553 1105 22-23 0.4380755339690978 1105 24-25 0.9809140163891783 1105 26-27 1.0371886604215916 1105 28-29 1.222690671588822 1105 30-31 2.5377250365118016 1105 32-33 1.9545893004293404 1105 34-35 0.03357454845077257 1105 36-37 1.3471496175867586 1105 38-39 1.100323434462208 1105 40-41 1.0718776984973317 1105 42-43 0.00742344438643272 1105 44-45 0.6364280325599552 1105 46-47 1.7618391738314756 1105 48-49 0.5339385035322337 1105 50-51 2.3525207823500907 1105 52-53 2.4247749320214993 1105 54-55 2.2793140586117886 1105 56-57 1.9976157768450662 1105 58-59 0.8707274816962993 1105 60-61 1.2399053608284376 1105 62-63 -0.33531669180009516 1105 64-65 0.7578972803684749 1105 66-67 0.7635299542150449 1105 68-69 0.8787274619547105 1105 70-71 1.9145313753623228 1105 72-73 0.7994227555589717 1105 74-75 0.19548351232353767 1105 76 1.0249022265474714 1106 1 -0.8293662422950412 1106 2 0.0094617454349617 1106 3 0.5000096388024673 1106 4 0.08907658685953379 1106 5 -1.1847904483161926 1106 6 -0.18938995582018237 1106 7 -2.7842505221666656 1106 8 -1.071691065273825 1106 9 -1.751189301923553 1106 10-11 -0.41127694387003544 1106 12-13 0.4142779859868284 1106 14-15 -2.0466852360597088 1106 16-17 -0.8229462069785427 1106 18-19 -0.7960830681999482 1106 20-21 -1.0548133765477203 1106 22-23 0.1208213595720693 1106 24-25 -1.9522956311061819 1106 26-27 0.8027266938167728 1106 28-29 -0.2687286234019055 1106 30-31 -0.19998368333977368 1106 32-33 -1.8308930743387464 1106 34-35 -0.131314134295053 1106 36-37 1.2110178921693233 1106 38-39 1.6323271450373404 1106 40-41 -0.3158774035434817 1106 42-43 -0.2578826780625434 1106 44-45 0.68953563923899 1106 46-47 -0.0431237204171353 1106 48-49 0.6155711565934538 1106 50-51 -0.48514155531224645 1106 52-53 -1.0019412089803552 1106 54-55 -0.9391460527054676 1106 56-57 -0.6375883047875845 1106 58-59 0.40412265794861923 1106 60-61 0.39343040721062295 1106 62-63 0.888941193172073 1106 64-65 -0.1333364858652928 1106 66-67 1.34145203213712 1106 68-69 1.2984862745706671 1106 70-71 -0.0912663982925892 1106 72-73 -0.18225627969149372 1106 74-75 -0.273904242778503 1106 76 -1.0015356213189506 1107 1 0.5866416628432987 1107 2 0.3444419825891103 1107 3 0.43676853208310007 1107 4 0.4329501046460891 1107 5 0.4743004607747139 1107 6 0.4104124157213178 1107 7 0.6198996754617951 1107 8 0.7207990533032529 1107 9 -0.6553394995520136 1107 10-11 0.8644147557347139 1107 12-13 0.7176376697812898 1107 14-15 0.7502515228335724 1107 16-17 0.7981012238514964 1107 18-19 1.1984821491913564 1107 20-21 1.217913896179553 1107 22-23 0.7473035730108037 1107 24-25 0.7453328273918416 1107 26-27 0.6569757056744763 1107 28-29 0.6428326414202346 1107 30-31 1.009996553814375 1107 32-33 1.9037907201276525 1107 34-35 1.638942782700994 1107 36-37 2.342934888216753 1107 38-39 0.30129947705315274 1107 40-41 -0.9354426209347864 1107 42-43 1.6660303654157218 1107 44-45 1.6929941372627084 1107 46-47 1.6955126432192316 1107 48-49 1.4742668087673607 1107 50-51 2.2609058755177855 1107 52-53 1.5711813206639178 1107 54-55 1.825676081681891 1107 56-57 1.8895856082558993 1107 58-59 1.1225021045889392 1107 60-61 1.5584501700564743 1107 62-63 1.839040007401323 1107 64-65 1.6507544232256137 1107 66-67 0.07564175545727636 1107 68-69 3.07615425876039 1107 70-71 0.650334392221243 1107 72-73 -0.7291930385847749 1107 74-75 -1.632599894952417 1107 76 0.15755528777195948 1108 1 -0.2611844241132246 1108 2 0.33900719998041495 1108 3 -0.14203581574298596 1108 4 0.4356674959504403 1108 5 0.4118004607747139 1108 6 -0.03976874369897132 1108 7 -0.030462643378783127 1108 8 -3.052751671334434 1108 9 0.03668948595523602 1108 10-11 0.34629881370572946 1108 12-13 0.985300713259555 1108 14-15 0.9495268851524159 1108 16-17 -0.3721886312209719 1108 18-19 0.058083598466723174 1108 20-21 0.48874722951288163 1108 22-23 -0.8034210646703528 1108 24-25 1.2285755810150363 1108 26-27 -0.9037127001226324 1108 28-29 -0.8236528658261406 1108 30-31 0.4420617712056796 1108 32-33 0.848084198388527 1108 34-35 -0.2695717100526309 1108 36-37 0.9117754679268941 1108 38-39 1.529107448067652 1108 40-41 1.6632892631231826 1108 42-43 0.315033988604128 1108 44-45 1.0919977604511075 1108 46-47 1.3830126432192316 1108 48-49 1.3551544899267896 1108 50-51 1.3093660204453315 1108 52-53 -0.05970636049550393 1108 54-55 -0.9315702951297027 1108 56-57 0.26866169521241545 1108 58-59 -0.1460667359907788 1108 60-61 1.199301619331841 1108 62-63 0.8245472537781353 1108 64-65 0.19242108989227802 1108 66-67 0.8319823351674316 1108 68-69 2.184849910934304 1108 70-71 -0.3781982164744093 1108 72-73 -0.7722184009036113 1108 74-75 -0.15932090944517086 1108 76 -1.0716113788947084 1109 1 -1.2101640159499603 1109 2 -0.24517647348897498 1109 3 0.320974388338648 1109 4 0.29408586329737574 1109 5 0.1681780117951206 1109 6 -0.5776088797533916 1109 7 -0.11124495630395614 1109 8 -0.27298976657252894 1109 9 0.4737643158872089 1109 10-11 0.363305616426814 1109 12-13 0.758897652035067 1109 14-15 1.0430643001183952 1109 16-17 1.1958385796633877 1109 18-19 0.34932509506536036 1109 20-21 1.2485261410775124 1109 22-23 1.0605245135609351 1109 24-25 -0.4272492489169366 1109 26-27 0.8637192726664935 1109 28-29 -0.3181256549417881 1109 30-31 -0.2888055757331003 1109 32-33 0.34234440247016096 1109 34-35 -1.6398948393043256 1109 36-37 1.010414923709206 1109 38-39 1.2533846589520046 1109 40-41 0.15351035155855897 1109 42-43 1.3849744647945954 1109 44-45 0.9119382366415891 1109 46-47 0.649594275872289 1109 48-49 0.9216936055730471 1109 50-51 -0.2801322788743974 1109 52-53 -0.514000578182582 1109 54-55 -1.7104818597555607 1109 56-57 -0.5738127945835032 1109 58-59 -1.0272317019771648 1109 60-61 1.1480686261345525 1109 62-63 0.4606016755468403 1109 64-65 1.6252442191439798 1109 66-67 0.7941421991130042 1109 68-69 -0.5906602931473302 1109 70-71 0.516572191688855 1109 72-73 0.14636153106917504 1109 74-75 -0.28410832441115375 1109 76 -0.4240773652892642 1110 1 -0.33896220189100035 1110 2 -0.9304372444640308 1110 3 0.10101973981257117 1110 4 0.25372305150599317 1110 5 0.007633794108045322 1110 6 -0.3717131881434099 1110 7 0.5667595788434383 1110 8 0.6222483286655702 1110 9 0.9200228192885689 1110 10-11 0.05740992481683804 1110 12-13 1.024884046592888 1110 14-15 1.321749107374636 1110 16-17 0.8944780354457009 1110 18-19 0.12891693180004893 1110 20-21 0.8290250072906673 1110 22-23 -0.3811988424481356 1110 24-25 -0.44017441898496656 1110 26-27 1.1115650776551504 1110 28-29 0.5027360230627451 1110 30-31 -0.9482160065720961 1110 32-33 -0.3553880238336973 1110 34-35 2.051261623280709 1110 36-37 2.6451088012602284 1110 38-39 0.29646855917875925 1110 40-41 0.38412259645652114 1110 42-43 0.6976728774930123 1110 44-45 1.5246366493400032 1110 46-47 1.6232904209970087 1110 48-49 1.4933489343712338 1110 50-51 0.057282687111992914 1110 52-53 1.7465436395044875 1110 54-55 0.2573185937591802 1110 56-57 1.6318561396568612 1110 58-59 -1.6787056248796617 1110 60-61 -0.10000393622372172 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-0.8242729420716728 2116 62-63 0.7412139204447996 2116 64-65 -2.7176666294059686 2116 66-67 -1.653763278867661 2116 68-69 0.42169201619746133 2116 70-71 3.360288625630851 2116 72-73 0.5013561604998955 2116 74-75 -0.5107463480416605 2116 76 0.920713182508802 2117 1 0.48205881913001747 2117 2 0.6599531459263588 2117 3 0.4914101302029579 2117 4 0.06573506351800518 2117 5 0.44727343374768935 2117 6 0.443902427472203 2117 7 0.6598526719365267 2117 8 0.9934194998367403 2117 9 -0.5466438473780997 2117 10-11 -0.13117866377174892 2117 12-13 -2.1354763137674766 2117 14-15 -0.02119383556831167 2117 16-17 -0.011828270860604562 2117 18-19 -0.495407392524271 2117 20-21 -0.403707725442068 2117 22-23 0.47585821460892674 2117 24-25 -2.663597842408393 2117 26-27 -2.407654141564066 2117 28-29 -1.742008721682005 2117 30-31 0.0619942036381147 2117 32-33 -1.7304630989087713 2117 34-35 2.0710814431005247 2117 36-37 -1.9170533609019316 2117 38-39 -1.035363272653072 2117 40-41 -0.8699314575975308 2117 42-43 0.5394146192347478 2117 44-45 -0.39308106837771817 2117 46-47 -0.5274603297537368 2117 48-49 -0.4384828974606023 2117 50-51 -0.7246992948699855 2117 52-53 1.4366337295945826 2117 54-55 0.645006281446868 2117 56-57 0.40633061413133476 2117 58-59 -2.931258177432216 2117 60-61 -2.459763695983476 2117 62-63 -0.4141914849606039 2117 64-65 -0.8289753065041126 2117 66-67 -1.832995142310054 2117 68-69 -1.5989338728494786 2117 70-71 -1.0749887570149497 2117 72-73 -2.2753152477504592 2117 74-75 -0.8279582968325592 2117 76 -0.39649876628209313 2118 1 0.0888155758867768 2118 2 0.4390071999804164 2118 3 -0.5295358157429888 2118 4 0.4731674959504417 2118 5 0.4743004607747139 2118 6 0.40606458963436154 2118 7 0.7612040232878812 2118 8 0.9305816619989002 2118 9 -1.896643847378101 2118 10-11 -0.2078678529609448 2118 12-13 0.4040507132595579 2118 14-15 0.9703602184857445 2118 16-17 -1.801355297887639 2118 18-19 -0.4460830681999539 2118 20-21 0.4929138961795516 2118 22-23 1.2924122686629786 2118 24-25 1.268158914348362 2118 26-27 -2.974546033455958 2118 28-29 -0.27781953249281344 2118 30-31 -0.7329382287943176 2118 32-33 0.6543341983885327 2118 34-35 0.8720949566140419 2118 36-37 -2.079891198739773 2118 38-39 0.3936907814009807 2118 40-41 1.4341225964565183 2118 42-43 1.4171173219374538 2118 44-45 -1.5684189062155625 2118 46-47 0.9455126432192316 2118 48-49 1.1905711565934567 2118 50-51 -0.6885506462213371 2118 52-53 -1.7576230271621753 2118 54-55 -1.3982369617963712 2118 56-57 1.4499116952124211 2118 58-59 0.41434993067588977 2118 60-61 0.02638495266516827 2118 62-63 -1.4587860795552032 2118 64-65 -0.3242455767743877 2118 66-67 0.46531566850075734 2118 68-69 0.28484991093430523 2118 70-71 2.04055178352559 2118 72-73 1.0756982657630516 2118 74-75 5.001095757221496 2118 76 2.4075552877719595 2119 1 0.4388155758867782 2119 2 -1.4859928000195843 2119 3 -0.7973929586001276 2119 4 -1.4161182183352707 2119 5 -0.23998525351100142 2119 6 -0.3939354103656356 2119 7 -1.5030816909978313 2119 8 -0.215846909429672 2119 9 -0.48950099023524274 2119 10-11 -0.27751071010379746 2119 12-13 -0.4084492867404421 2119 14-15 -0.6385683529428263 2119 16-17 -0.8102838693162084 2119 18-19 -0.41394021105708845 2119 20-21 -3.610657532391876 2119 22-23 -0.6272305884798826 2119 24-25 0.07887320006265242 2119 26-27 -1.4281174620273944 2119 28-29 -1.6813909610642384 2119 30-31 -0.18472394308003715 2119 32-33 -0.9617372301828979 2119 34-35 -0.30111932910024564 2119 36-37 -4.226319770168342 2119 38-39 -5.752737790027592 2119 40-41 -2.9301631178291956 2119 42-43 -1.8864541066339697 2119 44-45 -0.6880617633584123 2119 46-47 -0.983058785352199 2119 48-49 -0.9130002719779711 2119 50-51 0.6810922109215127 2119 52-53 -1.6772658843050365 2119 54-55 -2.2839512475106574 2119 56-57 -3.526874019073297 2119 58-59 -0.837435783609827 2119 60-61 0.3603135240937405 2119 62-63 -1.908786079555199 2119 64-65 -1.1135312910601058 2119 66-67 0.9492442399293353 2119 68-69 2.1562784823628753 2119 70-71 -0.5915910736172663 2119 72-73 -0.03323030566551921 2119 74-75 0.026095757221497706 2119 76 -1.5424447122280398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 1.0 22 3.0 23 10.0 24 40.0 25 67.0 26 112.0 27 246.0 28 398.0 29 567.0 30 884.0 31 1269.0 32 1834.0 33 2526.0 34 3196.0 35 2834.0 36 921.0 37 778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.584241728820042 29.871868426085296 10.970867597373621 53.57302224772105 2 2.976987314336712 5.32287881685472 81.53885382801045 10.161280040798113 3 2.5307579524446995 34.525403200102 57.82495059603493 5.1188882514183724 4 1.7721680372282782 41.543953592146366 24.95697074010327 31.72690763052209 5 1.5171798304328425 50.67890610059285 42.65315229170651 5.150761777267801 6 3.1299802384139737 45.687511952572194 8.943711353349908 42.23879645566392 7 3.1746031746031744 50.64065786957354 7.401032702237521 38.78370625358577 8 2.9961114298463696 44.57831325301205 29.80174666921655 22.623828647925034 9 3.0216102505259133 22.540957480716518 5.5268693822910695 68.9105628864665 10-11 2.906865557467967 25.422324217504936 41.37502390514439 30.295786319882705 12-13 16.449926690890546 5.4854337986868105 31.972333779562696 46.09230573085995 14-15 24.064512016319245 17.788614776566583 8.701472556894243 49.44540065021993 16-17 30.856760374832664 26.99368904188181 3.3052846305858354 38.844265952699686 18-19 24.81672722636578 22.438962197998343 3.4200293236437815 49.3242812519921 20-21 18.397399120290686 12.000382482310194 3.2670363995665204 66.3351819978326 22-23 24.54580225664563 38.40122394339262 2.830369095429336 34.222604704532415 24-25 30.738828329189776 43.13762988461784 3.898132211385223 22.225409574807166 26-27 38.45540893733665 31.86396379167464 18.072289156626507 11.608338114362212 28-29 40.8491107286288 9.128577803276599 26.888506406578692 23.133805061515904 30-31 53.71964046662841 3.843947217441193 36.41231593038822 6.024096385542169 32-33 30.391406897430993 3.936380442404539 49.2063492063492 16.46586345381526 34-35 5.428061452157838 18.735258494294637 64.3239625167336 11.512717536813923 36-37 3.2001019952827185 27.596098680436032 32.00739465799707 37.19640466628418 38-39 16.201313189264997 11.079237585261682 30.155542806145213 42.563906419328106 40-41 10.81787467329636 17.60374832663989 40.22120226939504 31.357174730668707 42-43 35.95333715815644 11.31510167654746 43.69541658698285 9.036144578313253 44-45 12.717536813922356 23.19436476062982 32.53968253968254 31.548415885765284 46-47 37.40039523172054 5.291005291005291 22.611079237585262 34.69752023968892 48-49 27.86702365015618 3.639956652004845 25.457385095939312 43.035634601899666 50-51 8.076751450245427 3.67820488302416 46.37598011091987 41.86906355581054 52-53 15.726397654108498 3.8917575062153373 63.69924141008478 16.68260342959138 54-55 10.480015299292408 3.8535092751960223 47.666857907821765 37.999617517689806 56-57 35.52304455918914 4.213680117294575 31.48148148148148 28.78179384203481 58-59 12.421113023522661 31.628099700388855 46.72021419009371 9.230573085994772 60-61 22.193536048957736 62.14700070121757 11.162108752470198 4.497354497354497 62-63 4.296551284503092 87.85299929878244 3.8088863390068206 4.041563077707655 64-65 2.352266207687894 89.88971760056097 3.273411104736406 4.484605087014726 66-67 2.314017976668579 87.89443488238669 3.381781092624466 6.4097660483202645 68-69 2.922802320392682 78.77223178427998 5.096576783323771 13.208389112003568 70-71 4.070249250972142 70.41499330655957 7.6368967935233 17.87786064894499 72-73 5.141199719512972 58.70784726206413 10.86249760948556 25.288455408937338 74-75 5.995410212277682 56.23764900873335 11.133422579205712 26.633518199783264 76 4.946771211831453 58.44329699751386 10.41626824759355 26.193663543061135 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 1.0 19 1.0 20 1.0 21 0.0 22 1.0 23 1.0 24 2.0 25 4.0 26 3.5 27 5.0 28 9.5 29 12.0 30 9.5 31 13.5 32 27.0 33 34.0 34 34.0 35 41.5 36 58.5 37 68.0 38 107.0 39 347.0 40 1403.0 41 2924.0 42 3590.0 43 3499.0 44 2872.0 45 1840.0 46 1344.0 47 1040.5 48 540.5 49 262.5 50 181.0 51 157.5 52 115.5 53 83.5 54 70.0 55 72.5 56 63.0 57 40.0 58 29.0 59 25.0 60 20.5 61 15.0 62 10.0 63 12.0 64 12.5 65 9.0 66 5.5 67 4.0 68 5.5 69 3.5 70 0.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 15687.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.023777650283673 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65251667677381 16.115254669471536 2 12.067919951485749 5.07426531522917 3 4.396604002425713 2.7729967489003635 4 1.8192844147968468 1.5299292407726142 5 0.8489993935718617 0.892458723784025 6 0.667070952092177 0.8414610824249378 7 0.4851425106124924 0.71396697902722 8 0.27289266221952696 0.45897877223178424 9 0.3941782898726501 0.7458405048766494 >10 1.8192844147968468 7.713393255561931 >50 0.21224984839296543 3.3467202141900936 >100 0.27289266221952696 11.888825141837192 >500 0.030321406913280776 5.896602282144451 >1k 0.06064281382656155 42.009307069548036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 3620 23.076432714986932 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 2970 18.9328743545611 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 925 5.896602282144451 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 443 2.8239943902594504 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 379 2.4160132593867534 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 258 1.6446739338305605 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 223 1.4215592528845542 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 139 0.8860840186141391 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 112 0.71396697902722 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 108 0.6884681583476765 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 102 0.650219927328361 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 101 0.6438452221584752 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 94 0.599222285969274 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT 87 0.5545993497800726 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 85 0.5418499394403009 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 76 0.48447759291132786 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 67 0.4271052463823548 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT 63 0.4016064257028112 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 53 0.33785937400395233 No Hit CTTATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGT 50 0.3187352584942947 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 48 0.30598584815452284 No Hit CTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 47 0.299611142984637 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 42 0.2677376171352075 No Hit CTTATATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 42 0.2677376171352075 No Hit ATACACATCTCCGAGCCCACGAGACTGGGCTTCATCTCGTATGCCGTCTT 40 0.2549882067954357 TruSeq Adapter, Index 11 (95% over 23bp) CTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 40 0.2549882067954357 No Hit CTTATACACATCTCCGAGCCCACGCGACTGGGTTTCATCTCGTATGCCGT 36 0.22948938611589212 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTCTGCCGTCTT 32 0.20399056543634855 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCG 30 0.19124115509657677 No Hit ATACACATCTCCGAGCCCACGAGAATGGGTTTCATCTCGTATGCCGTCTT 29 0.18486644992669088 RNA PCR Primer, Index 14 (95% over 22bp) ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCCT 27 0.17211703958691912 No Hit ATACACATCTCCGAGCCCACGAGACTGGGATTCATCTCGTATGCCGTCTT 25 0.15936762924714734 RNA PCR Primer, Index 39 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCT 25 0.15936762924714734 RNA PCR Primer, Index 35 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTAGTATGCCGT 25 0.15936762924714734 No Hit ATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGTCTT 22 0.14024351373748964 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC 22 0.14024351373748964 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCG 22 0.14024351373748964 No Hit TCTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 21 0.13386880856760375 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCCTCTCGTATGCCGTCTT 21 0.13386880856760375 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTC 21 0.13386880856760375 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC 20 0.12749410339771786 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGGTTTCATCTCGTATGCCGT 19 0.12111939822783196 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAGCTCGTATGCCGT 19 0.12111939822783196 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTC 19 0.12111939822783196 No Hit ATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTGCTT 19 0.12111939822783196 RNA PCR Primer, Index 14 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCAGTCTT 18 0.11474469305794606 No Hit CTTATACACATCTCCGAGCACACGAGACTGGGTTTCATCTCGTATGCCGT 17 0.10836998788806018 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTAATCTCGTATGCCGT 17 0.10836998788806018 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTCTCATCTCGTATGCCGT 17 0.10836998788806018 No Hit TCTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 16 0.10199528271817428 No Hit CTTATACACATCTCCGCGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 16 0.10199528271817428 No Hit CTTATACACATCTCCGAGCCCACGTGACTGGGTTTCATCTCGTATGCCGT 16 0.10199528271817428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.006374705169885893 0.0 3 0.0 0.0 0.0 0.006374705169885893 0.0 4 0.0 0.0 0.0 0.012749410339771786 0.0 5 0.0 0.0 0.0 0.012749410339771786 0.0 6 0.0 0.0 0.0 0.012749410339771786 0.0 7 0.0 0.0 0.0 0.012749410339771786 0.0 8 0.0 0.0 0.0 0.012749410339771786 0.0 9 0.0 0.0 0.0 0.012749410339771786 0.0 10 0.0 0.0 0.0 0.012749410339771786 0.0 11 0.0 0.0 0.0 0.012749410339771786 0.0 12 0.0 0.0 0.0 0.012749410339771786 0.0 13 0.0 0.0 0.0 0.019124115509657678 0.0 14 0.0 0.0 0.0 0.019124115509657678 0.0 15 0.0 0.0 0.0 0.025498820679543573 0.0 16 0.0 0.0 0.0 0.025498820679543573 0.0 17 0.0 0.0 0.0 0.031873525849429464 0.0 18 0.0 0.0 0.0 0.038248231019315355 0.0 19 0.0 0.0 0.0 0.05737234652897304 0.0 20 0.0 0.0 0.0 0.05737234652897304 0.0 21 0.0 0.0 0.0 0.05737234652897304 0.0 22 0.0 0.0 0.0 0.05737234652897304 0.0 23 0.0 0.0 0.0 0.05737234652897304 0.0 24 0.0 0.0 0.0 0.05737234652897304 0.0 25 0.0 0.0 0.0 0.07649646203863071 0.0 26 0.0 0.0 0.0 0.07649646203863071 0.0 27 0.0 0.0 0.0 0.07649646203863071 0.0 28 0.0 0.0 0.0 0.0828711672085166 0.0 29 0.0 0.0 0.0 0.0828711672085166 0.0 30 0.0 0.0 0.0 0.0828711672085166 0.0 31 0.0 0.0 0.0 0.09562057754828839 0.0 32 0.0 0.0 0.0 0.10836998788806018 0.0 33 0.0 0.0 0.0 0.13386880856760375 0.0 34 0.0 0.0 0.0 0.1657423344170332 0.0 35 0.0 0.0 0.0 0.1657423344170332 0.0 36 0.0 0.0 0.0 0.21673997577612036 0.0 37 0.0 0.0 0.0 0.2804870274749793 0.0 38 0.0 0.0 0.0 0.33785937400395233 0.0 39 0.0 0.0 0.0 0.36973289985338176 0.0 40 0.0 0.0 0.0 0.5036017084209855 0.0 41 0.0 0.0 0.0 0.6310958118187033 0.0 42 0.0 0.0 0.0 0.7777140307260789 0.0 43 0.0 0.0 0.0 0.8860840186141391 0.0 44 0.0 0.0 0.0 1.0263275323516288 0.0 45 0.0 0.0 0.0 1.198444571938548 0.0 46 0.0 0.0 0.0 1.3578122011856952 0.0 47 0.0 0.0 0.0 1.4598074839038695 0.0 48 0.0 0.0 0.0 1.5044304200930707 0.0 49 0.0 0.0 0.0 1.5681774717919297 0.0 50 0.0 0.0 0.0 1.6191751131510168 0.0 51 0.0 0.0 0.0 1.7721680372282782 0.0 52 0.0 0.0 0.0 1.9442850768151974 0.0 53 0.0 0.0 0.0 2.0590297698731432 0.0 54 0.0 0.0 0.0 2.129151526741888 0.0 55 0.0 0.0 0.0 2.212022693950405 0.0 56 0.0 0.0 0.0 2.339516797348123 0.0 57 0.0 0.0 0.0 2.422387964556639 0.0 58 0.0 0.0 0.0 2.479760311085612 0.0 59 0.0 0.0 0.0 2.562631478294129 0.0 60 0.0 0.0 0.0 2.6837508765219606 0.0 61 0.0 0.0 0.0 2.7602473385605917 0.0 62 0.0 0.0 0.0 2.843118505769108 0.0 63 0.0 0.0 0.0 2.8813667367884235 0.0 64 0.0 0.0 0.0 2.970612609166826 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 60 0.0 70.00001 1 CCAAAAA 15 0.0021840113 70.00001 70 AAATACC 15 0.0021840113 70.00001 70 ACCAAAA 15 0.0021840113 70.00001 69 TATCCGA 15 0.0021840113 70.00001 11 ATCCGAG 15 0.0021840113 70.00001 12 TCTTTAT 15 0.0021840113 70.00001 1 TTTACAC 165 0.0 70.00001 2 ATATCCG 15 0.0021840113 70.00001 10 TATAACA 20 7.687197E-5 70.0 3 TTATTAC 20 7.687197E-5 70.0 2 TTATAAC 20 7.687197E-5 70.0 2 ATAACAC 20 7.687197E-5 70.0 4 ACACACA 20 7.687197E-5 70.0 70 CTTATTA 20 7.687197E-5 70.0 1 CTTATAA 20 7.687197E-5 70.0 1 CTTTACA 175 0.0 68.0 1 TTACACA 195 0.0 59.23077 3 ATTACAC 25 2.3186608E-4 56.000004 4 TATTACA 25 2.3186608E-4 56.000004 3 >>END_MODULE