FastQCFastQC Report
Wed 25 May 2016
SRR1780485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94072
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1290.1371290075686708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC207.860696E-443.9532178
TGTTGGC250.002317419735.27509320
CCCTTGC250.002335686235.21874637
TTATGCT250.002354066635.162579
TATGCTT353.2054968E-431.41185410
CTTTACA1150.030.641311
CGGTGGT1150.030.5761543
CTCTACA300.00568871629.364591
TATCACA300.00574826929.3021453
TATATTA300.00574826929.3021455
ATAGACA300.00574826929.3021454
TTATCAC300.00574826929.3021452
TATTATG300.00574826929.3021457
ATTATGC300.00574826929.3021458
ATATTAT300.00574826929.3021456
TTACACA1350.029.3021433
GGTGGTC1150.028.66514244
CTATACA703.480818E-828.3158551
CTTATAC5850.027.8587131
TTTACAC1350.027.6742482
Produced by FastQC (version 0.11.5)