##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780485_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94072 Sequences flagged as poor quality 0 Sequence length 50 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.973658474360064 16.0 10.0 30.0 7.0 33.0 2 19.970788332341186 18.0 10.0 30.0 7.0 33.0 3 20.237934773365083 22.0 10.0 30.0 7.0 33.0 4 22.229271196530316 22.0 10.0 33.0 7.0 37.0 5 22.140509397057574 22.0 10.0 33.0 7.0 37.0 6 22.50582532528276 24.0 10.0 33.0 8.0 37.0 7 22.651713581086828 25.0 11.0 35.0 8.0 37.0 8 22.57992814014797 25.0 10.0 35.0 8.0 37.0 9 23.083446721660007 25.0 10.0 35.0 8.0 39.0 10 22.838506675737733 24.0 10.0 34.0 8.0 39.0 11 22.901033251126798 24.0 10.0 35.0 8.0 39.0 12 22.791053661025597 23.0 10.0 34.0 8.0 39.0 13 22.674982991750998 23.0 10.0 34.0 8.0 39.0 14 23.060815120333363 23.0 10.0 34.0 8.0 40.0 15 22.96569648779658 23.0 10.0 34.0 8.0 40.0 16 22.775002126031126 22.0 10.0 34.0 8.0 40.0 17 22.80846585594013 23.0 10.0 34.0 8.0 40.0 18 22.647769793349774 22.0 10.0 34.0 7.0 40.0 19 22.631686367888427 22.0 10.0 34.0 7.0 40.0 20 22.70486435921422 22.0 10.0 34.0 7.0 40.0 21 22.527468322136237 22.0 10.0 34.0 7.0 40.0 22 22.588581086827112 22.0 10.0 34.0 7.0 40.0 23 22.429181903223064 22.0 10.0 34.0 7.0 40.0 24 22.022365847436006 20.0 9.0 34.0 7.0 40.0 25 21.969374521642997 20.0 9.0 34.0 7.0 40.0 26 21.661355132239137 19.0 8.0 33.0 7.0 40.0 27 21.508578535589763 19.0 8.0 33.0 7.0 40.0 28 21.359586274343055 18.0 8.0 33.0 7.0 40.0 29 21.16578790713496 18.0 8.0 32.0 7.0 40.0 30 20.988030444765712 18.0 8.0 32.0 7.0 40.0 31 20.91638319584999 18.0 8.0 32.0 7.0 40.0 32 20.639754656008165 17.0 8.0 32.0 7.0 40.0 33 20.621321966153584 17.0 8.0 32.0 7.0 40.0 34 20.29528446296454 16.0 8.0 31.0 7.0 40.0 35 20.15478569606259 16.0 8.0 31.0 7.0 40.0 36 20.04088357853559 16.0 8.0 31.0 7.0 40.0 37 19.86543285993707 15.0 8.0 30.0 7.0 40.0 38 19.879145760693937 15.0 8.0 30.0 7.0 40.0 39 19.634312016327918 15.0 8.0 30.0 7.0 40.0 40 19.133068288119738 15.0 8.0 30.0 7.0 40.0 41 19.2855047197891 15.0 8.0 30.0 7.0 40.0 42 19.29584786121269 15.0 8.0 30.0 7.0 40.0 43 19.316427842503614 15.0 8.0 30.0 7.0 40.0 44 19.28457989624968 15.0 8.0 30.0 7.0 40.0 45 19.285738583212858 15.0 8.0 30.0 7.0 39.0 46 19.14805680755166 14.0 8.0 30.0 7.0 39.0 47 19.06636406156986 14.0 8.0 29.0 7.0 39.0 48 19.051811378518583 14.0 8.0 29.0 7.0 39.0 49 18.729324347308445 13.0 8.0 29.0 7.0 39.0 50 18.71456969130028 13.0 8.0 28.0 7.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8554781400927318 1101 2 0.9479573641570198 1101 3 1.2020265274253177 1101 4 1.1434023290945774 1101 5 0.8528668910953563 1101 6 1.1794229268981837 1101 7 1.0242432336258709 1101 8 0.6732137017688551 1101 9 1.926507759774207 1101 10 1.6822462906422864 1101 11 0.5480585877180211 1101 12 1.7165443730101408 1101 13 1.8521957373907014 1101 14 0.6205573122914565 1101 15 0.7255519598087758 1101 16 1.389732269613031 1101 17 1.5170747746354465 1101 18 2.1643621219381437 1101 19 1.9865781476322972 1101 20 2.3556613097818158 1101 21 1.140415937118469 1101 22 1.149874743037369 1101 23 2.2328724511832583 1101 24 0.31108740448713235 1101 25 0.3693004525084156 1101 26 1.0949243533649167 1101 27 0.36258931808330175 1101 28 -0.21958949700345798 1101 29 1.3214690359545145 1101 30 1.3789544541972276 1101 31 1.3068361934916588 1101 32 1.4484281119574263 1101 33 1.1166784567450385 1101 34 0.6629704356825705 1101 35 0.46575360933338317 1101 36 0.4419417217847723 1101 37 0.13066688200921206 1101 38 -0.13336655693523625 1101 39 1.0646204339760779 1101 40 1.5592480430419364 1101 41 0.6617574627927603 1101 42 1.166878371981408 1101 43 1.697783512494592 1101 44 0.8812652060429578 1101 45 1.1879919175672562 1101 46 0.6504646910707024 1101 47 -0.11400139351549043 1101 48 1.4476762060441217 1101 49 1.4564673764675078 1101 50 1.2843762434804233 1102 1 1.1453332125565012 1102 2 1.5421602627077462 1102 3 0.3107221795992281 1102 4 1.5347066769206634 1102 5 0.6717074708055009 1102 6 0.6721765500865899 1102 7 1.2778664220316678 1102 8 1.2166919626384178 1102 9 -0.04450673297941421 1102 10 1.7039854210770677 1102 11 -0.07512981807908048 1102 12 0.21654437301014084 1102 13 0.6637899402892522 1102 14 0.9756297760595736 1102 15 0.863233119229065 1102 16 0.7882829942507108 1102 17 0.5388139050702314 1102 18 0.5701592233874209 1102 19 1.1604911911105589 1102 20 0.9643569619557297 1102 21 1.2780970965387581 1102 22 0.5484254676750488 1102 23 -0.013504360410944827 1102 24 0.9487685639074215 1102 25 0.746112046711314 1102 26 0.5949243533649167 1102 27 0.6669371441702587 1102 28 0.20794673488060056 1102 29 -0.9394005292628762 1102 30 1.3064906860812862 1102 31 0.6474159036365847 1102 32 -0.6602675402164877 1102 33 0.5152291813827183 1102 34 1.0687675371318441 1102 35 0.5454637542609184 1102 36 -0.07979740865000906 1102 37 0.6524060124439934 1102 38 1.6130102546589669 1102 39 0.5936059412224566 1102 40 -0.10741862362472787 1102 41 -0.49041645025071645 1102 42 -0.20268684540989668 1102 43 -0.5413469222880174 1102 44 0.6421347712603485 1102 45 0.34741220742233025 1102 46 0.7301748359982376 1102 47 0.3352739688033495 1102 48 -0.22623683743413991 1102 49 0.014438390960261671 1102 50 0.9365501565239001 1103 1 0.4283745277198143 1103 2 0.6439340027034177 1103 3 0.5913840874512744 1103 4 0.47003006000524294 1103 5 1.6091939514457785 1103 6 0.6556288537854869 1103 7 1.3608212132927555 1103 8 0.7955368685436746 1103 9 0.3261400331897377 1103 10 0.9675588582390837 1103 11 -0.9837389463507655 1103 12 0.5374398953982009 1103 13 1.249664913250534 1103 14 1.7775981948352602 1103 15 1.6414074648920547 1103 16 1.5480710106902542 1103 17 0.7978448373652789 1103 18 0.9223115054553404 1103 19 1.5032339988111865 1103 20 2.186615235803881 1103 21 0.9796977887299754 1103 22 1.5747071029768023 1103 23 1.7534218779624062 1103 24 1.4248663359169385 1103 25 1.1347125225927783 1103 26 2.134725348389793 1103 27 1.24740437324229 1103 28 1.747423265272122 1103 29 0.48651337945440787 1103 30 1.4255692065225567 1103 31 1.3646990531066265 1103 32 0.8738012462857832 1103 33 1.0120926899269556 1103 34 0.802165763391848 1103 35 0.7734542366316752 1103 36 1.4555692363856814 1103 37 1.0459816127035637 1103 38 0.6193913924482821 1103 39 1.3228942821266294 1103 40 0.6267366867797683 1103 41 0.9161377353430531 1103 42 -0.08241862131298916 1103 43 -0.025015535742486605 1103 44 -0.6745427108940945 1103 45 -0.3787396420260798 1103 46 -0.10845808634655896 1103 47 0.6265350546783246 1103 48 1.5161382436820183 1103 49 0.350800060871574 1103 50 1.3005994751481715 1104 1 -1.2409132615218965 1104 2 -0.774978147952698 1104 3 -0.8448074381422366 1104 4 -0.755002495338843 1104 5 -0.6837540601739747 1104 6 -0.46307293926269466 1104 7 -0.2343405617247747 1104 8 -1.7484378885433713 1104 9 -0.9561398425991037 1104 10 -0.8354562674746298 1104 11 -0.8397441552058176 1104 12 -1.8170126739697245 1104 13 -1.5519478284102917 1104 14 -0.8919901052749282 1104 15 -0.7542651786018837 1104 16 -0.28374307325244885 1104 17 -0.5867089039950351 1104 18 -0.2638549775657957 1104 19 -0.41031904135710207 1104 20 -0.7312952119573133 1104 21 -0.8178079710616686 1104 22 0.01530612130796527 1104 23 -1.509224621085977 1104 24 -0.12802357693490762 1104 25 -1.0287153047136428 1104 26 -0.570623745069085 1104 27 -0.9649566404810379 1104 28 -1.4249197177990993 1104 29 -1.3417446592113222 1104 30 -0.3621699500436044 1104 31 -1.022509200925228 1104 32 -0.1153302773043201 1104 33 -1.4015104353860757 1104 34 -0.9816166667393738 1104 35 -0.9311435796560161 1104 36 -0.7461236415067347 1104 37 -0.5206316298087046 1104 38 -0.911160545846819 1104 39 -0.0506407273895455 1104 40 -0.20162152217545426 1104 41 -0.78280045958833 1104 42 -1.2987864466160026 1104 43 -0.9961664923687508 1104 44 -0.9001762879751318 1104 45 -1.6352256682706958 1104 46 -1.7863605204845925 1104 47 -1.111569723444486 1104 48 -0.9145940011341693 1104 49 -0.8342144638204019 1104 50 -0.07453825899987976 1105 1 -0.305728927606296 1105 2 0.10876737011294679 1105 3 0.5284112802543817 1105 4 0.5849349874229475 1105 5 0.04673228716444555 1105 6 -0.7495030210867277 1105 7 0.5023055723195391 1105 8 -0.31159868655753087 1105 9 -0.21117339964608206 1105 10 0.5146862350536416 1105 11 0.14067323929634057 1105 12 0.2713388935580845 1105 13 -0.16507644843419556 1105 14 -0.4896273214190856 1105 15 -0.29807321390573804 1105 16 -1.0584511729122816 1105 17 0.1934694540080919 1105 18 0.41004407548192034 1105 19 0.12038795505735322 1105 20 0.5700746510563839 1105 21 0.8395424012836479 1105 22 0.21936054808005068 1105 23 1.3389872020270133 1105 24 0.1433288180269372 1105 25 0.6903248718056183 1105 26 0.6930978693466514 1105 27 0.14936716203803613 1105 28 0.3683596790934267 1105 29 0.4774150355574527 1105 30 1.4098260047233353 1105 31 0.6663756018696603 1105 32 -0.05157188804257373 1105 33 0.007287946520698085 1105 34 0.8869132587915622 1105 35 -0.09221740515937071 1105 36 0.5402145032022823 1105 37 0.4710480612825876 1105 38 0.8958174811826929 1105 39 0.7617615894257526 1105 40 0.59289902576975 1105 41 1.4002923049607183 1105 42 1.4327112089875627 1105 43 0.28483929966949617 1105 44 1.3467208343931674 1105 45 0.9885676570947552 1105 46 1.1188982824941682 1105 47 1.691041290621893 1105 48 1.1850198629827737 1105 49 0.40385669550661873 1105 50 -0.019375989991882392 1106 1 -0.40411841298208273 1106 2 -0.22536813525543664 1106 3 -0.4157760577685714 1106 4 0.1270490192630085 1106 5 -0.903008549562692 1106 6 0.631929781578954 1106 7 0.5123944282988049 1106 8 1.0078200472447634 1106 9 0.10639416792148637 1106 10 -0.28113012925587455 1106 11 0.7686813844277758 1106 12 0.27735518382095137 1106 13 0.3072486163566239 1106 14 0.7110390984254167 1106 15 0.9497979449243275 1106 16 0.1394384975801266 1106 17 0.32073713264563253 1106 18 0.9757604768147594 1106 19 0.016444970977381956 1106 20 0.5862723556102551 1106 21 0.17987931353836828 1106 22 0.4450177120933816 1106 23 0.2741963838115389 1106 24 0.1413850934804728 1106 25 1.1604285371147611 1106 26 -0.09651708807652426 1106 27 0.052366051338296415 1106 28 0.42602350730519944 1106 29 1.3195105557351674 1106 30 1.743035926974354 1106 31 1.0441060720658868 1106 32 1.1984281119574263 1106 33 1.3230043478535372 1106 34 2.033710741205482 1106 35 1.4145393515973872 1106 36 0.681365928600286 1106 37 1.0757315118564499 1106 38 0.9668097062845078 1106 39 -0.14160753312145147 1106 40 0.028108207554275566 1106 41 -0.1910627487231018 1106 42 -0.0019426229265420147 1106 43 -0.21006999318500164 1106 44 0.31839799100183086 1106 45 0.6329586234035283 1106 46 1.0878912480624763 1106 47 0.11181137577553812 1106 48 0.4177114586880677 1106 49 1.3206467732556 1106 50 0.7611682528811272 1107 1 0.8694239973527829 1107 2 0.3481487778841199 1107 3 0.9164099017758787 1107 4 -0.034475713016441034 1107 5 -0.5233978067444021 1107 6 -0.19643159866922133 1107 7 -0.14734550030904714 1107 8 0.344255539340633 1107 9 -0.18601616694168044 1107 10 0.7217595528790923 1107 11 0.270508683424886 1107 12 0.3486198447082529 1107 13 -0.24132354070609807 1107 14 -1.4610669699070655 1107 15 -1.2197584038773073 1107 16 -0.430338826914177 1107 17 -0.2912380500842673 1107 18 0.26676846593595727 1107 19 -0.30095261528812856 1107 20 -0.48601219308939037 1107 21 1.2917695056172391 1107 22 0.9525818800349057 1107 23 0.06018992452206007 1107 24 0.13785798146279404 1107 25 0.5943483059401906 1107 26 -0.3924970302828825 1107 27 -0.9060735203088228 1107 28 -0.6601145175120706 1107 29 -0.5597177291534976 1107 30 -0.2848410065629601 1107 31 0.09327316368580796 1107 32 -0.36918195093565487 1107 33 0.19420101622001695 1107 34 -0.029947256414779133 1107 35 0.587027877859498 1107 36 0.8281872782518214 1107 37 -0.39243948933341954 1107 38 -0.6935251568805434 1107 39 -0.06554090045104743 1107 40 -0.3714328429301723 1107 41 -0.2907992777049131 1107 42 0.5104660121236009 1107 43 0.02633150265045714 1107 44 -0.8340752369430966 1107 45 0.3653230633151381 1107 46 -0.4712197497277657 1107 47 -1.2851799551780552 1107 48 -1.0643555139449425 1107 49 0.07022182000045873 1107 50 -0.48237245764071446 1108 1 1.1667824879188196 1108 2 1.256073306186007 1108 3 0.5118815998890831 1108 4 1.4413733435873297 1108 5 1.519243702689561 1108 6 1.1698577095068785 1108 7 0.8688809147852936 1108 8 0.49205428147899966 1108 9 1.4021599336872512 1108 10 0.7976086094828645 1108 11 1.3399426456890353 1108 12 0.5765443730101403 1108 13 0.19364501275302004 1108 14 0.8596877470740658 1108 15 1.5110592061855854 1108 16 1.412051110192742 1108 17 0.6579443398528397 1108 18 1.1730577741120563 1108 19 2.168607133139542 1108 20 2.508704788042685 1108 21 1.5044739081329617 1108 22 1.1179906850663528 1108 23 0.6044666540818078 1108 24 1.3661598682552487 1108 25 2.384662771348996 1108 26 1.8549243533649182 1108 27 1.6579516369238831 1108 28 2.148816300097991 1108 29 2.7458168620414725 1108 30 2.0740269179653446 1108 31 1.425097063056878 1108 32 2.1550947786240933 1108 33 3.1903016451508357 1108 34 1.8765936240883647 1108 35 1.7144492615072977 1108 36 0.792086649321007 1108 37 1.1042900704150078 1108 38 1.755908805383605 1108 39 2.872446520932602 1108 40 1.585045144491211 1108 41 1.5133516656913137 1108 42 1.401660980677061 1108 43 1.6215516284366203 1108 44 1.2508304234342624 1108 45 1.0201658306107362 1108 46 0.6577110678822962 1108 47 1.4680275919917563 1108 48 0.3108646118412217 1108 49 0.6596557822646112 1108 50 0.5742313159441892 1109 1 0.1908685094241953 1109 2 0.5601593276913803 1109 3 0.813386976233172 1109 4 1.146534633909912 1109 5 1.6497813370981618 1109 6 1.766846956818707 1109 7 0.8875905922046492 1109 8 1.5576456793284592 1109 9 1.911407245515207 1109 10 0.9371785019559837 1109 11 0.5971469467643047 1109 12 1.2358992117198184 1109 13 2.009558991247644 1109 14 1.4854941986869683 1109 15 0.9927796362931147 1109 16 1.343448959655106 1109 17 0.9564389635087522 1109 18 0.4278964837894783 1109 19 1.2866716492685768 1109 20 -0.2345210184089268 1109 21 0.5180222952297342 1109 22 1.8308939108728062 1109 23 0.7444666540818083 1109 24 1.0640093306208414 1109 25 1.119931588553296 1109 26 0.5325587619670671 1109 27 1.8454785186443132 1109 28 0.39677328934530465 1109 29 0.2634512706436247 1109 30 1.4028441222664192 1109 31 0.17004329961601528 1109 32 -0.44834608159096234 1109 33 0.6090113225701899 1109 34 -0.1726536877395901 1109 35 0.04971807871159584 1109 36 0.5660651439446625 1109 37 0.4498814682644685 1109 38 0.31698407420081054 1109 39 1.6253497467390545 1109 40 0.74676557459874 1109 41 0.7303409130031397 1109 42 0.6780050666985673 1109 43 0.5260677574688764 1109 44 0.9701852621439393 1109 45 0.11220884136342235 1109 46 0.21147450874251206 1109 47 1.679640495217562 1109 48 1.3558108484003633 1109 49 1.0398708360280509 1109 50 0.38928507938505064 1110 1 0.4957276355981435 1110 2 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1.2918305678324664 2214 9 0.9163433788774071 2214 10 1.567228296283762 2214 11 1.965624972310959 2214 12 1.203121554218196 2214 13 1.2064414333346747 2214 14 -0.006084065006472628 2214 15 1.1450636804585166 2214 16 1.085384443526074 2214 17 1.0978548543942281 2214 18 0.38044032444762976 2214 19 0.5225668646831672 2214 20 0.973402774619867 2214 21 0.6385678678645057 2214 22 0.780406792448904 2214 23 1.9337283990482526 2214 24 1.3015066244073736 2214 25 0.7296739570313235 2214 26 0.5501816240584319 2214 27 1.4108822856934573 2214 28 2.4878319600532492 2214 29 0.9871144011913593 2214 30 1.2821253519698175 2214 31 0.9103767051150413 2214 32 1.5356764341050777 2214 33 1.813254665285065 2214 34 1.286839709099553 2214 35 1.3775812525587519 2214 36 1.938440116882525 2214 37 2.0699723970369313 2214 38 1.3505844205961353 2214 39 1.5735203464359557 2214 40 1.3017341825225337 2214 41 1.0829713524922084 2214 42 1.5334283184846669 2214 43 2.0038335076312492 2214 44 1.5897565979309078 2214 45 1.7808817142796407 2214 46 1.931760284884536 2214 47 2.485745712797126 2214 48 1.8572180794027418 2214 49 1.4812217777903385 2214 50 1.3147234859665602 2215 1 -0.3183389248847135 2215 2 -0.16361764304136628 2215 3 -0.14277623675550544 2215 4 -0.2588915570749215 2215 5 0.5937249388926524 2215 6 0.37056411127288413 2215 7 0.0634504512091354 2215 8 -0.4825594050552162 2215 9 -0.5555442605732352 2215 10 -0.29294326689682393 2215 11 0.19936869425415793 2215 12 -0.31325695149316957 2215 13 -0.6203064221255659 2215 14 -0.312497683389509 2215 15 -0.3330909041896888 2215 16 0.2112122580956104 2215 17 -0.8887223268138271 2215 18 -0.4223064642985399 2215 19 0.7266203781726546 2215 20 0.18923458936718873 2215 21 0.9549374842919001 2215 22 0.3981231353974799 2215 23 -0.9389770545274629 2215 24 -0.14620216264982844 2215 25 -0.9690237628673408 2215 26 -0.10264739056444228 2215 27 -0.7009004602063591 2215 28 -0.4837223312485861 2215 29 0.1103643234101277 2215 30 0.8753072711662249 2215 31 0.6879668202312708 2215 32 -0.5846844708240297 2215 33 -0.4376453747167126 2215 34 -0.3361657578100896 2215 35 -0.8604293256891715 2215 36 -1.1867654478092362 2215 37 -0.7161514306887433 2215 38 -0.47989693413074264 2215 39 -0.9782819558885905 2215 40 -1.1221513234999563 2215 41 -1.015610806714868 2215 42 -0.5363081142456743 2215 43 -1.1530179079872234 2215 44 -1.2425470600094464 2215 45 -1.167560438704939 2215 46 -1.1623772323384536 2215 47 -1.1785949245645355 2215 48 -1.4666629819777626 2215 49 -1.3364590080223664 2215 50 -0.5405589710315262 2216 1 0.06682338771431873 2216 2 0.361267580214637 2216 3 0.0757261806661802 2216 4 0.25965555217628733 2216 5 -0.007095765613097882 2216 6 0.15137100194041864 2216 7 0.08323674300615025 2216 8 0.3192526454871789 2216 9 -0.06393413584240193 2216 10 1.1058703681740738 2216 11 0.11327597902236874 2216 12 0.4312682993905099 2216 13 0.20722374485935902 2216 14 0.7431233298961502 2216 15 -0.053766969683533006 2216 16 -1.2029591147561334 2216 17 -0.5942437992064669 2216 18 0.5000516391427325 2216 19 -0.04992047422242152 2216 20 -0.0932584021413625 2216 21 0.17478065659921782 2216 22 0.7370090899743289 2216 23 0.8389451816891693 2216 24 0.47802080895054644 2216 25 -0.08335358856920294 2216 26 -0.03902247690093219 2216 27 0.18907638130016124 2216 28 0.19376517535361515 2216 29 0.3753056145977105 2216 30 1.1532498218508245 2216 31 0.8975715006846876 2216 32 0.6140722837365686 2216 33 0.6647801727581957 2216 34 0.13029505558120746 2216 35 -0.02473274258268532 2216 36 0.21854881701016637 2216 37 0.38940254485263637 2216 38 0.5970948994531362 2216 39 0.670115232589044 2216 40 0.46709013426626456 2216 41 0.7504477802107381 2216 42 0.811272432619802 2216 43 1.0112448799703628 2216 44 -0.009905772884756914 2216 45 0.2931310657845607 2216 46 -0.12040754152465638 2216 47 0.3433650153046379 2216 48 0.10665193290461517 2216 49 0.1849727556797447 2216 50 0.7132906206476655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 4.0 6 25.0 7 466.0 8 2745.0 9 6198.0 10 5945.0 11 4767.0 12 4346.0 13 4419.0 14 4337.0 15 4343.0 16 4030.0 17 3720.0 18 3396.0 19 3119.0 20 2702.0 21 2638.0 22 2387.0 23 2250.0 24 2166.0 25 1996.0 26 1947.0 27 1768.0 28 1729.0 29 1659.0 30 1500.0 31 1498.0 32 1349.0 33 1308.0 34 1330.0 35 1347.0 36 1346.0 37 1500.0 38 2206.0 39 7582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.742416178818523 32.18307610431081 8.910058541777541 27.164449175093136 2 18.28397082899241 32.23268768736845 30.164990538558033 19.318350945081114 3 18.25729228675908 34.69470193043626 25.991793519857133 21.05621226294753 4 16.57347563568331 28.701420188791566 27.62777447061825 27.09732970490688 5 24.04885671460918 33.13242125628514 27.397391332078964 15.421330697026715 6 26.953822603962923 38.28237945403521 13.744791223743515 21.019006718258353 7 26.305383110808744 32.0244068373161 15.024661961051109 26.64554809082405 8 24.34093035122034 29.980227910536613 17.618419933667827 28.06042180457522 9 25.573509088976294 23.13277346656745 13.970447539066653 37.32326990538961 10 22.214308493674924 27.714467949399385 25.973211438290633 24.098012118635058 11 32.094867542628734 23.05885104392567 19.72296636475741 25.123315048688184 12 23.946506782327678 25.53365650380576 22.4890504741251 28.030786239741463 13 28.354559564561054 20.241107307635065 22.020708863989114 29.383624263814767 14 32.740493478052876 21.65903026566171 18.07328818820628 27.527188068079134 15 26.387855335615416 26.402738502753387 17.96504581889312 29.24436034273808 16 29.79645282858177 22.245406349138758 19.99489002916942 27.963250793110056 17 35.42087470499437 22.443816044054174 17.012523122063232 25.122786128888226 18 29.516661343553714 20.75907590759076 18.79378260406686 30.930480144788675 19 29.02370455561945 20.991203969927376 21.73769514195046 28.247396332502717 20 33.986788834434265 20.858725761772853 20.008523332623053 25.145962071169826 21 27.567101043142856 22.92570137771574 18.167094650030364 31.340102929111037 22 30.72808099714979 19.914280852512018 17.460756370442848 31.896881779895352 23 37.467837625194036 21.713058455780722 16.987049992557466 23.832053926467776 24 28.55531194789161 28.482939185593565 16.634027969944018 26.32772089657081 25 33.979580984792086 22.19185366372434 16.6872274805913 27.14133787089227 26 32.750241920905154 26.168929912057763 17.105668924595115 23.975159242441965 27 29.740852199619304 27.162134858941506 18.751794468252534 24.345218473186655 28 34.01777929010442 24.293400820909806 18.555539014482893 23.13328087450288 29 31.20686538277487 24.278741346491277 20.612099492752854 23.902293777981008 30 33.64430308381291 22.82489601838161 19.44855170359654 24.08224919420894 31 32.69844308320572 21.49587374510805 21.384209630763994 24.42147354092224 32 30.26065317494922 22.56760924356343 21.57221400997522 25.59952357151213 33 34.60630423685554 21.644759230900117 21.08750212693551 22.66143440530883 34 30.18707392557457 22.0596211726419 23.063588118306445 24.689716783477085 35 33.142620143992005 22.761158316761136 20.85544436527602 23.24077717397084 36 34.38158034669786 20.151015633308518 22.993725406785067 22.473678613208552 37 31.168320200763493 20.951499877712912 24.666900607181972 23.213279314341616 38 32.77503057372255 19.252406019035465 23.998511192641036 23.974052214600945 39 30.208765380893126 20.518138021248763 26.288698167586595 22.98439843027151 40 25.600587809345317 31.97065212761426 22.529496954466076 19.89926310857435 41 30.37434861214506 17.894289056684038 26.19589492715091 25.535467404019997 42 25.02738516840549 20.824426506716012 30.628848546724946 23.519339778153547 43 25.559608247641936 18.18926190197684 28.843352226204022 27.407777624177204 44 26.424743758771747 17.747628971207416 31.059413941224 24.768213328796836 45 25.806005699459828 19.774786270256474 31.441665603334613 22.97754242694909 46 25.636582072573017 20.056986720818227 27.92243001584146 26.384001190767304 47 29.958003295943865 20.76338312689384 27.499867099037793 21.778746478124503 48 29.652114742594684 21.28245476003147 24.812341846173474 24.253088651200375 49 27.679435248088964 23.191826406829755 25.836974664838024 23.291763680243253 50 29.034556087187667 22.700691121743755 23.71185539606592 24.552897395002656 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 1.0 3 2.0 4 3.0 5 2.5 6 2.0 7 3.0 8 4.0 9 3.0 10 2.0 11 4.0 12 6.0 13 8.0 14 10.0 15 11.0 16 12.0 17 19.0 18 26.0 19 46.5 20 67.0 21 95.5 22 124.0 23 151.0 24 178.0 25 246.5 26 315.0 27 379.0 28 443.0 29 582.0 30 721.0 31 856.5 32 992.0 33 1245.5 34 1499.0 35 1823.5 36 2148.0 37 2393.0 38 2638.0 39 2999.0 40 3360.0 41 3626.5 42 3893.0 43 4241.5 44 4590.0 45 4925.0 46 5260.0 47 5587.0 48 5914.0 49 6173.0 50 6432.0 51 6702.5 52 6973.0 53 6760.5 54 6548.0 55 6443.0 56 6338.0 57 6041.0 58 5744.0 59 5339.0 60 4934.0 61 4701.5 62 4469.0 63 4076.5 64 3684.0 65 3359.0 66 3034.0 67 2839.5 68 2645.0 69 2434.0 70 2223.0 71 2068.5 72 1914.0 73 1817.0 74 1720.0 75 1580.0 76 1440.0 77 1259.0 78 1078.0 79 992.0 80 906.0 81 804.0 82 702.0 83 564.0 84 426.0 85 348.0 86 270.0 87 213.0 88 156.0 89 133.0 90 110.0 91 81.0 92 52.0 93 36.5 94 21.0 95 19.5 96 18.0 97 15.0 98 12.0 99 13.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12968789863083596 2 0.006378093375287014 3 0.0 4 0.0 5 0.0010630155625478356 6 0.0 7 0.0 8 0.0 9 0.0021260311250956712 10 0.0021260311250956712 11 0.0042520622501913425 12 0.0042520622501913425 13 0.006378093375287014 14 0.0053150778127391786 15 0.006378093375287014 16 0.14669614763160133 17 0.006378093375287014 18 0.15094820988179267 19 0.17646058338294074 20 0.22535929926014117 21 0.2349264393230717 22 0.04677268475210477 23 0.019134280125861044 24 0.12118377413045327 25 0.0446466536270091 26 0.035079513564078575 27 0.035079513564078575 28 0.03189046687643507 29 0.03720554468917425 30 0.06909601156560932 31 0.04252062250191343 32 0.04145760693936559 33 0.04252062250191343 34 0.047835700314652604 35 0.04145760693936559 36 0.0446466536270091 37 0.032953482438982905 38 0.03933157581426992 39 0.045709669189556934 40 0.17433455225784505 41 0.0446466536270091 42 0.045709669189556934 43 0.035079513564078575 44 0.021260311250956714 45 0.0297644357513394 46 0.015945233438217535 47 0.01807126456331321 48 0.019134280125861044 49 0.013819202313121863 50 0.023386342376052385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 94072.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.28952291861553 #Duplication Level Percentage of deduplicated Percentage of total 1 96.86050051756683 84.54906879836722 2 0.9255312671253729 1.61578365507271 3 0.43597393898800463 1.1416787141763756 4 0.30201546611459534 1.054511438047453 5 0.2666991414479693 1.1640020409898801 6 0.21798696949400231 1.1416787141763756 7 0.21189794799975642 1.294752955183264 8 0.17292821043658285 1.2075856790543413 9 0.14978992875844852 1.176758227740454 >10 0.4493697862753455 5.1216089803554725 >50 0.006089021494245874 0.3954417892677949 >100 0.001217804298849175 0.1371290075686708 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 129 0.1371290075686708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0148822178756697 0.0 2 0.0 0.0 0.0 0.02232332681350455 0.0 3 0.0 0.0 0.0 0.02232332681350455 0.0 4 0.0 0.0 0.0 0.02232332681350455 0.0 5 0.0 0.0 0.0 0.028701420188791564 0.0 6 0.0 0.0 0.0 0.028701420188791564 0.0 7 0.0 0.0 0.0 0.028701420188791564 0.0 8 0.0 0.0 0.0 0.0297644357513394 0.0 9 0.0 0.0 0.0 0.032953482438982905 0.0 10 0.0 0.0 0.0 0.03401649800153074 0.0 11 0.0 0.0 0.0 0.03614252912662641 0.0 12 0.0 0.0 0.0 0.03614252912662641 0.0 13 0.0 0.0 0.0 0.03826856025172209 0.0 14 0.0 0.0 0.0 0.03826856025172209 0.0 15 0.0 0.0 0.0 0.04252062250191343 0.0 16 0.0 0.0 0.0 0.04252062250191343 0.0 17 0.0 0.0 0.0 0.045709669189556934 0.0 18 0.0 0.0 0.0 0.049961731439748275 0.0 19 0.0 0.0 0.0 0.05846585594013096 0.0 20 0.0 0.0 0.0 0.06590696487796581 0.0 21 0.0 0.0 0.0 0.07015902712815715 0.0 22 0.0 0.0 0.0 0.08291521387873119 0.0 23 0.0 0.0 0.0 0.08504124500382686 0.0 24 0.0 0.0 0.0 0.08716727612892253 0.0 25 0.0 0.0 0.0 0.0892933072540182 0.0 26 0.0 0.0 0.0 0.09141933837911387 0.0 27 0.0 0.0 0.0 0.09567140062930521 0.0 28 0.0 0.0 0.0 0.13181392975593162 0.0 29 0.0 0.0 0.0 0.15094820988179267 0.0 30 0.0 0.0 0.0 0.17752359894548855 0.0 31 0.0 0.0 0.0 0.2083510502593758 0.0 32 0.0 0.0 0.0 0.2413045326983587 0.0 33 0.0 0.0 0.0 0.29339229526320265 0.0 34 0.0 0.0 0.0 0.37205544689174247 0.0 35 0.0 0.0 0.0 0.4432774895824475 0.0 36 0.0 0.0 0.0 0.5931626839016924 0.0 37 0.0 0.0 0.0 0.7855685007228506 0.0 38 0.0 0.0 0.0 1.0066757377328004 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGCGC 20 7.860696E-4 43.953217 8 TGTTGGC 25 0.0023174197 35.275093 20 CCCTTGC 25 0.0023356862 35.218746 37 TTATGCT 25 0.0023540666 35.16257 9 TATGCTT 35 3.2054968E-4 31.411854 10 CTTTACA 115 0.0 30.64131 1 CGGTGGT 115 0.0 30.57615 43 CTCTACA 30 0.005688716 29.36459 1 TATCACA 30 0.005748269 29.302145 3 TATATTA 30 0.005748269 29.302145 5 ATAGACA 30 0.005748269 29.302145 4 TTATCAC 30 0.005748269 29.302145 2 TATTATG 30 0.005748269 29.302145 7 ATTATGC 30 0.005748269 29.302145 8 ATATTAT 30 0.005748269 29.302145 6 TTACACA 135 0.0 29.302143 3 GGTGGTC 115 0.0 28.665142 44 CTATACA 70 3.480818E-8 28.315855 1 CTTATAC 585 0.0 27.858713 1 TTTACAC 135 0.0 27.674248 2 >>END_MODULE