FastQCFastQC Report
Wed 25 May 2016
SRR1780484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28641
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGATC150.00220403769.989518
ATCAAAA207.803175E-569.9895112
CATTGGG200.00688735252.49213439
TTGTAGA200.00688735252.4921343
CCCGACG200.00688735252.49213461
GGGCATT305.7875185E-446.6596736
ACCACCC808.3927756E-526.2460672
CGACGAA750.001791457823.32983870
ACCACAC1250.001708263516.7974822
CCACACC1553.889927E-415.8040833
TGCCGAC1500.00575626913.99790268
CTGCCGA1600.00882421713.12303467
Produced by FastQC (version 0.11.5)