##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780484_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28641 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.481512517021052 32.0 21.0 34.0 12.0 34.0 2 27.437554554659403 32.0 23.0 34.0 11.0 34.0 3 27.630564575259243 33.0 23.0 34.0 11.0 34.0 4 28.055200586571697 34.0 23.0 34.0 11.0 34.0 5 27.987151286617088 34.0 23.0 34.0 11.0 34.0 6 29.679305890157465 34.0 23.0 38.0 11.0 38.0 7 29.554694319332427 34.0 23.0 37.0 11.0 38.0 8 28.370866939003527 34.0 21.0 37.0 11.0 38.0 9 29.247232987675012 34.0 21.0 37.0 12.0 38.0 10-11 29.23129429838344 34.0 21.5 37.5 11.0 38.0 12-13 29.57531161621452 35.0 23.0 38.0 11.0 38.0 14-15 29.238818477008486 34.0 21.0 38.0 11.0 38.0 16-17 28.954610523375578 34.0 21.0 38.0 11.0 38.0 18-19 29.061363080898012 34.0 21.5 38.0 11.0 38.0 20-21 29.15139136203345 34.0 22.0 38.0 11.0 38.0 22-23 28.95860828881673 34.0 21.0 38.0 11.0 38.0 24-25 29.34007192486296 35.5 21.5 38.0 11.0 38.0 26-27 29.404437694214586 35.5 21.5 38.0 11.0 38.0 28-29 29.375213854264864 36.0 21.0 38.0 11.0 38.0 30-31 29.264707936175412 36.0 21.0 38.0 11.0 38.0 32-33 29.126584267309102 35.0 21.0 38.0 11.0 38.0 34-35 29.11176285744213 34.5 21.0 38.0 11.0 38.0 36-37 28.999319157850636 34.5 21.0 38.0 11.0 38.0 38-39 29.289043678642507 36.0 21.0 38.0 11.0 38.0 40-41 29.007070283858802 34.5 21.0 38.0 11.0 38.0 42-43 29.22942634684543 35.0 21.0 38.0 11.0 38.0 44-45 28.389651199329634 34.0 21.0 37.5 11.0 38.0 46-47 29.0215600013966 34.0 21.0 38.0 11.0 38.0 48-49 28.9390384413952 34.0 21.0 38.0 11.0 38.0 50-51 28.816347194581194 34.0 21.0 37.5 11.0 38.0 52-53 28.751108550679096 34.0 21.0 37.5 11.0 38.0 54-55 28.567839810062498 34.0 21.0 37.0 11.0 38.0 56-57 28.586274920568414 34.0 21.0 37.0 11.0 38.0 58-59 28.415313711113438 34.0 21.0 37.0 11.0 38.0 60-61 28.491637861806502 34.0 21.0 37.0 11.0 38.0 62-63 28.462536224293846 34.0 20.5 37.0 11.0 38.0 64-65 28.521629831360634 34.0 20.5 37.0 11.0 38.0 66-67 28.590115568590484 34.0 21.0 37.5 11.0 38.0 68-69 28.22663314828393 33.0 20.0 37.0 11.0 38.0 70-71 28.23841695471527 33.0 20.5 37.0 11.0 38.0 72-73 28.06200900806536 33.0 19.0 37.0 11.0 38.0 74-75 27.62438462344192 32.0 19.0 37.0 11.0 38.0 76 26.537481233197166 31.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.5186323827546744 1101 2 -1.6506325997829272 1101 3 -0.15167105219931187 1101 4 -1.7518318310834644 1101 5 -0.9793831541299909 1101 6 -2.4589763335712753 1101 7 -0.6972405863239572 1101 8 -1.1983691220198516 1101 9 -0.8918532509010078 1101 10-11 -2.3909992230331554 1101 12-13 -2.672741493440153 1101 14-15 -2.1771277514005085 1101 16-17 -2.2995669498499254 1101 18-19 -1.0257618793747696 1101 20-21 -0.9415341413155858 1101 22-23 -0.9716034712268815 1101 24-25 -2.4790143324234393 1101 26-27 -2.4829631563511363 1101 28-29 -2.0337922358581544 1101 30-31 -1.5165348196194515 1101 32-33 -1.9192637533578214 1101 34-35 -2.529074749215358 1101 36-37 -1.1038731779934672 1101 38-39 -2.211550794633915 1101 40-41 -1.15426232825563 1101 42-43 -2.5665049484047913 1101 44-45 -0.998213785024948 1101 46-47 -1.6431417736498766 1101 48-49 -2.1916911706989417 1101 50-51 -3.554673609764116 1101 52-53 -1.926659825497186 1101 54-55 -2.9552186940107674 1101 56-57 -1.8834215978028368 1101 58-59 -1.348910469829331 1101 60-61 -1.0373778392740505 1101 62-63 -1.8780754326794415 1101 64-65 0.42939756094695625 1101 66-67 -1.7345168716346393 1101 68-69 -1.6721023611175028 1101 70-71 -1.3534987912460927 1101 72-73 0.18189706408269046 1101 74-75 -0.8864694959353656 1101 76 -0.7621103350577805 1102 1 0.002639448861984306 1102 2 -0.7215387051717919 1102 3 0.693828276058639 1102 4 0.6392713186403718 1102 5 0.6814617480942218 1102 6 1.746576734050759 1102 7 1.1718443517264987 1102 8 1.7092290269576083 1102 9 0.06903643412660543 1102 10-11 -0.32136196374072057 1102 12-13 -3.5582467927385544 1102 14-15 -1.997847261775192 1102 16-17 -2.4737720550895155 1102 18-19 -1.1845169174401526 1102 20-21 -0.1569394256863852 1102 22-23 -1.29306936165829 1102 24-25 -0.5291710722353535 1102 26-27 -4.064467858545495 1102 28-29 1.231919064335905 1102 30-31 -0.2576155779415892 1102 32-33 -6.007187324040014 1102 34-35 -0.5159384602169972 1102 36-37 0.9184435857026045 1102 38-39 1.0467713429985679 1102 40-41 1.0559929337237683 1102 42-43 0.44043638612274805 1102 44-45 -4.039115412133473 1102 46-47 -0.18098324252582998 1102 48-49 -0.4711358639367411 1102 50-51 -4.943686895329126 1102 52-53 -1.0118217899694955 1102 54-55 -0.08740260205674133 1102 56-57 -0.7316078942649966 1102 58-59 1.2354304765805786 1102 60-61 0.7120623756834057 1102 62-63 -4.450847488210119 1102 64-65 -0.49581515736921133 1102 66-67 -0.31393170967016815 1102 68-69 -7.112018766551149 1102 70-71 -0.8682024783635711 1102 72-73 -0.8896061453515536 1102 74-75 -4.575452926298105 1102 76 -1.9734851671222664 1103 1 0.8709780068557151 1103 2 -0.8786268277771541 1103 3 0.5395266389983817 1103 4 -2.046204125455759 1103 5 -0.5522547270015643 1103 6 -1.6797555543504963 1103 7 -0.30185819094155875 1103 8 -0.09014401379474535 1103 9 -2.2785776376253963 1103 10-11 -0.9775793096132404 1103 12-13 -3.4180517387503997 1103 14-15 -1.4931450674178244 1103 16-17 -3.134343284626258 1103 18-19 -1.4882438418567325 1103 20-21 -1.6024288022102446 1103 22-23 -1.1021951018185128 1103 24-25 -1.751741605150709 1103 26-27 -1.8718520452400256 1103 28-29 -0.9544271564930753 1103 30-31 -1.1846444877291233 1103 32-33 -3.484198818292885 1103 34-35 -1.1683243884649954 1103 36-37 -0.2597173338376244 1103 38-39 -1.6242492073323263 1103 40-41 -1.1831223571156606 1103 42-43 -0.9965193784192223 1103 44-45 -3.83082561763678 1103 46-47 -0.8213524518605553 1103 48-49 -0.9218499008576728 1103 50-51 -1.7198972749877832 1103 52-53 -2.285245684083044 1103 54-55 -1.4501681889602622 1103 56-57 0.36261014822891013 1103 58-59 -0.9686795895984517 1103 60-61 -0.7531065550027698 1103 62-63 -0.6753337299377371 1103 64-65 -0.2250035934542005 1103 66-67 -1.0072441443619127 1103 68-69 -2.030688219703361 1103 70-71 -0.683946121693424 1103 72-73 -0.7957364135507881 1103 74-75 -1.6750697845356513 1103 76 -1.366727939675382 1104 1 -2.3009587520375625 1104 2 -0.941428760144305 1104 3 -0.39800080939863136 1104 4 -0.8601039937034543 1104 5 -1.0365542439097766 1104 6 -1.9119689930856723 1104 7 0.3833635921063099 1104 8 -0.05226522591595639 1104 9 -0.08863473029118296 1104 10-11 -0.034068110667256235 1104 12-13 -0.6516375973362578 1104 14-15 0.9684820735571407 1104 16-17 -0.23145571326042713 1104 18-19 0.6702432024998757 1104 20-21 0.9322659716149637 1104 22-23 0.526208499411176 1104 24-25 -0.14255188183055267 1104 26-27 -0.440967108920308 1104 28-29 -0.42319088069158184 1104 30-31 0.043671278630121435 1104 32-33 -0.4787640356841898 1104 34-35 -0.03218033927747044 1104 36-37 1.1641957096406337 1104 38-39 0.16302571581216085 1104 40-41 0.3858280161825398 1104 42-43 -0.04850664236300872 1104 44-45 -2.6594927234778005 1104 46-47 0.1482896210423874 1104 48-49 0.4010530416104885 1104 50-51 0.16525863190725332 1104 52-53 0.9606919531589426 1104 54-55 0.7295639146848849 1104 56-57 -0.06481629006709255 1104 58-59 0.8359427204586396 1104 60-61 0.1697085525949511 1104 62-63 0.2007642059428072 1104 64-65 0.12787299855282797 1104 66-67 -0.042817266891560735 1104 68-69 -4.312418566651097 1104 70-71 -0.012942608298608604 1104 72-73 0.40655827243955045 1104 74-75 -1.4534514270477317 1104 76 1.035885147720311 1105 1 -0.8103715714010775 1105 2 -1.664422933160889 1105 3 -0.9442805046025775 1105 4 -1.3801622635376098 1105 5 -0.6496201336453211 1105 6 0.40459308669917604 1105 7 1.088540642756243 1105 8 0.635879103980951 1105 9 0.5946911325505901 1105 10-11 -0.2918560981168312 1105 12-13 -1.2766375973362578 1105 14-15 -1.3142947081550957 1105 16-17 0.5374259541532886 1105 18-19 0.715234237910007 1105 20-21 -0.3897869194652692 1105 22-23 -0.41938764581517773 1105 24-25 -0.35249137084893434 1105 26-27 -0.0518478798750337 1105 28-29 -0.3458152643760286 1105 30-31 -0.15517453042411233 1105 32-33 0.3096156146968063 1105 34-35 1.5047985939363393 1105 36-37 0.7609012228634064 1105 38-39 0.4837899470985789 1105 40-41 0.7228965952950439 1105 42-43 0.9356372393044055 1105 44-45 2.3562513374814102 1105 46-47 1.0467096120777981 1105 48-49 0.5802327816373811 1105 50-51 0.02070462025328723 1105 52-53 -0.6361256174372016 1105 54-55 -0.5634661166911812 1105 56-57 -0.37398258320918387 1105 58-59 -1.1924638236919627 1105 60-61 0.3894530617836516 1105 62-63 0.5171581996675947 1105 64-65 0.1357170146890887 1105 66-67 0.3034400168377118 1105 68-69 0.7088723342901844 1105 70-71 -0.06028684854961597 1105 72-73 0.5536335749944605 1105 74-75 1.5322612578469332 1105 76 0.15769151257912029 1106 1 -0.8710674476897395 1106 2 -1.3925157166660433 1106 3 -1.195826896355154 1106 4 -2.299234428486063 1106 5 -2.1175325047793443 1106 6 -3.008164645259587 1106 7 -1.1362016252849934 1106 8 -0.5397652259159571 1106 9 -0.9071129911607478 1106 10-11 0.008594932811000433 1106 12-13 -0.8953875973362564 1106 14-15 -0.49238749166025286 1106 16-17 -0.9026513654343411 1106 18-19 -1.138180710543601 1106 20-21 -0.4343101153415603 1106 22-23 -1.75585671798013 1106 24-25 -0.9966279687870774 1106 26-27 -1.3608040654420464 1106 28-29 -2.6408539241698463 1106 30-31 -3.0639374170220535 1106 32-33 -1.4154488182928837 1106 34-35 -2.602289034929644 1106 36-37 -1.722217333837623 1106 38-39 -1.4391481972313152 1106 40-41 -1.1375415490348537 1106 42-43 -2.068615338015178 1106 44-45 -0.0978079408691066 1106 46-47 -1.1661125528706542 1106 48-49 -1.5166100018677717 1106 50-51 -0.9890891941797015 1106 52-53 -0.9425689164062767 1106 54-55 -1.0802186940107674 1106 56-57 -1.4376423770236144 1106 58-59 -1.4651442360631002 1106 60-61 -0.5234979691441808 1106 62-63 -1.5924423157963261 1106 64-65 -1.1324530884036967 1106 66-67 0.18082403745626863 1106 68-69 -0.18687508839023081 1106 70-71 -0.38142086916817064 1106 72-73 -0.8339308579952309 1106 74-75 -3.9806253400912084 1106 76 -2.0385713740188187 1107 1 -0.562734114356406 1107 2 -0.7154323833327112 1107 3 0.22292310364484536 1107 4 1.2549322381806043 1107 5 -0.694615838112675 1107 6 1.437668688073746 1107 7 0.13879837471500522 1107 8 0.9060681074173758 1107 9 -2.0696129911607493 1107 10-11 0.066928266144334 1107 12-13 -0.9797625973362578 1107 14-15 -0.69863749166025 1107 16-17 -0.2932763654343411 1107 18-19 -0.5183890438769332 1107 20-21 -0.8895184486748953 1107 22-23 -1.2808567179801287 1107 24-25 -1.2195446354537438 1107 26-27 -1.4649707321087142 1107 28-29 -2.4877289241698435 1107 30-31 -2.4941457503553863 1107 32-33 -0.41649048495954943 1107 34-35 -0.8095807015963068 1107 36-37 -0.6503423338376244 1107 38-39 -1.1766481972313159 1107 40-41 -1.8229582157015152 1107 42-43 -1.2686153380151808 1107 44-45 -1.1436412742024409 1107 46-47 -0.6098625528706556 1107 48-49 -0.5593183352011053 1107 50-51 -0.20158919417970012 1107 52-53 -0.7311105830729403 1107 54-55 -1.0333436940107674 1107 56-57 -0.8449340436902801 1107 58-59 -0.6724359027297666 1107 60-61 -1.6724563024775172 1107 62-63 -1.063275649129661 1107 64-65 -0.10536975507036317 1107 66-67 -0.9504259625437328 1107 68-69 -0.4045834217235651 1107 70-71 -1.202254202501507 1107 72-73 -1.240180857995231 1107 74-75 -1.5587503400912084 1107 76 -2.82190470735215 1108 1 -1.2124334270711827 1108 2 -0.4788559228516114 1108 3 -0.2741774118190712 1108 4 -0.46263649034173326 1108 5 -0.6186922985937748 1108 6 1.280881746492991 1108 7 0.8926643540964498 1108 8 -0.8074198650912159 1108 9 0.5843818542000747 1108 10-11 0.6308243142543013 1108 12-13 0.5017129181276623 1108 14-15 1.3094166320510894 1108 16-17 -0.3130895097642359 1108 18-19 -0.31053895796627984 1108 20-21 0.09474916300895586 1108 22-23 -0.6461917695265171 1108 24-25 -0.4246563193025388 1108 26-27 0.0821727386816633 1108 28-29 0.716040405727064 1108 30-31 0.36028938710165903 1108 32-33 0.8663166456246394 1108 34-35 0.1852109650703575 1108 36-37 -0.5741503235283467 1108 38-39 1.4683260295728076 1108 40-41 2.1713502035424703 1108 42-43 1.6985238372425542 1108 44-45 0.253158553976256 1108 46-47 0.8559879625932645 1108 48-49 -0.115643507022412 1108 50-51 -0.48960465809722464 1108 52-53 1.049441392872076 1108 54-55 0.7509668729995411 1108 56-57 1.3631308188526745 1108 58-59 -0.47596897833114227 1108 60-61 0.8069788349795282 1108 62-63 0.46045716873975806 1108 64-65 -0.40036545953771707 1108 66-67 -0.27387957079115566 1108 68-69 1.2861919219190483 1108 70-71 1.9861049040277052 1108 72-73 0.7959016162315713 1108 74-75 1.1714365155788968 1108 76 -0.27529817814252766 1109 1 0.33950947538718523 1109 2 -0.4268563760067039 1109 3 0.28988738935913005 1109 4 0.09032601107437443 1109 5 0.12051694577010608 1109 6 0.2623023877074466 1109 7 -0.5054323945157648 1109 8 0.0933391696884378 1109 9 0.6645903055425464 1109 10-11 0.8857240536901223 1109 12-13 0.3497360290373699 1109 14-15 -0.2910138652866223 1109 16-17 -0.9898766401596149 1109 18-19 -0.7360515896644806 1109 20-21 0.21630801652657183 1109 22-23 0.908016908393499 1109 24-25 0.9945121411030335 1109 26-27 -0.3371090104969916 1109 28-29 -0.21111491318083253 1109 30-31 -0.4930582961429302 1109 32-33 0.44437261027854547 1109 34-35 0.39400217386156555 1109 36-37 0.152370578250288 1109 38-39 0.4627748796917608 1109 40-41 -0.23767891167221578 1109 42-43 0.3295989476991039 1109 44-45 -0.7969150837262511 1109 46-47 -1.114670245178349 1109 48-49 -1.2591237381315104 1109 50-51 0.8773943223038181 1109 52-53 1.5868266879893298 1109 54-55 -0.16950440829648272 1109 56-57 1.0021927878115484 1109 58-59 0.7255151045962442 1109 60-61 0.9865981847019718 1109 62-63 -0.6348186894226977 1109 64-65 -0.17599704444765152 1109 66-67 0.27347513635736576 1109 68-69 0.029196340181201208 1109 70-71 0.9477000099527082 1109 72-73 -1.4214995393139098 1109 74-75 1.763880154414288 1109 76 0.2209066479592039 1110 1 -0.03912300324529383 1110 2 -0.6675157166660419 1110 3 -0.7402713407995982 1110 4 -1.3936788729305079 1110 5 -0.7300325047793415 1110 6 -1.2928868674818084 1110 7 0.8193539302705624 1110 8 1.1532903296395993 1110 9 0.723442564394805 1110 10-11 -1.1407106227445531 1110 12-13 -2.009970930669592 1110 14-15 -0.8118319361046957 1110 16-17 -2.0929291432121175 1110 18-19 -1.645819599432489 1110 20-21 -1.3822267820082281 1110 22-23 -0.951690051313463 1110 24-25 -1.804266857675966 1110 26-27 -1.2809429543309356 1110 28-29 -2.155437257503177 1110 30-31 -1.0139374170220492 1110 32-33 -0.8175321516262208 1110 34-35 -0.4647890349296411 1110 36-37 -1.2430506671709587 1110 38-39 -2.208592641675761 1110 40-41 -0.647263771257073 1110 42-43 -1.061670893570735 1110 44-45 -0.6596134964246616 1110 46-47 -0.8612514417595456 1110 48-49 -0.39508222408999316 1110 50-51 -1.32797808306859 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2116 48-49 1.8478296533046397 2116 50-51 3.0074625299582287 2116 52-53 1.6289828077316528 2116 54-55 1.380988202540955 2116 56-57 2.182185209183281 2116 58-59 1.8762350742817269 2116 60-61 -0.5206962450062527 2116 62-63 2.687083546272639 2116 64-65 2.288667601251479 2116 66-67 1.1228498995252316 2116 68-69 2.0747628426442546 2116 70-71 -0.017627765719893773 2116 72-73 1.6649915557978723 2116 74-75 -0.5668322366429308 2116 76 -0.5596920636739888 2117 1 1.8779710138487218 2117 2 1.0209458217954968 2117 3 3.2734038728756154 2117 4 2.0546117253600897 2117 5 1.4776598029129637 2117 6 0.4985661239711838 2117 7 -1.0054323945157648 2117 8 -0.879188302839033 2117 9 3.1371177780700172 2117 10-11 0.4379218558879252 2117 12-13 2.559900864202202 2117 14-15 0.7749202006474434 2117 16-17 1.9194640191810421 2117 18-19 1.2488385202256325 2117 20-21 2.4209783461969003 2117 22-23 2.0604894358660246 2117 24-25 3.009622031212924 2117 26-27 2.761792088404107 2117 28-29 3.6625114604455433 2117 30-31 3.1649087368241027 2117 32-33 3.8715704124763413 2117 34-35 1.877518657378051 2117 36-37 1.6922057430854487 2117 38-39 2.3377748796917572 2117 40-41 1.7403430663497623 2117 42-43 1.3131154312155857 2117 44-45 2.855557443746278 2117 46-47 2.2026374471293444 2117 48-49 2.0829092289014604 2117 50-51 3.93783388274338 2117 52-53 3.020892622055264 2117 54-55 2.448627459835386 2117 56-57 2.3662037768225375 2117 58-59 1.0867788408599814 2117 60-61 2.9481366462404317 2117 62-63 1.8638076842036746 2117 64-65 2.8569699885193813 2117 66-67 2.684189422071654 2117 68-69 3.408317219302077 2117 70-71 3.2512714385241352 2117 72-73 2.4328960650816924 2117 74-75 2.2693746599087916 2117 76 3.987390164442722 2118 1 0.9364325523102615 2118 2 2.672484283333958 2118 3 0.7641731036448469 2118 4 1.8707655715139353 2118 5 2.1099674952206584 2118 6 2.829335354740415 2118 7 4.703798374715003 2118 8 0.23773477408404275 2118 9 2.5278870088392473 2118 10-11 2.1948449328110016 2118 12-13 3.403362402663742 2118 14-15 1.3526125083397496 2118 16-17 2.31484863456566 2118 18-19 3.2430692894563933 2118 20-21 1.4944398846584406 2118 22-23 2.241643282019872 2118 24-25 2.634622031212924 2118 26-27 4.177945934557954 2118 28-29 2.387896075830156 2118 30-31 3.1260625829779443 2118 32-33 2.845801181707113 2118 34-35 4.305210965070362 2118 36-37 2.630282666162376 2118 38-39 3.2158518027686824 2118 40-41 2.17495845096515 2118 42-43 1.9438846619848178 2118 44-45 2.7259420591308974 2118 46-47 2.3976374471293447 2118 48-49 2.307139998132225 2118 50-51 3.630910805820296 2118 52-53 2.4024310835937257 2118 54-55 3.7947813059892326 2118 56-57 3.1873576229763856 2118 58-59 4.1948557639369035 2118 60-61 1.0927520308558165 2118 62-63 3.178807684203676 2118 64-65 2.900046911596302 2118 66-67 1.5045740374562655 2118 68-69 3.7606249116097743 2118 70-71 0.6285791308318309 2118 72-73 0.6898191420047688 2118 74-75 2.7993746599087928 2118 76 1.7789286259811803 2119 1 1.2414325523102612 2119 2 3.4824842833339567 2119 3 1.029173103644844 2119 4 1.6007655715139357 2119 5 2.169967495220657 2119 6 1.2293353547404138 2119 7 -0.4612016252849962 2119 8 0.8727347740840443 2119 9 2.06788700883925 2119 10-11 3.452344932811002 2119 12-13 3.4358624026637408 2119 14-15 1.58261250833975 2119 16-17 2.8723486345656575 2119 18-19 1.9055692894564018 2119 20-21 1.2719398846584404 2119 22-23 3.2566432820198727 2119 24-25 3.084622031212927 2119 26-27 3.1829459345579565 2119 28-29 3.5278960758301565 2119 30-31 2.898562582977952 2119 32-33 2.9033011817071177 2119 34-35 3.660210965070359 2119 36-37 2.452782666162374 2119 38-39 3.448351802768684 2119 40-41 2.9624584509651477 2119 42-43 2.856384661984819 2119 44-45 2.6959420591308962 2119 46-47 3.5776374471293444 2119 48-49 3.414639998132227 2119 50-51 4.098410805820297 2119 52-53 0.3699310835937233 2119 54-55 1.3822813059892312 2119 56-57 3.6748576229763827 2119 58-59 4.322355763936901 2119 60-61 4.857752030855817 2119 62-63 3.276307684203676 2119 64-65 3.0425469115963075 2119 66-67 2.4745740374562644 2119 68-69 4.538124911609771 2119 70-71 1.0560791308318258 2119 72-73 -1.5651808579952302 2119 74-75 1.531874659908791 2119 76 3.5489286259811834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 8.0 13 62.0 14 283.0 15 652.0 16 1033.0 17 1375.0 18 1504.0 19 1371.0 20 1110.0 21 919.0 22 734.0 23 525.0 24 475.0 25 469.0 26 507.0 27 527.0 28 584.0 29 710.0 30 862.0 31 1035.0 32 1201.0 33 1622.0 34 1960.0 35 2748.0 36 3639.0 37 2726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.183767443495377 22.970930436922213 9.705086294773103 39.14021582480931 2 15.208966167382423 32.24398589434727 28.155441499947624 24.391606438322686 3 14.241821165462099 25.379700429454278 25.50888586292378 34.869592542159836 4 10.076463810621137 21.074683146538177 29.167975978492372 39.680877064348316 5 9.772703467057713 41.15778080374289 28.399846374079118 20.66966935512028 6 19.779337313641285 33.34031632973709 15.247372647603086 31.632973709018543 7 18.89598826856604 37.59994413602877 17.76823434935931 25.735833246045882 8 16.539226982298104 34.108445934150346 15.865367829335566 33.48695925421599 9 18.445585000523725 23.365105966970425 16.734750881603297 41.45455815090256 10-11 18.538109702873502 27.76439370133724 24.522537620893125 29.174958974896125 12-13 17.907894277434448 25.29241297440732 24.64823155616075 32.15146119199749 14-15 18.913445759575435 27.008484340630563 21.78520303062044 32.292866869173565 16-17 20.503823190531058 28.738521699661323 22.953109179148772 27.804545930658847 18-19 18.82964980273035 27.474599350581336 22.89375370971684 30.801997136971476 20-21 17.63032017038511 27.638699766069617 23.946440417583183 30.784539645962084 22-23 21.28591878775182 27.245906218358297 22.750602283439825 28.717572710450057 24-25 19.648406131070843 30.50521978981181 22.062777137669773 27.783596941447573 26-27 17.59715093746727 30.887538842917493 24.505080129883734 27.0102300897315 28-29 19.54366118501449 28.29510142802276 26.64187702943333 25.519360357529415 30-31 19.87709926329388 27.33319367340526 26.86533291435355 25.924374148947315 32-33 17.01057923955169 30.569812506546555 25.954051883663283 26.46555637023847 34-35 18.07024894382179 29.758039174609824 25.29066722530638 26.881044656262006 36-37 18.805209315317203 27.81676617436542 26.30320170385112 27.074822806466255 38-39 18.309416570650466 26.538877832477915 27.92325686952271 27.2284487273489 40-41 17.401627038162076 26.36954016968681 27.3349394225062 28.893893369644914 42-43 16.59858245173004 25.830103697496597 28.651234244614365 28.920079606159003 44-45 16.521769491288712 26.613945043818305 29.30938165566845 27.55490380922454 46-47 16.764428616319265 28.090848783212873 27.519988827205754 27.624733773262104 48-49 16.415278796131417 28.623302258999338 26.05705108061869 28.904367864250553 50-51 16.066128975943577 28.319541915435913 25.79169721727593 29.822631891344574 52-53 15.477811528927063 27.937222862330223 26.568555567193886 30.01641004154883 54-55 15.610488460598443 28.18861073286547 25.77947697356936 30.421423832966727 56-57 17.366712056143292 27.720749973813763 24.971195139834503 29.941342830208445 58-59 16.621277190042246 27.34715966621277 25.433818651583394 30.59774449216159 60-61 15.94741803707971 26.496979854055375 25.73408749694494 31.821514611919977 62-63 16.867427813274677 27.607276282252712 24.662197548968262 30.86309835550435 64-65 17.39289829265738 27.621242275060226 23.05087112880137 31.93498830348102 66-67 16.656192172061033 30.372542858140427 22.017387661045355 30.953877308753185 68-69 16.717293390593905 29.6812262141685 22.202437065744913 31.399043329492688 70-71 16.666666666666664 31.584092734192243 21.638560106141547 30.11068049299955 72-73 17.600642435669144 30.49649104430711 22.116895359798892 29.78597116022485 74-75 17.47669424950246 30.07925700918264 23.28654725742816 29.15750148388674 76 18.03707971090395 28.228762962187076 24.119269578576166 29.61488774833281 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.0 14 1.5 15 3.5 16 4.5 17 4.0 18 7.5 19 14.0 20 16.5 21 16.0 22 23.5 23 30.5 24 33.0 25 36.0 26 51.5 27 90.5 28 127.0 29 140.0 30 166.0 31 232.5 32 323.5 33 374.0 34 425.5 35 552.0 36 693.0 37 759.0 38 850.5 39 1057.5 40 1282.0 41 1502.0 42 1613.0 43 1686.5 44 1847.0 45 1942.0 46 1950.0 47 1956.5 48 1935.0 49 1834.0 50 1761.0 51 1678.0 52 1468.5 53 1312.0 54 1282.0 55 1140.5 56 928.5 57 764.5 58 671.0 59 599.0 60 477.0 61 391.0 62 355.0 63 306.5 64 227.5 65 171.0 66 121.5 67 98.0 68 92.0 69 75.0 70 50.0 71 36.0 72 28.5 73 23.5 74 16.5 75 7.0 76 11.5 77 12.0 78 6.5 79 5.0 80 7.5 81 6.5 82 2.5 83 2.0 84 2.0 85 3.0 86 2.0 87 0.0 88 2.5 89 4.0 90 2.5 91 2.5 92 3.0 93 1.5 94 1.0 95 1.5 96 1.0 97 1.0 98 1.0 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6703676547606577 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28641.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.47885897838763 #Duplication Level Percentage of deduplicated Percentage of total 1 89.10628216648966 76.16703327397786 2 7.2379707540233635 12.373869627457141 3 2.2751409198594885 5.83429349533885 4 0.8291806224981619 2.835096539925282 5 0.3022628870190344 1.2918543346950175 6 0.11845437464259455 0.6075206871268461 7 0.08986193938403726 0.5376907230892777 8 0.016338534433461317 0.11172794246010964 9 0.008169267216730659 0.06284696763381167 >10 0.016338534433461317 0.17806640829579973 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006982996403756852 0.0 2 0.0 0.0 0.0 0.006982996403756852 0.0 3 0.0 0.0 0.0 0.006982996403756852 0.0 4 0.0 0.0 0.0 0.010474494605635279 0.0 5 0.0 0.0 0.0 0.010474494605635279 0.0 6 0.0 0.0 0.0 0.010474494605635279 0.0 7 0.0 0.0 0.0 0.013965992807513703 0.0 8 0.0 0.0 0.0 0.013965992807513703 0.0 9 0.0 0.0 0.0 0.01745749100939213 0.0 10 0.0 0.0 0.0 0.01745749100939213 0.0 11 0.0 0.0 0.0 0.01745749100939213 0.0 12 0.0 0.0 0.0 0.01745749100939213 0.0 13 0.0 0.0 0.0 0.01745749100939213 0.0 14 0.0 0.0 0.0 0.01745749100939213 0.0 15 0.0 0.0 0.0 0.02444048741314898 0.0 16 0.0 0.0 0.0 0.027931985615027407 0.0 17 0.0 0.0 0.0 0.027931985615027407 0.0 18 0.0 0.0 0.0 0.04888097482629796 0.0 19 0.0 0.0 0.0 0.05935546943193324 0.0 20 0.0 0.0 0.0 0.05935546943193324 0.0 21 0.0 0.0 0.0 0.0663384658356901 0.0 22 0.0 0.0 0.0 0.06982996403756853 0.0 23 0.0 0.0 0.0 0.06982996403756853 0.0 24 0.0 0.0 0.0 0.07681296044132538 0.0 25 0.0 0.0 0.0 0.07681296044132538 0.0 26 0.0 0.0 0.0 0.07681296044132538 0.0 27 0.0 0.0 0.0 0.0942704514507175 0.0 28 0.0 0.0 0.0 0.11521944066198805 0.0 29 0.0 0.0 0.0 0.1326769316713802 0.0 30 0.0 0.0 0.0 0.15013442268077232 0.0 31 0.0 0.0 0.0 0.16410041548828602 0.0 32 0.0 0.0 0.0 0.19552389930519185 0.0 33 0.0 0.0 0.0 0.23742187772773296 0.0 34 0.0 0.0 0.0 0.28630285255403093 0.0 35 0.0 0.0 0.0 0.3247093327746936 0.0 36 0.0 0.0 0.0 0.3875563004085053 0.0 37 0.0 0.0 0.0 0.5272162284836424 0.0 38 0.0 0.0 0.0 0.7751126008170106 0.0 39 0.0 0.0 0.0 0.9880939911315946 0.0 40 0.0 0.0 0.0 1.295345832896896 0.0 41 0.0 0.0 0.0 1.6095806710659544 0.0 42 0.0 0.0 0.0 2.1612373869627457 0.0 43 0.0 0.0 0.0 2.688453615446388 0.0 44 0.0 0.0 0.0 3.1423483816905833 0.0 45 0.0 0.0 0.0 3.564819664117873 0.0 46 0.0 0.0 0.0 4.046646415977095 0.0 47 0.0 0.0 0.0 4.493558185817534 0.0 48 0.0 0.0 0.0 4.895080479033553 0.0 49 0.0 0.0 0.0 5.348975245277749 0.0 50 0.0 0.0 0.0 5.726057051080619 0.0 51 0.0 0.0 0.0 6.172968820921057 0.0 52 0.0 0.0 0.0 6.797946999057295 0.0 53 0.0 0.0 0.0 7.457840159212318 0.0 54 0.0 0.0 0.0 7.950141405677176 0.0 55 0.0 0.0 0.0 8.45291714674767 0.0 56 0.0 0.0 0.0 8.864913934569323 0.0 57 0.0 0.0 0.0 9.259453231381586 0.0 58 0.0 0.0 0.0 9.699382004818268 0.0 59 0.0 0.0 0.0 10.041548828602354 0.0 60 0.0 0.0 0.0 10.331343179358262 0.0 61 0.0 0.0 0.0 10.663035508536714 0.0 62 0.0 0.0 0.0 11.04710031074334 0.0 63 0.0 0.0 0.0 11.417199120142453 0.0 64 0.0 0.0 0.0 11.776823434935931 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGATC 15 0.002204037 69.98951 8 ATCAAAA 20 7.803175E-5 69.98951 12 CATTGGG 20 0.006887352 52.492134 39 TTGTAGA 20 0.006887352 52.492134 3 CCCGACG 20 0.006887352 52.492134 61 GGGCATT 30 5.7875185E-4 46.65967 36 ACCACCC 80 8.3927756E-5 26.246067 2 CGACGAA 75 0.0017914578 23.329838 70 ACCACAC 125 0.0017082635 16.797482 2 CCACACC 155 3.889927E-4 15.804083 3 TGCCGAC 150 0.005756269 13.997902 68 CTGCCGA 160 0.008824217 13.123034 67 >>END_MODULE