FastQCFastQC Report
Wed 25 May 2016
SRR1780484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28641
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT21567.527670123249887TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT16025.593380119409239No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC7392.580217171188157No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC3031.057923955169163No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT2080.7262316259907126No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1810.6319611745399951No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT1010.352641318389721No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT980.34216682378408575No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT790.27582835794839566No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC770.26884536154463884No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC750.26186236514088196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC720.2513878705352467No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG700.2444048741314898No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG600.20948989211270558No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT580.20250689570894873No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT580.20250689570894873No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC560.19552389930519187No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT490.17108341189204287No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG410.14315142627701546No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC390.1361684298732586No Hit
TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC380.1326769316713802No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCG150.002202727670.022
CTTTATA150.002202727670.02
GAATTTG150.002202727670.020
CTTATAA150.002202727670.01
CTTAATA150.002202727670.01
TCTTTAT150.002202727670.01
GTTGCGG150.002202727670.023
CTTTACA950.055.263161
TTTACAC950.055.263162
TTACACA1000.052.5000043
CTATACA407.2287E-752.51
GGTTTGA200.00688327152.51
CACCAGA200.00688327152.54
TTAATAC200.00688327152.52
TCCCCTT200.00688327152.513
TTATACA7400.027.4324322
CTTATAC7400.027.4324321
GGTGATC6900.026.37681233
GATCTCG6900.026.37681236
ATCTCGT7000.026.037
Produced by FastQC (version 0.11.5)