FastQCFastQC Report
Wed 25 May 2016
SRR1780483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296607
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC207.888612E-443.95282
TCAATGC250.00236234735.162243
ATGACCT353.181524E-431.47976516
CGACAAT353.1878392E-431.46912417
AGATGTC300.005725636629.3463940
ATTGTCT300.00576829229.3018678
CGGTCGT554.9273804E-628.01246510
TTGTACA508.381583E-526.371682
CTTATAA450.001394853724.447071
TTACACA1900.024.2897053
TGAAAGT551.5885042E-424.00257924
TATACCG551.6012821E-423.9742556
ACGTCCG651.8224884E-523.66689143
TTTACAC2050.022.512412
TATGTCA500.00256923622.00979240
CTTTACA2300.022.0023611
GCCACTA500.002586923321.9838128
GACGTCC703.2272736E-521.98010442
GGTATGA500.002589458121.98010341
CTTTATA602.886582E-421.97642
Produced by FastQC (version 0.11.5)