FastQCFastQC Report
Wed 25 May 2016
SRR1780483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296607
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3088010.411082678426336No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT241578.14444702923397TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC75862.557593043994242No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG39971.347574399795015No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC38811.3084654104589575No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT22000.7417222115459177No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC18310.6173151678820796No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC14570.49122239191927364No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9870.33276355581628214No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC9360.3155690863668086No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6950.23431678955655125No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6800.2292595926596473No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC5320.17936191661019465No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5240.17666474493184584No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3900.1314871193195036No Hit
CTTATCACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC3660.12339560428445721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCCA207.821584E-444.029021
CCGTCTT27450.042.71701444
CTTATAA950.041.71171
TTTACAC7450.040.1604582
GCCGTCT30050.039.02103443
CTATACA4350.037.4499661
CTTTACA8200.037.0488051
TGGGAGC553.4069672E-935.9994528
CTCGTGG505.9209924E-835.1994638
TCGTGGA505.9209924E-835.1994639
ACGCTGG250.00235011735.1994636
GAGCCAT505.9209924E-835.19946314
TATGTCG250.00235011735.19946326
GCGACAG451.0338372E-634.221735
TTACACA8750.034.193763
TCTTTAT1750.033.965241
CCTCGTG551.3679892E-731.9995127
CGCTGGA353.212344E-431.428097
TCGAGTG353.212344E-431.4280944
CTTATAC46150.031.1017511
Produced by FastQC (version 0.11.5)