FastQCFastQC Report
Wed 25 May 2016
SRR1780482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70984
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGG200.00692917952.492627
GGCGTCC200.00692917952.492619
GTCTCTG200.00692917952.492637
TTCGTCT200.00692917952.492642
CCTCGTG200.00692917952.492626
TCGTGGA200.00692917952.492628
GCCGAAC200.00692917952.492623
GAGCCAT200.00692917952.492633
GAGCCAG200.00692917952.492615
ATTGGAT305.8460387E-446.6600937
TTGGATC350.001248688839.9943638
TGGATCA350.001248688839.9943639
GTAGATC501.6957961E-434.99506838
GGTGGTC400.00240588134.99506847
TAGATCT400.00240588134.99506839
GATCTCG400.00240588134.99506841
AGATCTC450.004284485231.10672640
CCCTCGT450.004284485231.10672625
CATAATG450.004284485231.10672632
ACATAAT450.004284485231.10672631
Produced by FastQC (version 0.11.5)