FastQCFastQC Report
Wed 25 May 2016
SRR1780482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70984
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT34904.916600924151922No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT31684.462977572410684No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC10521.4820241181111238No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC5750.810041699537924No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG2670.3761411022202186No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2530.3564183477966866No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC2100.29584131635298094No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT1840.2592133438521357No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1500.21131522596641497No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1490.20990645779330555No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT1210.17046094894624142No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT1120.15778203538825653No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT1090.1535557308689282No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT1010.14228558548405273No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC980.13805928096472445No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT950.13383297644539613No Hit
TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC910.12819790375295842No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC840.11833652654119238No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG730.10284007663698862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA150.002216287270.047
CTGTAAC150.002216287270.027
CTTTTCG150.002216287270.048
TTTCGCT150.002216287270.050
CTAAGGA150.002216287270.036
AATTGCA150.002216287270.052
TTAAGCC150.002216287270.066
CTGTTTT150.002216287270.060
GTCCGGG150.002216287270.045
CGGGAAT150.002216287270.048
TTGCATC150.002216287270.054
GAATTGC150.002216287270.051
AAGGCTT150.002216287270.065
TTTTCGC150.002216287270.049
AGTGTCT150.002216287270.08
GGTAGCA150.002216287270.065
CAAAGGT150.002216287270.061
TCAGTAA150.002216287270.03
TCCGGGA150.002216287270.046
CTATACA1350.057.037041
Produced by FastQC (version 0.11.5)