FastQCFastQC Report
Wed 25 May 2016
SRR1780481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295104
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAA250.002357750735.1761432
TATACTG250.002359711835.1701775
TATACGC250.002359711835.1701773
TTAATAC401.8259941E-532.9720463
CTTAATA353.2216083E-431.412592
CGACATG300.00575711429.3134528
TTGACAT300.00575711429.3134527
TTACGTT300.00575711429.3134531
TCCGCTT454.0780695E-529.31344831
ACTATTG300.005761884629.3084833
CCGATAC300.005761884629.30848344
AACGGCC407.0264057E-427.49533737
GCCGAAA407.033374E-427.49067723
GTATAAC407.0543145E-427.4767043
TTATACG508.3492974E-526.3865762
TCGGTGG1600.024.72903342
CTGCGGA450.001379239724.49428718
CGGACGA450.001383342524.48180821
GATGTAG450.001391578224.4568861
TGTTCGA450.001394332524.4485910