FastQCFastQC Report
Wed 25 May 2016
SRR1780481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295104
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT213287.227282585122532No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT159845.416395575797007Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC47691.6160404467577532No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC27380.9278085014096725RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG23020.7800639774452396No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT14120.47847538494903485No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC13630.4618710691823899No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC8890.3012497289091303No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG7310.24770928215137714No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC5320.18027542832357407No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC4500.15248861418347429No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG4270.1446947516807634No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG4060.13757861635220126No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3690.12504066363044894RNA PCR Primer, Index 47 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG207.848151E-443.99864611
TTGCGCC207.848151E-443.99864613
CCGTCTT17350.042.47707744
CTTATAA452.3239409E-839.1430661
TTTACAC5700.038.987862
GCCGTCT19450.037.8908643
CTATACA4300.036.867311
ATCGTAC301.2989875E-436.6655425
TCGTACT301.2989875E-436.6655426
CATCGTA301.2989875E-436.6655424
AGTGGCG250.002350276335.1989176
TGACGAT250.002350276335.19891740
TGTGACG250.002350276335.19891738
CGAGGAT250.002350276335.19891724
CGATAAC250.002350276335.19891743
ATCGCCT250.002350276335.19891734
TCTATCG250.002350276335.19891731
CTTTACA6400.034.747121
GCGGAAT401.8158198E-532.99898519
CGTAGCG551.3681347E-731.99901215
Produced by FastQC (version 0.11.5)