FastQCFastQC Report
Wed 25 May 2016
SRR1780480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90765
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC150.002218843469.995593
CTCTGAA150.002218843469.9955921
ACGGTCA150.002218843469.9955911
GGAGCAA200.006933252352.49669334
ACGCCGA200.006933252352.49669360
AACTTCG200.006933252352.49669333
TACGGTC200.006933252352.49669310
TCAATGC200.006933252352.49669315
ACTACGG200.006933252352.4966938
GTCAATG200.006933252352.49669314
GGTATAC200.006933252352.4966933
TCCCGAC200.006933252352.49669319
CGGCTCA200.006933252352.49669338
TTCGGCT352.0729389E-549.99685336
TAGATCT305.852484E-446.66372336
GATCTCG305.852484E-446.66372338
AGATCTC350.001250054939.99748237
CCCTAAG654.6430068E-737.8567961
CTGGGGT400.002408494534.99779526
TCAGAGC652.0837708E-532.305663
Produced by FastQC (version 0.11.5)