##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780480_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 90765 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.85832644741916 34.0 24.0 34.0 12.0 34.0 2 29.83853908444885 34.0 31.0 34.0 12.0 34.0 3 30.01830000550873 34.0 31.0 34.0 11.0 34.0 4 30.389522392992895 34.0 32.0 34.0 21.0 34.0 5 30.403173029251363 34.0 32.0 34.0 11.0 34.0 6 32.83881452101581 37.0 34.0 38.0 11.0 38.0 7 31.963080482564866 37.0 28.0 38.0 11.0 38.0 8 31.471745716961383 37.0 27.0 38.0 11.0 38.0 9 31.997168512091665 37.0 31.0 38.0 12.0 38.0 10-11 32.385297196055745 37.0 31.0 38.0 11.0 38.0 12-13 32.70624139260728 37.0 32.5 38.0 11.0 38.0 14-15 32.27582217815237 37.0 31.0 38.0 11.0 38.0 16-17 32.12999504214179 37.0 31.0 38.0 11.0 38.0 18-19 32.161438880625795 37.0 31.0 38.0 11.0 38.0 20-21 32.27424117225803 37.0 31.0 38.0 11.0 38.0 22-23 32.14820139921776 37.0 31.0 38.0 11.0 38.0 24-25 32.56223764666997 37.0 32.0 38.0 11.0 38.0 26-27 32.586046383517875 37.0 32.0 38.0 11.0 38.0 28-29 32.65503222607833 37.0 33.5 38.0 11.0 38.0 30-31 32.531322646394536 37.0 32.0 38.0 11.0 38.0 32-33 32.34280835123671 37.0 31.5 38.0 11.0 38.0 34-35 32.24113920564094 37.0 31.0 38.0 11.0 38.0 36-37 32.198237205971466 37.0 31.0 38.0 11.0 38.0 38-39 32.61204208670743 37.0 33.0 38.0 11.0 38.0 40-41 32.19300941993059 37.0 31.0 38.0 11.0 38.0 42-43 32.53739877706164 37.0 31.5 38.0 11.0 38.0 44-45 31.61904368423952 37.0 28.5 38.0 11.0 38.0 46-47 32.30423621439982 37.0 31.0 38.0 11.0 38.0 48-49 32.122255274610254 37.0 31.0 38.0 11.0 38.0 50-51 32.07921004792596 37.0 29.0 38.0 11.0 38.0 52-53 32.09061312179805 37.0 31.0 38.0 11.0 38.0 54-55 31.85840356965791 37.0 30.5 38.0 11.0 38.0 56-57 31.89739987880791 37.0 29.0 38.0 11.0 38.0 58-59 31.77431829449678 37.0 29.0 38.0 11.0 38.0 60-61 31.808957197157493 37.0 29.0 38.0 11.0 38.0 62-63 31.828832699829228 37.0 30.5 38.0 11.0 38.0 64-65 31.890712278962155 37.0 31.0 38.0 11.0 38.0 66-67 31.92103233625296 37.0 30.5 38.0 11.0 38.0 68-69 31.482118658073045 37.0 28.0 38.0 11.0 38.0 70-71 31.4211645458051 37.0 27.0 38.0 11.0 38.0 72-73 31.420470445656363 37.0 27.0 38.0 11.0 38.0 74-75 30.991703850603205 36.0 26.5 38.0 11.0 38.0 76 30.085572632622707 35.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.7421863130063784 1101 2 -0.5442066658384839 1101 3 0.010546022126622034 1101 4 -1.4669519783326912 1101 5 -0.6432524732499658 1101 6 -1.642770152535789 1101 7 -1.2798946244459302 1101 8 -0.6482544931891852 1101 9 -0.7106373493733962 1101 10-11 -0.5619456758847292 1101 12-13 -1.1904394407004375 1101 14-15 -0.0013152094340895815 1101 16-17 -1.6788515418007002 1101 18-19 -0.21537895350056857 1101 20-21 -0.8018732847020829 1101 22-23 -0.33311559613493813 1101 24-25 -0.33200035318888155 1101 26-27 -0.8603713064535583 1101 28-29 0.0020854029098700266 1101 30-31 -1.435421013774949 1101 32-33 -0.05987920454695228 1101 34-35 -0.6754241009303605 1101 36-37 0.5782109753234366 1101 38-39 -0.6901906840982122 1101 40-41 0.3514874156788963 1101 42-43 -0.3025102551989107 1101 44-45 0.3185259694512048 1101 46-47 0.45096540808840757 1101 48-49 -0.30152029576291994 1101 50-51 -1.4738008543838887 1101 52-53 -0.09645002481457254 1101 54-55 -1.5019376258694415 1101 56-57 -0.6038430094757175 1101 58-59 -0.30678372054471126 1101 60-61 0.21979041838411817 1101 62-63 -0.8957112466935335 1101 64-65 -0.1016635794091485 1101 66-67 -1.5892063692719987 1101 68-69 -0.1177476283481731 1101 70-71 0.35776994250910477 1101 72-73 0.02502467423243715 1101 74-75 -1.9748517548411186 1101 76 -2.3538721432457166 1102 1 0.8051690215325031 1102 2 -0.41856621978664776 1102 3 -0.23785286274378237 1102 4 -0.09551210491497031 1102 5 0.10610708069819808 1102 6 -0.15173639726153 1102 7 1.110702120581191 1102 8 2.105079593610089 1102 9 1.4193543625012275 1102 10-11 -0.38997649264181433 1102 12-13 -4.0383240850645095 1102 14-15 -1.4095148025624837 1102 16-17 -2.5553584736873773 1102 18-19 -0.6137458160683771 1102 20-21 0.13709597388743333 1102 22-23 -0.7853176003543467 1102 24-25 0.17073096085572104 1102 26-27 -4.858553569865251 1102 28-29 -0.5881233071564367 1102 30-31 -0.019357119862945638 1102 32-33 -6.346149171394455 1102 34-35 -0.3379090316114919 1102 36-37 0.12509539364772593 1102 38-39 0.053009285763153 1102 40-41 0.3763141185119281 1102 42-43 -0.16530410691681396 1102 44-45 -5.311267957613293 1102 46-47 -0.9017696732859157 1102 48-49 0.08306755836607493 1102 50-51 -6.202816827861888 1102 52-53 -1.9260819567012533 1102 54-55 0.5104474705742028 1102 56-57 -1.6659662765040508 1102 58-59 0.2179487990454021 1102 60-61 -0.013962972212627989 1102 62-63 -4.306887030298114 1102 64-65 -0.9244389712114369 1102 66-67 -0.05764556457037173 1102 68-69 -7.406127299837021 1102 70-71 -0.8064667964902874 1102 72-73 -0.019825386044839632 1102 74-75 -6.768769401013511 1102 76 -2.3046331438484913 1103 1 0.6292963709763058 1103 2 -0.017962077093894635 1103 3 0.5913090091396107 1103 4 0.6173283246976133 1103 5 0.6373211198236284 1103 6 -0.9836792434448789 1103 7 0.754737410186106 1103 8 1.7566426929579997 1103 9 0.11579663331058399 1103 10-11 0.3167745622105045 1103 12-13 -0.7313620597480579 1103 14-15 0.1501323013884175 1103 16-17 -0.9164056543548114 1103 18-19 0.10760535385873737 1103 20-21 -0.12736029768909418 1103 22-23 -0.09211180825614917 1103 24-25 0.12295094551241448 1103 26-27 -0.3300006354578855 1103 28-29 0.6978375674120301 1103 30-31 0.3172846139306813 1103 32-33 -4.485677364720118 1103 34-35 -0.7233137113199675 1103 36-37 -0.12051737965492038 1103 38-39 0.48959827694074676 1103 40-41 0.761119450310936 1103 42-43 0.49213260194394337 1103 44-45 -3.9799724071721734 1103 46-47 0.0472857544087546 1103 48-49 0.20456736657474295 1103 50-51 0.5435151629321311 1103 52-53 0.18824965051010167 1103 54-55 0.18116572910891549 1103 56-57 0.8390682459355361 1103 58-59 0.8145636820526931 1103 60-61 1.0748364140551097 1103 62-63 0.5785176494103617 1103 64-65 0.6640967019761312 1103 66-67 0.29546624977562175 1103 68-69 -1.823848818824363 1103 70-71 0.49210435809352404 1103 72-73 1.1885798690376284 1103 74-75 -0.8665590059233672 1103 76 1.1166365147629413 1104 1 -2.3288621371822025 1104 2 0.40016146602964753 1104 3 0.6666586594892614 1104 4 0.5931441755134621 1104 5 0.27199477949728745 1104 6 -0.2716163063819437 1104 7 1.5832921887408808 1104 8 1.858682319997623 1104 9 0.7784423209562696 1104 10-11 0.04049251092845907 1104 12-13 -0.10764411103010474 1104 14-15 -0.5450308687747523 1104 16-17 -0.35824714619630527 1104 18-19 1.062471321224706 1104 20-21 1.1624124295836324 1104 22-23 0.6902017115573713 1104 24-25 0.9601012951627652 1104 26-27 1.3266135836563393 1104 28-29 0.43828045785492264 1104 30-31 -0.6925638709178017 1104 32-33 -0.19226244630520029 1104 34-35 0.40465831665206053 1104 36-37 0.8002576786201416 1104 38-39 0.33391645875892806 1104 40-41 1.2810203827118656 1104 42-43 0.7854018327131698 1104 44-45 -1.9996402393400032 1104 46-47 0.5395643091873055 1104 48-49 1.7655813525887325 1104 50-51 1.147215629132596 1104 52-53 0.25564475540520704 1104 54-55 0.47769836314155256 1104 56-57 1.3962943531616432 1104 58-59 1.3287245212135304 1104 60-61 0.9390555282742206 1104 62-63 0.9063148522075686 1104 64-65 0.2773542777337106 1104 66-67 0.6207867625961327 1104 68-69 -3.6962847162602586 1104 70-71 -0.03831522232605877 1104 72-73 0.8746812676390299 1104 74-75 -0.5139657658301324 1104 76 0.13225423038065642 1105 1 -1.0788621371822025 1105 2 -0.2575308416626605 1105 3 -0.9025721097415094 1105 4 -0.0030096706403845985 1105 5 0.6912255487280561 1105 6 -0.17546246022809697 1105 7 0.6832921887408823 1105 8 -0.025933064617756685 1105 9 0.6861346286485777 1105 10-11 0.6770309724669232 1105 12-13 -1.0691825725685646 1105 14-15 -0.527723176467056 1105 16-17 0.7917528538036933 1105 18-19 0.40093285968624315 1105 20-21 -0.054895262724059535 1105 22-23 0.7709709423265991 1105 24-25 -0.03797562791415743 1105 26-27 0.11315204519479494 1105 28-29 -0.9828733882989198 1105 30-31 -0.3021792555331899 1105 32-33 0.619276015233261 1105 34-35 0.16811985511360206 1105 36-37 0.3791038324662921 1105 38-39 0.06853184337432339 1105 40-41 -0.0709026942112132 1105 42-43 0.7257864480977858 1105 44-45 0.9638212991215305 1105 46-47 0.7337950784180762 1105 48-49 0.42135058335795605 1105 50-51 -0.03932283240586543 1105 52-53 -0.6943552445947958 1105 54-55 -0.6549939445507569 1105 56-57 -0.17870564683835255 1105 58-59 -0.5578139403249338 1105 60-61 -0.22825216403346715 1105 62-63 0.45631485220756574 1105 64-65 0.3869696623490917 1105 66-67 0.2515559933653577 1105 68-69 0.3017922068166641 1105 70-71 1.111684777673947 1105 72-73 -0.11570334774558333 1105 74-75 -0.3274273042916711 1105 76 -0.902361154234729 1106 1 -0.09887517499184995 1106 2 0.22349914529953097 1106 3 0.3630732618360675 1106 4 -0.08188841901065658 1106 5 0.09539764781541038 1106 6 0.17714510170149822 1106 7 1.3012191770068533 1106 8 1.1791516811449547 1106 9 1.5861998176968193 1106 10-11 0.724260437916719 1106 12-13 0.4600221210429041 1106 14-15 0.5793824297910888 1106 16-17 0.5172743661896178 1106 18-19 0.5858090004945851 1106 20-21 -0.265553672111281 1106 22-23 0.3329331326786189 1106 24-25 0.8458900826464699 1106 26-27 0.12143105432126333 1106 28-29 -0.7572214978165235 1106 30-31 -0.10612319295169925 1106 32-33 0.5906580230559513 1106 34-35 0.7741824365999079 1106 36-37 0.7981716290764638 1106 38-39 0.5072215435046914 1106 40-41 -0.051508952359846205 1106 42-43 0.6393783646558049 1106 44-45 0.516657022719972 1106 46-47 0.5682474904128654 1106 48-49 0.3615396315978572 1106 50-51 0.22247638532554959 1106 52-53 -0.04836437106154534 1106 54-55 0.3811207881741403 1106 56-57 0.08759161522161918 1106 58-59 -0.09317899899507154 1106 60-61 0.7120346677787914 1106 62-63 0.3394960777616767 1106 64-65 0.4612525828184566 1106 66-67 1.030988848645677 1106 68-69 1.2340607856954087 1106 70-71 0.4969194582476071 1106 72-73 -0.11351951462954801 1106 74-75 -1.1113907984246545 1106 76 -1.118853983439422 1107 1 0.20064417739873974 1107 2 0.19206732136833793 1107 3 0.3279100946212914 1107 4 0.1034771261449201 1107 5 -0.11273541568066747 1107 6 -0.08866567492384547 1107 7 0.44718426681206225 1107 8 0.4151117115016447 1107 9 -0.8218447054501574 1107 10-11 -0.13160852236660503 1107 12-13 0.6859264974658714 1107 14-15 0.5129771679646282 1107 16-17 1.0980674347451398 1107 18-19 1.3790040422350458 1107 20-21 0.39935272809109534 1107 22-23 -0.05443089464240103 1107 24-25 -0.41122476683494114 1107 26-27 0.4061198982372787 1107 28-29 -0.7947562815250961 1107 30-31 -0.04442954255959819 1107 32-33 0.62145741592213 1107 34-35 0.5816387988564173 1107 36-37 0.2567157727647924 1107 38-39 0.40555824980371113 1107 40-41 0.5869618293249559 1107 42-43 0.5453042437349893 1107 44-45 0.035376982244383015 1107 46-47 0.4297767087280633 1107 48-49 0.13977767865072366 1107 50-51 0.7041042628869008 1107 52-53 1.003463354716338 1107 54-55 1.091246009525019 1107 56-57 1.0410130672833446 1107 58-59 1.0526912261503867 1107 60-61 0.047982049514178726 1107 62-63 -0.3141214279302069 1107 64-65 -0.24242184166927316 1107 66-67 1.0544836627109433 1107 68-69 1.4964248130164357 1107 70-71 0.502959174918491 1107 72-73 0.7543023927825381 1107 74-75 0.4182385969712463 1107 76 -0.12239559740350003 1108 1 -0.7920734611741942 1108 2 -0.7601187741762452 1108 3 -1.1939647319891513 1108 4 -0.9315198221988616 1108 5 -0.6936043054326504 1108 6 -0.17669208562129057 1108 7 0.040870112675683856 1108 8 0.560869909359937 1108 9 0.02152210362713447 1108 10-11 0.08319339740257448 1108 12-13 -0.24468300150765998 1108 14-15 0.13081271143686024 1108 16-17 -0.6744038519440885 1108 18-19 -0.4707643093157543 1108 20-21 -0.1387028120520526 1108 22-23 -0.09769505710148252 1108 24-25 -0.579461186964771 1108 26-27 -0.4155940228635444 1108 28-29 0.042098015761702356 1108 30-31 -0.07044491181000012 1108 32-33 0.40192542901650796 1108 34-35 -0.2282985034794791 1108 36-37 -0.09450211548909948 1108 38-39 -0.15223595759794506 1108 40-41 0.22174100038415645 1108 42-43 -0.49601795567686935 1108 44-45 0.05494225994509705 1108 46-47 0.04964437781756459 1108 48-49 -0.6976800142971769 1108 50-51 -0.2932047311762389 1108 52-53 -0.48007157287618085 1108 54-55 -0.3773774160977972 1108 56-57 0.32150882270697423 1108 58-59 -0.532470789052411 1108 60-61 0.018623729589638316 1108 62-63 -0.3551592684673004 1108 64-65 0.18804201007571564 1108 66-67 0.24953997963931585 1108 68-69 1.0522354438769987 1108 70-71 0.7746744831357724 1108 72-73 0.543661822400253 1108 74-75 0.236720536714909 1108 76 -0.018403476225007154 1109 1 -0.23422299254305656 1109 2 -0.7954132295450478 1109 3 0.5652033580339584 1109 4 0.33794667031595793 1109 5 -0.10391849641598938 1109 6 0.5025001177344848 1109 7 -0.4497787943301006 1109 8 0.5545688658841712 1109 9 0.83533272784668 1109 10-11 0.8257835712195174 1109 12-13 0.3674152890292959 1109 14-15 0.5118966631527755 1109 16-17 -0.23338822133737835 1109 18-19 0.2778039615573462 1109 20-21 1.021485788656996 1109 22-23 0.8963124926681516 1109 24-25 0.9490083340698021 1109 26-27 0.4422951993379485 1109 28-29 0.04684891642337874 1109 30-31 0.6311813028273718 1109 32-33 0.9746888456460923 1109 34-35 0.16410292609666755 1109 36-37 0.2347022880647458 1109 38-39 0.4361365359790099 1109 40-41 0.2711302728217575 1109 42-43 0.7732619455732888 1109 44-45 0.20191158721182134 1109 46-47 -0.6675191478961509 1109 48-49 -0.32553091352354 1109 50-51 0.8777918097087749 1109 52-53 0.5908689906294384 1109 54-55 -0.008996023552835908 1109 56-57 0.3959156777829662 1109 58-59 0.0630280555170657 1109 60-61 0.5386248778435778 1109 62-63 -0.11970979881708743 1109 64-65 0.006022231401662026 1109 66-67 0.15397654578591613 1109 68-69 0.5307200357444977 1109 70-71 0.04418403517319902 1109 72-73 -0.1531847852270225 1109 74-75 1.7385290366646693 1109 76 -0.12534897722255067 1110 1 0.18294570478128236 1110 2 -0.008249189462720352 1110 3 -0.3482470573619807 1110 4 0.4986365430680877 1110 5 0.2872297390902254 1110 6 0.5629691470751084 1110 7 0.07474684303600299 1110 8 1.733016351724654 1110 9 -0.9522820130754539 1110 10-11 -0.5424991083472079 1110 12-13 0.1476911179432534 1110 14-15 -0.03245229948412032 1110 16-17 0.32469209355227946 1110 18-19 0.41777661903973495 1110 20-21 -0.16054476885994973 1110 22-23 0.16937710814521978 1110 24-25 0.4129447552046699 1110 26-27 -0.5979598973373754 1110 28-29 0.2898668092467247 1110 30-31 0.4632727049576886 1110 32-33 1.3455630550417013 1110 34-35 0.9944966883970494 1110 36-37 0.6117886573690186 1110 38-39 0.9017180151791635 1110 40-41 0.5001463928884604 1110 42-43 1.0444185941618471 1110 44-45 1.747853026149368 1110 46-47 0.5983042074213714 1110 48-49 1.2624909604905525 1110 50-51 0.795689140057469 1110 52-53 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-0.4011732273397186 2116 40-41 -0.7785173889335297 2116 42-43 -0.4268696402085226 2116 44-45 0.5540420649028412 2116 46-47 -0.29443017166816077 2116 48-49 -0.08701437007426449 2116 50-51 -0.2760510145376358 2116 52-53 0.273375007216174 2116 54-55 -0.5245092946714855 2116 56-57 -0.676032311205379 2116 58-59 -0.9949043163166493 2116 60-61 -2.2866257411290185 2116 62-63 -0.7591301288203773 2116 64-65 -0.1023542424456636 2116 66-67 -0.8116899535128681 2116 68-69 -0.38065001463900927 2116 70-71 -3.7845466814498927 2116 72-73 -0.3519572283940846 2116 74-75 -2.387698087320299 2116 76 -1.5924611887293807 2117 1 0.2845994012793369 2117 2 0.6743779902461711 2117 3 0.9667298845604861 2117 4 0.3744832068524957 2117 5 -1.3248000922975827 2117 6 -1.2168442266098616 2117 7 -3.3096565292078353 2117 8 -2.565747879432571 2117 9 0.21512323263718613 2117 10-11 0.14547826591420687 2117 12-13 0.4399342365482397 2117 14-15 -0.8448171935610773 2117 16-17 -0.4235605365096937 2117 18-19 0.23113228988567158 2117 20-21 -0.27508757041636756 2117 22-23 0.3260279223835809 2117 24-25 0.6549374774989474 2117 26-27 0.8697047517475056 2117 28-29 0.7496052441797119 2117 30-31 0.5315102031562731 2117 32-33 1.5534640494213008 2117 34-35 -0.011402936909192363 2117 36-37 -1.2070072786448236 2117 38-39 0.4329905328330099 2117 40-41 -1.8194781927867112 2117 42-43 -1.2023118425004995 2117 44-45 -0.15526701996678227 2117 46-47 -0.7332277136047125 2117 48-49 -0.21273061322323628 2117 50-51 0.6918737487907123 2117 52-53 0.7543270915875411 2117 54-55 -1.3828073348641468 2117 56-57 -0.42692502005773036 2117 58-59 -0.4924649374759298 2117 60-61 1.1922606564793519 2117 62-63 -0.4690412731485587 2117 64-65 -0.16060868522925276 2117 66-67 -0.887404120594752 2117 68-69 -0.48588585586139743 2117 70-71 0.7309511594403268 2117 72-73 1.110507478465241 2117 74-75 0.3421952028308404 2117 76 -0.8383298152033873 2118 1 0.45737717905711506 2118 2 0.7117853976535784 2118 3 0.3969150697456705 2118 4 0.7604091327784204 2118 5 0.4868665743690812 2118 6 1.747785403019769 2118 7 1.4331212485699432 2118 8 -3.7403775090622027 2118 9 -1.4895063969924465 2118 10-11 -0.05878099334504583 2118 12-13 1.105304606918608 2118 14-15 -0.6537060824499648 2118 16-17 0.6055135375643843 2118 18-19 -0.7397936360402504 2118 20-21 -1.9442542370830367 2118 22-23 -0.16137948502382216 2118 24-25 0.14651155157302043 2118 26-27 1.0139640110067631 2118 28-29 0.2546052441797073 2118 30-31 0.19076946241553117 2118 32-33 0.6629084938657357 2118 34-35 1.4173933593871055 2118 36-37 0.49021494357739925 2118 38-39 -0.14330576346328883 2118 40-41 -0.8663300446385627 2118 42-43 -0.5503673980560606 2118 44-45 0.5339922392924699 2118 46-47 0.7519574715804751 2118 48-49 -0.8896750576676844 2118 50-51 -0.1186818067648403 2118 52-53 -0.37854327878282845 2118 54-55 0.875988961432153 2118 56-57 1.669926831794129 2118 58-59 1.4485535810425887 2118 60-61 -0.8627393435206479 2118 62-63 0.706699467592184 2118 64-65 0.6212431666226017 2118 66-67 -1.8664781946688223 2118 68-69 1.2339289589534168 2118 70-71 -0.11451180352263535 2118 72-73 -0.6324554844977222 2118 74-75 0.87432483246047 2118 76 0.3403738885003129 2119 1 0.2961847671329956 2119 2 -0.004528965490052883 2119 3 0.15333783397331402 2119 4 0.3332004606916996 2119 5 0.7067039727430675 2119 6 0.47613228648859973 2119 7 -1.1683963666062098 2119 8 1.0582132768456596 2119 9 0.5023635217067444 2119 10-11 0.013522529690177976 2119 12-13 0.27392249309747285 2119 14-15 -0.9819445648347873 2119 16-17 -0.08939161148710895 2119 18-19 -0.8867313054169443 2119 20-21 -0.9205143996846594 2119 22-23 0.12458257459676503 2119 24-25 -0.2448705622481171 2119 26-27 0.3821482928495783 2119 28-29 0.6239277374046424 2119 30-31 0.05255808030170783 2119 32-33 0.09662123099311515 2119 34-35 0.5353337387908965 2119 36-37 0.08885993002726877 2119 38-39 -0.0020591509971623623 2119 40-41 0.152314941811305 2119 42-43 -0.21914788586093437 2119 44-45 -0.46497795040942336 2119 46-47 0.2936918889246485 2119 48-49 -0.39243928530996186 2119 50-51 0.6011555916091424 2119 52-53 -0.9698711920619623 2119 54-55 -0.38065060496351677 2119 56-57 0.49193225184832556 2119 58-59 0.9587432829396079 2119 60-61 0.594496428837072 2119 62-63 0.351577516372668 2119 64-65 0.6816225704166321 2119 66-67 0.5880475505886267 2119 68-69 -0.3173447537837113 2119 70-71 -0.6178180365849677 2119 72-73 -1.8519134790776697 2119 74-75 0.0069535587477282945 2119 76 1.0758752435138632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 26.0 13 129.0 14 544.0 15 1067.0 16 1669.0 17 1997.0 18 2247.0 19 2245.0 20 1834.0 21 1587.0 22 1300.0 23 1130.0 24 1003.0 25 1116.0 26 1289.0 27 1345.0 28 1613.0 29 1948.0 30 2353.0 31 3057.0 32 3803.0 33 4955.0 34 6952.0 35 10167.0 36 16640.0 37 18747.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.72334805673318 24.786362728342176 9.703016447295408 27.787272767629233 2 20.103564149176446 22.36985622211205 29.39899741089627 28.127582217815238 3 17.277584972180907 23.225913072219466 28.525312620503495 30.971189335096128 4 12.57863713986669 18.98749517986008 33.80267724343084 34.631190436842395 5 11.831653170274887 36.98231697240126 32.61499476670523 18.571035090618633 6 26.14333718944527 36.14609155511486 18.182118658073048 19.528452597366826 7 24.459868892194127 30.237426320718335 19.785159477772268 25.517545309315263 8 21.696689252465156 36.911805211259846 18.533575717512257 22.85792981876274 9 22.07568996860023 21.188784222993444 19.169283314052773 37.56624249435355 10-11 22.308158431113316 26.314107860959624 26.016636368644303 25.36109733928276 12-13 22.194127692392442 24.501184377237923 25.662424943535505 27.642262986834133 14-15 21.0692447529334 25.909216107530437 23.019335647000496 30.002203492535667 16-17 21.658128133090948 28.022916322370957 24.667548063680933 25.65140748085716 18-19 20.445105492205144 27.40869277805321 25.587506197322757 26.558695532418884 20-21 20.031399768633285 27.636203382361042 25.43821957803118 26.894177270974495 22-23 22.04759543877045 27.477001046658955 25.360546466148847 25.114857048421747 24-25 20.991571641051067 28.786426485980275 25.00853853357572 25.213463339392938 26-27 19.844102903101415 29.250812537872527 26.274995868451494 24.63008869057456 28-29 21.089076185754422 27.645568225637636 27.487467636203384 23.777887952404562 30-31 21.142510879744396 27.40373491984796 27.370131658679007 24.083622541728637 32-33 19.488789731724783 29.24310031399769 26.598358398060927 24.669751556216603 34-35 20.345397454966122 28.351236710185646 26.69861730843387 24.604748526414365 36-37 20.82410620834022 27.404285792981874 26.88756679336749 24.884041205310417 38-39 20.61091830551424 26.878201950090897 28.00804274775519 24.502836996639672 40-41 20.0479259626508 26.47110670412604 27.923208285131935 25.55775904809122 42-43 19.146697515562167 25.746708533024844 28.827741971024075 26.278851980388918 44-45 18.853633008318184 25.658017958464164 29.57803117941938 25.91031785379827 46-47 18.958298903762465 25.87781633889715 29.390734313887513 25.773150443452874 48-49 18.190381755081805 26.54602545033879 29.066820911144163 26.196771883435243 50-51 17.860959620999285 26.90905084559026 28.457555225031676 26.772434308378777 52-53 17.412548889990635 26.831377733707928 28.716465597972785 27.03960777832865 54-55 17.215887181182175 27.171266457334873 27.90558034484658 27.707266016636368 56-57 17.527130501845427 27.49407811381039 27.470941442185865 27.50784994215832 58-59 17.65107695697681 26.66225968159533 27.6444664793698 28.04219688205806 60-61 17.529884867515012 25.853027047870874 27.98876218806809 28.628325896546023 62-63 17.757946344956757 26.541067592133533 27.26877100203823 28.432215060871478 64-65 18.807359665069136 26.595053159257425 25.48779816008373 29.10978901558971 66-67 18.51870214289649 27.604803613727757 24.92756018288988 28.948934060485872 68-69 18.378780366881507 27.248388696083293 24.766154354652123 29.60667658238308 70-71 18.719770836776288 28.011347986558693 24.40973943700766 28.859141739657357 72-73 19.426541067592133 28.238307717732607 23.69966396738831 28.63548724728695 74-75 19.362088910923813 28.913678179915163 23.750895168842614 27.973337740318406 76 19.876604418002533 27.63840687489671 24.51385445931802 27.971134247782736 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 2.0 14 2.5 15 3.5 16 9.0 17 14.0 18 15.0 19 16.5 20 23.0 21 29.0 22 36.0 23 52.0 24 78.5 25 96.0 26 140.0 27 213.0 28 335.0 29 428.0 30 534.0 31 746.5 32 1022.5 33 1192.0 34 1336.5 35 1745.5 36 2235.5 37 2461.0 38 2823.5 39 3585.5 40 4464.5 41 5227.0 42 5510.0 43 5559.5 44 6034.5 45 6662.0 46 6864.0 47 6812.0 48 6621.5 49 6145.0 50 5807.0 51 5530.5 52 4782.5 53 4038.0 54 3765.0 55 3388.5 56 2693.0 57 2074.5 58 1775.0 59 1542.5 60 1124.0 61 843.5 62 749.0 63 647.0 64 459.5 65 323.5 66 235.0 67 197.0 68 165.0 69 111.5 70 82.5 71 75.0 72 58.0 73 36.0 74 29.0 75 27.0 76 18.5 77 12.5 78 12.5 79 10.0 80 9.0 81 8.0 82 5.5 83 3.0 84 4.0 85 3.5 86 3.0 87 4.0 88 4.0 89 5.5 90 4.5 91 3.0 92 4.0 93 2.5 94 2.0 95 2.5 96 2.0 97 2.0 98 4.0 99 7.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7260507905029472 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 90765.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5531868010797 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99238498849472 53.23748140803173 2 9.772046286026454 13.007216438054318 3 4.236264009138014 8.458106098165592 4 2.1851772145612265 5.817220294166253 5 1.150528912212161 3.8285682807249493 6 0.7548794013938782 3.0143777887952408 7 0.5578823646266161 2.5990194458216274 8 0.36088532785935407 1.9214454911033991 9 0.21520684688860564 1.2890431333663859 >10 0.7747446487989803 6.827521621770506 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.002203492535669035 0.0 3 0.0 0.0 0.0 0.002203492535669035 0.0 4 0.0 0.0 0.0 0.003305238803503553 0.0 5 0.0 0.0 0.0 0.00440698507133807 0.0 6 0.0 0.0 0.0 0.005508731339172589 0.0 7 0.0 0.0 0.0 0.005508731339172589 0.0 8 0.0 0.0 0.0 0.005508731339172589 0.0 9 0.0 0.0 0.0 0.007712223874841624 0.0 10 0.0 0.0 0.0 0.012119208946179695 0.0 11 0.0 0.0 0.0 0.01432270148184873 0.0 12 0.0 0.0 0.0 0.01432270148184873 0.0 13 0.0 0.0 0.0 0.015424447749683249 0.0 14 0.0 0.0 0.0 0.015424447749683249 0.0 15 0.0 0.0 0.0 0.015424447749683249 0.0 16 0.0 0.0 0.0 0.016526194017517766 0.0 17 0.0 0.0 0.0 0.01762794028535228 0.0 18 0.0 0.0 0.0 0.02313667162452487 0.0 19 0.0 0.0 0.0 0.02313667162452487 0.0 20 0.0 0.0 0.0 0.02864540296369746 0.0 21 0.0 0.0 0.0 0.029747149231531978 0.0 22 0.0 0.0 0.0 0.03415413430287005 0.0 23 0.0 0.0 0.0 0.0374593731063736 0.0 24 0.0 0.0 0.0 0.03966286564204264 0.0 25 0.0 0.0 0.0 0.04517159698121523 0.0 26 0.0 0.0 0.0 0.056189059659560406 0.0 27 0.0 0.0 0.0 0.07932573128408528 0.0 28 0.0 0.0 0.0 0.11127637305128629 0.0 29 0.0 0.0 0.0 0.14212526855065277 0.0 30 0.0 0.0 0.0 0.17627940285352284 0.0 31 0.0 0.0 0.0 0.23797719385225583 0.0 32 0.0 0.0 0.0 0.29526799977965074 0.0 33 0.0 0.0 0.0 0.35696579077838375 0.0 34 0.0 0.0 0.0 0.43188453699113094 0.0 35 0.0 0.0 0.0 0.5222277309535613 0.0 36 0.0 0.0 0.0 0.7238472979672781 0.0 37 0.0 0.0 0.0 1.0367432380322812 0.0 38 0.0 0.0 0.0 1.5160028645402963 0.0 39 0.0 0.0 0.0 2.029416625351182 0.0 40 0.0 0.0 0.0 2.6915661323197266 0.0 41 0.0 0.0 0.0 3.5211810719991186 0.0 42 0.0 0.0 0.0 4.72869498154575 0.0 43 0.0 0.0 0.0 5.775353935988542 0.0 44 0.0 0.0 0.0 6.804384950145981 0.0 45 0.0 0.0 0.0 7.7684129345011845 0.0 46 0.0 0.0 0.0 8.678455351732495 0.0 47 0.0 0.0 0.0 9.584090783892469 0.0 48 0.0 0.0 0.0 10.353109678840964 0.0 49 0.0 0.0 0.0 11.08907618575442 0.0 50 0.0 0.0 0.0 11.796397289704181 0.0 51 0.0 0.0 0.0 12.590756348812869 0.0 52 0.0 0.0 0.0 13.497493527240676 0.0 53 0.0 0.0 0.0 14.540847242879964 0.0 54 0.0 0.0 0.0 15.565471271966066 0.0 55 0.0 0.0 0.0 16.36864430121743 0.0 56 0.0 0.0 0.0 17.13435795736242 0.0 57 0.0 0.0 0.0 17.85600176279403 0.0 58 0.0 0.0 0.0 18.578747314493473 0.0 59 0.0 0.0 0.0 19.16597807524927 0.0 60 0.0 0.0 0.0 19.745496612130225 0.0 61 0.0 0.0 0.0 20.398832148956096 0.0 62 0.0 0.0 0.0 21.09072880515617 0.0 63 0.0 0.0 0.0 21.849831983694155 0.0 64 0.0 0.0 0.0 22.56596705778659 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGTC 15 0.0022188434 69.99559 3 CTCTGAA 15 0.0022188434 69.99559 21 ACGGTCA 15 0.0022188434 69.99559 11 GGAGCAA 20 0.0069332523 52.496693 34 ACGCCGA 20 0.0069332523 52.496693 60 AACTTCG 20 0.0069332523 52.496693 33 TACGGTC 20 0.0069332523 52.496693 10 TCAATGC 20 0.0069332523 52.496693 15 ACTACGG 20 0.0069332523 52.496693 8 GTCAATG 20 0.0069332523 52.496693 14 GGTATAC 20 0.0069332523 52.496693 3 TCCCGAC 20 0.0069332523 52.496693 19 CGGCTCA 20 0.0069332523 52.496693 38 TTCGGCT 35 2.0729389E-5 49.996853 36 TAGATCT 30 5.852484E-4 46.663723 36 GATCTCG 30 5.852484E-4 46.663723 38 AGATCTC 35 0.0012500549 39.997482 37 CCCTAAG 65 4.6430068E-7 37.856796 1 CTGGGGT 40 0.0024084945 34.997795 26 TCAGAGC 65 2.0837708E-5 32.30566 3 >>END_MODULE