FastQCFastQC Report
Wed 25 May 2016
SRR1780480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90765
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT33273.6655098330854403No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT30733.385666281055473No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC11611.279127416955875No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC5110.5629923428634385No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG3360.370186745992398No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT2030.2236544923704071No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1700.18729686553186803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1560.17187241778218476No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1300.1432270148184873No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT1090.12009034319396245No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT1080.11898859692612793No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT1070.11788685065829341No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT1000.11017462678345176No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC990.10907288051561724No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC970.1068693879799482No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG920.10136065664077562No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATC150.00221828970.033
TTTACAC1800.056.3888932
CTATACA750.056.01
CTGGGAA252.3792803E-455.99999631
TAACTCG700.055.036
CTGTCCA200.00693152652.510
GAATCTC200.00693152652.535
GCTTGAC200.00693152652.553
TACATAT200.00693152652.54
AATCTCG200.00693152652.536
TTCTGTT200.00693152652.538
AGGTGAG200.00693152652.536
GACTAGC200.00693152652.510
CTTTACA1900.051.5789451
ACTCGTA750.051.33333238
AACTCGT750.051.33333237
GTAAGGT352.0721667E-550.019
CTTTATA451.6685608E-646.6666682
CTATCGG305.850667E-446.66666438
GCTATCG305.850667E-446.66666437
Produced by FastQC (version 0.11.5)