##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 90765 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.713072219467854 34.0 34.0 34.0 31.0 34.0 2 33.28115463008869 34.0 34.0 34.0 32.0 34.0 3 33.40650030298023 34.0 34.0 34.0 33.0 34.0 4 33.47410345397455 34.0 34.0 34.0 34.0 34.0 5 33.5098441029031 34.0 34.0 34.0 34.0 34.0 6 37.25281771607999 38.0 37.0 38.0 36.0 38.0 7 36.90405993499697 38.0 37.0 38.0 35.0 38.0 8 36.89553241888393 38.0 37.0 38.0 35.0 38.0 9 36.676736627554675 38.0 37.0 38.0 34.0 38.0 10-11 36.39023852806699 38.0 37.0 38.0 34.0 38.0 12-13 36.26178042196882 38.0 37.0 38.0 34.0 38.0 14-15 36.47080923263373 38.0 37.0 38.0 34.0 38.0 16-17 36.593020437393264 38.0 37.0 38.0 34.0 38.0 18-19 36.633261719825924 38.0 37.0 38.0 34.0 38.0 20-21 36.37592133531648 38.0 37.0 38.0 34.0 38.0 22-23 36.423786701922545 38.0 37.0 38.0 34.0 38.0 24-25 35.673805982482236 38.0 36.5 38.0 31.5 38.0 26-27 35.91809067371784 38.0 37.0 38.0 32.0 38.0 28-29 35.796882058062025 38.0 36.5 38.0 31.5 38.0 30-31 35.49921776014984 38.0 36.0 38.0 30.5 38.0 32-33 35.97868671844874 38.0 37.0 38.0 33.0 38.0 34-35 35.84772764832259 38.0 36.5 38.0 32.0 38.0 36-37 35.91140307387209 38.0 37.0 38.0 32.5 38.0 38-39 35.44853743182945 38.0 36.5 38.0 29.5 38.0 40-41 35.234043959676086 37.0 36.0 38.0 29.0 38.0 42-43 35.743783396683746 38.0 36.5 38.0 32.5 38.0 44-45 35.96303090398281 38.0 37.0 38.0 33.0 38.0 46-47 36.22186966341651 38.0 37.0 38.0 34.0 38.0 48-49 36.001349639178095 38.0 37.0 38.0 33.0 38.0 50-51 36.093764116124056 38.0 37.0 38.0 34.0 38.0 52-53 36.001382691566135 38.0 37.0 38.0 33.0 38.0 54-55 36.215964303420925 38.0 37.0 38.0 34.0 38.0 56-57 36.23703520079326 38.0 37.0 38.0 34.0 38.0 58-59 36.25109899190217 38.0 37.0 38.0 34.0 38.0 60-61 35.97880240180686 38.0 37.0 38.0 32.5 38.0 62-63 36.303993830220904 38.0 37.0 38.0 34.0 38.0 64-65 36.294309480526636 38.0 37.0 38.0 34.0 38.0 66-67 35.48194237867019 38.0 36.0 38.0 31.0 38.0 68-69 34.37755742852421 37.5 34.5 38.0 24.0 38.0 70-71 33.7949374758993 37.0 34.0 38.0 22.0 38.0 72-73 33.10555830992122 37.0 34.0 38.0 16.0 38.0 74-75 33.54942433757506 37.5 34.0 38.0 21.0 38.0 76 33.07942488844819 37.0 34.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5724458849297918 1101 2 0.4638196659414362 1101 3 0.21149124665873842 1101 4 0.33572971418800535 1101 5 0.3197561503811599 1101 6 -1.2487940212496156 1101 7 0.0538938060961911 1101 8 0.6255733519796891 1101 9 0.24564364637058134 1101 10-11 -0.09090529077047194 1101 12-13 0.5020473682541677 1101 14-15 0.7976652255212358 1101 16-17 -0.1654732591902004 1101 18-19 -0.3131817095345326 1101 20-21 -0.11757754598255588 1101 22-23 0.6612347894097681 1101 24-25 -1.6608746286603449 1101 26-27 -0.20639658927149185 1101 28-29 0.4742309970724108 1101 30-31 1.2209523487043512 1101 32-33 0.2666204310500646 1101 34-35 0.46153034983213814 1101 36-37 0.6052241621165493 1101 38-39 0.619050415355332 1101 40-41 1.2478713909406096 1101 42-43 1.1235735748427516 1101 44-45 0.5288181956150382 1101 46-47 0.7983859386944161 1101 48-49 -1.785262185631126 1101 50-51 -1.4391381526046914 1101 52-53 -1.234690384368946 1101 54-55 -0.9817344786782556 1101 56-57 -1.430251544147744 1101 58-59 -1.2987723607472503 1101 60-61 -1.6206813580653758 1101 62-63 -0.8604461124955236 1101 64-65 -0.9135175069933155 1101 66-67 -1.3072093743917392 1101 68-69 -0.8301179277408792 1101 70-71 -0.6724994130730906 1101 72-73 -1.6240756112832386 1101 74-75 -1.1684416878211366 1101 76 -1.9063743411798164 1102 1 -1.0130913362878076 1102 2 0.008688562867298799 1102 3 0.2707701797509614 1102 4 0.42593842425431205 1102 5 0.10518788636669285 1102 6 0.21607562914218192 1102 7 -0.20477105225221237 1102 8 0.3410081018289972 1102 9 0.603257419728024 1102 10-11 0.5300354114597852 1102 12-13 0.32784604215410695 1102 14-15 -0.08860850865598024 1102 16-17 0.03513704822389485 1102 18-19 -0.3916269325604418 1102 20-21 0.4966667276762706 1102 22-23 0.5968982161728817 1102 24-25 -4.830451557533166 1102 26-27 -1.2558522101274292 1102 28-29 0.3702244419187011 1102 30-31 1.5088705223029066 1102 32-33 0.45357416824114694 1102 34-35 0.34180461143551355 1102 36-37 -2.926054092856326 1102 38-39 -0.5376234092377956 1102 40-41 0.7397998870225919 1102 42-43 0.6826264681519731 1102 44-45 0.8313347718657909 1102 46-47 1.0972952967414287 1102 48-49 -3.0860175352393213 1102 50-51 -0.6353218476016735 1102 52-53 -0.05877492324778899 1102 54-55 0.39194695592090056 1102 56-57 0.8685447940077751 1102 58-59 0.05682589121175852 1102 60-61 -5.908203962043679 1102 62-63 -2.4150818117119286 1102 64-65 -0.09515930928385075 1102 66-67 0.9904944381459373 1102 68-69 -0.6859233828343037 1102 70-71 -1.16510602850407 1102 72-73 -5.396349948233208 1102 74-75 -2.769547399092989 1102 76 -0.882169247750042 1103 1 0.7178733741072847 1103 2 0.48519412481589796 1103 3 -0.04405529013779841 1103 4 0.26118967089796286 1103 5 -0.056731944856935 1103 6 0.1471745934689963 1103 7 0.6301925073948951 1103 8 0.5391285467848803 1103 9 0.19004299701992977 1103 10-11 0.4137619170217377 1103 12-13 -0.18382925512245407 1103 14-15 -0.3779976532666396 1103 16-17 0.03230812609118061 1103 18-19 0.5254952385174221 1103 20-21 -0.3393037364587457 1103 22-23 0.2584344647344494 1103 24-25 -2.720195516106237 1103 26-27 -0.42575535550525956 1103 28-29 -0.19155634058992632 1103 30-31 0.7419209634229702 1103 32-33 1.0968422925219272 1103 34-35 0.4028182286200135 1103 36-37 -0.9430036517362623 1103 38-39 0.03578472271463795 1103 40-41 -0.2643715744273507 1103 42-43 -0.5480985030793235 1103 44-45 1.025571442368289 1103 46-47 0.6282695967030705 1103 48-49 -2.0431761466700777 1103 50-51 -0.23716304437958513 1103 52-53 0.306299875371316 1103 54-55 0.04478067283690024 1103 56-57 0.562781464510266 1103 58-59 -0.19108837806327017 1103 60-61 -3.983506033390057 1103 62-63 -0.9733384285128466 1103 64-65 0.3060441813183772 1103 66-67 0.05081281175544916 1103 68-69 0.39350220212925535 1103 70-71 0.7258366258879505 1103 72-73 -4.018353208685838 1103 74-75 -2.074759328513778 1103 76 -2.8115420900975643 1104 1 -0.23311730188339652 1104 2 0.1664295560513267 1104 3 0.422961026878518 1104 4 0.08799619770449141 1104 5 -0.10352681665180796 1104 6 0.22613729743169841 1104 7 0.5651575423599269 1104 8 0.5838837915401243 1104 9 0.9498332068101405 1104 10-11 -0.41490941164959594 1104 12-13 0.33583857704537223 1104 14-15 0.8075209947520037 1104 16-17 0.14757619135924216 1104 18-19 0.7855476860698687 1104 20-21 0.0574911353361216 1104 22-23 0.3537141850141694 1104 24-25 0.9418382834275647 1104 26-27 1.307374015124111 1104 28-29 1.583880722347132 1104 30-31 0.8853067443087568 1104 32-33 0.5499308706105026 1104 34-35 0.3346072729090608 1104 36-37 1.4080744368418223 1104 38-39 1.4723814593113786 1104 40-41 1.618029357973576 1104 42-43 0.6713070913262627 1104 44-45 0.9593127011095461 1104 46-47 0.9746565430900134 1104 48-49 0.09135182535788999 1104 50-51 0.5708206386041041 1104 52-53 0.1512299453013881 1104 54-55 0.263457829014051 1104 56-57 0.4285740053028064 1104 58-59 -0.6236349981098854 1104 60-61 0.24332963094560967 1104 62-63 0.38196460179018743 1104 64-65 0.6442434820176786 1104 66-67 0.30285243879507817 1104 68-69 1.7178903140173674 1104 70-71 1.6853645979159282 1104 72-73 1.1871537843211541 1104 74-75 0.6575541912997451 1104 76 0.5387492851938092 1105 1 0.8668826981166049 1105 2 0.3548910945128654 1105 3 0.21526871918620571 1105 4 -0.008157648449355293 1105 5 -0.5035268166518065 1105 6 0.20690652820093192 1105 7 0.6497729269753165 1105 8 -0.7276546699983371 1105 9 0.4498332068101405 1105 10-11 0.3004752037350258 1105 12-13 -0.3641614229546235 1105 14-15 0.02867484090585748 1105 16-17 0.15911465289770632 1105 18-19 0.25862460914679275 1105 20-21 0.23249113533611876 1105 22-23 -0.20205504575506694 1105 24-25 0.39183828342756044 1105 26-27 -0.20801060026050777 1105 28-29 0.5838807223471321 1105 30-31 1.1737682827702898 1105 32-33 1.0653154859951144 1105 34-35 -1.3307773424755496 1105 36-37 0.45422828299567897 1105 38-39 -0.23531084838093363 1105 40-41 0.362260127204344 1105 42-43 0.2520763220954976 1105 44-45 0.7362357780326221 1105 46-47 1.0169642353977153 1105 48-49 -0.47787894387288077 1105 50-51 0.6727437155271758 1105 52-53 0.5877684068398494 1105 54-55 0.9653809059371312 1105 56-57 0.6824201591489683 1105 58-59 0.5513650018901117 1105 60-61 0.7490988617148346 1105 62-63 -0.35457385974827815 1105 64-65 0.49039732817151815 1105 66-67 0.5586216695643103 1105 68-69 0.4525056986327485 1105 70-71 0.5892107517620744 1105 72-73 0.3813845535519249 1105 74-75 -0.44436888562333365 1105 76 0.06951851596303982 1106 1 -1.0228174322614905 1106 2 0.3023487216315104 1106 3 0.3710705444795579 1106 4 -0.11682779186526204 1106 5 0.09934150147074661 1106 6 0.17137849691018658 1106 7 -1.5493144263493264 1106 8 0.29952916311770394 1106 9 -0.3446257240894681 1106 10-11 0.40451692472588974 1106 12-13 1.128863348883705 1106 14-15 -0.18882841854655652 1106 16-17 0.33437540909066144 1106 18-19 0.6242047916761209 1106 20-21 0.5300465460271226 1106 22-23 0.6478797651966985 1106 24-25 -0.04900265529472847 1106 26-27 0.958971146805986 1106 28-29 0.7588481278230148 1106 30-31 1.087099443135351 1106 32-33 0.5041355642219756 1106 34-35 1.05475068881519 1106 36-37 1.2644629635693363 1106 38-39 0.3773358269776139 1106 40-41 1.1232118873086492 1106 42-43 1.1735887080146625 1106 44-45 1.165016742830538 1106 46-47 0.9902367256193543 1106 48-49 1.3014887223591955 1106 50-51 0.4430501040539099 1106 52-53 0.8413538044930391 1106 54-55 0.2862739958980143 1106 56-57 0.3349951265544391 1106 58-59 0.8545266707297472 1106 60-61 1.1556503610629463 1106 62-63 0.8620493475528974 1106 64-65 -0.6684357878649863 1106 66-67 0.5368811219763003 1106 68-69 1.6663257768596083 1106 70-71 0.4370269186460476 1106 72-73 -0.14961283888614219 1106 74-75 -0.46539235368070564 1106 76 -0.2499730094606889 1107 1 0.733759850814657 1107 2 -0.16824323384534523 1107 3 0.2603318649956208 1107 4 0.05234751802596094 1107 5 0.0371735277798777 1107 6 0.3168376418633869 1107 7 0.6149279212347878 1107 8 -0.3251862429030439 1107 9 -1.0769176542690744 1107 10-11 0.3259057433446628 1107 12-13 0.02656762411771041 1107 14-15 -0.03745604313548512 1107 16-17 0.42607791351768043 1107 18-19 0.5269368938769858 1107 20-21 0.6805967610536925 1107 22-23 -0.16235929374587954 1107 24-25 0.09835378285350771 1107 26-27 -0.319836088205399 1107 28-29 0.5841390461129237 1107 30-31 0.5524422207725834 1107 32-33 0.9427265078091267 1107 34-35 -0.3183203964365191 1107 36-37 0.871248948896941 1107 38-39 0.43684759019541985 1107 40-41 0.24150811801950312 1107 42-43 0.7577020396615168 1107 44-45 0.5450761913506526 1107 46-47 1.003043454685887 1107 48-49 0.6994459700191982 1107 50-51 1.0480020392929674 1107 52-53 0.8112184642451297 1107 54-55 0.8512879093814476 1107 56-57 0.8870125816518382 1107 58-59 0.8516807309371828 1107 60-61 1.01844444150818 1107 62-63 0.8666660943274991 1107 64-65 0.7895075463115901 1107 66-67 0.32122212880656065 1107 68-69 0.7709614965661586 1107 70-71 -0.24236215294515517 1107 72-73 0.5266773204864847 1107 74-75 -0.7329739372880866 1107 76 0.8336976204406525 1108 1 -0.6024910508110466 1108 2 0.38147102016342416 1108 3 -0.08041329396792207 1108 4 0.44441598609454047 1108 5 0.08344773296214214 1108 6 -0.4249922421736727 1108 7 0.025680848050519955 1108 8 -0.8142002805216393 1108 9 -0.3617481486373819 1108 10-11 0.5390153810298486 1108 12-13 0.9754939959758886 1108 14-15 0.3402418983836881 1108 16-17 -0.26727225016205836 1108 18-19 0.2995954412886306 1108 20-21 0.877393909142242 1108 22-23 -0.016531740064472444 1108 24-25 0.9093175513715153 1108 26-27 -0.24226853563368422 1108 28-29 -1.0085342047332233 1108 30-31 0.1490756891185896 1108 32-33 0.8352111108163882 1108 34-35 0.48615860262024313 1108 36-37 1.2680544196842618 1108 38-39 0.9632236097260147 1108 40-41 0.9292890091031154 1108 42-43 -0.3387372323797635 1108 44-45 0.7199360925879503 1108 46-47 0.7385827655664272 1108 48-49 0.8063718425154534 1108 50-51 0.8168529520155943 1108 52-53 -0.15829965149587366 1108 54-55 -0.3551080846262096 1108 56-57 0.27697263269772066 1108 58-59 0.3886612558220506 1108 60-61 0.8679006918549632 1108 62-63 0.7507378359909325 1108 64-65 0.4081268105850242 1108 66-67 0.39267943336184175 1108 68-69 0.8245388699224279 1108 70-71 -0.9394852734023473 1108 72-73 -0.3066051519098991 1108 74-75 -0.9348964807048361 1108 76 -0.3183996996503424 1109 1 -1.3123272810933706 1109 2 0.06396445358622316 1109 3 0.3720848760023614 1109 4 0.19444110414939786 1109 5 -0.1675749306999208 1109 6 -0.5365594652650643 1109 7 -0.29407916687677726 1109 8 0.396446904103243 1109 9 0.5971156540925904 1109 10-11 0.28174191000172755 1109 12-13 0.2510821172889166 1109 14-15 0.557498719729729 1109 16-17 0.6735711423541986 1109 18-19 0.5825108580330394 1109 20-21 0.6750141528591342 1109 22-23 0.45714453844451697 1109 24-25 -0.20026893367965215 1109 26-27 0.47826798601808207 1109 28-29 -1.4569345434681367 1109 30-31 0.490860649862654 1109 32-33 0.45351714919678443 1109 34-35 0.17147243477422336 1109 36-37 1.015395494162881 1109 38-39 0.6825759945059104 1109 40-41 0.6232936882379079 1109 42-43 0.8599394049585811 1109 44-45 0.34107688287372895 1109 46-47 0.4905312089646827 1109 48-49 0.6690693780754415 1109 50-51 -0.16392097113751447 1109 52-53 -0.5490002299287866 1109 54-55 -0.9452962547400361 1109 56-57 -0.3334248066960015 1109 58-59 -0.3067356812105757 1109 60-61 0.651519414135393 1109 62-63 -0.06513964531406202 1109 64-65 0.7135336513078485 1109 66-67 0.3405343514769896 1109 68-69 -0.2761845300574777 1109 70-71 0.10796632051764732 1109 72-73 -0.23634636417899912 1109 74-75 -0.7354663017207557 1109 76 -0.3507517543072325 1110 1 -0.4181217915571409 1110 2 -0.22117894439763575 1110 3 -0.2561470246030737 1110 4 0.06351251018578097 1110 5 0.2709269397085592 1110 6 -0.09532334309508883 1110 7 0.5471539506209311 1110 8 0.8343177933359982 1110 9 0.9768311116290604 1110 10-11 -0.3495472446337331 1110 12-13 0.8149990080540519 1110 14-15 0.9971423655990534 1110 16-17 0.45768544008716816 1110 18-19 0.11527680908692872 1110 20-21 0.7240879626333339 1110 22-23 -0.4639302328248007 1110 24-25 -0.3671784780211027 1110 26-27 1.25984333568681 1110 28-29 0.5986143350379436 1110 30-31 0.22194248211180678 1110 32-33 0.7662209035347729 1110 34-35 0.7207715949683191 1110 36-37 1.2909089175362354 1110 38-39 0.6020357544325989 1110 40-41 -0.9010742038342627 1110 42-43 0.2727363041368065 1110 44-45 0.9734402078440567 1110 46-47 0.8125344239640029 1110 48-49 1.4955796014728264 1110 50-51 -0.13693901419446064 1110 52-53 0.759872567270854 1110 54-55 -0.4746819494953769 1110 56-57 0.3115581417888933 1110 58-59 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-0.9472259024610494 2116 42-43 -0.7001572067075372 2116 44-45 -0.3144713623606137 2116 46-47 -0.6615455955784952 2116 48-49 0.09255051456106145 2116 50-51 -1.2364836042382592 2116 52-53 -0.14386319026259997 2116 54-55 -0.866543895718614 2116 56-57 -0.4603704583053272 2116 58-59 -2.1332935010419334 2116 60-61 -1.6818600896476923 2116 62-63 0.48465518474982616 2116 64-65 -1.499219781176528 2116 66-67 -0.6877046498561796 2116 68-69 -1.4647071333782904 2116 70-71 0.6687381750114767 2116 72-73 -0.2765319693870225 2116 74-75 -0.45148340787238794 2116 76 -1.97285816565131 2117 1 0.6711562023901081 2117 2 0.278965168586943 2117 3 0.16469891861640917 2117 4 -0.027530867822576965 2117 5 0.017342129217134072 2117 6 0.3062655025599028 2117 7 0.7278356050379955 2117 8 0.4957783784347143 2117 9 -0.43706138008444384 2117 10-11 -0.3869535711937502 2117 12-13 -0.6242753830685857 2117 14-15 -0.14504310781209284 2117 16-17 -0.8420605607775045 2117 18-19 -1.2760620005398238 2117 20-21 -0.08004447719949326 2117 22-23 -0.08521031641033261 2117 24-25 -2.097299893210611 2117 26-27 -1.4773125945625054 2117 28-29 -0.9560765426101341 2117 30-31 -0.5040807200787114 2117 32-33 -1.9627115795319412 2117 34-35 0.4893081276099167 2117 36-37 -0.9390053352379439 2117 38-39 0.5319968439267626 2117 40-41 0.32169744914167353 2117 42-43 0.1303526753718458 2117 44-45 -0.18612889433205027 2117 46-47 -0.893826362892888 2117 48-49 -0.5791609951549361 2117 50-51 -0.18434317905971653 2117 52-53 0.6103823669538073 2117 54-55 -0.4169196638634389 2117 56-57 0.3233033500321554 2117 58-59 -0.6843543713292561 2117 60-61 -0.4060649559489775 2117 62-63 -0.14905391672833446 2117 64-65 -0.8852436974694982 2117 66-67 -0.7831518347091944 2117 68-69 0.2967008553279058 2117 70-71 0.053277703329030146 2117 72-73 1.408414468081837 2117 74-75 1.0794915132370662 2117 76 1.1371111085556365 2118 1 -0.24662157538766394 2118 2 0.3493355389573125 2118 3 -0.5778936739761846 2118 4 0.10987653958483179 2118 5 0.20049027736528302 2118 6 0.14682105811546364 2118 7 0.6274652346676248 2118 8 0.5655931932495264 2118 9 -1.3852095282325934 2118 10-11 -0.7653794971196746 2118 12-13 -0.6877939015871064 2118 14-15 0.48329022552123746 2118 16-17 -2.531782782999727 2118 18-19 -0.7279138523916728 2118 20-21 -0.5276370697920854 2118 22-23 0.6563637576637404 2118 24-25 0.543163069752346 2118 26-27 -2.1874977797476873 2118 28-29 -0.14718765372124665 2118 30-31 -1.2131547941527927 2118 32-33 0.007751383431013892 2118 34-35 0.5643081276099124 2118 36-37 -2.808079409312022 2118 38-39 0.24533017726009376 2118 40-41 -0.10987662493240435 2118 42-43 0.6222045272237082 2118 44-45 -2.0398325980357512 2118 46-47 -0.04577080733733396 2118 48-49 0.33361678262284045 2118 50-51 -0.44221354943008606 2118 52-53 -1.3224880034165594 2118 54-55 -0.631456700900479 2118 56-57 0.8462663129951196 2118 58-59 -0.24500251947740992 2118 60-61 -0.18004643743045534 2118 62-63 -0.7180353982098211 2118 64-65 0.44475630253050014 2118 66-67 0.1210148319574671 2118 68-69 -0.7522806261535706 2118 70-71 0.7826295551808826 2118 72-73 1.371470023637393 2118 74-75 1.2476396613852145 2118 76 0.7328518492963738 2119 1 0.7993404842329284 2119 2 -0.9438893932920109 2119 3 -0.09767687180816154 2119 4 -0.3885516446970101 2119 5 -0.24677259526344386 2119 6 -0.8451572616677367 2119 7 -0.7949737897226257 2119 8 -0.06996236230602904 2119 9 -0.49144259056321715 2119 10-11 -0.3012602559272679 2119 12-13 -0.058579809446179354 2119 14-15 -0.36841709155193314 2119 16-17 -0.35096977486964676 2119 18-19 -0.18888946214777036 2119 20-21 -1.1297508909302962 2119 22-23 -0.10547906076444491 2119 24-25 0.9988270263919148 2119 26-27 0.1385184804149162 2119 28-29 0.0647906660619526 2119 30-31 1.0200159375545255 2119 32-33 0.37474325334972036 2119 34-35 -0.04029891846054312 2119 36-37 -0.40027453126324275 2119 38-39 -0.8753202292439681 2119 40-41 -0.40396876585381136 2119 42-43 0.2935866410448398 2119 44-45 0.36531645345475994 2119 46-47 0.2923863742344821 2119 48-49 -0.181396767512652 2119 50-51 0.8371902446078536 2119 52-53 0.35230874455091765 2119 54-55 0.4673508871754066 2119 56-57 -1.1234897845658551 2119 58-59 0.11895412008898631 2119 60-61 0.522175784791763 2119 62-63 -0.4107183250390847 2119 64-65 -0.1750810958434883 2119 66-67 0.23591998100895495 2119 68-69 0.639372490377589 2119 70-71 -1.8887300340253432E-4 2119 72-73 0.532228831225467 2119 74-75 0.0035204201927996337 2119 76 0.5048843696215783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 7.0 23 12.0 24 36.0 25 89.0 26 205.0 27 421.0 28 752.0 29 1147.0 30 1781.0 31 2721.0 32 4098.0 33 6170.0 34 9192.0 35 12605.0 36 19091.0 37 32435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.83468297251143 21.799151655373766 10.587781633889715 30.778383738225084 2 19.086652343965184 19.172588552856276 39.65515341816779 22.08560568501074 3 16.789511375530218 25.137442846912357 33.906241392607285 24.166804384950147 4 11.85699333443508 21.3342147303476 35.88718118217374 30.92161075304357 5 11.660882498760536 38.196441359554896 36.26287665950532 13.879799482179253 6 24.054426265631026 38.07965625516444 17.75684459868892 20.109072880515615 7 24.426816504159092 34.184983198369416 19.751005343469398 21.63719495400209 8 21.679061312179805 35.63598303310748 21.528122073486475 21.156833581226245 9 20.546466148845923 19.472263537707267 19.172588552856276 40.80868176059054 10-11 21.224590976698067 25.296094309480527 29.400099157164107 24.079215556657303 12-13 23.564700049578583 20.647826805486698 27.16190161405828 28.625571530876435 14-15 23.0512862887677 23.747589930039112 21.397014267614168 31.80410951357902 16-17 23.886960832920177 28.007491874621277 22.399052498209663 25.706494794248886 18-19 22.219467856552637 26.832479479975763 23.402743348206908 27.545309315264692 20-21 21.192640334930864 25.513689197377843 23.11298407976643 30.180686387924858 22-23 23.015479535063076 29.176995537927613 23.460585027268223 24.34693989974109 24-25 23.423676527295765 30.279292678896052 23.485925191428414 22.811105602379772 26-27 23.92662369856222 28.733542665124222 26.144438935713104 21.19539470060045 28-29 20.76185754420757 24.29019996694761 30.344295708698287 24.603646780146534 30-31 26.342753263923317 23.21159037073762 26.457885748912023 23.98777061642704 32-33 26.444113920564092 23.971795295543437 29.03707376191263 20.547017021979837 34-35 26.992232688811768 25.844213077728202 27.93587836721203 19.227675866248003 36-37 20.539304798104997 27.271525367707817 30.127251693934888 22.061918140252303 38-39 20.756899686002313 24.633393929378062 28.58315429956481 26.02655208505481 40-41 20.21924750729907 24.984300115683357 30.05233294772214 24.744119429295434 42-43 23.644576653996584 23.795515892689913 31.237261058778167 21.32264639453534 44-45 19.703079380818597 25.057566242494357 30.077122238748416 25.162232137938634 46-47 23.388971519858977 21.822288326998294 28.650911695036633 26.1378284581061 48-49 21.05271855891588 22.11810719991186 29.899741089627057 26.9294331515452 50-51 17.656034815182064 22.07954608053765 32.98573238583155 27.278686718448743 52-53 18.476284911584862 22.847463229218313 35.52140142125268 23.15485043794414 54-55 17.159698121522613 23.03586184101801 32.97030793808186 26.834132099377513 56-57 20.862667327714426 22.906957527681374 30.057841679061315 26.172533465542884 58-59 17.316696964689033 27.182834793147137 32.26739381920344 23.233074422960392 60-61 18.780917754641106 32.282267393819204 26.379110890761858 22.557703960777832 62-63 15.997906682091115 36.725059218861894 24.234561780421966 23.04247231862502 64-65 16.236985622211204 37.443948658623924 23.03255660221451 23.286509116950366 66-67 16.06676582383077 36.77188343524486 22.366000110174628 24.795350630749738 68-69 16.317413099763126 35.43381259295984 22.29328485649755 25.955489450779485 70-71 16.79832534567289 33.67212031069245 22.97857103509062 26.55098330854404 72-73 17.382801740759106 31.422905304908276 23.334435079601167 27.859857874731446 74-75 17.990965680603757 31.225692722965903 23.11298407976643 27.670357516663913 76 18.68561670247342 30.87423566352669 23.103619236489838 27.33652839751005 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 1.0 14 1.0 15 0.5 16 1.0 17 2.0 18 5.0 19 9.5 20 19.5 21 28.0 22 39.0 23 60.0 24 77.5 25 85.0 26 114.0 27 191.5 28 311.5 29 383.0 30 495.5 31 708.5 32 985.5 33 1162.0 34 1274.5 35 1629.0 36 2026.0 37 2181.0 38 2584.0 39 3344.5 40 4295.0 41 5881.0 42 6874.0 43 7738.0 44 8837.0 45 8542.5 46 8013.0 47 7530.5 48 6555.5 49 5606.5 50 5150.0 51 4742.0 52 4012.5 53 3332.5 54 2974.0 55 2678.0 56 2121.5 57 1575.0 58 1289.0 59 1091.5 60 712.5 61 494.0 62 457.0 63 383.0 64 254.0 65 181.0 66 127.5 67 92.0 68 80.5 69 57.5 70 30.5 71 15.0 72 11.5 73 6.0 74 3.5 75 3.0 76 1.5 77 0.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 90765.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.91522062469013 #Duplication Level Percentage of deduplicated Percentage of total 1 62.79780242349558 26.949815457500137 2 17.097966728280962 14.675260287555774 3 7.773670158143356 10.008263097008758 4 3.915074964058328 6.720652233790558 5 2.3233723557198607 4.985401861951193 6 1.5172520024645717 3.9067922657412 7 1.0474430067775724 3.146587340935383 8 0.7008626001232285 2.406213848950587 9 0.5493941261039228 2.121963311849281 >10 2.2181146025878005 13.791659780752493 >50 0.028239884986650237 0.9408913127306782 >100 0.020538098172109262 1.4532033272737288 >500 0.0025672622715136578 0.5629923428634385 >1k 0.007701786814540974 8.330303531096789 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 3327 3.6655098330854403 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 3073 3.385666281055473 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 1161 1.279127416955875 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 511 0.5629923428634385 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 336 0.370186745992398 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 203 0.2236544923704071 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 170 0.18729686553186803 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 156 0.17187241778218476 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 130 0.1432270148184873 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 109 0.12009034319396245 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 108 0.11898859692612793 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT 107 0.11788685065829341 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 100 0.11017462678345176 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 99 0.10907288051561724 No Hit TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 97 0.1068693879799482 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 92 0.10136065664077562 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.003305238803503553 0.0 3 0.0 0.0 0.0 0.003305238803503553 0.0 4 0.0 0.0 0.0 0.00440698507133807 0.0 5 0.0 0.0 0.0 0.006610477607007106 0.0 6 0.0 0.0 0.0 0.007712223874841624 0.0 7 0.0 0.0 0.0 0.007712223874841624 0.0 8 0.0 0.0 0.0 0.007712223874841624 0.0 9 0.0 0.0 0.0 0.00991571641051066 0.0 10 0.0 0.0 0.0 0.012119208946179695 0.0 11 0.0 0.0 0.0 0.01432270148184873 0.0 12 0.0 0.0 0.0 0.01432270148184873 0.0 13 0.0 0.0 0.0 0.015424447749683249 0.0 14 0.0 0.0 0.0 0.015424447749683249 0.0 15 0.0 0.0 0.0 0.016526194017517766 0.0 16 0.0 0.0 0.0 0.01762794028535228 0.0 17 0.0 0.0 0.0 0.01762794028535228 0.0 18 0.0 0.0 0.0 0.02313667162452487 0.0 19 0.0 0.0 0.0 0.02423841789235939 0.0 20 0.0 0.0 0.0 0.030848895499366497 0.0 21 0.0 0.0 0.0 0.03195064176720101 0.0 22 0.0 0.0 0.0 0.0374593731063736 0.0 23 0.0 0.0 0.0 0.03966286564204264 0.0 24 0.0 0.0 0.0 0.04296810444554619 0.0 25 0.0 0.0 0.0 0.04847683578471878 0.0 26 0.0 0.0 0.0 0.060596044730898475 0.0 27 0.0 0.0 0.0 0.08373271635542334 0.0 28 0.0 0.0 0.0 0.11678510439045887 0.0 29 0.0 0.0 0.0 0.15204098496116344 0.0 30 0.0 0.0 0.0 0.1928055968710406 0.0 31 0.0 0.0 0.0 0.2522998953341046 0.0 32 0.0 0.0 0.0 0.31399768633283753 0.0 33 0.0 0.0 0.0 0.3790007161350741 0.0 34 0.0 0.0 0.0 0.4539194623478213 0.0 35 0.0 0.0 0.0 0.5442626563102517 0.0 36 0.0 0.0 0.0 0.7546961934666446 0.0 37 0.0 0.0 0.0 1.0841183275491655 0.0 38 0.0 0.0 0.0 1.5854128794138709 0.0 39 0.0 0.0 0.0 2.104335371563929 0.0 40 0.0 0.0 0.0 2.802842505371013 0.0 41 0.0 0.0 0.0 3.682036027102958 0.0 42 0.0 0.0 0.0 4.943535503773481 0.0 43 0.0 0.0 0.0 6.068418443232524 0.0 44 0.0 0.0 0.0 7.15033327824602 0.0 45 0.0 0.0 0.0 8.161736352118107 0.0 46 0.0 0.0 0.0 9.120255605134137 0.0 47 0.0 0.0 0.0 10.085385335757175 0.0 48 0.0 0.0 0.0 10.903982812758223 0.0 49 0.0 0.0 0.0 11.681815677849391 0.0 50 0.0 0.0 0.0 12.43981711011954 0.0 51 0.0 0.0 0.0 13.298077452762628 0.0 52 0.0 0.0 0.0 14.2643089296535 0.0 53 0.0 0.0 0.0 15.377072660166364 0.0 54 0.0 0.0 0.0 16.470004957858205 0.0 55 0.0 0.0 0.0 17.33267228557263 0.0 56 0.0 0.0 0.0 18.129234837216988 0.0 57 0.0 0.0 0.0 18.89494849336198 0.0 58 0.0 0.0 0.0 19.639728970418112 0.0 59 0.0 0.0 0.0 20.272131328155126 0.0 60 0.0 0.0 0.0 20.8681760590536 0.0 61 0.0 0.0 0.0 21.578802401806865 0.0 62 0.0 0.0 0.0 22.30705668484548 0.0 63 0.0 0.0 0.0 23.10912796782901 0.0 64 0.0 0.0 0.0 23.856111937420813 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAATC 15 0.002218289 70.0 33 TTTACAC 180 0.0 56.388893 2 CTATACA 75 0.0 56.0 1 CTGGGAA 25 2.3792803E-4 55.999996 31 TAACTCG 70 0.0 55.0 36 CTGTCCA 20 0.006931526 52.5 10 GAATCTC 20 0.006931526 52.5 35 GCTTGAC 20 0.006931526 52.5 53 TACATAT 20 0.006931526 52.5 4 AATCTCG 20 0.006931526 52.5 36 TTCTGTT 20 0.006931526 52.5 38 AGGTGAG 20 0.006931526 52.5 36 GACTAGC 20 0.006931526 52.5 10 CTTTACA 190 0.0 51.578945 1 ACTCGTA 75 0.0 51.333332 38 AACTCGT 75 0.0 51.333332 37 GTAAGGT 35 2.0721667E-5 50.0 19 CTTTATA 45 1.6685608E-6 46.666668 2 CTATCGG 30 5.850667E-4 46.666664 38 GCTATCG 30 5.850667E-4 46.666664 37 >>END_MODULE