FastQCFastQC Report
Wed 25 May 2016
SRR1780479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682256
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC401.835836E-532.9553683
GACCGTG353.242032E-431.3860667
CATCGGA300.0057793229.295819
TCGTTGT901.4988473E-824.46157834
CATCGTT901.5237674E-824.42391432
GTGGTAC551.5795515E-424.02917514
CGTCATA551.5913986E-424.00272223
AGTGCGT651.7877914E-523.73331819
GTAGCGA852.3486245E-723.2950522
GTAAAGG703.2464413E-521.9702453
ATCGACC500.002599644221.9702454
ATGCCCG856.1320698E-620.6960829
TACGAAT654.905885E-420.32192838
CACGAGG909.904616E-619.57932914
GATGCGT901.0120344E-519.5305399
CACATCG1252.3192115E-819.3338177
TCGTCTT808.8978224E-519.27340314
GGCATTA809.003812E-519.243711
GTCATGC809.013963E-519.24088726
TTGTTCG809.0699694E-519.2253749
Produced by FastQC (version 0.11.5)