FastQCFastQC Report
Wed 25 May 2016
SRR1780479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682256
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT326484.785300532351493No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT248563.643207241856429Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC82261.2057057761309538No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC43190.6330468328603925No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG40610.5952311155929739No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT20750.30413803616237894No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC20440.29959428718838677No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC13350.19567435097675945No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG13200.19347576276353745No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9260.13572617902957246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT25200.041.37998644
GCCGTCT27900.037.37547343
CTTATAA1050.035.649571
TTTACAC11150.034.728192
CTTTACA11450.033.8455731
TTACACA11450.033.6261333
CTATACA6700.031.8780381
GCTTATA353.2002854E-431.4555031
CTTTATA2850.029.33482
GCTCCGT300.0057418829.3347972
TCTAATG300.00574393729.3326540
GCGAAAT554.954967E-628.00139811
GGCGAAA554.954967E-628.00139810
TTATACA54150.026.6532252
TCTTTAT3150.026.5624241
CGGCGAT757.0045644E-826.39938518
CGTGCGA609.741074E-625.66794810
CTTATAC56400.025.649621
TATCGGT801.3083627E-724.74942424
TATACAC58850.024.6741313
Produced by FastQC (version 0.11.5)