FastQCFastQC Report
Wed 25 May 2016
SRR1780478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50954
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTTG200.00675656952.806521
TTAATGC200.006916456452.49558628
GATCCAA200.006916456452.49558614
TATAGTG350.001245024439.9966355
TTATCCA459.083178E-538.885622
TAATACT400.002398860634.9970554
GCCACCC400.002398860634.99705531
TATCCAC552.955225E-431.8155083
AATAACA450.004272055331.10849625
AGATCTC604.920962E-429.16421140
ATTAAAA604.920962E-429.16421159
ATCTCGG500.007149821627.99764442
TCTCGGT500.007149821627.99764443
GATCTCG500.007149821627.99764441
TATAACA500.007149821627.9976443
TGTAGAT808.517045E-526.24779337
CGGTGGT700.001211128724.99789646
GTAGATC851.2859277E-424.70380438
CTTATCC750.001748811123.4695631
GTGTAGA800.002633945821.87316136
Produced by FastQC (version 0.11.5)