##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50954 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.884955057502847 33.0 23.0 34.0 12.0 34.0 2 28.556462691839698 34.0 23.0 34.0 11.0 34.0 3 28.86574164933077 34.0 23.0 34.0 11.0 34.0 4 29.220885504572752 34.0 27.0 34.0 11.0 34.0 5 29.197511480943596 34.0 27.0 34.0 11.0 34.0 6 31.464477764257957 37.0 27.0 38.0 11.0 38.0 7 30.66936844997449 37.0 26.0 38.0 11.0 38.0 8 29.858892334262276 36.0 23.0 38.0 11.0 38.0 9 30.614632806060367 36.0 26.0 38.0 12.0 38.0 10-11 30.776101974329787 37.0 26.0 38.0 11.0 38.0 12-13 31.05471601836951 37.0 26.0 38.0 11.0 38.0 14-15 30.67971111198336 37.0 26.0 38.0 11.0 38.0 16-17 30.439209875574047 37.0 25.0 38.0 11.0 38.0 18-19 30.532146642069318 36.5 25.0 38.0 11.0 38.0 20-21 30.64291321584174 37.0 25.5 38.0 11.0 38.0 22-23 30.46073909800997 37.0 25.0 38.0 11.0 38.0 24-25 30.773638968481375 37.0 26.0 38.0 11.0 38.0 26-27 30.733445853122426 37.0 26.0 38.0 11.0 38.0 28-29 30.744534285826433 37.0 26.0 38.0 11.0 38.0 30-31 30.601336499587866 37.0 25.5 38.0 11.0 38.0 32-33 30.505769910115006 37.0 25.5 38.0 11.0 38.0 34-35 30.459600816422657 37.0 25.0 38.0 11.0 38.0 36-37 30.405336185579152 37.0 25.0 38.0 11.0 38.0 38-39 30.691682694194764 37.0 26.0 38.0 11.0 38.0 40-41 30.376398320053383 37.0 24.0 38.0 11.0 38.0 42-43 30.67344075048083 37.0 25.5 38.0 11.0 38.0 44-45 29.925413117714015 36.0 23.5 38.0 11.0 38.0 46-47 30.532038701573967 37.0 25.5 38.0 11.0 38.0 48-49 30.38018605016289 36.0 25.0 38.0 11.0 38.0 50-51 30.33645052400204 36.5 24.5 38.0 11.0 38.0 52-53 30.330543235074774 36.0 24.5 38.0 11.0 38.0 54-55 30.149585901008756 36.0 24.0 38.0 11.0 38.0 56-57 30.108882521489974 36.0 24.0 38.0 11.0 38.0 58-59 29.986036425010795 36.0 23.5 38.0 11.0 38.0 60-61 30.059583153432506 36.0 24.0 38.0 11.0 38.0 62-63 30.02566039957609 36.0 24.0 38.0 11.0 38.0 64-65 30.13013698630137 36.0 24.0 38.0 11.0 38.0 66-67 30.15985006083919 36.0 24.0 38.0 11.0 38.0 68-69 29.76568081014248 36.0 23.5 38.0 11.0 38.0 70-71 29.814273658594026 36.0 24.0 38.0 11.0 38.0 72-73 29.71616556109432 36.0 23.0 38.0 11.0 38.0 74-75 29.29992738548495 34.5 23.0 38.0 11.0 38.0 76 28.472426109824546 34.0 21.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.6468382708867644 1101 2 -0.841896313281584 1101 3 -0.6018643751095851 1101 4 -2.0531478485343975 1101 5 -0.4918739535219814 1101 6 -0.9724594042317847 1101 7 -0.4898627840113896 1101 8 -0.14924938486198158 1101 9 -0.8189012635695292 1101 10-11 -1.4780456871175964 1101 12-13 -0.745376495412863 1101 14-15 -0.297628326590754 1101 16-17 -2.383472091074495 1101 18-19 -0.8417742422764185 1101 20-21 -1.2039848514857638 1101 22-23 0.19404227584813327 1101 24-25 -0.8039008193241521 1101 26-27 -0.6918589366874102 1101 28-29 -0.07873161791236782 1101 30-31 -0.6965049743526208 1101 32-33 -0.2880889203973034 1101 34-35 -0.40077971821616387 1101 36-37 0.10648812135322316 1101 38-39 -0.9884315373339874 1101 40-41 0.10789549819505595 1101 42-43 -0.6647008957480693 1101 44-45 0.09785292285889469 1101 46-47 0.20592716603714933 1101 48-49 -0.7611794150780291 1101 50-51 -1.1211794046280517 1101 52-53 -0.12073183492227813 1101 54-55 -2.143271517553103 1101 56-57 -0.7888899714484836 1101 58-59 -0.4425769223997804 1101 60-61 -1.062135631066628 1101 62-63 -2.0390038902794636 1101 64-65 -0.24037992550221077 1101 66-67 -1.041629946574922 1101 68-69 0.06666690399028852 1101 70-71 0.0499434567845789 1101 72-73 -1.23193745390952 1101 74-75 -1.9834824982720711 1101 76 -3.115441646117713 1102 1 1.0098708972978159 1102 2 -0.12440997448377189 1102 3 1.0058466145686147 1102 4 0.9619705485512107 1102 5 0.5529347896474199 1102 6 1.153102222969185 1102 7 0.6954438340821127 1102 8 1.1868161889085087 1102 9 0.8036852573776478 1102 10-11 -0.22537416313459957 1102 12-13 -3.2496873636581576 1102 14-15 -0.3132931717637959 1102 16-17 -1.9413348718880954 1102 18-19 -0.9894973752454561 1102 20-21 -0.15480452361691022 1102 22-23 -0.08130684376327935 1102 24-25 0.15062255651069734 1102 26-27 -4.109193484349827 1102 28-29 0.7383297057063345 1102 30-31 0.8655168835708764 1102 32-33 -5.376643868788317 1102 34-35 -0.7547870041906606 1102 36-37 -0.39162966856784465 1102 38-39 0.5962375579908468 1102 40-41 0.01861195235413149 1102 42-43 -0.24554485446088137 1102 44-45 -4.80308818218057 1102 46-47 0.018100815096044442 1102 48-49 0.07749696622130031 1102 50-51 -4.088453235836308 1102 52-53 -1.338036024357617 1102 54-55 0.7807418400668098 1102 56-57 -1.010383596220791 1102 58-59 0.35092641700520133 1102 60-61 0.1374697119813355 1102 62-63 -3.6847840967154077 1102 64-65 -0.3532214555568558 1102 66-67 0.2607076369101975 1102 68-69 -6.033484887145111 1102 70-71 0.03679834445913954 1102 72-73 0.06411597657013957 1102 74-75 -5.219092698636366 1102 76 -2.509673582972603 1103 1 0.08772747728205488 1103 2 0.43161839431055427 1103 3 0.5796842820233046 1103 4 1.2702447802296994 1103 5 0.7983248605694051 1103 6 -0.5747149559118228 1103 7 1.1175084518914105 1103 8 0.7258232811070897 1103 9 0.003212444138878112 1103 10-11 1.066745616219226 1103 12-13 -0.21840288769282878 1103 14-15 -0.3045461268465388 1103 16-17 0.39207726365170004 1103 18-19 0.41877685643303053 1103 20-21 0.7281741997873468 1103 22-23 0.3769279868119746 1103 24-25 0.4837982854311065 1103 26-27 -0.019989386635092643 1103 28-29 0.9803312817504626 1103 30-31 0.7789920608758436 1103 32-33 -2.945832206061759 1103 34-35 -0.6160163186114644 1103 36-37 0.6023025615345965 1103 38-39 0.6589641143344238 1103 40-41 1.0399673503052753 1103 42-43 0.9761257523161078 1103 44-45 -2.7103379851750518 1103 46-47 0.3661701610377328 1103 48-49 0.3603180852126293 1103 50-51 0.48380838748914456 1103 52-53 -0.043473376603476055 1103 54-55 1.3916953467649975 1103 56-57 0.8021459546066296 1103 58-59 1.0878846518357754 1103 60-61 0.9266738096960729 1103 62-63 1.2515437204634736 1103 64-65 1.0177005302587432 1103 66-67 0.48537272753273797 1103 68-69 -2.3822634529449545 1103 70-71 -0.2423190708284899 1103 72-73 0.5426817764125396 1103 74-75 -0.8096364338609554 1103 76 0.5467487968540325 1104 1 -1.891451854024936 1104 2 -1.0553623554361522 1104 3 -1.4256348669128656 1104 4 -1.5269183403376765 1104 5 -1.8803985436859136 1104 6 -2.2767919569249955 1104 7 -1.1122281235898441 1104 8 -0.8417552396629198 1104 9 -1.2574258537334622 1104 10-11 -1.391511729272164 1104 12-13 -1.0085497975205904 1104 14-15 -0.7410709495415766 1104 16-17 -0.79424492479815 1104 18-19 -0.7129100736581542 1104 20-21 -0.701233095045481 1104 22-23 -0.8675502300066675 1104 24-25 -0.08165257576443352 1104 26-27 -0.22242099757734124 1104 28-29 -0.7924904001137705 1104 30-31 -1.4678164497624522 1104 32-33 -1.954950747095193 1104 34-35 -1.1912949406985973 1104 36-37 -1.2223174992556771 1104 38-39 -1.127307415554128 1104 40-41 -0.36763143388925457 1104 42-43 -0.13033321424924083 1104 44-45 -4.134172838265226 1104 46-47 -1.4201266981314689 1104 48-49 0.3618885006128352 1104 50-51 -0.4879241353072068 1104 52-53 -0.6193852307068219 1104 54-55 -1.5356602763353067 1104 56-57 -1.2302248660620663 1104 58-59 -0.5353169692381883 1104 60-61 -1.201948277436653 1104 62-63 -0.7644137263450368 1104 64-65 -1.1948881222235244 1104 66-67 -1.0525198763173123 1104 68-69 -3.2857335643937873 1104 70-71 -1.3736513909905952 1104 72-73 -0.18363534618118038 1104 74-75 -1.4123466668903362 1104 76 -0.3670809903800105 1105 1 0.023173996315197343 1105 2 -0.350260314619824 1105 3 -0.7164511934434792 1105 4 -0.8412040546233932 1105 5 -0.017133237563466963 1105 6 -0.3723701882175128 1105 7 0.2738262981788644 1105 8 0.32967333176565106 1105 9 0.417744214293748 1105 10-11 -0.01872261362590777 1105 12-13 -1.6352504777926988 1105 14-15 -0.5618192488613047 1105 16-17 -0.043224516634882804 1105 18-19 -0.37974680835203145 1105 20-21 -0.6343963603516052 1105 22-23 -0.9607475089182316 1105 24-25 -0.32246890229504643 1105 26-27 -0.7782033105025086 1105 28-29 -1.229395162018534 1105 30-31 -0.7188368579257229 1105 32-33 0.3341648991633086 1105 34-35 -0.27088677743329015 1105 36-37 0.664757330676295 1105 38-39 0.07371299260913844 1105 40-41 -0.3928015019164661 1105 42-43 0.03684365649905885 1105 44-45 0.6571536923470198 1105 46-47 0.14160799574608163 1105 48-49 -0.5296080980266176 1105 50-51 -0.3715976046949656 1105 52-53 -0.6369022375095419 1105 54-55 -0.6458643579679588 1105 56-57 -0.2951908524566207 1105 58-59 -0.14348023454430603 1105 60-61 -0.13085984206250245 1105 62-63 0.28490600154612267 1105 64-65 0.12705065328668042 1105 66-67 -0.5089824613513265 1105 68-69 -0.06651587731895958 1105 70-71 0.698627520574032 1105 72-73 0.18286125245827378 1105 74-75 -0.6478908845774107 1105 76 -1.3470129631691243 1106 1 -0.24198095455403745 1106 2 0.18670113662734167 1106 3 -1.4385978298758282 1106 4 -0.8676590810784184 1106 5 -1.3137318770192472 1106 6 -0.8839348140678531 1106 7 0.6361845748228525 1106 8 0.557186559278879 1106 9 0.07775933145172331 1106 10-11 0.6449962072357742 1106 12-13 0.12068300671221266 1106 14-15 0.7237438652732386 1106 16-17 0.7697762392230132 1106 18-19 0.25494706919898746 1106 20-21 0.9081584393460531 1106 22-23 0.43350797105153305 1106 24-25 0.4395114453995852 1106 26-27 -0.535119410275751 1106 28-29 -0.4950036276269998 1106 30-31 -0.38633496828097336 1106 32-33 0.5603931682487193 1106 34-35 1.2229907735871137 1106 36-37 0.9454073684691942 1106 38-39 -0.2185772568239699 1106 40-41 0.18898232272450244 1106 42-43 0.4951958862798591 1106 44-45 0.6635784844860986 1106 46-47 0.3366193336145642 1106 48-49 0.4700895588138927 1106 50-51 0.5522875049044309 1106 52-53 0.491593605272012 1106 54-55 0.5585196178445884 1106 56-57 0.38591270007550094 1106 58-59 -0.06018469410591365 1106 60-61 0.45228452679615216 1106 62-63 0.2633640514327418 1106 64-65 0.5023340999986985 1106 66-67 0.9014483776509401 1106 68-69 0.5331817795215557 1106 70-71 -0.1780164703556757 1106 72-73 -0.3210692086150466 1106 74-75 -1.6265001060437747 1106 76 -0.7318958051948243 1107 1 0.060945748372663644 1107 2 -0.803664053737851 1107 3 -0.32598451726251554 1107 4 0.264690051270712 1107 5 -0.27830064158801093 1107 6 -1.046172576305615 1107 7 -0.5095807709424918 1107 8 0.4665364686287887 1107 9 -0.5214118677194755 1107 10-11 -0.5569462947067301 1107 12-13 -0.9230103369811324 1107 14-15 -0.8658961243667491 1107 16-17 -0.8444946750478977 1107 18-19 -0.5821158678639478 1107 20-21 -0.35410522291761026 1107 22-23 -1.0045132669697026 1107 24-25 -0.5104238045356659 1107 26-27 -0.4313121064684502 1107 28-29 -0.5064514390748087 1107 30-31 -0.18879547074147496 1107 32-33 -0.0049007970452414895 1107 34-35 -0.20288335228701015 1107 36-37 -0.968371445309625 1107 38-39 -0.5951895334362476 1107 40-41 -0.256342722600543 1107 42-43 -0.4762622851783114 1107 44-45 -0.8245075035998894 1107 46-47 -0.5103115133162852 1107 48-49 -0.01688272815839298 1107 50-51 0.04384409646102583 1107 52-53 -0.1920375783591659 1107 54-55 0.39281125213622303 1107 56-57 -0.45614894198613953 1107 58-59 -0.11843385235506787 1107 60-61 -0.6151850406734134 1107 62-63 -0.3745535864848968 1107 64-65 0.3430489407135404 1107 66-67 0.538589014791576 1107 68-69 -0.2950492487094749 1107 70-71 -0.49915089149009617 1107 72-73 -0.7344595220053556 1107 74-75 -0.08404996359363182 1107 76 -0.7618362351352523 1108 1 -1.5493883619614444 1108 2 -0.7313544189282162 1108 3 -0.9256348669128656 1108 4 -1.3421961181154565 1108 5 -0.9651207659081358 1108 6 -1.5339348140678517 1108 7 -1.5110376473993696 1108 8 -1.0145726999803806 1108 9 -2.0421480759556836 1108 10-11 -1.1800037927642286 1108 12-13 -1.2158910673618628 1108 14-15 -0.3334320606526866 1108 16-17 -2.4255941311473563 1108 18-19 -1.4679695974676754 1108 20-21 -1.071471190283578 1108 22-23 -2.5662605474669853 1108 24-25 -1.5753033694152272 1108 26-27 -1.6483138547201968 1108 28-29 -1.4218554794788503 1108 30-31 -1.8518442275402336 1108 32-33 -1.8185420169364654 1108 34-35 -0.9242314486351049 1108 36-37 -1.7738055944937692 1108 38-39 -1.7317717012684142 1108 40-41 -1.4170361957940152 1108 42-43 -1.4286467063127333 1108 44-45 -0.8977641081064966 1108 46-47 -1.5487973330521037 1108 48-49 -1.3074567374824042 1108 50-51 -0.9979439765770515 1108 52-53 -1.2977582465798356 1108 54-55 -1.2264340858591112 1108 56-57 -0.8812169295541281 1108 58-59 -2.254166175587393 1108 60-61 -1.0488728806112562 1108 62-63 -1.7505248374561475 1108 64-65 -0.5428047888901872 1108 66-67 -0.0048016223490598975 1108 68-69 -0.3651978501080748 1108 70-71 -0.11968313702234212 1108 72-73 -1.7729210604668957 1108 74-75 -1.1628426986363678 1108 76 -0.28791432371334125 1109 1 -0.5452869451083515 1109 2 -0.6058417418215782 1109 3 0.04248593845626303 1109 4 -0.8584619644987512 1109 5 0.27597729524025993 1109 6 -0.3913173644034238 1109 7 -0.4978944706655888 1109 8 0.1868161889085087 1109 9 0.6335137435819753 1109 10-11 1.1623713004497915 1109 12-13 1.1538423405426848 1109 14-15 -0.15415819786372253 1109 16-17 -0.44899085001387107 1109 18-19 -0.12827440731107842 1109 20-21 0.5700276911481907 1109 22-23 1.0669655897440506 1109 24-25 0.8381940205922298 1109 26-27 0.41104017212544264 1109 28-29 -0.052821176719717755 1109 30-31 0.5234587180227805 1109 32-33 0.7032467600955989 1109 34-35 0.18473574003007087 1109 36-37 0.5289768439849745 1109 38-39 0.7168440705436652 1109 40-41 0.21453539257287346 1109 42-43 1.0448106016663878 1109 44-45 0.65338708503295 1109 46-47 0.6893411829807086 1109 48-49 0.0699901305265449 1109 50-51 0.21763674676374123 1109 52-53 0.7217700904820568 1109 54-55 0.6107443257739966 1109 56-57 0.5387588348008308 1109 58-59 0.4065814008481041 1109 60-61 0.7290680217004528 1109 62-63 -0.3097157397678565 1109 64-65 -0.40445188061278614 1109 66-67 0.4346325685532548 1109 68-69 0.6807333579456163 1109 70-71 -0.12089491927439155 1109 72-73 0.6599648455733735 1109 74-75 1.3449639754874205 1109 76 -0.13453065480953796 1110 1 0.8105150196810662 1110 2 0.8335610400089841 1110 3 0.6576984664204666 1110 4 0.7281541234304392 1110 5 0.07322464471988255 1110 6 0.32476083810606227 1110 7 1.6067159757890401 1110 8 2.5853669135461885 1110 9 0.3404002332230611 1110 10-11 0.12494789805702666 1110 12-13 0.2499905751502176 1110 14-15 0.7835667316178458 1110 16-17 1.4735604582246253 1110 18-19 1.4890050402134811 1110 20-21 0.7546157662381603 1110 22-23 1.2415595008421931 1110 24-25 0.8027159542562678 1110 26-27 -0.3607232750100522 1110 28-29 1.142758047091199 1110 30-31 0.8502994922665295 1110 32-33 1.8669954226770606 1110 34-35 1.6005270054711787 1110 36-37 1.2991110721728951 1110 38-39 1.9253840958330386 1110 40-41 1.4661159781190278 1110 42-43 2.420236143928811 1110 44-45 2.141034201072248 1110 46-47 1.0962811693633583 1110 48-49 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42-43 -0.3906112949471705 2116 44-45 1.5845567146246324 2116 46-47 0.8713945668630778 2116 48-49 -0.06946373493787306 2116 50-51 0.873689823253315 2116 52-53 0.855178564276823 2116 54-55 1.072627618975492 2116 56-57 0.6432211535671613 2116 58-59 0.14908870142702568 2116 60-61 -0.40592864821091723 2116 62-63 0.764212227853438 2116 64-65 0.37171493121159216 2116 66-67 -0.7859986113227677 2116 68-69 0.8172926079071914 2116 70-71 -0.5351835611105535 2116 72-73 0.6431094738842482 2116 74-75 -0.3386007563123634 2116 76 -0.8147909140441314 2117 1 -0.08280523748358348 2117 2 0.2780962911803897 2117 3 1.1213576142901402 2117 4 0.15127714838412487 2117 5 -1.428518844437793 2117 6 0.061929847586284836 2117 7 -0.4787694769733015 2117 8 -0.22671764567795805 2117 9 0.3722733943868377 2117 10-11 0.45961609027670747 2117 12-13 0.3156983227801611 2117 14-15 0.6397561181276004 2117 16-17 1.6651535714424526 2117 18-19 0.5880297759659037 2117 20-21 0.8602330703680536 2117 22-23 1.6452317248805528 2117 24-25 1.4927835144611272 2117 26-27 1.4670526866331848 2117 28-29 0.8031862916155532 2117 30-31 0.7494767833202545 2117 32-33 1.0600868468897708 2117 34-35 0.07712611193298358 2117 36-37 0.4814418992405649 2117 38-39 0.876076043092489 2117 40-41 -0.10296977975391641 2117 42-43 -0.5143557706402184 2117 44-45 0.6265414474490605 2117 46-47 0.575549993597857 2117 48-49 0.9976027863271177 2117 50-51 1.7256096992792571 2117 52-53 1.5052388294435524 2117 54-55 -0.06423170490673158 2117 56-57 -0.12458576831770074 2117 58-59 0.20453265482196414 2117 60-61 1.5315103691798875 2117 62-63 0.963029882677521 2117 64-65 0.43048781762610133 2117 66-67 -0.4502642372195709 2117 68-69 0.7952814732001983 2117 70-71 1.1366869548740688 2117 72-73 1.4977556312624252 2117 74-75 1.1900969421322216 2117 76 0.46788141563502705 2118 1 0.40246348989040825 2118 2 1.8311455810717838 2118 3 1.3021429108649123 2118 4 1.1101186966993595 2118 5 0.9603421970548247 2118 6 1.0716207414877026 2118 7 0.8435919822302616 2118 8 -2.1909615888692677 2118 9 -0.8629814092890165 2118 10-11 0.06721842945799139 2118 12-13 1.4021644881936943 2118 14-15 -0.5614413199119497 2118 16-17 0.8882947577415337 2118 18-19 0.3808729951249141 2118 20-21 -2.5696193384317247 2118 22-23 0.20017463771819877 2118 24-25 0.5432151491032933 2118 26-27 1.1648805897242482 2118 28-29 0.41240377978040854 2118 30-31 0.5173687354227283 2118 32-33 -0.2766438687883159 2118 34-35 1.5341018846982308 2118 36-37 0.7713332943951166 2118 38-39 0.14808940984269725 2118 40-41 -0.3332398994977197 2118 42-43 0.31741810850208196 2118 44-45 0.3339488548564695 2118 46-47 -0.23004733305210223 2118 48-49 0.10342289214722555 2118 50-51 -0.6403050876881586 2118 52-53 -0.7454434317650218 2118 54-55 0.4177788771038493 2118 56-57 1.8785052926680947 2118 58-59 1.3620375281163106 2118 60-61 -1.1588265843149586 2118 62-63 1.0448455329142199 2118 64-65 -0.09396219629759628 2118 66-67 -1.5133664371638744 2118 68-69 0.5665151128548871 2118 70-71 0.31087241853321146 2118 72-73 -0.28403217157800853 2118 74-75 0.28090730136363007 2118 76 -0.14671062000963886 2119 1 -0.20323451580759766 2119 2 0.1986669485931536 2119 3 0.5038523125743133 2119 4 0.06966285624351798 2119 5 0.36661000332263427 2119 6 0.6305951004620631 2119 7 -1.7108239721856933 2119 8 -0.5738675717752528 2119 9 -0.5074258537334622 2119 10-11 -0.23449097225140747 2119 12-13 -0.18330559727639084 2119 14-15 -0.3107290691996951 2119 16-17 -1.0997394302926544 2119 18-19 -0.2818050675531474 2119 20-21 -0.6206164894288762 2119 22-23 0.5141347516783128 2119 24-25 0.07426928015742007 2119 26-27 0.5512053760490367 2119 28-29 0.3591274265040525 2119 30-31 -0.6122608942068979 2119 32-33 0.40341311126866586 2119 34-35 0.7306830812794232 2119 36-37 -0.7329402098783895 2119 38-39 -1.4809704192171296 2119 40-41 -1.0457755120333303 2119 42-43 -1.320473629389653 2119 44-45 -0.58286026195265 2119 46-47 -0.3044063074110781 2119 48-49 -0.9791981904738556 2119 50-51 0.1075581601750848 2119 52-53 -0.5696599559815461 2119 54-55 -0.846893487568515 2119 56-57 0.3511548653176675 2119 58-59 1.113889379968164 2119 60-61 -0.3140972395856174 2119 62-63 -0.3263795383108494 2119 64-65 -0.3010847034201021 2119 66-67 -0.10282512662256238 2119 68-69 -0.14858460224482428 2119 70-71 -1.2617771541163556 2119 72-73 -1.1917244792703166 2119 74-75 -0.5712294507731208 2119 76 0.11539764209862469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 12.0 13 163.0 14 589.0 15 1163.0 16 1535.0 17 1922.0 18 1996.0 19 1915.0 20 1654.0 21 1378.0 22 965.0 23 753.0 24 710.0 25 586.0 26 720.0 27 680.0 28 835.0 29 969.0 30 1145.0 31 1379.0 32 1753.0 33 2412.0 34 3245.0 35 4742.0 36 8111.0 37 9618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.569584214792698 31.30350540738251 10.087190335910162 29.03972004191463 2 15.013541625780116 22.95011186560427 31.877772108176 30.158574400439615 3 13.849746830474546 32.61176747654748 27.153903520822702 26.38458217215528 4 10.362287553479609 29.524669309573344 32.10542842563881 28.007614711308236 5 10.175844879695411 41.61400478863288 30.627624916591433 17.58252541508027 6 20.795227067551124 37.82234957020057 16.165561094320367 25.216862267927937 7 22.027711268987716 33.981630490246104 20.182910075754602 23.80774816501158 8 21.03465871177925 36.63304156690348 18.32633355575617 24.005966165561095 9 20.90905522628253 21.45268281194803 19.490128351061742 38.148133610707696 10-11 20.533226047022804 27.96934489932096 25.509282882599994 25.988146171056247 12-13 19.786474074655573 25.570122070887468 25.357184911881305 29.28621894257566 14-15 19.65792675746752 27.153903520822702 22.055187031440123 31.132982690269657 16-17 20.80994622600777 28.574792950504374 23.694901283510617 26.920359539977234 18-19 19.38022530125211 27.60627232405699 24.027554264630844 28.985948110060058 20-21 18.729638497468304 28.144012246339834 24.256191859324097 28.870157396867764 22-23 21.891313733956117 27.365859402598424 23.353416807316403 27.38941005612906 24-25 20.04749381795345 29.784707775640772 23.230757153511007 26.937041252894765 26-27 18.251756486242492 30.419594143737488 25.148172861796915 26.180476508223105 28-29 20.464536640891783 28.118499038348315 26.21972759744083 25.197236723319072 30-31 20.29085057110335 27.711268987714412 26.85755779722887 25.140322643953372 32-33 18.30965184283864 30.19488165796601 26.28351061741963 25.211955881775722 34-35 19.24284648899007 29.114495427248105 25.896887388625032 25.745770695136788 36-37 19.599050123640932 28.13910586018762 25.875299289555286 26.38654472661616 38-39 19.392000628017428 26.512148212112884 27.94677552302076 26.149075636848924 40-41 18.511794952309927 26.492522667504026 27.396278996742158 27.59940338344389 42-43 17.1625387604506 26.151038191309812 28.689602386466223 27.996820661773363 44-45 17.1056246810849 26.583781449935234 29.30584448718452 27.004749381795346 46-47 17.60215095968913 27.345252580759116 28.241158692153707 26.811437767398044 48-49 16.910350512226717 28.18915099894022 27.410998155198808 27.489500333634258 50-51 16.71605762059897 28.182282058327118 26.551399301330612 28.5502610197433 52-53 16.293127134277977 27.844722691054674 27.188248223888213 28.673901950779136 54-55 16.17831769831613 27.789771166149862 26.7103662126624 29.321544922871613 56-57 17.094830631550025 28.062566236213055 25.954782745221177 28.88782038701574 58-59 17.101699572163128 27.18137928327511 26.08921772579189 29.62770341876987 60-61 16.60321073909801 26.64462063822271 26.09510538917455 30.65706323350473 62-63 16.86521175962633 27.438473917651212 25.560309298583032 30.13600502413942 64-65 17.468697256348864 28.052753463908626 23.950033363425835 30.528515916316678 66-67 16.911331789457158 29.722887310122857 23.169917965223537 30.19586293519645 68-69 17.32052439455195 29.13215841739608 23.14047964831024 30.406837539741726 70-71 16.930957334066022 30.82584291714095 22.624327825097147 29.618871923695885 72-73 18.204655179181223 30.093810103230368 22.27597440828983 29.425560309298582 74-75 17.93284138634847 30.155630568748283 23.1964124504455 28.715115594457746 76 18.626604388271776 28.694508772618438 23.866624798838167 28.81226204027162 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 4.0 12 6.0 13 3.5 14 2.5 15 4.0 16 7.5 17 11.0 18 14.0 19 17.0 20 22.0 21 27.0 22 41.5 23 57.0 24 73.0 25 88.0 26 123.5 27 177.0 28 240.0 29 285.0 30 349.0 31 491.5 32 663.5 33 757.0 34 847.5 35 1060.5 36 1353.5 37 1524.0 38 1667.5 39 2111.5 40 2581.0 41 2889.0 42 3028.0 43 3167.0 44 3399.0 45 3578.5 46 3665.0 47 3642.5 48 3527.5 49 3235.0 50 3035.0 51 2881.5 52 2554.0 53 2182.0 54 1984.0 55 1848.0 56 1518.0 57 1178.0 58 1032.0 59 897.0 60 665.0 61 520.5 62 473.0 63 396.0 64 272.0 65 194.0 66 140.0 67 117.0 68 95.5 69 55.5 70 37.0 71 37.0 72 32.5 73 25.5 74 16.5 75 10.0 76 11.0 77 8.5 78 4.5 79 4.0 80 6.0 81 7.0 82 6.0 83 6.0 84 6.0 85 4.5 86 4.0 87 5.0 88 5.0 89 6.5 90 4.0 91 1.5 92 3.0 93 1.5 94 2.0 95 4.0 96 4.0 97 3.0 98 2.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7359579228323586 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 50954.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.15209797071869 #Duplication Level Percentage of deduplicated Percentage of total 1 83.99446374010915 63.12360167994662 2 8.502859530462487 12.780154649291516 3 3.692580889457603 8.32515602307964 4 1.6269292037709242 4.890685716528634 5 0.9087822839683494 3.4148447619421436 6 0.5066200088789075 2.284413392471641 7 0.28203588123155665 1.483691172430035 8 0.1592980440289348 0.9577265769125093 9 0.09662340375525554 0.6535306354751345 >10 0.22980701433682396 2.086195391922126 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019625544608862897 0.0 2 0.0 0.0 0.0 0.0019625544608862897 0.0 3 0.0 0.0 0.0 0.0019625544608862897 0.0 4 0.0 0.0 0.0 0.0019625544608862897 0.0 5 0.0 0.0 0.0 0.003925108921772579 0.0 6 0.0 0.0 0.0 0.005887663382658869 0.0 7 0.0 0.0 0.0 0.005887663382658869 0.0 8 0.0 0.0 0.0 0.005887663382658869 0.0 9 0.0 0.0 0.0 0.009812772304431448 0.0 10 0.0 0.0 0.0 0.009812772304431448 0.0 11 0.0 0.0 0.0 0.017662990147976607 0.0 12 0.0 0.0 0.0 0.017662990147976607 0.0 13 0.0 0.0 0.0 0.017662990147976607 0.0 14 0.0 0.0 0.0 0.019625544608862896 0.0 15 0.0 0.0 0.0 0.023550653530635474 0.0 16 0.0 0.0 0.0 0.025513207991521764 0.0 17 0.0 0.0 0.0 0.025513207991521764 0.0 18 0.0 0.0 0.0 0.027475762452408053 0.0 19 0.0 0.0 0.0 0.03336342583506692 0.0 20 0.0 0.0 0.0 0.04906386152215724 0.0 21 0.0 0.0 0.0 0.05298897044392982 0.0 22 0.0 0.0 0.0 0.054951524904816106 0.0 23 0.0 0.0 0.0 0.0569140793657024 0.0 24 0.0 0.0 0.0 0.0569140793657024 0.0 25 0.0 0.0 0.0 0.0569140793657024 0.0 26 0.0 0.0 0.0 0.06672685167013384 0.0 27 0.0 0.0 0.0 0.08242728735722417 0.0 28 0.0 0.0 0.0 0.11579071319229109 0.0 29 0.0 0.0 0.0 0.13345370334026768 0.0 30 0.0 0.0 0.0 0.1687796836362209 0.0 31 0.0 0.0 0.0 0.2276563174628096 0.0 32 0.0 0.0 0.0 0.2806452879067394 0.0 33 0.0 0.0 0.0 0.33363425835066923 0.0 34 0.0 0.0 0.0 0.3826981198728265 0.0 35 0.0 0.0 0.0 0.4867135062997998 0.0 36 0.0 0.0 0.0 0.671193625623111 0.0 37 0.0 0.0 0.0 0.9636142402951682 0.0 38 0.0 0.0 0.0 1.3247242610982455 0.0 39 0.0 0.0 0.0 1.6681712917533462 0.0 40 0.0 0.0 0.0 2.1725477882011224 0.0 41 0.0 0.0 0.0 2.7318758095537152 0.0 42 0.0 0.0 0.0 3.3500804647328963 0.0 43 0.0 0.0 0.0 4.033049417121325 0.0 44 0.0 0.0 0.0 4.6806923892138 0.0 45 0.0 0.0 0.0 5.345998351454253 0.0 46 0.0 0.0 0.0 5.972053224476979 0.0 47 0.0 0.0 0.0 6.576519998429957 0.0 48 0.0 0.0 0.0 7.100522039486596 0.0 49 0.0 0.0 0.0 7.65396239745653 0.0 50 0.0 0.0 0.0 8.217215527730895 0.0 51 0.0 0.0 0.0 8.774580994622601 0.0 52 0.0 0.0 0.0 9.39867331318444 0.0 53 0.0 0.0 0.0 10.042391176355144 0.0 54 0.0 0.0 0.0 10.601719197707736 0.0 55 0.0 0.0 0.0 11.159084664599442 0.0 56 0.0 0.0 0.0 11.683086705656082 0.0 57 0.0 0.0 0.0 12.193350865486517 0.0 58 0.0 0.0 0.0 12.73109078776936 0.0 59 0.0 0.0 0.0 13.251167719904227 0.0 60 0.0 0.0 0.0 13.708442909290733 0.0 61 0.0 0.0 0.0 14.214781960199396 0.0 62 0.0 0.0 0.0 14.691682694194764 0.0 63 0.0 0.0 0.0 15.16465831926836 0.0 64 0.0 0.0 0.0 15.700435687090316 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTTG 20 0.006756569 52.80652 1 TTAATGC 20 0.0069164564 52.495586 28 GATCCAA 20 0.0069164564 52.495586 14 TATAGTG 35 0.0012450244 39.996635 5 TTATCCA 45 9.083178E-5 38.88562 2 TAATACT 40 0.0023988606 34.997055 4 GCCACCC 40 0.0023988606 34.997055 31 TATCCAC 55 2.955225E-4 31.815508 3 AATAACA 45 0.0042720553 31.108496 25 AGATCTC 60 4.920962E-4 29.164211 40 ATTAAAA 60 4.920962E-4 29.164211 59 ATCTCGG 50 0.0071498216 27.997644 42 TCTCGGT 50 0.0071498216 27.997644 43 GATCTCG 50 0.0071498216 27.997644 41 TATAACA 50 0.0071498216 27.997644 3 TGTAGAT 80 8.517045E-5 26.247793 37 CGGTGGT 70 0.0012111287 24.997896 46 GTAGATC 85 1.2859277E-4 24.703804 38 CTTATCC 75 0.0017488111 23.469563 1 GTGTAGA 80 0.0026339458 21.873161 36 >>END_MODULE