FastQCFastQC Report
Wed 25 May 2016
SRR1780478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50954
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT33036.482317384307415No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT29515.791498214075441Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC9871.9370412528947678No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC4670.9165129332338972No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG3200.6280174274836127No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT2140.419986654629666No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1780.3493346940377595No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1370.26886996114142164No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1330.2610197432978765No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1300.25513207991521764No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG990.1942928916277427No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC970.1903677827059701No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC960.18840522824508382No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT930.18251756486242493No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT740.14522903010558544No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT720.14130392118381285No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC630.12364093103583625No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGTCTT580.1138281587314048No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT580.1138281587314048No Hit
CTTATCACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC550.10794049534874593No Hit
CTTATTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG540.10597794088785964No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA150.002212687670.041
GTCCTCT207.8562356E-570.046
TATGCCC150.002212687670.043
ATCGTTG150.002212687670.040
CGTCCTC150.002212687670.045
GCCGTCC150.002212687670.043
GTAAATG150.002212687670.033
CCTCTGC207.8562356E-570.048
TTTACAC1200.058.3333322
CTTTACA1200.058.3333321
CTATACA1450.057.9310381
GTCCTGA200.006914157452.528
CGAGGTC200.006914157452.543
CGATGGG200.006914157452.560
ATAACAC200.006914157452.54
ATGCCCT200.006914157452.544
TCCTCTG305.82637E-446.66666447
CTTATAA305.82637E-446.6666641
TCTTTAT305.82637E-446.6666641
TTACACA1600.045.93753
Produced by FastQC (version 0.11.5)