FastQCFastQC Report
Wed 25 May 2016
SRR1780477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471763
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA407.0149323E-427.5072324
TGAGCGT508.33999E-526.39573522
ACGAGAG1300.025.39128924
GTAAACA1051.1277734E-1025.14146236
CTAGAGA450.001406063124.417184
TAAACAG1102.0008883E-1023.99866737
GGCATAG651.814102E-523.6859651
GCGCAAT752.0475381E-623.4828111
TCGGTGG2700.022.79178442
CGGTGGT1550.022.68675243
AAGGCAA500.002544183322.05025516
CTCGCTT703.1669013E-522.03853618
CGCAATC803.5632656E-622.03150712
AAACAGG1205.966285E-1021.99878138
GAGCGTC500.00258054321.99644723
CTATACA1800.021.9941121
AAACTCG1105.2696123E-921.98711427
TACCACT500.002594886621.9754624
CCTGTAC500.002594886621.9754623
CGTCAGA602.8929918E-421.9731312
Produced by FastQC (version 0.11.5)