FastQCFastQC Report
Wed 25 May 2016
SRR1780477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471763
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT390078.268346606240845No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT302026.4019433486729564RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC94442.001852625152884No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC46080.9767616366692597No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG43340.9186816261555061No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT25900.5490044789438765No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC23900.5066103106856621No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC16400.34763217971735805No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG14300.30311830304623294No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG9830.20836733698912377No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC9510.2015842700678095No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC8750.185474486129688No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC5960.1263346214094789No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5080.10768118737586457No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG5050.10704527485199135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGT207.84654E-444.0059412
CCGTCTT29750.042.73956344
GCCGTCT33400.038.0689243
TTTACAC10700.037.6272542
CTTATAA1550.036.9316981
CTTTACA11200.036.1707421
ATCTAGT250.002342462535.2271581
TCGGGTT250.00234978735.2047513
GCACGAC250.002351009535.2010221
CGACAAC250.002351009535.2010224
GATTCCC250.002352232635.1972937
CTATACA6400.034.401521
TTACACA12500.032.208933
CCTTATA353.2004013E-431.4528181
TTATACA56950.030.5575122
CTTATAC58250.029.973321
GTCATAC300.00571990729.3559651
TCTTTAT1950.029.3559651
GATGAGC300.00573772429.33729216
TGTCTTC300.005740697529.33418326
Produced by FastQC (version 0.11.5)