FastQCFastQC Report
Wed 25 May 2016
SRR1780476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG160.1313521057384451No Hit
ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC140.11493309252113948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCC207.3138806E-570.572621
CTTTACA200.00657187752.9294621
TTACACA400.002328098334.9958843
TACCCCC400.002328098334.99588412
ACATCTG800.002521452321.8724278
Produced by FastQC (version 0.11.5)