##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12181 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.371151793777194 23.0 12.0 31.0 12.0 32.0 2 20.036696494540678 21.0 11.0 27.0 11.0 33.0 3 18.25843526804039 11.0 11.0 23.0 11.0 32.0 4 20.164764797635662 21.0 11.0 31.0 11.0 33.0 5 19.224447910680567 21.0 11.0 27.0 11.0 33.0 6 21.84081766685822 21.0 11.0 31.0 11.0 36.0 7 22.61858632296199 23.0 11.0 34.0 11.0 36.0 8 22.53370002462852 23.0 11.0 34.0 11.0 36.0 9 22.206879566538053 21.0 12.0 33.0 12.0 36.0 10-11 20.728060093588375 21.0 11.0 31.0 11.0 35.0 12-13 20.61480994992201 21.0 11.0 29.5 11.0 34.0 14-15 20.258640505705607 21.0 11.0 28.0 11.0 34.0 16-17 19.598144651506445 11.5 11.0 27.5 11.0 34.0 18-19 20.098637221902962 21.0 11.0 27.5 11.0 34.0 20-21 20.271242098349887 16.0 11.0 29.0 11.0 34.0 22-23 19.470486823741894 16.0 11.0 27.0 10.5 34.0 24-25 19.637057712831457 16.0 11.0 27.0 10.5 34.0 26-27 19.311058205401856 16.0 11.0 26.0 10.0 34.0 28-29 18.809046876282736 11.0 11.0 26.0 11.0 34.0 30-31 18.694195878827685 11.0 11.0 26.0 11.0 34.0 32-33 19.121418602741976 11.0 11.0 26.0 11.0 34.0 34-35 19.421229784089974 16.0 11.0 26.5 11.0 34.0 36-37 19.178228388473855 11.0 11.0 26.0 11.0 34.0 38-39 18.53612182907807 11.0 11.0 26.0 11.0 34.0 40-41 18.920942451358673 11.0 11.0 26.0 11.0 34.0 42-43 19.182415236844264 11.0 11.0 26.0 11.0 34.0 44-45 18.996593054757412 11.0 11.0 26.0 11.0 34.0 46-47 19.22600771693621 16.0 11.0 26.0 11.0 34.0 48-49 19.663287086446104 21.0 11.0 27.0 11.0 34.0 50-51 19.582751826615223 21.0 11.0 27.0 11.0 34.0 52-53 19.44150726541335 16.0 11.0 26.0 11.0 34.0 54-55 19.506239225022576 20.5 11.0 26.0 11.0 34.0 56-57 18.821402183728758 15.0 11.0 26.0 11.0 33.5 58-59 18.815942861834003 16.0 11.0 26.0 11.0 33.5 60-61 19.152163204991382 15.0 11.0 26.0 11.0 33.0 62-63 18.741441589360477 15.0 11.0 26.0 11.0 33.0 64-65 19.08246449388392 15.0 11.0 26.0 11.0 33.0 66-67 19.351490025449472 19.0 11.0 26.0 11.0 33.0 68-69 19.144241031114028 19.0 11.0 26.0 10.5 33.0 70-71 19.60791396437074 19.0 11.0 26.0 11.0 33.0 72-73 18.79615795090715 14.0 11.0 25.0 11.0 33.0 74-75 18.38761185452754 11.0 11.0 24.5 11.0 32.5 76 17.432558903209916 11.0 11.0 24.0 11.0 31.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.261135294093581 1101 2 0.05591890577207792 1101 3 0.6786569121900747 1101 4 3.4517439278220756 1101 5 -3.2563167804192066 1101 6 -1.0810698321164764 1101 7 -2.0623018561338604 1101 8 -3.5247391757216846 1101 9 3.2283146792529003 1101 10-11 -0.2528861239484286 1101 12-13 0.6298806036272495 1101 14-15 0.846125748234396 1101 16-17 -0.6307215388044156 1101 18-19 -1.5451900373472078 1101 20-21 -1.36863414824127 1101 22-23 -1.0906244191247652 1101 24-25 0.00824078690212815 1101 26-27 1.1365948804002493 1101 28-29 -1.24970620104774 1101 30-31 -0.3146632452757494 1101 32-33 -1.5037889851987458 1101 34-35 -0.7586371381114034 1101 36-37 0.33179048143758294 1101 38-39 -0.24498587505134495 1101 40-41 -0.7594991649438185 1101 42-43 0.9792183585641219 1101 44-45 -2.7393025794114116 1101 46-47 -1.1964502962776429 1101 48-49 0.013865361148305055 1101 50-51 0.16964793128717304 1101 52-53 -0.7058630160550123 1101 54-55 -0.5540002408497067 1101 56-57 0.29931150388072325 1101 58-59 -1.3157614015960135 1101 60-61 -2.485258168730571 1101 62-63 -0.7840089832621651 1101 64-65 -1.7955561264468471 1101 66-67 0.2482719476795765 1101 68-69 -2.0668910265040346 1101 70-71 0.2329881939101206 1101 72-73 -0.28322002403404767 1101 74-75 -0.3080743980356999 1101 76 -1.6537932846547498 1102 1 -0.9611352940935802 1102 2 0.04133557243874364 1102 3 -0.6046764211432603 1102 4 -0.7586727388445915 1102 5 0.4457665529141259 1102 6 -0.8935698321164764 1102 7 0.13353147719947245 1102 8 -0.1684891757216853 1102 9 -3.1633519874137654 1102 10-11 0.6564888760515686 1102 12-13 -0.28470272970608335 1102 14-15 -1.9069992517656047 1102 16-17 -0.33801320547108205 1102 18-19 -1.235815037347205 1102 20-21 -0.3613424815746029 1102 22-23 -1.4947910857914337 1102 24-25 -0.001134213097870429 1102 26-27 -2.099863452933082 1102 28-29 -0.4424145343810757 1102 30-31 -0.22924657860908226 1102 32-33 0.01600268146792061 1102 34-35 0.2517795285552644 1102 36-37 -1.0161261852290835 1102 38-39 -2.755402541718013 1102 40-41 -1.451165831610485 1102 42-43 -1.5207816414358781 1102 44-45 -1.3882609127447445 1102 46-47 -1.1516586296109743 1102 48-49 -0.23092630551835924 1102 50-51 -1.6261854020461612 1102 52-53 -0.5048213493883438 1102 54-55 -2.3352502408497067 1102 56-57 -1.1444384961192782 1102 58-59 -0.23555306826268207 1102 60-61 0.5637001646027606 1102 62-63 0.31494935007117064 1102 64-65 -1.0799311264468443 1102 66-67 -3.081936385653755 1102 68-69 -2.4273076931707003 1102 70-71 -3.0013868060898794 1102 72-73 -1.2498866907007162 1102 74-75 -1.7934910647023656 1102 76 -3.072543284654749 1103 1 0.8809699690643136 1103 2 0.9804803092808498 1103 3 0.5137446314883185 1103 4 0.6640246295764598 1103 5 1.6349112897562321 1103 6 1.9452459573572085 1103 7 0.6236630561468424 1103 8 0.5173660874362085 1103 9 0.4879638020599195 1103 10-11 1.2576401918410411 1103 12-13 0.20707358608338922 1103 14-15 -0.48019004123928966 1103 16-17 0.8815591629499728 1103 18-19 1.4705994363370074 1103 20-21 0.9059272552674997 1103 22-23 1.850603651050669 1103 24-25 -1.325969739413658 1103 26-27 -0.21252792661729458 1103 28-29 0.8906446761452393 1103 30-31 1.433582368759339 1103 32-33 -0.3844907395847095 1103 34-35 0.7369768969763193 1103 36-37 1.8572290779288103 1103 38-39 0.7988737740714598 1103 40-41 -0.24721846318943363 1103 42-43 0.7423762533009644 1103 44-45 -2.5042148601131657 1103 46-47 -0.3482046822425531 1103 48-49 -0.9554328844657292 1103 50-51 -1.458422244151425 1103 52-53 -0.015512138862028735 1103 54-55 -1.6987370829549704 1103 56-57 0.25194308282809175 1103 58-59 -0.585059647210052 1103 60-61 -0.2677143090814518 1103 62-63 -0.7664651236130418 1103 64-65 -0.7139771790784231 1103 66-67 0.37283335118835126 1103 68-69 -1.0967155879075428 1103 70-71 -0.5038539113530369 1103 72-73 0.65800804614139 1103 74-75 -0.37737264364973555 1103 76 -1.9064248636021173 1104 1 1.8245789916207045 1104 2 -0.3488429989898272 1104 3 0.1738950074281682 1104 4 1.3422201182982647 1104 5 2.3770165529141245 1104 6 0.9332158821692396 1104 7 -2.014682808514813 1104 8 -0.9818820328645437 1104 9 2.0902194411576644 1104 10-11 1.0471138760515686 1104 12-13 -0.610595586848941 1104 14-15 1.9032686053772494 1104 16-17 1.359754651671775 1104 18-19 0.11909567693851031 1104 20-21 -0.06625319586031964 1104 22-23 0.9712803427799948 1104 24-25 -1.2060449273835836 1104 26-27 -2.3538813100759377 1104 28-29 0.5955318941903549 1104 30-31 1.5377177071052053 1104 32-33 -0.41569374710350715 1104 34-35 1.2794580999838345 1104 36-37 1.1794095290566275 1104 38-39 -1.5116525417180142 1104 40-41 1.2666913112466567 1104 42-43 0.25778978713555034 1104 44-45 0.03450694439811386 1104 46-47 -0.779783629610975 1104 48-49 -0.881372734089787 1104 50-51 1.3148860265252686 1104 52-53 1.0108036506116562 1104 54-55 0.29599975915029475 1104 56-57 -2.1578313532621323 1104 58-59 -0.6609994968341084 1104 60-61 -2.116210549682954 1104 62-63 -0.1721042213574009 1104 64-65 0.2901581592674418 1104 66-67 -0.28506138565375494 1104 68-69 -1.119271978884985 1104 70-71 -0.8384403775184524 1104 72-73 -0.9713152621292842 1104 74-75 1.8681160781547774 1104 76 -2.668078998940464 1105 1 -0.3842122171705036 1105 2 0.41232916218233484 1105 3 0.35045178398494414 1105 4 2.008154184232332 1105 5 1.9513755272731004 1105 6 0.1368788858322425 1105 7 0.6351340413020381 1105 8 -2.350380201362711 1105 9 -1.28194173100351 1105 10-11 0.8291651581028496 1105 12-13 1.387572911319559 1105 14-15 1.0461257482343953 1105 16-17 -1.0179010259839032 1105 18-19 1.3407073985502294 1105 20-21 0.5941863645792438 1105 22-23 0.36578583728548963 1105 24-25 -0.673810495149155 1105 26-27 1.923774367579739 1105 28-29 2.064396363054822 1105 30-31 -1.8710735016860056 1105 32-33 -1.020455651865415 1105 34-35 0.2541833747091111 1105 36-37 1.4317904814375844 1105 38-39 0.5498859198204471 1105 40-41 1.693064937620285 1105 42-43 0.43434656369232627 1105 44-45 0.2619794718706423 1105 46-47 0.6868830370556935 1105 48-49 -0.8194679721850235 1105 50-51 0.32734023897948106 1105 52-53 0.4467010865090941 1105 54-55 1.4652305283810634 1105 56-57 0.8916191961884152 1105 58-59 1.3393668035321902 1105 60-61 0.16987003639763287 1105 62-63 0.13265768340450634 1105 64-65 1.2505977197070024 1105 66-67 2.3944257938334275 1105 68-69 -0.7822756418886492 1105 70-71 -0.24137078044885385 1105 72-73 0.5642158734018459 1105 74-75 0.8867973968360943 1105 76 0.10774517688371077 1106 1 1.5085616756033886 1106 2 -0.41983866998549857 1106 3 -0.0667976332644713 1106 4 2.024471200549346 1106 5 -1.7199531440555695 1106 6 1.7825665315198869 1106 7 -0.8077564015884064 1106 8 -2.2338300848125954 1106 9 0.7646783156165391 1106 10-11 -0.9528861239484314 1106 12-13 -0.2079981842515366 1106 14-15 -1.120540918432269 1106 16-17 0.16624815816528127 1106 18-19 0.7229917808346151 1106 20-21 1.748032518425397 1106 22-23 1.0033149748146286 1106 24-25 1.0627862414475828 1106 26-27 -0.2149202711149023 1106 28-29 0.6366574353158931 1106 30-31 -0.5237541543666566 1106 32-33 -1.5204556518654115 1106 34-35 0.12015074067647546 1106 36-37 -1.340936791289689 1106 38-39 -1.6237737538392238 1106 40-41 -0.1367718922165473 1106 42-43 -0.24047861113284696 1106 44-45 -0.6680904581992912 1106 46-47 -1.707056356883701 1106 48-49 -1.4861346388516914 1106 50-51 -0.20762479598555217 1106 52-53 1.9653491051571095 1106 54-55 0.6808482439987777 1106 56-57 -1.1491733446041295 1106 58-59 -0.9869735228081353 1106 60-61 -0.9595005929729972 1106 62-63 -1.6703726196257982 1106 64-65 -1.7773743082650242 1106 66-67 -1.2699098705022394 1106 68-69 -1.2123455719585792 1106 70-71 0.44510940603133164 1106 72-73 -2.1210988119128373 1106 74-75 1.84950135954006 1106 76 2.706812775951313 1107 1 0.8209159879577008 1107 2 -1.536388786535614 1107 3 -1.6751892416560814 1107 4 0.7773849534631019 1107 5 0.18214475804233032 1107 6 2.2650840140373703 1107 7 1.045390451558447 1107 8 -1.6067904577729664 1107 9 2.8719044228426434 1107 10-11 -0.5554502265125336 1107 12-13 -0.36883734509069654 1107 14-15 -1.67182296971432 1107 16-17 -0.31277282085569524 1107 18-19 -1.659292601449767 1107 20-21 -0.3545315841387051 1107 22-23 1.570914042413694 1107 24-25 -0.04560536694402373 1107 26-27 -0.42237947857410774 1107 28-29 -0.9356036369451779 1107 30-31 -0.49927862989113336 1107 32-33 0.9539033224935629 1107 34-35 -0.4381243175985823 1107 36-37 -1.54256849292139 1107 38-39 -0.2321653622308304 1107 40-41 -1.7941145495592004 1107 42-43 -0.20667907733331603 1107 44-45 0.9158256257167956 1107 46-47 0.6484214985941534 1107 48-49 1.1933525406354875 1107 50-51 -0.35214694050770134 1107 52-53 -2.2968886570806504 1107 54-55 -1.2911797280291957 1107 56-57 -1.249406444837227 1107 58-59 -1.0965306323652442 1107 60-61 -1.035258168730575 1107 62-63 0.8377858885327107 1107 64-65 0.6608541299634112 1107 66-67 -0.849163949756317 1107 68-69 2.0638782042651975 1107 70-71 0.656065116987044 1107 72-73 -0.7562969471109717 1107 74-75 0.24577175581045196 1107 76 1.0308220999606341 1108 1 -0.6611352940935795 1108 2 1.3940141438673166 1108 3 -2.1118192782861183 1108 4 0.28507726115540777 1108 5 -2.651554875657304 1108 6 -0.3239269749736202 1108 7 2.2424600486280433 1108 8 -0.38188203286454225 1108 9 -1.4240662731280516 1108 10-11 0.64711387605157 1108 12-13 -0.596309872563225 1108 14-15 -0.32530282319417836 1108 16-17 1.2883260802432055 1108 18-19 -0.1951900373472064 1108 20-21 0.019461089853969327 1108 22-23 0.7712803427799955 1108 24-25 0.8225265011878449 1108 26-27 0.4746901184954915 1108 28-29 1.2955318941903542 1108 30-31 0.9377177071052039 1108 32-33 1.5843062528964929 1108 34-35 2.1080295285552673 1108 36-37 0.1651238147709151 1108 38-39 0.2312046011391331 1108 40-41 1.1095484541037983 1108 42-43 -0.35649592715016354 1108 44-45 -0.8797787698876007 1108 46-47 0.1059306561033111 1108 48-49 -0.3528013055183621 1108 50-51 1.1720288836681263 1108 52-53 0.6679465077545146 1108 54-55 1.495999759150294 1108 56-57 1.1278829324521524 1108 58-59 0.7532862174516026 1108 60-61 0.04093230745990084 1108 62-63 1.6278957786425998 1108 64-65 -0.4526989835896984 1108 66-67 0.786367185774818 1108 68-69 1.0664423068292983 1108 70-71 -0.25272609180416694 1108 72-73 0.5572561664421407 1108 74-75 0.6966875067262066 1108 76 0.04620671534524945 1109 1 -1.382187925672529 1109 2 -1.8616249538770475 1109 3 -0.06520273693273282 1109 4 0.3745509453659359 1109 5 -0.3387729207700829 1109 6 -1.0547540426427915 1109 7 0.07103147719947245 1109 8 -0.7984233862480004 1109 9 -0.4857204084663955 1109 10-11 0.612903349735781 1109 12-13 -0.45082115075871343 1109 14-15 1.2566520640238679 1109 16-17 1.723664426107863 1109 18-19 -0.3320321426103625 1109 20-21 -0.7914411657851304 1109 22-23 0.3506036510506725 1109 24-25 0.7135039447968659 1109 26-27 -0.10726476872255475 1109 28-29 -0.4909342712231819 1109 30-31 0.20989815823302038 1109 32-33 0.2207724183100268 1109 34-35 -0.7630231030236807 1109 36-37 -0.8269814483869808 1109 38-39 1.2199264056504084 1109 40-41 0.2922552210210938 1109 42-43 1.6897446743535944 1109 44-45 0.49578513988683426 1109 46-47 0.7570584756521832 1109 48-49 1.1498302734290107 1109 50-51 0.13368301900646884 1109 52-53 0.22132996640112879 1109 54-55 1.064420811781872 1109 56-57 0.7387851880912493 1109 58-59 -0.2034806998416272 1109 60-61 2.508601480392233 1109 62-63 1.5098506658606432 1109 64-65 1.312338610395262 1109 66-67 0.1886228248725601 1109 68-69 0.8243370436714024 1109 70-71 -3.7012223324056706 1109 72-73 -1.0130445854375587 1109 74-75 -0.4826358015444683 1109 76 -0.8274774951810642 1110 1 0.3999758170175305 1110 2 -1.2607477608945885 1110 3 0.3119902455234076 1110 4 1.9184105944887406 1110 5 0.8103498862474581 1110 6 0.03004127899463427 1110 7 1.5154759216439153 1110 8 -1.0747391757216853 1110 9 0.9060924570306774 1110 10-11 2.9082249871626793 1110 12-13 0.1771028258494738 1110 14-15 -0.6760964739878261 1110 16-17 1.052611794528918 1110 18-19 2.0436988515416843 1110 20-21 -0.30752303713016005 1110 22-23 -0.057291085791433716 1110 24-25 -0.7667592130978704 1110 26-27 0.9782615470669178 1110 28-29 -0.09692842326996498 1110 30-31 0.8158923102798035 1110 32-33 0.10454434813458846 1110 34-35 -1.0086371381113999 1110 36-37 -0.5543206296735264 1110 38-39 -2.2727636528291235 1110 40-41 -0.8539436093882635 1110 42-43 1.1458850252307862 1110 44-45 -1.8031914683003016 1110 46-47 -1.0492280740554172 1110 48-49 -2.3527740580675527E-5 1110 50-51 -2.049796513157272 1110 52-53 -1.5169741271661223 1110 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56-57 -0.14684234227312487 2116 58-59 -0.8208896067242222 2116 60-61 1.6442290107566073 2116 62-63 1.8570166577634772 2116 64-65 1.4429054120146958 2116 66-67 2.176477075884705 2116 68-69 1.4549038452908398 2116 70-71 -1.0170118060898794 2116 72-73 -1.4229636137776396 2116 74-75 -0.22858721854851893 2116 76 2.3385144076529443 2117 1 1.47434857687416 2117 2 -1.3064466856257724 2117 3 -1.177257066304552 2117 4 -2.0439549969091075 2117 5 3.13830687549477 2117 6 1.0640914582061036 2117 7 -3.4773556195747197 2117 8 1.5462285662137987 2117 9 2.8487447867797826 2117 10-11 1.0309848437935045 2117 12-13 -1.398614020028667 2117 14-15 0.33644832887955545 2117 16-17 -0.2619043345033383 2117 18-19 -0.9500287470246249 2117 20-21 -0.2519674815746029 2117 22-23 0.9427089142085663 2117 24-25 -0.19821082600109463 2117 26-27 0.24842283738950144 2117 28-29 0.4895411107802161 2117 30-31 -0.3512223850606979 2117 32-33 -0.5258319959514317 2117 34-35 -1.5919704714447356 2117 36-37 -0.28326328200327566 2117 38-39 -1.3632654449438206 2117 40-41 -1.1568109929008052 2117 42-43 -0.7062655124036219 2117 44-45 1.1676866679004156 2117 46-47 2.2782808865180577 2117 48-49 0.5246180493203525 2117 50-51 -0.733040240755841 2117 52-53 -0.7795189300335039 2117 54-55 1.4701933075373894 2117 56-57 0.16705343936459371 2117 58-59 -0.5485571005207461 2117 60-61 1.1367848420221165 2117 62-63 3.8154533823292347 2117 64-65 0.33992774452089947 2117 66-67 1.7794547433785013 2117 68-69 -0.34968672542876433 2117 70-71 -1.6702376125414915 2117 72-73 -0.0482737874749084 2117 74-75 -0.5586120324443016 2117 76 2.7429809088936388 2118 1 -0.8611352940935788 2118 2 -1.2107477608945914 2118 3 -0.7546764211432588 2118 4 -4.014922738844591 2118 5 0.5103498862474609 2118 6 0.48559683455019 2118 7 -1.8623018561338611 2118 8 1.8085941576116475 2118 9 -0.9050186540804326 2118 10-11 -0.8028861239484328 2118 12-13 -0.15345272970608193 2118 14-15 2.262792414901064 2118 16-17 0.6026117945289187 2118 18-19 -0.9451900373472064 2118 20-21 0.6980325184253964 2118 22-23 -1.4239577524581009 2118 24-25 0.25824078690212815 2118 26-27 -1.2467384529330836 2118 28-29 0.216960465618925 2118 30-31 0.16867008805758488 2118 32-33 0.3628776814679213 2118 34-35 2.508029528555266 2118 36-37 0.33179048143758294 2118 38-39 2.505014124948655 2118 40-41 -0.5928324982771507 2118 42-43 -0.0041149747692124095 2118 44-45 0.29403075392192335 2118 46-47 0.9535497037223593 2118 48-49 -0.1194679721850278 2118 50-51 0.23631459795383591 2118 52-53 -1.9891963493883438 2118 54-55 0.26266642581695976 2118 56-57 -0.8006884961192782 2118 58-59 -1.2324280682626814 2118 60-61 0.8814084979360928 2118 62-63 -1.9340089832621636 2118 64-65 -1.9288894597801765 2118 66-67 -0.48506138565375423 2118 68-69 -0.7835576931706996 2118 70-71 -0.0170118060898794 2118 72-73 0.5834466426326195 2118 74-75 0.7752589352976358 2118 76 3.112873382011916 2119 1 -0.46113529409358023 2119 2 -2.485106735253563 2119 3 -1.1239071903740303 2119 4 -2.991845815767668 2119 5 -0.022983447085874076 2119 6 -0.1579929090395531 2119 7 -0.8905069843389875 2119 8 -2.632431483413992 2119 9 -0.23065967972145884 2119 10-11 -1.4528861239484314 2119 12-13 -0.56114503739839 2119 14-15 1.5268949790036288 2119 16-17 0.36030410222122455 2119 18-19 -1.5182669604241248 2119 20-21 1.748032518425397 2119 22-23 -0.7880603165606637 2119 24-25 0.26208694074828287 2119 26-27 -0.1851999913946223 2119 28-29 -2.0638087651503056 2119 30-31 -0.15953504014754216 2119 32-33 0.9090315276217673 2119 34-35 0.13879875932449437 2119 36-37 -1.2733377236906236 2119 38-39 1.434501304435834 2119 40-41 0.26998801454336174 2119 42-43 1.3253722047179686 2119 44-45 -0.8341743742832044 2119 46-47 -1.7682451680725144 2119 48-49 0.38566023294318086 2119 50-51 -0.7559930943538546 2119 52-53 0.7992651890731963 2119 54-55 -1.0732310100804732 2119 56-57 0.31469611926533503 2119 58-59 0.23680270096808442 2119 60-61 -2.009617143089546 2119 62-63 -1.4699064191595959 2119 64-65 1.269828488937769 2119 66-67 -1.6119844625768316 2119 68-69 -0.6220192316322404 2119 70-71 2.7137574246793505 2119 72-73 3.4616517708377437 2119 74-75 -0.747817987779289 2119 76 1.6077451768837108 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 1.0 12 16.0 13 120.0 14 444.0 15 911.0 16 1272.0 17 1626.0 18 1676.0 19 1622.0 20 1334.0 21 1016.0 22 688.0 23 411.0 24 273.0 25 191.0 26 155.0 27 95.0 28 74.0 29 57.0 30 37.0 31 33.0 32 27.0 33 26.0 34 19.0 35 24.0 36 12.0 37 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.895466930889274 27.66080422756172 7.88539344397655 58.558335397572456 2 2.323290370248748 46.75314013627781 23.717264592397996 27.206304901075445 3 18.175847631557343 30.802068795665384 21.77982103275593 29.242262540021347 4 2.3068713570314427 50.75116985469174 16.107051966176833 30.834906822099995 5 3.5136688285034072 37.353255069370334 32.82160742139397 26.311468680732286 6 5.130941630408013 32.09096133322387 10.327559313685247 52.45053772268287 7 3.4151547491995733 35.2187833511206 9.071504802561366 52.294557097118464 8 2.7583942205073475 50.948198013299404 11.46868073228799 34.82472703390526 9 8.332649207782612 34.13512847877843 10.360397340119858 47.171824973319104 10-11 6.206386996141531 32.649207782612265 22.489943354404403 38.65446186684181 12-13 5.906740004925704 28.441835645677692 22.863475905098106 42.78794844429849 14-15 11.091043428289959 32.61226500287332 17.679172481733847 38.61751908710286 16-17 16.09884245956818 30.839011575404317 17.145554552171415 35.916591412856086 18-19 12.203431573762417 27.908217716115264 16.45185124374025 43.436499466382074 20-21 9.440932599950742 29.197110253673753 17.736639027994418 43.62531811838109 22-23 19.202035957638948 29.184795993760776 14.49798867088088 37.1151793777194 24-25 13.88227567523192 35.674410967900826 13.516952631146866 36.926360725720386 26-27 10.508168459075609 36.626713734504555 18.746408340858714 34.11870946556112 28-29 14.296855758968885 30.785649782448075 22.736228552663984 32.181265905919055 30-31 14.415893604794352 30.09194647401691 24.722929151957967 30.76923076923077 32-33 9.84319842377473 36.75396108693867 22.149248830145307 31.253591659141282 34-35 9.670798784993021 34.85346030703555 20.991708398325258 34.48403250964617 36-37 11.275757326984648 29.779985222888104 23.692636072572036 35.25162137755521 38-39 11.485099745505295 24.480748707002707 28.515721205155568 35.51843034233643 40-41 8.468106066825385 26.898448403250963 26.36483047368853 38.26861505623512 42-43 4.301781462934078 25.400213447171826 30.25203185288564 40.045973237008454 44-45 6.341843855184304 27.296609473770626 31.914456941137836 34.44708972990723 46-47 6.50603398735736 31.750266808964785 26.38124948690584 35.36244971677202 48-49 6.616862326574173 34.71800344799277 20.314424103111403 38.350710122321644 50-51 6.7933667186602085 32.40292258435268 19.353911829899022 41.44979886708809 52-53 6.005254084229538 31.74205730235613 21.70183071997373 40.55085789344061 54-55 6.2925868155323865 31.553238650357113 19.49347344224612 42.660701091864375 56-57 10.807815450291438 31.04014448731631 17.900829160167476 40.25121090222478 58-59 9.403989820211805 30.132994007060177 18.610951481815942 41.85206469091208 60-61 6.518348247270339 27.366390279944174 19.657663574419175 46.45759789836631 62-63 9.075609555865691 29.87849930219194 17.527296609473773 43.5185945324686 64-65 9.551760939167556 31.118134800098513 13.89048518184057 45.43961907889336 66-67 6.978080617354896 39.26196535588211 11.550775798374517 42.209178228388474 68-69 9.42040883342911 38.26861505623512 10.881701009769312 41.42927510056646 70-71 6.354158115097283 42.935719563254246 10.09769312864297 40.612429193005504 72-73 9.26032345456038 39.910516377965685 12.34709793941384 38.482062228060094 74-75 9.252113947951727 38.46153846153847 15.643214842787948 36.643132747721864 76 10.721615630900583 33.90526229373615 18.881865199901483 36.49125687546179 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 2.0 11 2.0 12 1.0 13 1.0 14 1.5 15 2.5 16 4.0 17 5.0 18 6.5 19 8.5 20 8.5 21 8.0 22 11.0 23 21.5 24 29.0 25 29.0 26 29.5 27 39.0 28 52.0 29 56.0 30 78.0 31 109.5 32 142.0 33 165.0 34 177.5 35 214.5 36 262.0 37 285.0 38 333.5 39 408.5 40 474.0 41 563.0 42 613.0 43 650.0 44 700.0 45 734.0 46 755.0 47 787.5 48 817.5 49 767.5 50 720.0 51 698.0 52 639.0 53 577.0 54 552.0 55 512.5 56 451.0 57 377.5 58 326.0 59 301.5 60 243.0 61 211.5 62 214.0 63 176.0 64 123.5 65 98.0 66 74.0 67 61.0 68 56.5 69 47.5 70 38.5 71 34.0 72 27.5 73 17.5 74 12.5 75 11.0 76 9.0 77 6.5 78 4.0 79 2.0 80 4.5 81 4.5 82 2.0 83 2.0 84 3.0 85 3.0 86 3.0 87 4.0 88 2.0 89 1.5 90 2.5 91 3.0 92 4.0 93 2.5 94 0.5 95 1.5 96 3.0 97 2.5 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5746654626056974 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12181.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.44996305722026 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81013703153377 99.26114440522124 2 0.11556876341423147 0.22986618504227896 3 0.024764735017335313 0.07388555947787538 4 0.008254911672445105 0.03283802643461128 5 0.008254911672445105 0.0410475330432641 6 0.008254911672445105 0.04925703965191692 7 0.0 0.0 8 0.008254911672445105 0.06567605286922255 9 0.0 0.0 >10 0.01650982334489021 0.24628519825958461 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTG 16 0.1313521057384451 No Hit ATACACATCTGACGCTGCCGACGAACGAATGTGTGTAGATCTCGGTGGTC 14 0.11493309252113948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00820950660865282 0.0 2 0.0 0.0 0.0 0.00820950660865282 0.0 3 0.0 0.0 0.0 0.00820950660865282 0.0 4 0.0 0.0 0.0 0.00820950660865282 0.0 5 0.0 0.0 0.0 0.01641901321730564 0.0 6 0.0 0.0 0.0 0.01641901321730564 0.0 7 0.0 0.0 0.0 0.01641901321730564 0.0 8 0.0 0.0 0.0 0.01641901321730564 0.0 9 0.0 0.0 0.0 0.01641901321730564 0.0 10 0.0 0.0 0.0 0.02462851982595846 0.0 11 0.0 0.0 0.0 0.02462851982595846 0.0 12 0.0 0.0 0.0 0.02462851982595846 0.0 13 0.0 0.0 0.0 0.02462851982595846 0.0 14 0.0 0.0 0.0 0.02462851982595846 0.0 15 0.0 0.0 0.0 0.02462851982595846 0.0 16 0.0 0.0 0.0 0.02462851982595846 0.0 17 0.0 0.0 0.0 0.0410475330432641 0.0 18 0.0 0.0 0.0 0.0410475330432641 0.0 19 0.0 0.0 0.0 0.04925703965191692 0.0 20 0.0 0.0 0.0 0.04925703965191692 0.0 21 0.0 0.0 0.0 0.04925703965191692 0.0 22 0.0 0.0 0.0 0.04925703965191692 0.0 23 0.0 0.0 0.0 0.04925703965191692 0.0 24 0.0 0.0 0.0 0.04925703965191692 0.0 25 0.0 0.0 0.0 0.04925703965191692 0.0 26 0.0 0.0 0.0 0.04925703965191692 0.0 27 0.0 0.0 0.0 0.05746654626056974 0.0 28 0.0 0.0 0.0 0.05746654626056974 0.0 29 0.0 0.0 0.0 0.05746654626056974 0.0 30 0.0 0.0 0.0 0.06567605286922255 0.0 31 0.0 0.0 0.0 0.06567605286922255 0.0 32 0.0 0.0 0.0 0.07388555947787538 0.0 33 0.0 0.0 0.0 0.0820950660865282 0.0 34 0.0 0.0 0.0 0.09030457269518102 0.0 35 0.0 0.0 0.0 0.11493309252113948 0.0 36 0.0 0.0 0.0 0.1641901321730564 0.0 37 0.0 0.0 0.0 0.18881865199901485 0.0 38 0.0 0.0 0.0 0.22165667843362613 0.0 39 0.0 0.0 0.0 0.2627042114768902 0.0 40 0.0 0.0 0.0 0.27091371808554304 0.0 41 0.0 0.0 0.0 0.2955422379115015 0.0 42 0.0 0.0 0.0 0.3283802643461128 0.0 43 0.0 0.0 0.0 0.3612182907807241 0.0 44 0.0 0.0 0.0 0.4022658238239882 0.0 45 0.0 0.0 0.0 0.44331335686725226 0.0 46 0.0 0.0 0.0 0.46794187669321075 0.0 47 0.0 0.0 0.0 0.5007799031278221 0.0 48 0.0 0.0 0.0 0.5254084229537804 0.0 49 0.0 0.0 0.0 0.5500369427797389 0.0 50 0.0 0.0 0.0 0.5828749692143502 0.0 51 0.0 0.0 0.0 0.6239225022576144 0.0 52 0.0 0.0 0.0 0.6485510220835727 0.0 53 0.0 0.0 0.0 0.6567605286922256 0.0 54 0.0 0.0 0.0 0.6649700353008784 0.0 55 0.0 0.0 0.0 0.6895985551268369 0.0 56 0.0 0.0 0.0 0.7224365815614482 0.0 57 0.0 0.0 0.0 0.771693621213365 0.0 58 0.0 0.0 0.0 0.7881126344306707 0.0 59 0.0 0.0 0.0 0.7881126344306707 0.0 60 0.0 0.0 0.0 0.8045316476479764 0.0 61 0.0 0.0 0.0 0.8127411542566292 0.0 62 0.0 0.0 0.0 0.8455791806912405 0.0 63 0.0 0.0 0.0 0.8702077005171989 0.0 64 0.0 0.0 0.0 0.8784172071258517 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGACCC 20 7.3138806E-5 70.57262 1 CTTTACA 20 0.006571877 52.929462 1 TTACACA 40 0.0023280983 34.995884 3 TACCCCC 40 0.0023280983 34.995884 12 ACATCTG 80 0.0025214523 21.872427 8 >>END_MODULE