FastQCFastQC Report
Wed 25 May 2016
SRR1780476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT308325.309908874476644No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT250020.52376652163205TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC7706.321320088662671RNA PCR Primer, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG3873.1770790575486414No Hit
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC3833.1442410311140296TruSeq Adapter, Index 9 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT2061.6911583613824808RNA PCR Primer, Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT1931.5844347754699943No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1150.9440932599950743No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1050.8619981939085462No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT1030.8455791806912404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC980.8045316476479764No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT920.7552746079960595No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG890.730646088170101No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT840.6895985551268369No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG750.6157129956489615No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT750.6157129956489615No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG570.46794187669321075No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC570.46794187669321075No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC540.44331335686725226TruSeq Adapter, Index 9 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC500.41047533043264095TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCG410.3365897709547656No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCG410.3365897709547656No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG370.30375174452015435No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGATCTGATCTCGTATGCCGT340.27912322469419587No Hit
ATACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGTCTT340.27912322469419587Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGCGACTGGATCTGATCTCGTATGCCGT330.27091371808554304No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGT310.25449470486823744No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC310.25449470486823744TruSeq Adapter, Index 9 (95% over 21bp)
CTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT290.23807569165093176TruSeq Adapter, Index 9 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCT260.21344717182497333RNA PCR Primer, Index 9 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCG250.20523766521632048No Hit
TCTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT250.20523766521632048No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTC240.19702815860766767No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTC230.18881865199901487No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTCTGCCGTCTT220.18060914539036202No Hit
ATACACATCTCCGAGCCCACGAGAATGGATCTGATCTCGTATGCCGTCTT200.1641901321730564Illumina PCR Primer Index 9 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGCTCTCGTATGCCGTCTT180.14777111895575076No Hit
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTC180.14777111895575076No Hit
CTTATACACATATCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT180.14777111895575076No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTAGTATGCCGT180.14777111895575076No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAGCTCGTATGCCGT180.14777111895575076No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCCT180.14777111895575076TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCACACGAGACTGGATCTGATCTCGTATGCCGT170.13956161234709794No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTATT170.13956161234709794No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTC160.1313521057384451No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC160.1313521057384451No Hit
ATTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT150.12314259912979231No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCAGT150.12314259912979231No Hit
CTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTC150.12314259912979231No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGTC150.12314259912979231No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGC150.12314259912979231No Hit
ATACACATCTCCGAGCCCACGAGACTGGAACTGATCTCGTATGCCGTCTT140.11493309252113948Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAAATCGTATGCCGT140.11493309252113948No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGCTCTCGTATGCCGT140.11493309252113948No Hit
CTTATACACATCTCCGAGCCCACGAGAATGGATCTGATCTCGTATGCCGT130.10672358591248667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1000.070.000011
TTTACAC1000.070.000012
CTATACA850.065.882351
TTACACA1200.058.3333323
CTTATAC5550.056.1261251
TTATACA5750.054.1739122
AACAAAT200.006788457752.570
AAACACT200.006788457752.568
ACCACAA200.006788457752.570
AACCAAC200.006788457752.569
TCTTATA1050.049.9999961
TATACAC7050.044.6808553
ACTCGTA400.002326755635.038
AACTCGT400.002326755635.037
CTGAACT450.004144402731.11110934
GAACTCG450.004144402731.11110936
TCTGAAC450.004144402731.11110933
TGAACTC450.004144402731.11110935
ATACACA10350.030.0966194
GATATCG703.2180295E-530.036
Produced by FastQC (version 0.11.5)