##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12181 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73171332402923 34.0 34.0 34.0 31.0 34.0 2 33.249651095969135 34.0 34.0 34.0 32.0 34.0 3 33.45603809211067 34.0 34.0 34.0 34.0 34.0 4 33.54182743617108 34.0 34.0 34.0 34.0 34.0 5 33.56021673097447 34.0 34.0 34.0 34.0 34.0 6 37.425580822592565 38.0 38.0 38.0 37.0 38.0 7 36.9896560216731 38.0 37.0 38.0 36.0 38.0 8 36.97241605779492 38.0 37.0 38.0 36.0 38.0 9 36.68672522781381 38.0 37.0 38.0 34.0 38.0 10-11 36.3765700681389 38.0 37.0 38.0 34.0 38.0 12-13 35.99203677858961 38.0 36.5 38.0 33.0 38.0 14-15 36.01691158361382 38.0 36.0 38.0 32.5 38.0 16-17 35.91285608734915 38.0 36.0 38.0 32.0 38.0 18-19 36.063500533617926 38.0 36.5 38.0 32.5 38.0 20-21 35.92935719563254 38.0 36.0 38.0 31.5 38.0 22-23 35.9189721697726 38.0 36.5 38.0 32.5 38.0 24-25 34.52360233149987 37.0 34.0 38.0 28.0 38.0 26-27 34.68721779821033 37.0 34.0 38.0 27.0 38.0 28-29 34.6462113127001 37.0 34.5 38.0 26.5 38.0 30-31 34.01059026352516 37.0 34.0 38.0 25.0 38.0 32-33 34.29595271324193 37.0 34.0 38.0 26.0 38.0 34-35 33.49232411132091 37.0 33.0 38.0 19.0 38.0 36-37 33.08734915031607 37.0 34.0 38.0 21.0 38.0 38-39 30.645595599704457 34.0 27.0 37.0 11.0 38.0 40-41 32.986208028897465 36.5 32.5 37.0 23.5 38.0 42-43 34.97401691158362 37.0 34.5 38.0 29.0 38.0 44-45 35.3272309334209 37.0 36.0 38.0 31.0 38.0 46-47 35.3223873245218 37.0 36.0 38.0 31.0 38.0 48-49 34.829529595271325 37.5 35.0 38.0 28.0 38.0 50-51 34.75568508332649 37.0 34.0 38.0 28.0 38.0 52-53 34.467982924226256 37.0 34.0 38.0 26.5 38.0 54-55 34.93740251210902 37.0 35.5 38.0 27.0 38.0 56-57 34.71968639684755 37.0 35.0 38.0 27.0 38.0 58-59 34.4046465807405 37.0 34.0 38.0 26.0 38.0 60-61 34.41572941466218 37.0 34.0 38.0 25.5 38.0 62-63 35.11620556604548 37.0 36.0 38.0 29.0 38.0 64-65 34.94828010836549 37.0 35.0 38.0 29.0 38.0 66-67 32.70499138001806 37.0 31.0 38.0 21.5 38.0 68-69 28.02126262211641 32.5 21.5 37.0 11.0 38.0 70-71 23.82735407602003 24.5 15.0 33.5 10.5 37.0 72-73 19.872711600032837 21.0 11.0 25.0 10.5 34.0 74-75 19.2535095640752 19.0 11.0 25.0 11.0 34.0 76 19.552992365158854 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8657219165765611 1101 2 0.3686134756033326 1101 3 0.4230656856159385 1101 4 0.5782108956902121 1101 5 0.16237027610701205 1101 6 -0.8930723608448901 1101 7 0.2614417828271627 1101 8 0.5498101397539301 1101 9 0.7662659193391121 1101 10-11 -0.27398901942780185 1101 12-13 1.1413160491058747 1101 14-15 0.8844646580183948 1101 16-17 0.22942459812578875 1101 18-19 0.5748552542356293 1101 20-21 0.6357282181543837 1101 22-23 1.3195727210447004 1101 24-25 -2.399399103429616 1101 26-27 -0.0028102348002931876 1101 28-29 1.2875883968380393 1101 30-31 1.0714608408170037 1101 32-33 -0.1390552451735303 1101 34-35 1.9050030459090195 1101 36-37 -0.03631496938707812 1101 38-39 -0.11568688448883435 1101 40-41 2.3053570100779552 1101 42-43 0.6918654242450373 1101 44-45 1.089974979002804 1101 46-47 1.427756392983575 1101 48-49 -1.8712059233764435 1101 50-51 -2.775431712255937 1101 52-53 -3.3815561761764314 1101 54-55 -1.2728138619675988 1101 56-57 -3.1687702728793496 1101 58-59 -0.7347510674591078 1101 60-61 -2.418622412582124 1101 62-63 -2.1968081787681015 1101 64-65 -2.252904776978056 1101 66-67 -0.6209228606474042 1101 68-69 -0.316795175852814 1101 70-71 -0.5979567366708594 1101 72-73 -0.653945232465329 1101 74-75 -0.03476599282038606 1101 76 -1.515965457590923 1102 1 -1.2030280834234404 1102 2 0.17486347560333115 1102 3 0.3126490189492728 1102 4 0.5469608956902121 1102 5 0.2686202761070149 1102 6 0.44651097248843996 1102 7 0.11769178282716553 1102 8 0.2310601397539287 1102 9 1.0225159193391136 1102 10-11 1.2780943139055267 1102 12-13 0.8694410491058733 1102 14-15 0.3115479913517234 1102 16-17 0.4617162647924573 1102 18-19 0.37173025423562933 1102 20-21 -0.12885511517895054 1102 22-23 -0.20230227895530106 1102 24-25 -6.111899103429614 1102 26-27 -0.24968523480029603 1102 28-29 0.663630063504705 1102 30-31 3.144377507483668 1102 32-33 -0.22134691184019317 1102 34-35 0.3581280459090195 1102 36-37 -3.454023302720408 1102 38-39 0.0051464488444992185 1102 40-41 2.642857010077954 1102 42-43 2.0460320909117016 1102 44-45 1.566016645669471 1102 46-47 1.2662980596502464 1102 48-49 -3.486830923376438 1102 50-51 0.3401932877440643 1102 52-53 0.20177715715690425 1102 54-55 1.4063528046990683 1102 56-57 0.7999797271206504 1102 58-59 1.4214989325408922 1102 60-61 -5.959247412582123 1102 62-63 -2.2561831787681044 1102 64-65 -0.15082144364472327 1102 66-67 0.5457438060192601 1102 68-69 -2.7834618425194826 1102 70-71 -1.89795673667086 1102 72-73 -4.4174868991319975 1102 74-75 0.04440067384627966 1102 76 0.704867875742412 1103 1 0.8183534955239296 1103 2 0.4791397913928037 1103 3 -0.8699167705244122 1103 4 0.23610563253231476 1103 5 0.04658080242280249 1103 6 0.4086820251200223 1103 7 0.750915467037693 1103 8 0.7392838239644561 1103 9 0.682055393023326 1103 10-11 0.7830285244318418 1103 12-13 0.6334213122637635 1103 14-15 0.6651664124043535 1103 16-17 1.077670212160882 1103 18-19 1.4169605173935196 1103 20-21 0.33660541113683706 1103 22-23 0.4827306157815414 1103 24-25 -4.733609629745402 1103 26-27 -0.38702076111609074 1103 28-29 1.0147813792941847 1103 30-31 2.2776011916941883 1103 32-33 1.1100675618440192 1103 34-35 1.2076346248563894 1103 36-37 -1.5485956711414595 1103 38-39 -1.9849851301028707 1103 40-41 0.8895675363937485 1103 42-43 0.3322163014380166 1103 44-45 1.8908521719852587 1103 46-47 1.6207388491239314 1103 48-49 -2.563311186534335 1103 50-51 1.0745682877440643 1103 52-53 1.5701982097884795 1103 54-55 1.3043791204885409 1103 56-57 1.6207034113311778 1103 58-59 -2.129487909564368 1103 60-61 -5.55546451784528 1103 62-63 -1.4231239682417893 1103 64-65 0.6699022405658042 1103 66-67 -0.5989930360860001 1103 68-69 1.5060118416910449 1103 70-71 2.075727473855455 1103 72-73 1.1557038903416874 1103 74-75 -0.7680993261537203 1103 76 -1.5352637032049579 1104 1 0.5800076308622764 1104 2 0.14004204703190481 1104 3 0.3706847332349881 1104 4 -0.5646462471669338 1104 5 -0.2947725810358435 1104 6 0.1688324010598663 1104 7 0.24715606854145022 1104 8 -0.07876128881750333 1104 9 0.6519802050533983 1104 10-11 -0.8120842575230398 1104 12-13 0.34131604910587043 1104 14-15 1.1963694199231512 1104 16-17 -0.6610515923504039 1104 18-19 0.8105695399499098 1104 20-21 0.2809663133924758 1104 22-23 1.5052870067589907 1104 24-25 -0.3065419605724671 1104 26-27 0.8829040509139929 1104 28-29 2.004255063504708 1104 30-31 0.9166989360550986 1104 32-33 0.6371352310169485 1104 34-35 1.169288760194739 1104 36-37 2.616065982993881 1104 38-39 0.5319321631302145 1104 40-41 2.226785581506526 1104 42-43 1.958532090911703 1104 44-45 1.1494987885266141 1104 46-47 1.051565916793102 1104 48-49 -0.4212059233764407 1104 50-51 1.2959968591726394 1104 52-53 0.49463430001404873 1104 54-55 0.0652813761276434 1104 56-57 -0.14019884430792473 1104 58-59 -0.9918939246019605 1104 60-61 -0.34719384115354757 1104 62-63 1.3031918212318985 1104 64-65 1.3875714134981365 1104 66-67 0.21002952030497113 1104 68-69 5.252252443194802 1104 70-71 3.1734718347577093 1104 72-73 -1.0991833277034253 1104 74-75 -0.3109564690108648 1104 76 1.7173678757424113 1105 1 0.9323885832432239 1105 2 0.30707501406487125 1105 3 0.3999887625390173 1105 4 0.2448775623568764 1105 5 -0.4325015187647807 1105 6 0.15564558787305316 1105 7 0.2947751161605012 1105 8 -1.2296770397332537 1105 9 0.8278043808775735 1105 10-11 0.08498533954654874 1105 12-13 -2.07406856627874 1105 14-15 0.1601056836594168 1105 16-17 0.3883989571001507 1105 18-19 -0.25206782268745087 1105 20-21 -0.5014512690251038 1105 22-23 0.48880349027546544 1105 24-25 0.9890624350319186 1105 26-27 0.43821540622534627 1105 28-29 0.3555371147867561 1105 30-31 1.475306994663157 1105 32-33 1.6019703958521134 1105 34-35 -1.2424328515268783 1105 36-37 0.5688132357411249 1105 38-39 -0.22081508961704088 1105 40-41 0.14766470238564722 1105 42-43 0.06366029603990597 1105 44-45 1.3733083123361354 1105 46-47 1.5303204955476843 1105 48-49 -1.0801802823507956 1105 50-51 0.7091836723594511 1105 52-53 0.04152074690049545 1105 54-55 1.4656476764939441 1105 56-57 0.677383573274497 1105 58-59 0.008838676130629608 1105 60-61 1.0531724592127532 1105 62-63 -1.8839876659475934 1105 64-65 0.5150439409706635 1105 66-67 0.9239489342243843 1105 68-69 -1.0462823553399936 1105 70-71 1.64050480179068 1105 72-73 2.2960547675346668 1105 74-75 -0.5629711210255159 1105 76 -0.5082731498986135 1106 1 0.023297674152317427 1106 2 0.7080074149972688 1106 3 0.5654899280401793 1106 4 0.547907865387181 1106 5 0.3320672458039837 1106 6 0.3584427906702601 1106 7 -1.73552791414253 1106 8 0.4649616549054443 1106 9 -1.4822189291457377 1106 10-11 0.9835867381479488 1106 12-13 1.6019221097119356 1106 14-15 0.10416162771535653 1106 16-17 0.5597276284288171 1106 18-19 1.0369764663568475 1106 20-21 0.1978494302755962 1106 22-23 0.4165424180144015 1106 24-25 0.17787362384311223 1106 26-27 2.0244624924724306 1106 28-29 1.198194457444096 1106 30-31 0.3517638711200348 1106 32-33 0.4154902093719315 1106 34-35 0.42773031863629285 1106 36-37 1.9242910912189828 1106 38-39 1.575222206420257 1106 40-41 2.1068721615931025 1106 42-43 1.9645926969723106 1106 44-45 1.8278537668815957 1106 46-47 1.1398776051047932 1106 48-49 2.4757637735932576 1106 50-51 0.7942652574410332 1106 52-53 0.7548074601872088 1106 54-55 0.018095228941490404 1106 56-57 1.3160782119691348 1106 58-59 2.947067114359079 1106 60-61 2.2571351631754553 1106 62-63 2.3910706091106846 1106 64-65 -1.0059350800083635 1106 66-67 1.0381680484435023 1106 68-69 2.6786593696017285 1106 70-71 1.6474978087836867 1106 72-73 -0.5827331112532086 1106 74-75 0.1864461283917329 1106 76 0.1840345424090799 1107 1 0.983670634525275 1107 2 -0.43651472952487325 1107 3 0.5538349163851706 1107 4 0.552569870049183 1107 5 0.003395917132657189 1107 6 0.28385071607818446 1107 7 0.4999033212887056 1107 8 -0.3066001166563268 1107 9 0.10985566292885807 1107 10-11 0.649087903649118 1107 12-13 0.88746989525972 1107 14-15 1.1344646580183948 1107 16-17 1.478142546843742 1107 18-19 1.3504962798766513 1107 20-21 1.2421384745646407 1107 22-23 0.6554701569421297 1107 24-25 -0.5878606418911545 1107 26-27 -1.3694769014669674 1107 28-29 1.240152499402143 1107 30-31 2.51376853312469 1107 32-33 2.4609447548264782 1107 34-35 1.0267979177038882 1107 36-37 3.120095287023183 1107 38-39 1.8689285001265503 1107 40-41 0.7117672664882093 1107 42-43 1.4354551678347818 1107 44-45 0.7835647225925513 1107 46-47 1.5431410083681953 1107 48-49 1.67622997405946 1107 50-51 1.7604657236415022 1107 52-53 1.2081874135671598 1107 54-55 1.414365625211886 1107 56-57 1.8184092143001394 1107 58-59 1.9831976504896076 1107 60-61 2.3865057925460818 1107 62-63 1.9493456673857423 1107 64-65 1.4381208640475833 1107 66-67 -0.5504100401345866 1107 68-69 1.043461234403594 1107 70-71 -0.7569310956452178 1107 72-73 -1.4090734375935376 1107 74-75 -0.5629711210255159 1107 76 0.3378806962552332 1108 1 -0.5057066548520126 1108 2 0.3971849041747575 1108 3 0.1421133046635603 1108 4 0.5782108956902121 1108 5 0.2766559903927259 1108 6 0.025975258202727503 1108 7 0.04715606854145449 1108 8 0.5212387111824981 1108 9 0.48055163362482745 1108 10-11 1.24505859961981 1108 12-13 1.827030334820158 1108 14-15 0.8249408484945846 1108 16-17 0.35323412193531567 1108 18-19 0.05342668280705709 1108 20-21 1.2095377419639135 1108 22-23 -0.5518558503838733 1108 24-25 1.5648866108560995 1108 26-27 1.0686183366282762 1108 28-29 0.9471122063618438 1108 30-31 0.7024132217693833 1108 32-33 1.3085638024455193 1108 34-35 0.5121459030518736 1108 36-37 2.3732088401367335 1108 38-39 2.4176464488445006 1108 40-41 1.3839284386493844 1108 42-43 -0.8986107662311511 1108 44-45 0.7066416456694711 1108 46-47 0.8515659167930991 1108 48-49 1.7787940766235621 1108 50-51 1.053139716315492 1108 52-53 -0.37679427141452493 1108 54-55 -1.2347186238723609 1108 56-57 0.6312297271206475 1108 58-59 1.2938203611123171 1108 60-61 2.43852044456073 1108 62-63 1.8031918212318985 1108 64-65 1.844714270640992 1108 66-67 2.8957438060192544 1108 68-69 4.095109586051944 1108 70-71 -1.426528165242292 1108 72-73 -0.18489761341771072 1108 74-75 -0.15381361186800646 1108 76 1.2887964471709843 1109 1 -2.3658570307918616 1109 2 -0.04717599808088124 1109 3 0.41955691368611525 1109 4 0.10452668516389707 1109 5 0.12552817084385737 1109 6 -0.012370606458929956 1109 7 -0.19645295401493712 1109 8 0.02875750817498357 1109 9 0.26100276144437373 1109 10-11 -0.6511820018839529 1109 12-13 0.17289499647429807 1109 14-15 0.42832430714119596 1109 16-17 1.3671438963714024 1109 18-19 0.035381570025101894 1109 20-21 0.7576580427157893 1109 22-23 0.8774674578868087 1109 24-25 -1.1020306823769843 1109 26-27 0.07350555467338893 1109 28-29 -2.301008094390035 1109 30-31 0.2776011916941954 1109 32-33 1.6100675618440121 1109 34-35 -0.002891690933090274 1109 36-37 2.5698253814901193 1109 38-39 0.14659381726555054 1109 40-41 1.1132517469200636 1109 42-43 1.2269531435432768 1109 44-45 1.4697995404063136 1109 46-47 0.6470546385976164 1109 48-49 1.3972151292551445 1109 50-51 -0.05701065962436047 1109 52-53 0.2807245255779591 1109 54-55 0.09385280469906832 1109 56-57 -0.6029807991951373 1109 58-59 -0.037382646406477704 1109 60-61 0.44453548215471983 1109 62-63 1.0768760317582107 1109 64-65 1.0383232931973865 1109 66-67 0.03258591128241761 1109 68-69 -1.599251316203695 1109 70-71 -0.33216726298664767 1109 72-73 0.5109670482364237 1109 74-75 2.2845322527936496 1109 76 1.2278941915318846 1110 1 -1.3176114167567725 1110 2 0.10194680893666686 1110 3 0.6008434633937156 1110 4 0.16154422902354781 1110 5 0.3345924983292363 1110 6 -0.12640569417822434 1110 7 0.18366400504938696 1110 8 0.25536569530948583 1110 9 1.1440436971168921 1110 10-11 -0.9406556860944733 1110 12-13 0.11076049355031614 1110 14-15 0.8150202135739448 1110 16-17 0.7655357092369002 1110 18-19 -0.3140336346532564 1110 20-21 0.3051726625988209 1110 22-23 -1.0165383900664153 1110 24-25 -2.4660657700962787 1110 26-27 1.1444119874219254 1110 28-29 0.1375883968380336 1110 30-31 -1.706316936960775 1110 32-33 0.6970558659375854 1110 34-35 0.5300030459090195 1110 36-37 1.5164628083907061 1110 38-39 -2.1267979955999436 1110 40-41 -2.8085318788109355 1110 42-43 -1.7942456868660699 1110 44-45 0.6788638678916925 1110 46-47 -0.19724360701641785 1110 48-49 0.9871274099568907 1110 50-51 -0.53654282336705 1110 52-53 -0.8121117317319815 1110 54-55 0.857741693587954 1110 56-57 0.16456306045397895 1110 58-59 -3.331973289681329 1110 60-61 -1.3741779681376798 1110 62-63 -1.16069706765699 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-3.00418682826011 2116 66-67 -0.8773331170576668 2116 68-69 0.02423046517282401 2116 70-71 1.0058894171752932 2116 72-73 -0.043688822208917344 2116 74-75 -1.0565608646152604 2116 76 -0.29032443194989455 2117 1 0.29797998109268775 2117 2 0.6073231530226835 2117 3 0.3338183737879845 2117 4 -0.8411439430194676 2117 5 0.1688218890102391 2117 6 0.4112287144239204 2117 7 0.44531275056910147 2117 8 0.5627133655603771 2117 9 -0.5047018225963669 2117 10-11 -0.1503331054493131 2117 12-13 0.42841282329942487 2117 14-15 0.17747541070655615 2117 16-17 -1.400682928755927 2117 18-19 -1.8364350683450112 2117 20-21 -1.0734115667918545 2117 22-23 0.44537917265759575 2117 24-25 -1.210689426010255 2117 26-27 -3.122165073509972 2117 28-29 -0.6473578397211028 2117 30-31 -1.1086466860647164 2117 32-33 -2.7218509440982572 2117 34-35 0.2195191749412757 2117 36-37 -1.066422496268796 2117 38-39 0.38216257787675545 2117 40-41 -0.5123849254059181 2117 42-43 0.3585320909117016 2117 44-45 -0.7127131930402086 2117 46-47 -0.9700930693820098 2117 48-49 -2.205076891118374 2117 50-51 -1.288334938062384 2117 52-53 0.7098416732859363 2117 54-55 -0.502921388849316 2117 56-57 0.2021974690561379 2117 58-59 -4.105718809394592 2117 60-61 0.3910550067727172 2117 62-63 -0.28390495296165597 2117 64-65 -1.6760230565479475 2117 66-67 -0.6236110326904196 2117 68-69 1.2084736413514854 2117 70-71 1.24720455365172 2117 72-73 -0.7738377055836096 2117 74-75 -1.6229380358311403 2117 76 0.8399485209037039 2118 1 -1.3009447500901068 2118 2 -0.13138652439666743 2118 3 -3.4102676477173937 2118 4 -0.4551224376431193 2118 5 -0.03762972389298369 2118 6 -0.7597390275115572 2118 7 0.7281084494938312 2118 8 0.3831434730872587 2118 9 -2.0004007473275536 2118 10-11 -1.023989019427809 2118 12-13 -1.9253506175607953 2118 14-15 0.18446465801839196 2118 16-17 -5.587242068540874 2118 18-19 -2.091811412431035 2118 20-21 -0.7476051151789491 2118 22-23 -0.6137606122886297 2118 24-25 1.1506008965703884 2118 26-27 -3.369476901466964 2118 28-29 -1.1290782698286321 2118 30-31 -3.678539159183 2118 32-33 -1.1723885785068617 2118 34-35 -0.6783302874243091 2118 36-37 -5.186314969387077 2118 38-39 -2.4490202178221665 2118 40-41 -1.9946429899220455 2118 42-43 -0.8248012424216355 2118 44-45 -3.293358354330529 2118 46-47 -1.1722436070164193 2118 48-49 -1.8545392567097707 2118 50-51 -2.6920983789226014 2118 52-53 -3.414889509509763 2118 54-55 -1.4728138619676017 2118 56-57 0.047896393787318914 2118 58-59 -3.168084400792445 2118 60-61 -2.468622412582125 2118 62-63 -2.9801415121014365 2118 64-65 -1.4695714436447176 2118 66-67 -1.6542561939807392 2118 68-69 0.01653815748051457 2118 70-71 1.28537659666247 2118 72-73 2.3293881008680017 2118 74-75 3.298567340512946 2118 76 0.050701209075747045 2119 1 0.5734142242688662 2119 2 -1.616001909012052 2119 3 -0.3051394425891871 2119 4 -1.0756352581559412 2119 5 -0.06070664696990491 2119 6 -0.9982005659731001 2119 7 -0.3462505248651411 2119 8 -0.9732667833229911 2119 9 -0.24911869604549963 2119 10-11 -0.863732609171393 2119 12-13 0.06054681833664688 2119 14-15 -1.2822020086482766 2119 16-17 -0.6692933505921559 2119 18-19 0.8248552542356293 2119 20-21 -2.366835884409724 2119 22-23 -0.5496580481860676 2119 24-25 1.7390624350319257 2119 26-27 -0.26050254249260263 2119 28-29 -0.1765141672645214 2119 30-31 0.9112044305605878 2119 32-33 -0.3018757579940399 2119 34-35 0.09731073821671288 2119 36-37 -0.33503291810502134 2119 38-39 -0.3361997050016541 2119 40-41 -1.4484891437681995 2119 42-43 0.12776286014247518 2119 44-45 0.9758724149002447 2119 46-47 0.47903844426563325 2119 48-49 -2.336590538761051 2119 50-51 1.0168759800517577 2119 52-53 0.9325463879261306 2119 54-55 -0.00230104145477128 2119 56-57 -2.534154888263963 2119 58-59 -1.638597221305261 2119 60-61 -0.9340070279667358 2119 62-63 -1.1275774095373379 2119 64-65 -0.71572528979857 2119 66-67 0.4111284214038733 2119 68-69 0.6203843113266707 2119 70-71 1.10204326332914 2119 72-73 2.1678496393295426 2119 74-75 2.366516058461663 2119 76 -0.6749398165652813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 4.0 23 6.0 24 23.0 25 48.0 26 94.0 27 184.0 28 321.0 29 470.0 30 777.0 31 1086.0 32 1473.0 33 2073.0 34 2821.0 35 2286.0 36 478.0 37 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8455791806912404 30.94983991462113 11.944832115589852 56.25974878909778 2 0.41868483704129383 3.3166406698957394 86.65955176093917 9.6051227321238 3 0.5418274361710861 35.52253509564075 61.22650028733273 2.7091371808554308 4 0.3858468106066825 43.83876529020606 24.59568179952385 31.179706099663413 5 0.34479927756341844 53.07446022494048 42.86183400377637 3.7189064937197274 6 0.41868483704129383 45.776208849848125 9.161809375256546 44.64329693785403 7 0.9769312864296855 53.59165914128561 4.736885313192677 40.69452425909203 8 0.6157129956489615 44.815696576635744 30.79385928905673 23.774731138658566 9 0.6895985551268369 23.791150151875872 3.4069452425909206 72.11230605040637 10-11 0.6937033084311632 25.892783843690992 42.44725392003941 30.966258927838435 12-13 15.573434036614401 3.3412691897216975 32.809293161480994 48.276003612182905 14-15 23.91839750430999 16.940316886955095 7.212051555701502 51.92923405303341 16-17 31.93498070765947 26.685001231425993 0.8414744273869141 40.538543633527624 18-19 25.765536491256874 22.354486495361627 0.5336179295624333 51.34635908381906 20-21 17.736639027994418 9.954026762991544 0.7675888679090387 71.541745341105 22-23 24.029225843526806 40.12396354979066 0.6075034890403087 35.239307117642234 24-25 32.074542320006564 44.37648797307282 1.2929972908628191 22.255972416057794 26-27 40.69452425909203 32.82981692800263 16.767917248173383 9.70774156473196 28-29 27.45669485263936 21.927592151711682 26.47976356620967 24.13594942943929 30-31 49.174944585830396 9.658484525080043 23.146703883096624 18.01986700599294 32-33 24.094901896396024 23.75831212544126 42.47188243986537 9.674903538297349 34-35 9.621541745341105 19.74386339381003 46.33856005254084 24.296034808308022 36-37 23.787045398571546 27.5880469583778 29.862080288974635 18.76282735407602 38-39 17.851572120515556 10.023807569165093 30.313603152450536 41.81101715786881 40-41 9.39988506690748 16.570889089565714 41.556522453000575 32.47270339052623 42-43 38.15368196371398 9.297266234299318 44.897791642722275 7.651260159264428 44-45 11.390690419505788 23.618750513094163 33.20745423200066 31.783104835399396 46-47 39.64781216648879 2.9390033658977095 22.399638781709218 35.013545685904276 48-49 28.478778425416635 1.231425991297923 24.2303587554388 46.059436827846646 50-51 7.1545850094409325 1.1288071586897628 48.20622280600936 43.51038502585995 52-53 15.364091618093752 1.1493309252113948 67.592972662343 15.893604794351859 54-55 9.133076102126262 1.2355307446022494 49.794762334783684 39.83663081848781 56-57 38.07569165093178 1.4777111895575077 31.639438469747965 28.807158689762748 58-59 11.119776701420244 32.00065676052869 49.236515885395285 7.643050652655775 60-61 23.27805598883507 65.45029143748461 9.321894754125278 1.949757819555045 62-63 2.610623101551597 95.02914374846073 0.8989409736474838 1.461292176340202 64-65 0.5336179295624333 96.843444708973 0.5951892291273295 2.0277481323372464 66-67 0.3694277973893769 94.47089729907232 0.890731467038831 4.268943436499466 68-69 1.0672358591248665 85.05048846564321 2.783022740333306 11.099252934898612 70-71 2.2247762909449142 75.26065183482473 5.668664313274772 16.845907560955588 72-73 3.3576882029390034 61.994089155241774 9.445037353255069 25.203185288564157 74-75 4.507019128150398 59.211066414908466 9.395780313603153 26.886134143337987 76 3.5218783351120595 61.776537230112474 8.521467859781627 26.180116574993846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 1.0 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 2.0 23 2.0 24 0.0 25 0.0 26 1.0 27 1.0 28 0.5 29 1.0 30 1.0 31 3.5 32 4.0 33 2.0 34 2.5 35 5.5 36 16.0 37 24.0 38 49.5 39 251.0 40 1155.5 41 2418.5 42 2953.0 43 2911.5 44 2383.5 45 1472.5 46 1048.0 47 776.5 48 368.5 49 168.5 50 105.0 51 76.0 52 35.5 53 22.0 54 20.0 55 16.5 56 10.0 57 5.5 58 4.0 59 3.0 60 3.0 61 4.0 62 4.0 63 2.5 64 1.5 65 1.5 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 12181.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.049585419916262 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25575447570333 12.880715868976274 2 8.286445012787723 2.6598801412035136 3 3.0179028132992327 1.453082669731549 4 1.329923273657289 0.8537886872998933 5 0.8695652173913043 0.6978080617354897 6 0.7672634271099744 0.7388555947787538 7 0.6138107416879796 0.6895985551268369 8 0.5626598465473146 0.7224365815614481 9 0.35805626598465473 0.5171989163451277 >10 2.9667519181585678 8.79238157786717 >50 0.46035805626598464 5.59067400049257 >100 0.35805626598465473 12.248583860110006 >500 0.051150895140664954 6.321320088662671 >1k 0.10230179028132991 45.83367539610869 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 3083 25.309908874476644 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT 2500 20.52376652163205 TruSeq Adapter, Index 9 (95% over 23bp) CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 770 6.321320088662671 RNA PCR Primer, Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 387 3.1770790575486414 No Hit CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 383 3.1442410311140296 TruSeq Adapter, Index 9 (95% over 21bp) TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT 206 1.6911583613824808 RNA PCR Primer, Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT 193 1.5844347754699943 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT 115 0.9440932599950743 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 105 0.8619981939085462 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT 103 0.8455791806912404 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 98 0.8045316476479764 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT 92 0.7552746079960595 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 89 0.730646088170101 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT 84 0.6895985551268369 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 75 0.6157129956489615 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT 75 0.6157129956489615 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 57 0.46794187669321075 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 57 0.46794187669321075 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 54 0.44331335686725226 TruSeq Adapter, Index 9 (95% over 21bp) TTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 50 0.41047533043264095 TruSeq Adapter, Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCG 41 0.3365897709547656 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCG 41 0.3365897709547656 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 37 0.30375174452015435 No Hit CTTATACACATCTCCGAGCCCACGAGCCTGGATCTGATCTCGTATGCCGT 34 0.27912322469419587 No Hit ATACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGTCTT 34 0.27912322469419587 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGCGACTGGATCTGATCTCGTATGCCGT 33 0.27091371808554304 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGT 31 0.25449470486823744 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 31 0.25449470486823744 TruSeq Adapter, Index 9 (95% over 21bp) CTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT 29 0.23807569165093176 TruSeq Adapter, Index 9 (95% over 22bp) ACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCT 26 0.21344717182497333 RNA PCR Primer, Index 9 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCG 25 0.20523766521632048 No Hit TCTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 25 0.20523766521632048 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTC 24 0.19702815860766767 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTC 23 0.18881865199901487 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTCTGCCGTCTT 22 0.18060914539036202 No Hit ATACACATCTCCGAGCCCACGAGAATGGATCTGATCTCGTATGCCGTCTT 20 0.1641901321730564 Illumina PCR Primer Index 9 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACTGGATCTGCTCTCGTATGCCGTCTT 18 0.14777111895575076 No Hit CTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTC 18 0.14777111895575076 No Hit CTTATACACATATCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 18 0.14777111895575076 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTAGTATGCCGT 18 0.14777111895575076 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAGCTCGTATGCCGT 18 0.14777111895575076 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCCT 18 0.14777111895575076 TruSeq Adapter, Index 9 (95% over 21bp) CTTATACACATCTCCGAGCACACGAGACTGGATCTGATCTCGTATGCCGT 17 0.13956161234709794 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTATT 17 0.13956161234709794 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTC 16 0.1313521057384451 No Hit CTTATATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 16 0.1313521057384451 No Hit ATTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 15 0.12314259912979231 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCAGT 15 0.12314259912979231 No Hit CTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTC 15 0.12314259912979231 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGCTCTGATCTCGTATGCCGTC 15 0.12314259912979231 No Hit TCTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGC 15 0.12314259912979231 No Hit ATACACATCTCCGAGCCCACGAGACTGGAACTGATCTCGTATGCCGTCTT 14 0.11493309252113948 Illumina Paired End PCR Primer 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAAATCGTATGCCGT 14 0.11493309252113948 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGCTCTCGTATGCCGT 14 0.11493309252113948 No Hit CTTATACACATCTCCGAGCCCACGAGAATGGATCTGATCTCGTATGCCGT 13 0.10672358591248667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01641901321730564 0.0 2 0.0 0.0 0.0 0.01641901321730564 0.0 3 0.0 0.0 0.0 0.01641901321730564 0.0 4 0.0 0.0 0.0 0.01641901321730564 0.0 5 0.0 0.0 0.0 0.03283802643461128 0.0 6 0.0 0.0 0.0 0.03283802643461128 0.0 7 0.0 0.0 0.0 0.03283802643461128 0.0 8 0.0 0.0 0.0 0.03283802643461128 0.0 9 0.0 0.0 0.0 0.03283802643461128 0.0 10 0.0 0.0 0.0 0.03283802643461128 0.0 11 0.0 0.0 0.0 0.03283802643461128 0.0 12 0.0 0.0 0.0 0.03283802643461128 0.0 13 0.0 0.0 0.0 0.03283802643461128 0.0 14 0.0 0.0 0.0 0.03283802643461128 0.0 15 0.0 0.0 0.0 0.03283802643461128 0.0 16 0.0 0.0 0.0 0.03283802643461128 0.0 17 0.0 0.0 0.0 0.04925703965191692 0.0 18 0.0 0.0 0.0 0.05746654626056974 0.0 19 0.0 0.0 0.0 0.06567605286922255 0.0 20 0.0 0.0 0.0 0.06567605286922255 0.0 21 0.0 0.0 0.0 0.06567605286922255 0.0 22 0.0 0.0 0.0 0.06567605286922255 0.0 23 0.0 0.0 0.0 0.06567605286922255 0.0 24 0.0 0.0 0.0 0.06567605286922255 0.0 25 0.0 0.0 0.0 0.06567605286922255 0.0 26 0.0 0.0 0.0 0.06567605286922255 0.0 27 0.0 0.0 0.0 0.06567605286922255 0.0 28 0.0 0.0 0.0 0.06567605286922255 0.0 29 0.0 0.0 0.0 0.06567605286922255 0.0 30 0.0 0.0 0.0 0.07388555947787538 0.0 31 0.0 0.0 0.0 0.07388555947787538 0.0 32 0.0 0.0 0.0 0.07388555947787538 0.0 33 0.0 0.0 0.0 0.07388555947787538 0.0 34 0.0 0.0 0.0 0.07388555947787538 0.0 35 0.0 0.0 0.0 0.0820950660865282 0.0 36 0.0 0.0 0.0 0.09030457269518102 0.0 37 0.0 0.0 0.0 0.11493309252113948 0.0 38 0.0 0.0 0.0 0.1231425991297923 0.0 39 0.0 0.0 0.0 0.1313521057384451 0.0 40 0.0 0.0 0.0 0.14777111895575076 0.0 41 0.0 0.0 0.0 0.1641901321730564 0.0 42 0.0 0.0 0.0 0.2052376652163205 0.0 43 0.0 0.0 0.0 0.21344717182497333 0.0 44 0.0 0.0 0.0 0.2380756916509318 0.0 45 0.0 0.0 0.0 0.2462851982595846 0.0 46 0.0 0.0 0.0 0.2462851982595846 0.0 47 0.0 0.0 0.0 0.27091371808554304 0.0 48 0.0 0.0 0.0 0.27091371808554304 0.0 49 0.0 0.0 0.0 0.27912322469419587 0.0 50 0.0 0.0 0.0 0.2955422379115015 0.0 51 0.0 0.0 0.0 0.3283802643461128 0.0 52 0.0 0.0 0.0 0.3365897709547656 0.0 53 0.0 0.0 0.0 0.3365897709547656 0.0 54 0.0 0.0 0.0 0.3365897709547656 0.0 55 0.0 0.0 0.0 0.34479927756341844 0.0 56 0.0 0.0 0.0 0.3776373039980297 0.0 57 0.0 0.0 0.0 0.410475330432641 0.0 58 0.0 0.0 0.0 0.410475330432641 0.0 59 0.0 0.0 0.0 0.41868483704129383 0.0 60 0.0 0.0 0.0 0.42689434364994666 0.0 61 0.0 0.0 0.0 0.43510385025859943 0.0 62 0.0 0.0 0.0 0.4515228634759051 0.0 63 0.0 0.0 0.0 0.4597323700845579 0.0 64 0.0 0.0 0.0 0.4597323700845579 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 100 0.0 70.00001 1 TTTACAC 100 0.0 70.00001 2 CTATACA 85 0.0 65.88235 1 TTACACA 120 0.0 58.333332 3 CTTATAC 555 0.0 56.126125 1 TTATACA 575 0.0 54.173912 2 AACAAAT 20 0.0067884577 52.5 70 AAACACT 20 0.0067884577 52.5 68 ACCACAA 20 0.0067884577 52.5 70 AACCAAC 20 0.0067884577 52.5 69 TCTTATA 105 0.0 49.999996 1 TATACAC 705 0.0 44.680855 3 ACTCGTA 40 0.0023267556 35.0 38 AACTCGT 40 0.0023267556 35.0 37 CTGAACT 45 0.0041444027 31.111109 34 GAACTCG 45 0.0041444027 31.111109 36 TCTGAAC 45 0.0041444027 31.111109 33 TGAACTC 45 0.0041444027 31.111109 35 ATACACA 1035 0.0 30.096619 4 GATATCG 70 3.2180295E-5 30.0 36 >>END_MODULE