FastQCFastQC Report
Wed 25 May 2016
SRR1780475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113606
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1470.12939457423023432No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC1330.11707128144640248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT301.2799718E-436.7332314
ATGATCG250.002343275135.20176330
TGATCGT250.00234834835.1862631
CGTGTAT505.9084414E-835.17077635
CTTTACA1800.034.2692261
CTTATCA401.7887574E-533.0453261
TATCTCG401.8159595E-532.97260341
TTTACAC2200.030.974262
CTTATAC8050.029.5560681
AAAGGTT300.005685096629.3736231
TATCACA454.0605002E-529.3089793
ATCACAT454.0605002E-529.3089794
TCAGGTC300.005746689629.30897938
TCTACAC300.005746689629.3089793
CTCTGTG454.0605002E-529.30897944
TTTCTAC300.005746689629.30897935
TTCTCCA300.005746689629.3089792
TTATACA7750.028.9307982
TGTATAT554.941143E-627.97675137
CTATACA804.1673047E-927.5377711
Produced by FastQC (version 0.11.5)