SRR1780475_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780475_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113606
Sequences flagged as poor quality	0
Sequence length	50
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	44164	38.87470732179638	No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT	29398	25.877154375649177	No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC	8267	7.276904388852702	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	6766	5.955671355386159	No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC	4743	4.174955548122458	No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT	2426	2.135450592398289	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	1703	1.4990405436332588	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	1505	1.3247539742619228	No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	1124	0.9893843635019278	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	820	0.7217928630530078	No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC	769	0.6769008679119061	No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	700	0.6161646391915919	No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC	589	0.5184585321197823	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	588	0.5175782969209373	No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	446	0.39258489868492863	No Hit
ACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTTCT	421	0.37057901871380033	Illumina PCR Primer Index 6 (95% over 22bp)
CTTATCACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC	339	0.29839973240849954	No Hit
CTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT	300	0.2640705596535394	No Hit
TCTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	289	0.25438797246624295	No Hit
ATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTTCTGCTT	265	0.23326232769395983	Illumina PCR Primer Index 6 (96% over 26bp)
CTTATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC	228	0.20069362533668994	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	193	0.16988539337711037	No Hit
TCTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT	188	0.1654842173828847	No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGC	143	0.12587363343485378	No Hit
CTCTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG	142	0.12499339823600866	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTTAT	160	0.0	43.996918	1
TCTTTAC	20	7.8292226E-4	43.996918	1
CCGTCTT	3150	0.0	43.01921	44
CTTTACA	870	0.0	42.479782	1
TTTACAC	880	0.0	41.997063	2
TCTTAAT	100	0.0	41.797073	1
TATAACA	80	0.0	41.247112	3
TTATAAC	80	0.0	41.247112	2
ATAACAC	80	0.0	41.247112	4
CTTAACA	75	0.0	41.06379	1
GTCTTCT	55	7.6397555E-11	39.9972	44
CTATACA	540	0.0	39.92313	1
CTTATCA	45	2.3204848E-8	39.10837	1
CTTATAA	90	0.0	39.10837	1
GCCGTCT	3480	0.0	38.9398	43
CTTACAC	40	4.083231E-7	38.497303	1
TTAACAC	80	0.0	38.497303	2
TTATTAC	70	0.0	37.711643	2
ATTACAC	70	0.0	37.711643	4
TATTACA	70	0.0	37.711643	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content