FastQCFastQC Report
Wed 25 May 2016
SRR1780474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42485
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATG150.002211460869.98942
TTACCCC252.3665809E-455.9915162
TTTGTGA200.006910374452.49204633
TCTGTAT200.006910374452.49204653
AATTCTT200.006910374452.49204639
TGAACCG800.002627785621.87168770
CCCTCCT850.003735111320.5851179
TTGAACC850.003735111320.58511769
AATTGAA1107.323683E-419.08801867
ACCCTGT950.007105847418.41826254
GACGAAT1806.904191E-515.55320170
CGACGAA2407.8992685E-411.664969
CCGACGA2850.0033103539.82307368
GCCGACG3000.0050571149.3319267
TCTGACG5200.0078628936.7297559
Produced by FastQC (version 0.11.5)