##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42485 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.193550664940567 32.0 21.0 34.0 12.0 34.0 2 28.575826762386725 34.0 23.0 34.0 11.0 34.0 3 28.67981640579028 34.0 23.0 34.0 11.0 34.0 4 29.298387666235143 34.0 27.0 34.0 11.0 34.0 5 29.158973755443096 34.0 27.0 34.0 11.0 34.0 6 31.239425679651642 37.0 27.0 38.0 11.0 38.0 7 30.551771213369424 36.0 26.0 38.0 11.0 38.0 8 29.50245969165588 34.0 23.0 37.0 11.0 38.0 9 30.390867364952335 36.0 26.0 38.0 12.0 38.0 10-11 30.798740732023067 36.0 26.0 38.0 11.0 38.0 12-13 31.18461810050606 37.0 26.5 38.0 11.0 38.0 14-15 30.7801930092974 36.0 26.0 38.0 11.0 38.0 16-17 30.554607508532424 36.0 26.0 38.0 11.0 38.0 18-19 30.60590796751795 36.5 25.0 38.0 11.0 38.0 20-21 30.754642815111215 36.0 26.0 38.0 11.0 38.0 22-23 30.60727315523126 36.5 26.0 38.0 11.0 38.0 24-25 31.090314228551254 37.0 26.5 38.0 11.0 38.0 26-27 31.077050723784865 37.0 26.0 38.0 11.0 38.0 28-29 31.138390020007062 37.0 26.5 38.0 11.0 38.0 30-31 31.005437213134048 37.0 26.0 38.0 11.0 38.0 32-33 30.861562904554546 37.0 26.0 38.0 11.0 38.0 34-35 30.71216900082382 36.5 26.0 38.0 11.0 38.0 36-37 30.620195363069318 36.5 26.0 38.0 11.0 38.0 38-39 31.11994821701777 37.0 26.5 38.0 11.0 38.0 40-41 30.67409674002589 36.5 26.0 38.0 11.0 38.0 42-43 31.023667176650584 37.0 26.5 38.0 11.0 38.0 44-45 30.090643756619983 36.0 24.5 38.0 11.0 38.0 46-47 30.708826644698128 36.0 26.0 38.0 11.0 38.0 48-49 30.556490526068025 36.0 26.0 38.0 11.0 38.0 50-51 30.526350476638815 36.0 25.5 38.0 11.0 38.0 52-53 30.47081322819819 36.0 25.5 38.0 11.0 38.0 54-55 30.166670589619866 36.0 24.0 38.0 11.0 38.0 56-57 30.228186418736023 36.0 24.5 38.0 11.0 38.0 58-59 30.03706013887254 35.5 24.5 38.0 11.0 38.0 60-61 30.146875367776865 36.0 24.5 38.0 11.0 38.0 62-63 30.157761562904554 36.0 24.5 38.0 11.0 38.0 64-65 30.207426150406025 36.0 24.0 38.0 11.0 38.0 66-67 30.236318700717902 36.0 24.5 38.0 11.0 38.0 68-69 29.74375661998352 35.5 23.5 38.0 11.0 38.0 70-71 29.59486877721549 35.0 23.5 37.5 11.0 38.0 72-73 29.65924443921384 35.0 24.0 38.0 11.0 38.0 74-75 29.39509238554784 34.0 23.5 37.0 11.0 38.0 76 28.70914440390726 33.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.2433558544523073 1101 2 -2.9436673505742803 1101 3 -1.510579286198098 1101 4 -2.9517920043722974 1101 5 -0.9787150930690558 1101 6 -2.222111794053834 1101 7 -1.9642285268005217 1101 8 -1.464750680990626 1101 9 -1.9317625834399017 1101 10-11 -1.3563669673190404 1101 12-13 -2.8894262436721974 1101 14-15 -1.8485347290127692 1101 16-17 -4.15969811502201 1101 18-19 -1.696228456393019 1101 20-21 -2.9243838026660747 1101 22-23 -3.1939639107499964 1101 24-25 -2.8046842461025605 1101 26-27 -3.4550077238380013 1101 28-29 -2.035485556532418 1101 30-31 -3.661884952200424 1101 32-33 -1.8094812126128694 1101 34-35 -1.8611499418339221 1101 36-37 -0.9068668320133035 1101 38-39 -2.314732057732627 1101 40-41 -1.7152099513773678 1101 42-43 -2.4369285117493504 1101 44-45 -1.4059980018260134 1101 46-47 -1.0228926762495014 1101 48-49 -2.924247696829376 1101 50-51 -2.667664478892764 1101 52-53 -2.0049931645770123 1101 54-55 -2.0263050757259293 1101 56-57 -1.8618360209138771 1101 58-59 -1.7775031713523255 1101 60-61 -1.4493600792778665 1101 62-63 -3.085472452644904 1101 64-65 -2.2840885887323665 1101 66-67 -3.752397466077401 1101 68-69 -1.609440137874607 1101 70-71 -1.6950048359253813 1101 72-73 -2.7088327291012284 1101 74-75 -4.081157788321189 1101 76 -3.1966356819319586 1102 1 0.8734137836765541 1102 2 0.5412399751097468 1102 3 0.11969432368716326 1102 4 0.614227413103432 1102 5 -0.14102753790577083 1102 6 -0.2078134710176478 1102 7 0.8148535561650547 1102 8 2.6945608812335564 1102 9 1.57662223562453 1102 10-11 -0.2091471967982983 1102 12-13 -3.726804884448896 1102 14-15 -0.8520210485185054 1102 16-17 -1.2409867293203298 1102 18-19 0.246048683942373 1102 20-21 -0.2317977479440998 1102 22-23 -0.7449789151630597 1102 24-25 0.2331357009406858 1102 26-27 -4.506772948904196 1102 28-29 -1.0385305697716056 1102 30-31 0.9101362305003704 1102 32-33 -6.435121106699363 1102 34-35 -0.8889963672531636 1102 36-37 0.38082072314997717 1102 38-39 0.3775450826027651 1102 40-41 1.2896885481813314 1102 42-43 0.12088260916856441 1102 44-45 -5.916677613476502 1102 46-47 0.24127325490671936 1102 48-49 0.47676730758368535 1102 50-51 -5.554248768389673 1102 52-53 -1.9082588309494746 1102 54-55 1.653747881026053 1102 56-57 -0.9463020403313536 1102 58-59 0.3433706150554414 1102 60-61 0.5770741660884191 1102 62-63 -3.978764597393358 1102 64-65 -0.8772483416008541 1102 66-67 -0.22530126131129435 1102 68-69 -6.975900861616886 1102 70-71 -0.3029483487232625 1102 72-73 0.029428524208565676 1102 74-75 -5.256135723007862 1102 76 -2.3929287092929457 1103 1 -0.7052793981416272 1103 2 -0.027936161253890646 1103 3 0.27764886914170717 1103 4 0.19391491310343056 1103 5 0.25414291663968314 1103 6 -1.4338077891994665 1103 7 1.0284899198014195 1103 8 0.3422881539608298 1103 9 -0.19397435528456342 1103 10-11 0.31869371229261034 1103 12-13 -2.187742384448896 1103 14-15 -0.6270920712457766 1103 16-17 -0.457179911138514 1103 18-19 0.2175685703060104 1103 20-21 -0.9588574070350084 1103 22-23 -0.550873801526695 1103 24-25 0.26786581457704983 1103 26-27 -1.360537153449652 1103 28-29 0.07347227113748644 1103 30-31 0.03293452595491431 1103 32-33 -3.95458133397209 1103 34-35 -1.9594509127077089 1103 36-37 -1.0685400723045682 1103 38-39 -0.2993725310335975 1103 40-41 0.1502709345449631 1103 42-43 0.3822462455322011 1103 44-45 -6.138552613476499 1103 46-47 -1.4141244723660087 1103 48-49 -0.6689003060526773 1103 50-51 -0.7520470638442198 1103 52-53 -0.8007304218585638 1103 54-55 -0.08808450533758005 1103 56-57 0.188072959668645 1103 58-59 0.12696436505544284 1103 60-61 -0.2700139020933996 1103 62-63 -1.137429370120632 1103 64-65 -0.05494720523721952 1103 66-67 -0.7417785340385663 1103 68-69 -2.888755975253247 1103 70-71 -0.4280903941778078 1103 72-73 -0.4675174985187063 1103 74-75 -2.414516404826042 1103 76 -1.4352582547474917 1104 1 -1.9301435285764086 1104 2 0.1744415561374133 1104 3 0.5129749560982297 1104 4 -0.03359867385309201 1104 5 0.1787353079440308 1104 6 -0.739038767460336 1104 7 1.0958812241492453 1104 8 0.5455490235260463 1104 9 -0.42733033354543437 1104 10-11 0.37385675577086985 1104 12-13 -0.9637614061880271 1104 14-15 -0.4877917995066454 1104 16-17 -0.3228049111385154 1104 18-19 0.8258905811755746 1104 20-21 0.9025896038345564 1104 22-23 0.19691831803852367 1104 24-25 0.446772064577047 1104 26-27 0.7234642052459996 1104 28-29 0.07629156461574738 1104 30-31 -0.35310487621899966 1104 32-33 0.6745626877670396 1104 34-35 -0.2192335214033605 1104 36-37 0.04623574291282395 1104 38-39 0.6291261102707466 1104 40-41 0.715182619327571 1104 42-43 0.7702897237930699 1104 44-45 -2.6601558743460636 1104 46-47 0.8531309624165999 1104 48-49 0.9544352917734074 1104 50-51 1.1611662513731709 1104 52-53 0.43126686075013154 1104 54-55 -0.22609401620714564 1104 56-57 0.46239361184255756 1104 58-59 1.5977184411423977 1104 60-61 0.7219358261674707 1104 62-63 0.6745950864011085 1104 64-65 0.8699848599801712 1104 66-67 0.8976236398744746 1104 68-69 -2.7960589643836826 1104 70-71 0.5081188449526266 1104 72-73 1.6197447297421625 1104 74-75 -0.47056260047821397 1104 76 1.0007471800351162 1105 1 0.9221741142550677 1105 2 0.2205788180849524 1105 3 -1.0927023705277144 1105 4 -0.13866514888004033 1105 5 -0.526977951128913 1105 6 -0.7400448759763272 1105 7 0.09667173798323603 1105 8 0.36811460024182097 1105 9 -1.0018901610696886 1105 10-11 -0.32980835382309337 1105 12-13 -1.092507363787739 1105 14-15 -0.5284673295102387 1105 16-17 0.19868269216727086 1105 18-19 0.3353048822894813 1105 20-21 0.05002043387408506 1105 22-23 -0.6325822209481835 1105 24-25 -0.32017008418328174 1105 26-27 -1.055946502623204 1105 28-29 -1.033158668945159 1105 30-31 -0.4799464141277312 1105 32-33 -0.31983185050101426 1105 34-35 -0.4067649622944849 1105 36-37 -0.046038780982254224 1105 38-39 -1.1220416942567368 1105 40-41 -1.4487412038847864 1105 42-43 -0.3708529280215167 1105 44-45 0.3081157749532544 1105 46-47 -0.17277633187014274 1105 48-49 -0.12612525439978128 1105 50-51 -0.7815214956623997 1105 52-53 -1.868589409461869 1105 54-55 -1.6313760859160915 1105 56-57 -0.8727483213230904 1105 58-59 -0.4140674014734813 1105 60-61 -1.5811902967214984 1105 62-63 0.11792961748267672 1105 64-65 -0.25452106887358283 1105 66-67 -1.3319128315592295 1105 68-69 0.20095864251534934 1105 70-71 0.7185392545825238 1105 72-73 -0.017265690667468192 1105 74-75 -1.3739043180491812 1105 76 -1.502019618383855 1106 1 0.4872477757714151 1106 2 1.1657459039635008 1106 3 0.8955836517504032 1106 4 0.5403143696251682 1106 5 1.2135179166396846 1106 6 0.6609612325396661 1106 7 1.7045768763231557 1106 8 -0.21097271560438813 1106 9 2.337887057758916 1106 10-11 1.2608132775100067 1106 12-13 1.601455985116317 1106 14-15 0.9382951570150908 1106 16-17 2.2989342192962674 1106 18-19 1.0215427550886176 1106 20-21 2.1721548212258632 1106 22-23 2.2577878832559115 1106 24-25 1.5163372819683545 1106 26-27 0.9756381182894778 1106 28-29 0.015421999398359532 1106 30-31 1.3121125150853459 1106 32-33 2.5310844268974755 1106 34-35 2.0938099568575126 1106 36-37 2.1288444385650003 1106 38-39 1.868256545053356 1106 40-41 1.0064869671536556 1106 42-43 2.3746375498800276 1106 44-45 2.918104995219153 1106 46-47 2.0444353102426867 1106 48-49 1.9674787700342762 1106 50-51 2.3916010339818676 1106 52-53 1.283440773793611 1106 54-55 1.086949462053724 1106 56-57 1.9189153509729948 1106 58-59 0.7542401802728342 1106 60-61 2.834979304428341 1106 62-63 2.200682042922846 1106 64-65 1.8960718165019088 1106 66-67 1.341101900744043 1106 68-69 2.056114948659797 1106 70-71 1.0646405840830617 1106 72-73 0.8327882080030342 1106 74-75 0.33378522560873947 1106 76 1.1572689191655527 1107 1 -0.41627841388966047 1107 2 0.2859787009508352 1107 3 0.35884965654328127 1107 4 0.31737701940264174 1107 5 0.7631242158522831 1107 6 0.9598314824540743 1107 7 -0.3435573242930694 1107 8 -0.9894047594249962 1107 9 -0.821373469457793 1107 10-11 -0.419741327077471 1107 12-13 0.55469117854323 1107 14-15 -1.8999451716394766 1107 16-17 -0.20311987176843616 1107 18-19 1.3083965722745141 1107 20-21 0.44336331146892505 1107 22-23 -0.4663819216054357 1107 24-25 -0.3335428960528688 1107 26-27 0.05324852568420724 1107 28-29 -1.443290770201095 1107 30-31 0.28708683894704023 1107 32-33 1.165558921933421 1107 34-35 0.09665144949701698 1107 36-37 0.24442129580566885 1107 38-39 0.7332000575490838 1107 40-41 0.8595167514740965 1107 42-43 -0.11283249462528033 1107 44-45 0.5077318353423976 1107 46-47 0.5321107638544653 1107 48-49 -0.08883755998968468 1107 50-51 -0.5721442587261087 1107 52-53 1.1827218419209622 1107 54-55 0.2732897564734422 1107 56-57 0.5009420541568339 1107 58-59 -0.24757426683432016 1107 60-61 -0.7493753686288329 1107 62-63 -0.2877123425615764 1107 64-65 0.12092631838482504 1107 66-67 0.5822864265913523 1107 68-69 1.568336790494783 1107 70-71 0.18466797196392548 1107 72-73 0.15788235384350102 1107 74-75 -0.2312609914402124 1107 76 -0.8234226248262324 1108 1 -1.3296642406613124 1108 2 -0.09197405495467947 1108 3 -0.49941805999215205 1108 4 -0.21805605146349905 1108 5 0.8418643733325979 1108 6 -1.1425307222703331 1108 7 -0.6506439384662954 1108 8 1.0499653193151595 1108 9 -0.837121500953856 1108 10-11 -0.89218227195936 1108 12-13 -0.4098757505906292 1108 14-15 0.24178711182509005 1108 16-17 -0.9393403442093806 1108 18-19 -0.23884752221367478 1108 20-21 0.7032058311539693 1108 22-23 -0.5687441263298432 1108 24-25 -1.1760625810922392 1108 26-27 -1.0333656475441408 1108 28-29 -0.28974746311448385 1108 30-31 0.8776380200494032 1108 32-33 0.03957466996491732 1108 34-35 -0.521458786723457 1108 36-37 -0.3697519325407903 1108 38-39 0.3080032071553802 1108 40-41 -0.7074123823841703 1108 42-43 1.0407108124613345 1108 44-45 -0.0788823378859469 1108 46-47 0.9927406851143026 1108 48-49 -0.2502548828243292 1108 50-51 0.9317927491479061 1108 52-53 -0.45901044154360093 1108 54-55 0.45045511080415324 1108 56-57 0.3710207943143118 1108 58-59 -1.9129285975429795 1108 60-61 0.34511282034754487 1108 62-63 -0.6499170669710246 1108 64-65 -0.5956091146860416 1108 66-67 0.14527855257560418 1108 68-69 0.8872344282900606 1108 70-71 1.4130144286568367 1108 72-73 -0.4326688272588619 1108 74-75 1.0994476699771063 1108 76 0.04271910745723417 1109 1 -0.38401415423918905 1109 2 -0.9845545555628341 1109 3 1.0789700073530888 1109 4 1.2052843236725366 1109 5 0.9143309247697644 1109 6 0.8260372311257349 1109 7 0.34353057020792477 1109 8 1.3046865279445683 1109 9 2.03042859186991 1109 10-11 2.0023827366828577 1109 12-13 0.0028699122990687442 1109 14-15 1.061837044607877 1109 16-17 -0.19434962658566945 1109 18-19 0.5279407755905616 1109 20-21 1.2843145949975145 1109 22-23 1.1251615135139552 1109 24-25 1.1020566174225763 1109 26-27 0.5480311900462809 1109 28-29 1.3568500658529352 1109 30-31 1.320631426361416 1109 32-33 1.7754110440766873 1109 34-35 1.2488824206256197 1109 36-37 0.7463417874515308 1109 38-39 0.8484615644948548 1109 40-41 0.6980034040571574 1109 42-43 1.667815351223254 1109 44-45 1.2711272645722822 1109 46-47 1.4953013406421185 1109 48-49 1.476139074028623 1109 50-51 2.1889145723346424 1109 52-53 1.8130272000926588 1109 54-55 0.2328313991339641 1109 56-57 0.5634540572296203 1109 58-59 0.8681673630229199 1109 60-61 1.5560468194513142 1109 62-63 0.025743902237092442 1109 64-65 0.10098521346196776 1109 66-67 1.0694511407581828 1109 68-69 2.296235132470329 1109 70-71 0.39793856923682824 1109 72-73 -0.16565647209594303 1109 74-75 1.4258672333853397 1109 76 0.6339744688297451 1110 1 -0.09640439814162605 1110 2 0.05687633874611109 1110 3 -0.27485113085829127 1110 4 -0.38177258689657023 1110 5 -0.8104820833603164 1110 6 0.29400471080053237 1110 7 0.25448991980141855 1110 8 1.0912881539608286 1110 9 -1.4662868552845651 1110 10-11 -1.7880562877073878 1110 12-13 -0.2728048844488953 1110 14-15 -0.2867483212457742 1110 16-17 0.3491950888614852 1110 18-19 0.6664123203060122 1110 20-21 -0.6768886570350112 1110 22-23 -0.24934255152669493 1110 24-25 -0.4132279354229489 1110 26-27 -1.4393184034496507 1110 28-29 -0.06562147886251424 1110 30-31 -0.3404092240450858 1110 32-33 0.23160616602790896 1110 34-35 0.5075490872922899 1110 36-37 0.8093661776954342 1110 38-39 -0.23209128103359689 1110 40-41 -0.13994781545503798 1110 42-43 0.6772462455322028 1110 44-45 1.4433223865235014 1110 46-47 0.30600052763399077 1110 48-49 0.5671309439473227 1110 50-51 0.7722966861557801 1110 52-53 0.28883207814143574 1110 54-55 0.6853842446624192 1110 56-57 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2.063458340767699 2116 54-55 1.6992024264805998 2116 56-57 0.7870312930019807 2116 58-59 -0.3773364556516299 2116 60-61 0.08869032770457963 2116 62-63 1.9283061096773473 2116 64-65 1.5384082240557078 2116 66-67 0.8696482336382019 2116 68-69 1.3483415626255422 2116 70-71 -1.6423422881172023 2116 72-73 2.217812362592408 2116 74-75 -0.6778528947250315 2116 76 0.2454551290908924 2117 1 -0.19142326606615612 2117 2 0.5860084142178081 2117 3 1.248167737066236 2117 4 0.6557368470656932 2117 5 -1.8298783097754097 2117 6 -1.048636798633428 2117 7 -2.21207611793443 2117 8 -2.3345986384920003 2117 9 0.5894867296210968 2117 10-11 -0.7821317594055053 2117 12-13 0.24489322875865227 2117 14-15 -0.8379558684155874 2117 16-17 -0.2369558545347381 2117 18-19 -0.4260782457317269 2117 20-21 0.3364698335310301 2117 22-23 0.9403555616808532 2117 24-25 0.7599796117468571 2117 26-27 0.9801910305126107 2117 28-29 0.7070955022695635 2117 30-31 0.2744587004832155 2117 32-33 1.3630778641411148 2117 34-35 0.9155490872922911 2117 36-37 -1.0646338223045682 2117 38-39 0.2856068321739471 2117 40-41 -1.5465893248889984 2117 42-43 -1.294640546920629 2117 44-45 1.179548801617841 2117 46-47 0.1309816597094624 2117 48-49 -0.8604539617130555 2117 50-51 1.471051403136915 2117 52-53 0.6263037762546446 2117 54-55 -0.8552572647715415 2117 56-57 -1.4236605308973935 2117 58-59 -0.457572781170974 2117 60-61 0.575873479982075 2117 62-63 -0.8580956437055356 2117 64-65 -0.6456016863692966 2117 66-67 -1.789537968000829 2117 68-69 -1.5244428856306058 2117 70-71 0.3459367284637018 2117 72-73 0.8236823835567648 2117 74-75 -0.9238029614298178 2117 76 -0.07234551889843388 2118 1 -1.2308343046836825 2118 2 0.22637166584891233 2118 3 -0.960084775718105 2118 4 -0.49511838128909247 2118 5 -0.5266689992481659 2118 6 0.8263411593986625 2118 7 0.6990506674649701 2118 8 -3.2523380142634686 2118 9 0.42963837836029484 2118 10-11 -0.20379460546439887 2118 12-13 1.1867465174202607 2118 14-15 -0.8485800969467121 2118 16-17 -0.7041133223534679 2118 18-19 -1.4998306703481958 2118 20-21 -2.9848138906798702 2118 22-23 -0.421529467414544 2118 24-25 -0.6289288700023903 2118 26-27 0.6494292601017513 2118 28-29 -0.405098114376532 2118 30-31 -0.8612316539516272 2118 32-33 0.12405476415875327 2118 34-35 0.42022198448855264 2118 36-37 -0.529587093332605 2118 38-39 -0.01085763617378177 2118 40-41 -0.961330993025129 2118 42-43 -0.45770702549583575 2118 44-45 0.7646307977384552 2118 46-47 -0.7448779770389073 2118 48-49 -0.36023354203398483 2118 50-51 0.7087079011090509 2118 52-53 -0.8019716601763207 2118 54-55 0.39121602036335545 2118 56-57 0.29481945499574636 2118 58-59 1.0952397739339474 2118 60-61 -1.0686777455513443 2118 62-63 0.5559847653933865 2118 64-65 -0.14789405103161357 2118 66-67 -2.0101780667488462 2118 68-69 0.7894347373635782 2118 70-71 -0.8418608381030417 2118 72-73 -0.7204015522570266 2118 74-75 0.8120460951739581 2118 76 0.12329898824316388 2119 1 0.17855212359750183 2119 2 0.7135719909200233 2119 3 0.06297495609823045 2119 4 0.36205350005995385 2119 5 0.2874309601179448 2119 6 1.0848742760179242 2119 7 -1.0258579062855375 2119 8 0.021635980047786774 2119 9 -1.2642868552845634 2119 10-11 0.6434219731621731 2119 12-13 1.0666733764206668 2119 14-15 -1.387791799506644 2119 16-17 -1.3912831720080803 2119 18-19 -0.17084854925920823 2119 20-21 -1.1224103961654457 2119 22-23 0.4903965789080864 2119 24-25 0.4891633689248778 2119 26-27 0.21911637915904336 2119 28-29 0.22411765157226782 2119 30-31 -0.7118005283929136 2119 32-33 0.7713018182018239 2119 34-35 0.36663604381403303 2119 36-37 0.07667052552151787 2119 38-39 -0.0013086723379451826 2119 40-41 -0.22286085893329854 2119 42-43 -0.6231885370764942 2119 44-45 -1.847112396085194 2119 46-47 -0.6186081680181807 2119 48-49 -0.9781734038787633 2119 50-51 0.9437749470253429 2119 52-53 -1.5198200957716104 2119 54-55 -1.7217461901201894 2119 56-57 0.5732631770599497 2119 58-59 0.6335880063597905 2119 60-61 0.4349793044283423 2119 62-63 0.6495950864011064 2119 64-65 0.38085442519756185 2119 66-67 -0.12085462099508959 2119 68-69 -0.24931983394889912 2119 70-71 -1.1886202854821555 2119 72-73 -1.7411248354752296 2119 74-75 0.34465479082613015 2119 76 -1.3340354286605347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 21.0 13 158.0 14 483.0 15 830.0 16 1082.0 17 1337.0 18 1331.0 19 1236.0 20 1094.0 21 932.0 22 791.0 23 680.0 24 662.0 25 674.0 26 807.0 27 817.0 28 975.0 29 1157.0 30 1359.0 31 1731.0 32 1979.0 33 2530.0 34 3368.0 35 4653.0 36 6676.0 37 5116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.88377603487904 21.088548207473405 14.998933725090632 29.028742032556927 2 18.63245851476992 22.63622454984112 29.13028127574438 29.60103565964458 3 19.792868071083912 23.876662351418148 28.160527244909968 28.169942332587972 4 11.745321878309992 24.314463928445335 31.870071790043546 32.07014240320113 5 11.582911615864424 31.928916088031066 30.803813110509594 25.684359185594918 6 26.271066754542886 33.98455889275962 17.698427643348083 22.0459467093494 7 22.577380251853597 30.78262916323408 18.345298340590794 28.294692244321524 8 19.399788160527244 32.44439213840179 18.02283158761916 30.13298811345181 9 20.786159821113333 20.854419206778864 18.597151935977404 39.7622690361304 10-11 20.07061315758503 26.049193833117574 25.70789690478993 28.17229610450747 12-13 20.78262916323408 23.988466517594446 25.840885018241732 29.38801930092974 14-15 19.97528539484524 26.096269271507587 22.96457573261151 30.96386960103566 16-17 21.096857714487466 27.992232552665648 24.034365070024716 26.876544662822173 18-19 19.649287983994352 27.667412027774507 24.35212427915735 28.33117570907379 20-21 19.21619395080617 27.556784747557963 24.8217017770978 28.40531952453807 22-23 21.81005060609627 27.31787689772861 24.3485936212781 26.523478874897023 24-25 20.48605390137696 29.073790749676355 23.850770860303637 26.589384488643052 26-27 18.99023184653407 29.899964693421204 25.520772037189598 25.589031422855125 28-29 19.942332587972224 27.55443097563846 27.60268329998823 24.900553136401083 30-31 19.983523596563494 27.83217606213958 27.001294574555722 25.1830057667412 32-33 18.48887842768036 29.8787807461457 26.312816288101683 25.319524538072262 34-35 19.189125573731904 28.885488996116276 26.010356596445806 25.915028833706018 36-37 19.72343179945863 27.794515711427564 26.34929975285395 26.132752736259857 38-39 19.409203248205248 26.86948334706367 27.78039307991056 25.940920324820528 40-41 18.909026715311285 27.082499705778513 26.9495115923267 27.0589619865835 42-43 17.78863128162881 26.316346945980936 28.23467106037425 27.660350712016008 44-45 17.706249264446274 26.624691067435563 29.116158644227376 26.552901023890783 46-47 17.825114746381075 27.58503001059197 28.18524185006473 26.404613392962222 48-49 17.497940449570436 28.212310227138993 27.230787336707074 27.0589619865835 50-51 17.196657643874307 27.85336000941509 26.87419089090267 28.07579145580793 52-53 16.888313522419676 27.300223608332352 27.6438743085795 28.16758856066847 54-55 16.909497469695186 27.936918912557374 26.436389313875488 28.717194303871956 56-57 17.18135812639755 27.420265976226904 26.692950453101094 28.70542544427445 58-59 17.74390961515829 27.16958926680005 26.617629751677065 28.468871366364603 60-61 16.89184418029893 26.635283041073322 26.861245145345414 29.611627633282332 62-63 17.36730610803813 27.015417206072733 26.139814052018362 29.477462633870775 64-65 18.192303165823233 27.469695186536423 24.812286689419796 29.52571495822055 66-67 17.657996940096503 28.89372719783453 24.1155702012475 29.332705660821468 68-69 17.308461810050606 28.45239496292809 25.158291161586444 29.080852065434858 70-71 17.946334000235378 28.918441802989292 24.665175944450983 28.470048252324347 72-73 17.679180887372013 29.219724608685414 24.784041426385784 28.317053077556785 74-75 17.58032246675297 29.71519359774038 23.92962221960692 28.77486171589973 76 17.476756502294926 28.60774390961516 24.370954454513356 29.544545133576555 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 3.5 14 4.0 15 2.5 16 8.0 17 12.0 18 13.5 19 20.0 20 27.5 21 30.0 22 36.5 23 44.5 24 66.5 25 87.0 26 112.5 27 170.5 28 217.5 29 232.0 30 290.0 31 400.0 32 525.5 33 599.0 34 684.0 35 860.5 36 1075.5 37 1199.0 38 1368.5 39 1699.0 40 1995.5 41 2256.0 42 2381.0 43 2490.0 44 2689.5 45 2869.0 46 2958.0 47 2906.5 48 2829.5 49 2677.0 50 2550.0 51 2366.5 52 2106.0 53 1853.0 54 1677.0 55 1570.5 56 1347.5 57 1100.5 58 970.0 59 879.0 60 716.5 61 575.0 62 505.0 63 459.5 64 352.0 65 250.0 66 174.0 67 138.0 68 113.5 69 72.5 70 48.5 71 41.0 72 34.5 73 27.5 74 24.0 75 21.0 76 13.5 77 7.5 78 7.0 79 5.0 80 4.5 81 4.5 82 5.0 83 5.0 84 4.0 85 3.0 86 2.5 87 2.0 88 2.0 89 2.0 90 2.5 91 2.0 92 1.0 93 0.5 94 1.0 95 2.5 96 3.0 97 2.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6637636812992821 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0023537719195010002 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 42485.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.49535130045899 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82172356440108 73.32705660821468 2 7.958165365207341 13.289396257502647 3 2.5145885603134777 6.2986936565846765 4 0.9415611873819525 3.144639284453336 5 0.3890282750260762 1.6241026244556902 6 0.16068559185859668 0.8049899964693421 7 0.10712372790573112 0.6261033305872661 8 0.031009500183237955 0.20713192891608803 9 0.031009500183237955 0.233023420030599 >10 0.04510472753925521 0.4448628927856891 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016476403436507 0.0 2 0.0 0.0 0.0 0.016476403436507 0.0 3 0.0 0.0 0.0 0.016476403436507 0.0 4 0.0 0.0 0.0 0.016476403436507 0.0 5 0.0 0.0 0.0 0.018830175356008002 0.0 6 0.0 0.0 0.0 0.018830175356008002 0.0 7 0.0 0.0 0.0 0.018830175356008002 0.0 8 0.0 0.0 0.0 0.018830175356008002 0.0 9 0.0 0.0 0.0 0.032952806873014 0.0 10 0.0 0.0 0.0 0.032952806873014 0.0 11 0.0 0.0 0.0 0.032952806873014 0.0 12 0.0 0.0 0.0 0.037660350712016004 0.0 13 0.0 0.0 0.0 0.042367894551018005 0.0 14 0.0 0.0 0.0 0.04707543839002001 0.0 15 0.0 0.0 0.0 0.04707543839002001 0.0 16 0.0 0.0 0.0 0.05884429798752501 0.0 17 0.0 0.0 0.0 0.06355184182652701 0.0 18 0.0 0.0 0.0 0.06825938566552901 0.0 19 0.0 0.0 0.0 0.07532070142403201 0.0 20 0.0 0.0 0.0 0.07767447334353302 0.0 21 0.0 0.0 0.0 0.07767447334353302 0.0 22 0.0 0.0 0.0 0.08238201718253502 0.0 23 0.0 0.0 0.0 0.09650464869954102 0.0 24 0.0 0.0 0.0 0.10121219253854302 0.0 25 0.0 0.0 0.0 0.10591973637754501 0.0 26 0.0 0.0 0.0 0.12004236789455101 0.0 27 0.0 0.0 0.0 0.14358008708956102 0.0 28 0.0 0.0 0.0 0.17182535012357303 0.0 29 0.0 0.0 0.0 0.19536306931858302 0.0 30 0.0 0.0 0.0 0.24479227962810404 0.0 31 0.0 0.0 0.0 0.289513946098623 0.0 32 0.0 0.0 0.0 0.34835824408614807 0.0 33 0.0 0.0 0.0 0.4260327174296811 0.0 34 0.0 0.0 0.0 0.48487701541720607 0.0 35 0.0 0.0 0.0 0.5884429798752501 0.0 36 0.0 0.0 0.0 0.7484994704013181 0.0 37 0.0 0.0 0.0 1.0309521007414382 0.0 38 0.0 0.0 0.0 1.3110509591620572 0.0 39 0.0 0.0 0.0 1.7088384135577264 0.0 40 0.0 0.0 0.0 2.1537013063434154 0.0 41 0.0 0.0 0.0 2.7068377074261503 0.0 42 0.0 0.0 0.0 3.3776627044839356 0.0 43 0.0 0.0 0.0 4.083794280334236 0.0 44 0.0 0.0 0.0 4.691067435565493 0.0 45 0.0 0.0 0.0 5.420736730610804 0.0 46 0.0 0.0 0.0 6.018594798164058 0.0 47 0.0 0.0 0.0 6.583500058844298 0.0 48 0.0 0.0 0.0 7.167235494880546 0.0 49 0.0 0.0 0.0 7.758032246675297 0.0 50 0.0 0.0 0.0 8.25703189360951 0.0 51 0.0 0.0 0.0 8.878427680357774 0.0 52 0.0 0.0 0.0 9.504531010945039 0.0 53 0.0 0.0 0.0 10.13769565729081 0.0 54 0.0 0.0 0.0 10.721431093327057 0.0 55 0.0 0.0 0.0 11.262798634812286 0.0 56 0.0 0.0 0.0 11.820642579734024 0.0 57 0.0 0.0 0.0 12.392609156172767 0.0 58 0.0 0.0 0.0 12.908085206543486 0.0 59 0.0 0.0 0.0 13.378839590443686 0.0 60 0.0 0.0 0.0 13.95315993880193 0.0 61 0.0 0.0 0.0 14.402730375426621 0.0 62 0.0 0.0 0.0 14.859362127809815 0.0 63 0.0 0.0 0.0 15.36542309050253 0.0 64 0.0 0.0 0.0 15.95151229845828 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATG 15 0.0022114608 69.9894 2 TTACCCC 25 2.3665809E-4 55.991516 2 TTTGTGA 20 0.0069103744 52.492046 33 TCTGTAT 20 0.0069103744 52.492046 53 AATTCTT 20 0.0069103744 52.492046 39 TGAACCG 80 0.0026277856 21.871687 70 CCCTCCT 85 0.0037351113 20.585117 9 TTGAACC 85 0.0037351113 20.585117 69 AATTGAA 110 7.323683E-4 19.088018 67 ACCCTGT 95 0.0071058474 18.418262 54 GACGAAT 180 6.904191E-5 15.553201 70 CGACGAA 240 7.8992685E-4 11.6649 69 CCGACGA 285 0.003310353 9.823073 68 GCCGACG 300 0.005057114 9.33192 67 TCTGACG 520 0.007862893 6.72975 59 >>END_MODULE