FastQCFastQC Report
Wed 25 May 2016
SRR1780474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42485
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT18834.432152524420384No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT16673.9237377898081673TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC6161.4499235024126162No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC2990.703777803930799No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1720.40484877015417203No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1540.36248087560315406No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1230.289513946098623No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT830.19536306931858305No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT800.18830175356008003No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC800.18830175356008003No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC770.18124043780157703No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG670.157702718606567No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT660.15534894668706603No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT570.13416499941155702No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG550.129457455572555No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC480.11298105213604802No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC460.10827350829704602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGA150.002210133270.029
AGACGGA150.002210133270.024
ACGGAGT150.002210133270.026
GACGGAG150.002210133270.025
CATACCT150.002210133270.050
AGTCGAA150.002210133270.030
TTTACAC950.062.6315772
CTATACA601.8189894E-1258.3333321
GAGACTT200.00690623452.50000421
CCCCGCT200.00690623452.50000416
GGAGTCG200.00690623452.50000428
CGAGACT200.00690623452.50000420
GCCGTAT200.00690623452.50000443
GCGTAGA200.00690623452.50000427
CCCGCTA200.00690623452.50000417
TGCCGTA200.00690623452.50000442
CTTTACA1150.051.739131
TTACACA1150.051.739133
CCTCGTA451.6474623E-646.66666435
ACCTCGT451.6474623E-646.66666434
Produced by FastQC (version 0.11.5)