FastQCFastQC Report
Wed 25 May 2016
SRR1780473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482795
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT250.002349573435.2055635
GGTCATA551.36768E-732.0083731
AACCTTG407.070706E-427.4701565
GGGTCTA655.905258E-727.084011
AAACTCG1001.7498678E-924.18626427
ACGAAAC1301.8189894E-1223.70588324
CTATACA1355.456968E-1222.8207841
ACCTTAA500.002588282321.98523532
CCTTAAG755.4528973E-520.5387081
ACGAAGA654.8764303E-420.3383218
CGAGTGC654.921009E-420.310922
CGGCCGA1101.2417877E-720.03016518
CCGCCTA550.004446203420.02600515
AGTCTAT550.00447567920.00315940
CGAAACT1101.2664896E-719.9927925
ACGCCTT808.942025E-519.25903310
CGGTGGT1950.019.16264243
GACGAAA2900.018.96851323
CGACGAA4100.018.78345522
CATAAGA600.00733729518.36289221
Produced by FastQC (version 0.11.5)