FastQCFastQC Report
Wed 25 May 2016
SRR1780473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482795
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT280585.8115763419256625No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT237214.913265464638201RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC75341.5604966911422034No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC38460.7966113982124918No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG34360.7116892262761627No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT19940.4130117337586346RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC15530.32166861711492456No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC12610.2611874605163682No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG10190.21106266634907156No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG8410.1741940160937872No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC8370.17336550709928644No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC6090.12614049441274247No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC5390.11164158700897897No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5040.10439213330709722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT26350.042.41126644
CTTATAA1100.040.039211
TTTACAC10150.039.0125772
GCCGTCT29000.038.53575543
CTATACA5050.038.374211
CTTTACA10650.037.2195471
TTACACA11500.034.432843
TCGAAGT353.2121813E-431.43330613
GGCTTAT502.3396224E-630.8301931
CTTATGC502.3586144E-630.798263
AAGGTCG300.00573741229.33775311
ATGCGGA602.9406328E-729.3316786
TTCGGCA300.00574322429.3316785
CGGTAAG300.00574322429.33167829
CTTATAC45050.028.2052041
TTATACA45400.027.7649522
TCTTTAT2400.027.5269571
CTTATAT905.0567905E-1026.9152451
CATTAGT508.3344465E-526.39850842
TCCGTAT609.725767E-625.67053413
Produced by FastQC (version 0.11.5)