FastQCFastQC Report
Wed 25 May 2016
SRR1780472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135362
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTA150.002221444269.9937329
CTTGGTC200.00694132252.4952925
CGGGCGC200.00694132252.4952912
GCATGTA200.00694132252.495292
CGCCCTG200.00694132252.4952959
TGTCCTA200.00694132252.4952928
TGATATA305.863528E-446.66248345
GCTCGGA305.863528E-446.66248311
ATGCAGG350.001252400639.9964131
GTGATAT501.7034358E-434.99686444
GTCGCCC400.002412987234.996869
AGTTTAG400.002412987234.9968644
CACGGTT400.002412987234.9968649
GGTCCAT400.002412987234.9968622
TGTTCGT400.002412987234.9968610
CAAGAGT400.002412987234.996865
ACGGTTA400.002412987234.9968650
CGAAATT450.004297060431.10831820
GACGTTG450.004297060431.10831825
GCACCTA450.004297060431.10831825
Produced by FastQC (version 0.11.5)