##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780472_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135362 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.13068660333033 34.0 27.0 34.0 12.0 34.0 2 30.156742660421685 34.0 31.0 34.0 12.0 34.0 3 30.27958363499357 34.0 32.0 34.0 11.0 34.0 4 30.41974113857656 34.0 32.0 34.0 11.0 34.0 5 30.593911141974854 34.0 32.0 34.0 21.0 34.0 6 33.41636500642721 38.0 34.0 38.0 21.0 38.0 7 32.672286165984545 37.0 32.0 38.0 11.0 38.0 8 31.74397541407485 37.0 27.0 38.0 11.0 38.0 9 32.465174864437586 37.0 31.0 38.0 12.0 38.0 10-11 32.66570012263412 37.0 32.5 38.0 11.0 38.0 12-13 32.95145609550686 37.0 33.5 38.0 11.0 38.0 14-15 32.592252626290986 37.0 31.5 38.0 11.0 38.0 16-17 32.44092138118527 37.0 31.0 38.0 11.0 38.0 18-19 32.4415530207887 37.0 32.0 38.0 11.0 38.0 20-21 32.50016622094827 37.0 31.5 38.0 11.0 38.0 22-23 32.437611737415224 37.0 31.5 38.0 11.0 38.0 24-25 32.79790118349315 37.0 33.0 38.0 11.0 38.0 26-27 32.805480858734356 37.5 33.5 38.0 11.0 38.0 28-29 32.891380151002494 37.0 34.0 38.0 11.0 38.0 30-31 32.735091827839426 37.0 34.0 38.0 11.0 38.0 32-33 32.57054047664781 37.0 32.0 38.0 11.0 38.0 34-35 32.48512507202908 37.0 31.5 38.0 11.0 38.0 36-37 32.4468277655472 37.0 31.5 38.0 11.0 38.0 38-39 32.824932403481036 37.0 34.0 38.0 11.0 38.0 40-41 32.46327625182843 37.0 31.5 38.0 11.0 38.0 42-43 32.73993070433357 37.0 33.5 38.0 11.0 38.0 44-45 31.9185295725536 37.0 29.0 38.0 11.0 38.0 46-47 32.56609314283182 37.0 32.0 38.0 11.0 38.0 48-49 32.37951566909473 37.0 31.0 38.0 11.0 38.0 50-51 32.31481508842955 37.0 31.0 38.0 11.0 38.0 52-53 32.31496653418242 37.0 31.0 38.0 11.0 38.0 54-55 32.065989716464 37.0 31.0 38.0 11.0 38.0 56-57 32.09564722743458 37.0 31.0 38.0 11.0 38.0 58-59 31.97203055510409 37.0 29.0 38.0 11.0 38.0 60-61 32.04148505488985 37.0 31.0 38.0 11.0 38.0 62-63 32.07731859753845 37.0 31.0 38.0 11.0 38.0 64-65 32.11027097708367 37.0 31.0 38.0 11.0 38.0 66-67 32.12148165659491 37.0 31.0 38.0 11.0 38.0 68-69 31.669962766507588 37.0 29.0 38.0 11.0 38.0 70-71 31.546829981826512 37.0 27.5 38.0 11.0 38.0 72-73 31.66035150189861 37.0 28.5 38.0 11.0 38.0 74-75 31.34736484390006 37.0 27.5 38.0 11.0 38.0 76 30.754657880350468 36.0 26.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.455849269993365 1101 2 -0.7219761480436446 1101 3 -0.9747731666733834 1101 4 -1.5789106638348969 1101 5 -1.3044027991271143 1101 6 -2.7219922553265192 1101 7 -1.1832833697559515 1101 8 -1.1890391863672747 1101 9 -0.8227054054479801 1101 10-11 -1.2821774321667334 1101 12-13 -1.4347479603588802 1101 14-15 -0.9400636079676197 1101 16-17 -2.366134774277697 1101 18-19 -0.24066370240647217 1101 20-21 -1.649104285120515 1101 22-23 -0.904530316146662 1101 24-25 -1.5027720829233395 1101 26-27 -1.8563008840064548 1101 28-29 -0.8078283196782081 1101 30-31 -1.2965870114357685 1101 32-33 -0.6055232460593913 1101 34-35 -1.0864289023447427 1101 36-37 -0.47228274974055395 1101 38-39 -1.5400935762029135 1101 40-41 -0.5335752006589516 1101 42-43 -1.0785576900127296 1101 44-45 -0.28266545228311557 1101 46-47 -0.1535518063281529 1101 48-49 -1.3207023140641923 1101 50-51 -1.1414872556377489 1101 52-53 -0.5191836119787538 1101 54-55 -1.7690531280398432 1101 56-57 -1.3625525230213498 1101 58-59 -0.2882622166766815 1101 60-61 -0.5217282890202384 1101 62-63 -2.3194248972794256 1101 64-65 -1.5318461445581661 1101 66-67 -1.9567336777123678 1101 68-69 -1.3104149338090565 1101 70-71 -0.5614689742014072 1101 72-73 -0.9495468044859621 1101 74-75 -1.4577878411162608 1101 76 -1.918350918894319 1102 1 0.9127241763343186 1102 2 0.20245888020494363 1102 3 0.22522683332661586 1102 4 0.6270391949221654 1102 5 0.2915541217768407 1102 6 0.18992511755483577 1102 7 0.8941883816564768 1102 8 0.9918047401863994 1102 9 1.094543888337327 1102 10-11 -0.4404754547655969 1102 12-13 -3.5761762795679175 1102 14-15 -1.0559622661597103 1102 16-17 -2.1761453674980373 1102 18-19 -0.7502682221804768 1102 20-21 0.14955390696987791 1102 22-23 -0.735850937615588 1102 24-25 -0.07600654620017622 1102 26-27 -4.7495653614075835 1102 28-29 -0.21820967561041726 1102 30-31 0.17625903376197272 1102 32-33 -5.740374940974647 1102 34-35 -0.7809733938701697 1102 36-37 0.08475291410125863 1102 38-39 0.09171256786488158 1102 40-41 0.34767479934104983 1102 42-43 -0.22916680018222735 1102 44-45 -5.118258672622101 1102 46-47 -0.23863302101741368 1102 48-49 -0.2015109298834048 1102 50-51 -5.594682877106678 1102 52-53 -1.589743287120001 1102 54-55 0.12035365162117273 1102 56-57 -0.763753087993102 1102 58-59 0.31949731722162156 1102 60-61 0.34865483244868756 1102 62-63 -4.161232806883945 1102 64-65 -0.6244838564225716 1102 66-67 0.05879421776786131 1102 68-69 -6.82311797053222 1102 70-71 0.014678624668647444 1102 72-73 0.3455450034236449 1102 74-75 -5.677959098178405 1102 76 -1.4205225290638097 1103 1 -0.24301770500662911 1103 2 -0.05453582974125837 1103 3 0.07535945932131227 1103 4 0.7117474926637755 1103 5 0.5228103971593576 1103 6 -0.7782671227005267 1103 7 0.6262988583607552 1103 8 0.9989830284868368 1103 9 0.2700747669658128 1103 10-11 0.5374014803001117 1103 12-13 -1.4336745187143194 1103 14-15 -0.35671231486417554 1103 16-17 -0.47023789098857094 1103 18-19 0.17259889706303255 1103 20-21 -0.4233416856510175 1103 22-23 -0.30809463975408846 1103 24-25 0.4747809674745369 1103 26-27 -0.5729330126536709 1103 28-29 0.5750960835048105 1103 30-31 0.49835662251648216 1103 32-33 -4.2895252354493145 1103 34-35 -0.4791593400105256 1103 36-37 0.08118624893318582 1103 38-39 0.5634002898448358 1103 40-41 0.4332185659193044 1103 42-43 0.3903539479182996 1103 44-45 -4.554283489418399 1103 46-47 0.021025448313764628 1103 48-49 0.3091965586148504 1103 50-51 0.3251158610731295 1103 52-53 -0.5259516822705308 1103 54-55 0.7765039011378789 1103 56-57 0.6486875300290436 1103 58-59 0.34837156979546435 1103 60-61 1.1808454841893052 1103 62-63 0.6321666146569171 1103 64-65 0.6670348236115942 1103 66-67 0.16378605040434024 1103 68-69 -2.9720487202812045 1103 70-71 0.3035260523237895 1103 72-73 1.4131355562567407 1103 74-75 -1.063975672681245 1103 76 1.2401122773921536 1104 1 -1.2050701372020391 1104 2 0.1252460741785768 1104 3 -0.17992221816389886 1104 4 -0.297194877927037 1104 5 -0.4968570674197963 1104 6 7.973788198185616E-4 1104 7 0.6137694757725001 1104 8 1.448926599540581 1104 9 0.2091458818148908 1104 10-11 -0.5669165920583339 1104 12-13 -0.7136986717409926 1104 14-15 -0.689017706883611 1104 16-17 -1.0045409395893543 1104 18-19 0.25960730030356416 1104 20-21 0.8940190211125483 1104 22-23 0.46215328818938417 1104 24-25 0.6290368601660887 1104 26-27 0.38297333686075063 1104 28-29 -0.47676463404135205 1104 30-31 -0.7002624856905157 1104 32-33 -0.7072000753276839 1104 34-35 0.30277333342760926 1104 36-37 0.16591592912123332 1104 38-39 0.06418741973204334 1104 40-41 0.30634688606191673 1104 42-43 0.4361907855970202 1104 44-45 -2.6642372680012762 1104 46-47 0.02528457578567611 1104 48-49 0.2386388105970525 1104 50-51 0.5355706711915218 1104 52-53 0.004016760649975026 1104 54-55 -0.6783349708582733 1104 56-57 0.19310465855046033 1104 58-59 0.892289104461522 1104 60-61 0.11271022723992274 1104 62-63 0.13518214879103851 1104 64-65 0.1246663893171771 1104 66-67 0.04150057025510989 1104 68-69 -3.9780258755434765 1104 70-71 -0.6010946517081805 1104 72-73 0.033329211774201895 1104 74-75 -1.2514743248560976 1104 76 -0.5898532901680298 1105 1 -0.2615853442639242 1105 2 0.2186737193303614 1105 3 -0.4392294000951331 1105 4 -0.07074587603649007 1105 5 0.6607414316421156 1105 6 0.6222633812782519 1105 7 1.4883678238780007 1105 8 0.6912907207945302 1105 9 1.1135760932257597 1105 10-11 1.3185685890534273 1105 12-13 -0.030491494841641043 1105 14-15 0.7042956426955058 1105 16-17 0.8851997747939251 1105 18-19 0.9802912047553392 1105 20-21 0.41220208092723 1105 22-23 0.896149392076147 1105 24-25 0.5822080231774578 1105 26-27 0.5517354223595916 1105 28-29 -0.3201293806861685 1105 30-31 0.36970940766237703 1105 32-33 1.1282466478398092 1105 34-35 1.1097000764350966 1105 36-37 1.394183596413292 1105 38-39 0.7901907407732196 1105 40-41 1.0897172396593504 1105 42-43 0.911574107069498 1105 44-45 1.7335149190696733 1105 46-47 1.318107676430472 1105 48-49 0.8529631368641901 1105 50-51 0.7588028637256485 1105 52-53 -0.15459889712464303 1105 54-55 -0.22349609886212463 1105 56-57 0.7043904478009253 1105 58-59 0.8364352302729223 1105 60-61 0.4726226725182201 1105 62-63 0.580442476725878 1105 64-65 0.7824194389962074 1105 66-67 1.023202496027686 1105 68-69 1.522646239930996 1105 70-71 1.534295283093023 1105 72-73 1.0112787923310194 1105 74-75 -0.5241878742727586 1105 76 -0.2903917013876871 1106 1 -0.12419722320974103 1106 2 0.38255601569904485 1106 3 -0.6431942193049629 1106 4 -0.2237388802091651 1106 5 0.535235358767622 1106 6 0.0958622768372237 1106 7 0.7999768641621756 1106 8 1.2855404919952953 1106 9 0.6038114074175169 1106 10-11 0.7525813397630898 1106 12-13 -0.013905489598641907 1106 14-15 0.1536224300440736 1106 16-17 0.547662740342183 1106 18-19 -0.44885083690939 1106 20-21 -0.0828031155298774 1106 22-23 0.4993805025667868 1106 24-25 0.48034195216438036 1106 26-27 0.3687813528356543 1106 28-29 -0.6508838752337667 1106 30-31 -0.3487434441843149 1106 32-33 0.5488261691452863 1106 34-35 0.23270486958507774 1106 36-37 0.4252227327155822 1106 38-39 -0.1187394095362393 1106 40-41 -0.03536613633146146 1106 42-43 0.6032496930282036 1106 44-45 0.549498290406941 1106 46-47 0.14584512349641443 1106 48-49 0.22558789061417173 1106 50-51 0.4270982706780373 1106 52-53 -0.05371394823606579 1106 54-55 -0.013058975993061495 1106 56-57 0.593368529610224 1106 58-59 -0.2193568804193724 1106 60-61 0.8020198834943812 1106 62-63 0.8691715939486429 1106 64-65 0.6826823642137683 1106 66-67 0.881323705328569 1106 68-69 1.0986037065418195 1106 70-71 0.48712386205590263 1106 72-73 0.2489546575023418 1106 74-75 -1.0840944931630432 1106 76 -0.5398604218182967 1107 1 0.01994860234706053 1107 2 0.07057704344571647 1107 3 0.30767364183725476 1107 4 -0.17897715319659824 1107 5 0.08156794555373637 1107 6 0.5825955106309237 1107 7 -0.27982592294743824 1107 8 0.48203794129230104 1107 9 -0.379686788426703 1107 10-11 -0.39693807046460705 1107 12-13 0.021003369428356677 1107 14-15 0.01741644522386565 1107 16-17 0.6505540555095379 1107 18-19 0.8521554465297001 1107 20-21 0.7495659276454418 1107 22-23 -0.789237762955171 1107 24-25 -0.5166018701573805 1107 26-27 -0.031134660602198494 1107 28-29 -0.8001155537207651 1107 30-31 -0.35230509654215325 1107 32-33 0.0860990943661406 1107 34-35 0.1323875870169573 1107 36-37 -0.2538319518682144 1107 38-39 0.4393279663502838 1107 40-41 0.17480245891552215 1107 42-43 0.3080447036042884 1107 44-45 0.05589837750411775 1107 46-47 0.022711491544193052 1107 48-49 0.26048119657410496 1107 50-51 0.5381005103196941 1107 52-53 0.6675517603616754 1107 54-55 0.5711064464282387 1107 56-57 0.380532583361628 1107 58-59 0.4423561343871505 1107 60-61 -0.6165753634883231 1107 62-63 -0.5055951100453839 1107 64-65 0.6279277916120449 1107 66-67 0.6246825456918899 1107 68-69 0.8036475661909428 1107 70-71 0.1142624087773143 1107 72-73 0.120532982748081 1107 74-75 -0.07783438366945461 1107 76 -0.29687485506453015 1108 1 -0.7284288455901837 1108 2 -0.3118302594494793 1108 3 -0.8715901428007058 1108 4 -0.5508519768322451 1108 5 0.2071605297853516 1108 6 0.22703791708727294 1108 7 0.5838585400583725 1108 8 1.3305480152242346 1108 9 -0.4328430049174763 1108 10-11 -0.2371343287184544 1108 12-13 -0.18168512879388743 1108 14-15 0.29315505914113515 1108 16-17 -1.0060469095562112 1108 18-19 -0.347295002141216 1108 20-21 -0.030768741353938367 1108 22-23 -0.6555747458548851 1108 24-25 -0.8700466187323563 1108 26-27 -0.452243357481251 1108 28-29 0.2567936962369046 1108 30-31 -0.4950120777487683 1108 32-33 0.3537108388212431 1108 34-35 -0.05740867688055573 1108 36-37 -0.16417184125248951 1108 38-39 0.18143742512334882 1108 40-41 0.19051299562752178 1108 42-43 0.12244943863447588 1108 44-45 -0.22934980241574365 1108 46-47 0.056834466881397816 1108 48-49 -0.40300503289708445 1108 50-51 -0.47329262699053487 1108 52-53 -0.4490286053474577 1108 54-55 -0.2699151970053606 1108 56-57 0.6009421719388612 1108 58-59 -1.1184719315307952 1108 60-61 0.5827022481150408 1108 62-63 -0.2590800696932192 1108 64-65 -0.4045832135236793 1108 66-67 0.02585501592158579 1108 68-69 0.6897549932466482 1108 70-71 1.165595017841035 1108 72-73 0.2155228241612548 1108 74-75 0.619048199997799 1108 76 -0.2691715422364922 1109 1 -0.6833360073938977 1109 2 -0.84233423822932 1109 3 -0.5426776759572043 1109 4 -0.6940880511028027 1109 5 -0.3074283296310938 1109 6 -0.35651645957054967 1109 7 -1.180067189384598 1109 8 0.022855707531928005 1109 9 0.20906680940613143 1109 10-11 -0.23580806877151161 1109 12-13 -0.37929786088938044 1109 14-15 -0.3832375138031594 1109 16-17 -0.7315111005376451 1109 18-19 -0.7783294848998352 1109 20-21 -0.15013213657940838 1109 22-23 -0.19005750447557546 1109 24-25 -0.12336226859972754 1109 26-27 -0.43765449806480916 1109 28-29 -0.8280869403678679 1109 30-31 -0.6090704331864387 1109 32-33 -0.11843770229281603 1109 34-35 -0.22451743019620096 1109 36-37 -0.5620498253373754 1109 38-39 -0.1355387021976 1109 40-41 -0.24582652691889706 1109 42-43 0.009717343143762491 1109 44-45 -0.3155566989674661 1109 46-47 -1.3993989548692625 1109 48-49 -0.7000872610137954 1109 50-51 0.16331214754526968 1109 52-53 0.21277510818039502 1109 54-55 -0.6306579025756527 1109 56-57 -0.05423024185424197 1109 58-59 -0.8068008439976389 1109 60-61 -0.18652852111572926 1109 62-63 -0.9606715816295583 1109 64-65 -0.7189068209507354 1109 66-67 -0.529847901850296 1109 68-69 -0.006531478901894872 1109 70-71 -1.281354584280983 1109 72-73 -1.6438936214620874 1109 74-75 0.20127631670867174 1109 76 -1.4601529745972357 1110 1 0.12101243213429314 1110 2 -0.1927208288947071 1110 3 -0.6045604007159362 1110 4 -0.09653034468595934 1110 5 0.31295092427714266 1110 6 0.4708933829713544 1110 7 0.5260251408823464 1110 8 1.790548579590169 1110 9 -1.5366016820437238 1110 10-11 -0.7267253045071556 1110 12-13 -0.3021349284439836 1110 14-15 -0.2086473845633705 1110 16-17 -0.003701263639399599 1110 18-19 0.6553469358914086 1110 20-21 -0.12144471065243323 1110 22-23 0.38097500300226983 1110 24-25 0.6576002575021889 1110 26-27 -1.2133155116660284 1110 28-29 -0.0487857664867235 1110 30-31 -0.014539139095347764 1110 32-33 0.748333136919328 1110 34-35 0.43823865084674196 1110 36-37 -0.6022362071873673 1110 38-39 0.14677477486092272 1110 40-41 0.29980245891552215 1110 42-43 0.4197468312638648 1110 44-45 0.9707919945253956 1110 46-47 0.04132851282079031 1110 48-49 0.6142046008294173 1110 50-51 0.7189515741494859 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0.7212691868791623 2116 38-39 0.525861529430891 2116 40-41 0.7582381796227367 2116 42-43 0.7527143170295219 2116 44-45 1.7905739843366 2116 46-47 0.4814393908549519 2116 48-49 0.9557501507245405 2116 50-51 0.7752821105594307 2116 52-53 1.2252045922465982 2116 54-55 0.2496088437911439 2116 56-57 0.3341024065561129 2116 58-59 -0.24052894202879216 2116 60-61 -1.2015918453582657 2116 62-63 0.7291666520163531 2116 64-65 1.1712788554418339 2116 66-67 0.47797142792143177 2116 68-69 0.5689644675993968 2116 70-71 -1.8953158051873231 2116 72-73 0.5683229138238985 2116 74-75 -0.9066214678768247 2116 76 -1.128782256922488 2117 1 0.07932514861128581 2117 2 -0.012673822462250683 2117 3 0.7513896240242914 2117 4 0.27247015011858977 2117 5 -0.44212082238292894 2117 6 -0.9044777204427987 2117 7 -1.8655508116164157 2117 8 -2.0221060468323913 2117 9 -0.2771385449828614 2117 10-11 -0.3369739437946393 2117 12-13 0.08563721405973013 2117 14-15 -0.2340679684327327 2117 16-17 0.05675766758276524 2117 18-19 -0.4409544000808836 2117 20-21 -0.46276707581818854 2117 22-23 0.11044061408589556 2117 24-25 0.8204837310301443 2117 26-27 1.1047819648307566 2117 28-29 0.6517647035776051 2117 30-31 0.06663973275027502 2117 32-33 0.9248546609173474 2117 34-35 -0.06469925118194908 2117 36-37 -1.2949935055545083 2117 38-39 0.11430322612267219 2117 40-41 -1.2046507820543013 2117 42-43 -0.3401492597801763 2117 44-45 0.25570664074014005 2117 46-47 -0.07077564353745203 2117 48-49 -0.5700482442967498 2117 50-51 0.7501551862227132 2117 52-53 1.2937887717421717 2117 54-55 0.042284081262479845 2117 56-57 -0.5439478718585598 2117 58-59 -0.5406968096999378 2117 60-61 0.4965493272588333 2117 62-63 -0.45314582751198174 2117 64-65 -0.23918626083722927 2117 66-67 -1.610040363758877 2117 68-69 0.5129498917723403 2117 70-71 1.3532839327753337 2117 72-73 0.9709473815605492 2117 74-75 0.14253919376746182 2117 76 0.010646174128936536 2118 1 0.14595590711834916 2118 2 0.04471322525335708 2118 3 -0.04861512852624372 2118 4 0.3081380418871724 2118 5 0.4163482226712496 2118 6 0.48016203622661635 2118 7 0.528126711987916 2118 8 -3.2154952490375734 2118 9 -0.9345667814697798 2118 10-11 -0.26945604252095734 2118 12-13 0.5066221009490235 2118 14-15 -0.6122323749975926 2118 16-17 -0.08555745820140004 2118 18-19 -0.9305819585372568 2118 20-21 -2.4693018328044403 2118 22-23 -0.36285797282241106 2118 24-25 -0.18625300935385525 2118 26-27 0.4479438367019952 2118 28-29 -0.14914303357466707 2118 30-31 -0.08880431388808319 2118 32-33 -0.028360439519886427 2118 34-35 0.8979971875735089 2118 36-37 -0.23534899633455808 2118 38-39 -0.10420716612116365 2118 40-41 -1.08352411074069 2118 42-43 -0.2594628330645037 2118 44-45 0.39063155044167885 2118 46-47 -0.20420746027910042 2118 48-49 -0.9773695483966165 2118 50-51 -0.3838152529129566 2118 52-53 -0.7559981555754831 2118 54-55 0.1026771171917602 2118 56-57 0.7746749974146141 2118 58-59 0.5360608419064228 2118 60-61 -0.9071719198104304 2118 62-63 0.1553707430475484 2118 64-65 -0.15188190750094321 2118 66-67 -1.9698084392927484 2118 68-69 0.893395522594215 2118 70-71 -0.342608279378517 2118 72-73 -0.925245033368796 2118 74-75 0.5896789504368698 2118 76 -0.35681311235480706 2119 1 0.1665669311239526 2119 2 0.7354260865932254 2119 3 0.7112603528796875 2119 4 0.76108584454499 2119 5 0.8936593517108733 2119 6 1.2831928005394033 2119 7 -0.7797219172419858 2119 8 1.2418441935209934 2119 9 0.18097825376989363 2119 10-11 0.44963122705114955 2119 12-13 0.640959092713743 2119 14-15 -0.09616521411287238 2119 16-17 0.39690642683962096 2119 18-19 0.2515150685432488 2119 20-21 -0.45615735774621413 2119 22-23 1.094736443629877 2119 24-25 0.4889178612107372 2119 26-27 0.9371435992337638 2119 28-29 0.897688440098328 2119 30-31 0.7424143852122782 2119 32-33 0.845838485784185 2119 34-35 0.7286374384373744 2119 36-37 0.23378394020357973 2119 38-39 0.44085090703730856 2119 40-41 0.38957424068183144 2119 42-43 0.4363951731157556 2119 44-45 -0.4603190835680344 2119 46-47 0.3786933054037007 2119 48-49 -0.19924630847760483 2119 50-51 1.2261279678259314 2119 52-53 0.12317846902683272 2119 54-55 -0.299919049827551 2119 56-57 1.014053622230044 2119 58-59 1.255985339189234 2119 60-61 0.890972479136181 2119 62-63 0.151245493782028 2119 64-65 0.874072151531216 2119 66-67 1.1669831518965736 2119 68-69 0.2781587393753 2119 70-71 0.48760574646897936 2119 72-73 -0.6506990391228342 2119 74-75 0.8871231225708911 2119 76 0.926603690044228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 8.0 12 47.0 13 311.0 14 1002.0 15 1836.0 16 2453.0 17 2900.0 18 3152.0 19 2928.0 20 2612.0 21 2051.0 22 1651.0 23 1519.0 24 1429.0 25 1502.0 26 1670.0 27 1831.0 28 2226.0 29 2704.0 30 3262.0 31 4113.0 32 5334.0 33 7057.0 34 9677.0 35 14734.0 36 25015.0 37 32337.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.66190582459273 26.793126534255745 10.431451312950978 28.11351632820055 2 17.217535201903043 26.903414547657395 32.54532291189551 23.33372733854405 3 16.00744669848259 25.950414444231022 28.506523248769966 29.535615608516423 4 13.5407278261255 21.117448028250173 35.835020168141725 29.506803977482605 5 11.15527252847919 38.786365449683075 34.10188974749191 15.95647227434583 6 23.19410174199554 41.50500140364357 17.750180996143676 17.550715858217224 7 23.08772033510143 32.50690740385041 23.3883955615313 21.01697669951685 8 20.508709977689456 36.109838802618164 21.18393640755899 22.19751481213339 9 21.732096156971675 19.643622286904744 21.51711706387317 37.10716449225041 10-11 21.965174864437582 26.802573839039024 27.60302004994016 23.629231246583235 12-13 21.763493447200837 24.647611589663274 26.331614485601573 27.25728047753432 14-15 20.34802972769315 26.1048152361815 23.390981220726644 30.156173815398706 16-17 21.355328674221717 28.258299966017052 24.951241855173535 25.4351295045877 18-19 20.472510748954655 27.676896026949954 25.58362021837739 26.266973005718004 20-21 20.026669227700538 27.99456272809208 25.625729525272973 26.35303851893441 22-23 21.866919815014555 27.66692277005363 25.410381052289416 25.055776362642394 24-25 21.018084839171998 28.8363794861187 25.18838374137498 24.957151933334316 26-27 19.756725509099706 29.11056688940356 26.279628993473032 24.853078608023697 28-29 20.642425496077184 27.713834015454854 28.15302669877809 23.490713789689867 30-31 20.60925743287419 27.682908360205822 27.467189710515918 24.240644496404073 32-33 19.40130908231261 29.335411710819876 26.705796309156188 24.55748289771132 34-35 20.838196835153145 28.304103071763127 26.699886230995407 24.157813862088325 36-37 21.29992169146437 27.475214609713213 26.578360248814292 24.646503450008126 38-39 20.57224331791788 27.05929285914806 28.006382884413643 24.36208093852041 40-41 20.401220431140203 26.721679644213292 27.6237053235029 25.253394601143604 42-43 19.338883881739335 26.841728106854212 28.078411961998196 25.740976049408253 44-45 19.18632998921411 26.971011066621358 28.247587949350628 25.595070994813906 46-47 19.433814512196925 27.243613421787504 28.267165083258227 25.055406982757344 48-49 18.995729968528835 27.79251193097029 27.815782863728373 25.395975236772507 50-51 19.06221834783765 27.659535172352655 27.21295489132844 26.06529158848126 52-53 18.66859730646198 27.469507465222627 27.63351334579384 26.22838188252155 54-55 18.194544997857598 27.77625921602813 27.528774693045317 26.500421093068955 56-57 18.84102939166602 27.628609316533876 27.240020242092474 26.29034104970763 58-59 19.014198962781283 27.308254901671074 26.846530045359852 26.83101609018779 60-61 18.518491157045553 26.82473663214196 26.96953354708116 27.68723866373133 62-63 18.345251990957582 26.971749826391456 27.462655693621546 27.22034248902942 64-65 19.297143954728803 27.492944844195566 25.76203070285605 27.44788049821959 66-67 18.92007697905239 28.3090095780558 25.48287363836837 27.288039804523446 68-69 18.297971365671312 28.502460070034424 26.05310205227464 27.146466512019625 70-71 19.205168363351603 28.896219027496638 25.543357810907047 26.355254798244708 72-73 19.309702870820466 28.42415153440404 25.313603522406584 26.952542072368907 74-75 18.975783454736188 28.70007830853563 25.102318228158566 27.221820008569615 76 19.342947060474874 27.513630117758307 25.475391912058036 27.66803090970878 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.5 11 4.0 12 5.0 13 4.5 14 7.5 15 11.5 16 13.0 17 14.0 18 18.5 19 34.0 20 46.0 21 47.0 22 65.0 23 103.5 24 143.5 25 163.0 26 251.0 27 437.5 28 605.0 29 674.0 30 856.5 31 1258.0 32 1804.0 33 2131.0 34 2353.5 35 3021.5 36 3861.0 37 4255.0 38 4870.0 39 6116.5 40 7357.5 41 8386.0 42 8805.0 43 9054.5 44 9688.5 45 10192.0 46 10311.0 47 10241.5 48 9702.5 49 8750.0 50 8267.0 51 7690.5 52 6378.0 53 5227.5 54 4813.0 55 4428.5 56 3463.5 57 2542.0 58 2201.0 59 1844.0 60 1344.5 61 1005.0 62 808.0 63 662.5 64 425.0 65 299.5 66 227.5 67 189.0 68 152.0 69 98.0 70 68.0 71 55.0 72 44.0 73 32.5 74 29.5 75 27.0 76 18.5 77 12.5 78 14.5 79 14.0 80 11.5 81 7.5 82 6.5 83 7.0 84 6.0 85 6.5 86 7.0 87 6.0 88 5.0 89 3.5 90 4.5 91 3.0 92 0.0 93 0.5 94 4.0 95 6.5 96 6.0 97 5.5 98 4.5 99 6.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6885241057312983 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.6937988504897977E-4 28-29 0.0 30-31 3.6937988504897977E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.387597700979595E-4 54-55 0.0 56-57 3.6937988504897977E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.6937988504897977E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135362.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.3959013607955 #Duplication Level Percentage of deduplicated Percentage of total 1 79.27985597119424 43.917790812783494 2 7.8629059145162365 8.711455208995138 3 4.0928185637127426 6.801761203291913 4 2.2991264919650596 5.094487374595529 5 1.4016136560645462 3.8821825918647774 6 1.0215376408615056 3.3953399033702225 7 0.7361472294458892 2.8545677516585157 8 0.5534440221377609 2.4526824367252256 9 0.46942721877708876 2.3403909516703356 >10 2.2711208908448355 20.12307737769832 >50 0.01200240048009602 0.4262643873465226 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004432558620587757 0.0 2 0.0 0.0 0.0 0.005171318390685717 0.0 3 0.0 0.0 0.0 0.005171318390685717 0.0 4 0.0 0.0 0.0 0.006648837930881636 0.0 5 0.0 0.0 0.0 0.006648837930881636 0.0 6 0.0 0.0 0.0 0.006648837930881636 0.0 7 0.0 0.0 0.0 0.006648837930881636 0.0 8 0.0 0.0 0.0 0.006648837930881636 0.0 9 0.0 0.0 0.0 0.011820156321567353 0.0 10 0.0 0.0 0.0 0.011820156321567353 0.0 11 0.0 0.0 0.0 0.014775195401959191 0.0 12 0.0 0.0 0.0 0.014775195401959191 0.0 13 0.0 0.0 0.0 0.015513955172057151 0.0 14 0.0 0.0 0.0 0.018468994252448988 0.0 15 0.0 0.0 0.0 0.018468994252448988 0.0 16 0.0 0.0 0.0 0.019946513792644907 0.0 17 0.0 0.0 0.0 0.022162793102938786 0.0 18 0.0 0.0 0.0 0.023640312643134705 0.0 19 0.0 0.0 0.0 0.028811631033820423 0.0 20 0.0 0.0 0.0 0.030289150574016342 0.0 21 0.0 0.0 0.0 0.03250542988431022 0.0 22 0.0 0.0 0.0 0.03324418965440818 0.0 23 0.0 0.0 0.0 0.03398294942450614 0.0 24 0.0 0.0 0.0 0.03767674827499594 0.0 25 0.0 0.0 0.0 0.039893027585289814 0.0 26 0.0 0.0 0.0 0.043586826435779616 0.0 27 0.0 0.0 0.0 0.057623262067640846 0.0 28 0.0 0.0 0.0 0.06722713907891432 0.0 29 0.0 0.0 0.0 0.08865117241175514 0.0 30 0.0 0.0 0.0 0.11229148505488985 0.0 31 0.0 0.0 0.0 0.14184187585880823 0.0 32 0.0 0.0 0.0 0.16991474712253068 0.0 33 0.0 0.0 0.0 0.22015041148919196 0.0 34 0.0 0.0 0.0 0.27112483562595113 0.0 35 0.0 0.0 0.0 0.34795585171613896 0.0 36 0.0 0.0 0.0 0.5075279620572982 0.0 37 0.0 0.0 0.0 0.711425658604335 0.0 38 0.0 0.0 0.0 1.025398560895968 0.0 39 0.0 0.0 0.0 1.373354412612107 0.0 40 0.0 0.0 0.0 1.7442118172012824 0.0 41 0.0 0.0 0.0 2.1653048861571196 0.0 42 0.0 0.0 0.0 2.62850726200854 0.0 43 0.0 0.0 0.0 3.1301251459050548 0.0 44 0.0 0.0 0.0 3.5719034884236343 0.0 45 0.0 0.0 0.0 4.106765561974557 0.0 46 0.0 0.0 0.0 4.643105155065676 0.0 47 0.0 0.0 0.0 5.1757509493063045 0.0 48 0.0 0.0 0.0 5.664809917111154 0.0 49 0.0 0.0 0.0 6.1760316780189415 0.0 50 0.0 0.0 0.0 6.6732170032948686 0.0 51 0.0 0.0 0.0 7.170402328570796 0.0 52 0.0 0.0 0.0 7.674975251547702 0.0 53 0.0 0.0 0.0 8.243081514753033 0.0 54 0.0 0.0 0.0 8.755042035430918 0.0 55 0.0 0.0 0.0 9.260353718177923 0.0 56 0.0 0.0 0.0 9.68883438483474 0.0 57 0.0 0.0 0.0 10.116576291721458 0.0 58 0.0 0.0 0.0 10.59381510320474 0.0 59 0.0 0.0 0.0 11.019340730781165 0.0 60 0.0 0.0 0.0 11.461857833069843 0.0 61 0.0 0.0 0.0 11.93688036524283 0.0 62 0.0 0.0 0.0 12.431110651438365 0.0 63 0.0 0.0 0.0 12.86033007786528 0.0 64 0.0 0.0 0.0 13.31245105716523 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTGTA 15 0.0022214442 69.99373 29 CTTGGTC 20 0.006941322 52.49529 25 CGGGCGC 20 0.006941322 52.49529 12 GCATGTA 20 0.006941322 52.49529 2 CGCCCTG 20 0.006941322 52.49529 59 TGTCCTA 20 0.006941322 52.49529 28 TGATATA 30 5.863528E-4 46.662483 45 GCTCGGA 30 5.863528E-4 46.662483 11 ATGCAGG 35 0.0012524006 39.99641 31 GTGATAT 50 1.7034358E-4 34.996864 44 GTCGCCC 40 0.0024129872 34.99686 9 AGTTTAG 40 0.0024129872 34.99686 44 CACGGTT 40 0.0024129872 34.99686 49 GGTCCAT 40 0.0024129872 34.99686 22 TGTTCGT 40 0.0024129872 34.99686 10 CAAGAGT 40 0.0024129872 34.99686 5 ACGGTTA 40 0.0024129872 34.99686 50 CGAAATT 45 0.0042970604 31.108318 20 GACGTTG 45 0.0042970604 31.108318 25 GCACCTA 45 0.0042970604 31.108318 25 >>END_MODULE