FastQCFastQC Report
Wed 25 May 2016
SRR1780472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135362
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT47163.483991075781977No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT41563.0702856045271196No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC13210.9759016562994046No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC5970.4410395827484819No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG5060.37381244366956756No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT3680.2718635953960491No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC2660.19651009884605725No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC2480.18321242298429397No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1890.13962559654851436No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1580.11672404367547762No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1440.10638140689410618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA1150.054.782611
AATCTCG200.006938860352.533
ACGTCTC407.4349373E-752.558
CTCGAGT407.4349373E-752.562
CCGCCCA200.006938860352.525
TTTACAC2800.051.252
CGAGTGA451.6747108E-646.66666864
GTCTCGA451.6747108E-646.66666860
CGTCTGC305.860938E-446.66666438
GCTTGAC305.860938E-446.66666453
CTTGACA305.860938E-446.66666454
ATATCGT551.3011595E-744.54545234
CTTTACA3150.044.4444471
TCTCGAG503.4581008E-642.061
ACGAACC503.4581008E-642.021
CGTTATA602.5771988E-740.83333230
TCTGCCG350.001251848240.040
CGAACCT350.001251848240.022
GTCTGCC350.001251848240.039
CTGCCGT350.001251848240.041
Produced by FastQC (version 0.11.5)