FastQCFastQC Report
Wed 25 May 2016
SRR1780471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691272
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTGGTC49300.7131780254371651No Hit
CTTTACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTGG11000.15912694279531067No Hit
CTATACACATCTGACGCTGCCGACGAGCCACGTCGTGTAGATCTCGGTGG9800.14176763994491315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT9000.037.35423743
GGTGGTC8750.034.15244744
GTCGTTT401.8288076E-532.97393830
CGTCGTT608.067218E-932.97155429
GTATTAA353.2245275E-431.4151211
AACCGTA901.6370905E-1129.35692610
GCATTCG300.005755353729.3207821
CGAATCT300.005775753429.2995632
TGTACAC300.005790067429.2847273
TCGGTGG13550.028.5406842
TATCGGC953.092282E-1127.85019315
ACGTCGT13600.027.79551928
CAACCGT953.274181E-1127.7434239
AGCCACG14100.027.00678324
CACGTCG14100.026.96378127
CGTCGTG13350.026.83826629
GCCACGT14100.026.81374225
GTATCGG1006.002665E-1126.44999914
CCACGTC14600.025.88984126
CTCGGTG14800.025.83321841
Produced by FastQC (version 0.11.5)