FastQCFastQC Report
Wed 25 May 2016
SRR1780471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691272
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT287974.165798701524147No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT240453.478370308648405RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC65560.9483965790600516No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG37100.5366917797914569No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC35340.5112314689442071No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT21110.3053790693099098RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC18490.2674779247532086No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC16230.23478457105162656No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG9550.138151118517747No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC9420.1362705273756206No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC7560.10936360795750442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG12550.040.84186624
CGTCTTC12700.040.70591718
TCTTCTG12550.040.6665820
CTTGAAA12700.040.3594827
GTCTTCT13100.039.46298619
TTGAAAA13350.039.2183328
CGTGCGA452.3455868E-839.11696610
CTTCTGC13550.037.9900621
CAGTAGT301.2944434E-436.6960451
TCTGCTT14200.036.25107623
TTCTGCT14350.036.02544422
GCTTGAA14350.035.25894526
GACTTAT250.002342872835.2282071
CCGTGCG505.9319063E-835.205279
CGAGAAT505.9319063E-835.2052714
CTATACA5750.034.8452871
TGCTTGA14500.034.74248525
TGAAAAA15100.034.23610729
TTTACAC8600.033.5142172
CTTTATA2300.033.4808462
Produced by FastQC (version 0.11.5)