FastQCFastQC Report
Wed 25 May 2016
SRR1780470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113684
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC150.00222111969.98920
GCGGAAT150.00222111969.98919
GGTCCCA200.006808359652.7470171
GTCAAGC200.00692824652.5148548
TACTTCG200.0069403252.49175329
GAATCGG200.0069403252.49175322
ACTTCGA200.0069403252.49175330
GTAGGTC200.0069403252.49175331
AGCGGAA200.0069403252.49175318
CGTAGCG200.0069403252.49175315
TGTTCGA200.0069403252.49175337
GAGTTCC200.0069403252.49175315
ACTTTCG200.0069403252.49175365
GTACCCT404.4436085E-543.955851
GCAGGCC350.001251967739.99371339
GGTAGCA350.001251967739.99371319
TAACCGT459.1344016E-538.899897
AGGGACC459.158134E-538.88278246
CAGTAAC400.002355388135.1646771
GTGCAAA501.6979914E-435.00990312
Produced by FastQC (version 0.11.5)