FastQCFastQC Report
Wed 25 May 2016
SRR1780470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113684
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT43313.8096829808944093No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT39223.4499137961366597Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC12761.1224094859434925No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC6570.5779177368846979No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG5220.45916751697688324No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT3380.2973153653988248No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC2650.23310228352274726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC2580.22694486471271244No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1890.1662503078709405No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT1450.1275465324935787No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1420.12490763871784948No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1300.11435206361493262No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA207.897963E-570.0000110
CATCGTT150.002219735270.032
GCTAATC150.002219735270.014
TTTACAC2050.061.4634172
ATCTCTT200.00693600952.50000437
TCGTTGT200.00693600952.50000434
CCGTGCG200.00693600952.5000049
TGACGAT200.00693600952.50000442
GTAATCG200.00693600952.50000421
ACGATTC200.00693600952.50000424
GCATTTC200.00693600952.50000454
CTTATAA407.424278E-752.5000041
GGTGCTA200.00693600952.50000437
GAACGAA200.00693600952.50000467
GGTGCAC200.00693600952.5000041
GATCTCT200.00693600952.50000436
CCAACGA200.00693600952.50000416
GCATGTA200.00693600952.5000042
CTATACA1300.051.153851
GCGAGAA352.0755095E-550.013
Produced by FastQC (version 0.11.5)